Restriction map for
pFN21AB6333
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 726 GTAC GT^AC
AflIII 545 ACGTGT A^C[GA][CT]GT
Alw44I 163 GTGCAC G^TGCAC
AlwI 755 GGATC GGATC(4/5)
AlwNI 380 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 642 GGGCCC GGGCC^C
ApaLI 163 GTGCAC G^TGCAC
Asp700I 321 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 627 CTCGGG C^[CT]CG[GA]G
AviII 552 TGCGCA TGC^GCA
BalI 773 TGGCCA TGG^CCA
BcnI 9 CCCGG CC^[GC]GG
BglI 708 GCCCGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 348 CTCCAG CTGGAG(16/14)
BsaWI 746 ACCGGA [AT]^CCGG[AT]
BseRI 487 GAGGAG GAGGAG(10/8)
BsgI 50 CTGCAC GTGCAG(16/14)
BsmAI 66 GTCTC GTCTC(1/5)
BsmI 99 GCATTC GAATGC(1/-1)
BsoBI 627 CTCGGG C^[CT]CG[GA]G
Bsp1407I 725 TGTACA T^GTACA
BspDI 323 ATCGAT AT^CGAT
BsrBI 295 CCGCTC CCGCTC(-3/-3)
BsrGI 725 TGTACA T^GTACA
BssSI 131 CTCGTG CACGAG(-5/-1)
BstEII 573 GGTGACC G^GT[ACGT]ACC
BstPI 573 GGTGACC G^GT[ACGT]ACC
CfoI 553 GCGC GCG^C
ClaI 323 ATCGAT AT^CGAT
EaeI 773 TGGCCA [CT]^GGCC[GA]
EcoO65I 573 GGTGACC G^GT[ACGT]ACC
EcoT14I 731 CCAAGG C^C[AT][AT]GG
FauI 294 CCCGC CCCGC(4/6)
FspI 552 TGCGCA TGC^GCA
HgaI 599 GACGC GACGC(5/10)
HhaI 553 GCGC GCG^C
HinP1I 553 GCGC G^CGC
MluNI 773 TGGCCA TGG^CCA
MscI 773 TGGCCA TGG^CCA
MspA1I 185 CAGCTG C[AC]G^C[GT]G
NciI 9 CCCGG CC^[GC]GG
PleI 346 GACTC GAGTC(4/5)
PvuII 185 CAGCTG CAG^CTG
RsaI 726 GTAC GT^AC
SacI 391 GAGCTC GAGCT^C
SspBI 725 TGTACA T^GTACA
StuI 715 AGGCCT AGG^CCT
StyI 731 CCAAGG C^C[AT][AT]GG
TaqI 324 TCGA T^CGA
TthHB8I 324 TCGA T^CGA
XmnI 321 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 330 CCTCAGG CC^T[ACGT]AGG
461 CCTCAGG
AspHI 163 GTGCAC G[AT]GC[AT]^C
391 GAGCTC
BanII 391 GAGCTC G[GA]GC[CT]^C
642 GGGCCC
BbsI 250 GAAGAC GAAGAC(2/6)
16 GTCTTC
BpuAI 250 GAAGAC GAAGAC(2/6)
16 GTCTTC
BsiHKAI 163 GTGCAC G[AT]GC[AT]^C
391 GAGCTC
BsmFI 445 GGGAC GGGAC(10/14)
7 GTCCC
BsrI 441 ACTGG ACTGG(1/-1)
171 CCAGT
Bsu36I 330 CCTCAGG CC^T[ACGT]AGG
461 CCTCAGG
Cac8I 384 GCCTGC GC[ACGT]^[ACGT]GC
435 GCCAGC
DpnII 216 GATC ^GATC
756 GATC
Eco81I 330 CCTCAGG CC^T[ACGT]AGG
461 CCTCAGG
HapII 10 CCGG C^CGG
747 CCGG
HpaII 10 CCGG C^CGG
747 CCGG
MaeIII 34 GTGAC ^GT[ACGT]AC
574 GTGAC
MboI 216 GATC ^GATC
756 GATC
MslI 729 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
765 CACCATCGTG
MspI 10 CCGG C^CGG
747 CCGG
NdeII 216 GATC ^GATC
756 GATC
PpuMI 5 GGGTCCC [GA]G^G[AT]CC[CT]
176 GGGTCCT
Sau3AI 216 GATC ^GATC
756 GATC
SfaNI 400 GATGC GCATC(5/9)
197 GCATC
Tsp45I 34 GTGAC ^GT[GC]AC
574 GTGAC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BspMI
BssKI BstF5I BstNI Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MboII MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TfiI TseI
TspRI