(Circular) (Six-base) MAPSORT of: pFN21AB6333.seq Check: 4179 from: 1 to: 5488 pFN21AB6333 5488 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2110 2465 2636 2110 Size: 355 171 4962 Fragments arranged by size: 4962 355 171 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2342 2501 3551 4935 5203 1632 Size: 642 68 159 1050 1384 268 1917 Fragments arranged by size: 1917 1384 1050 642 268 159 68 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 2550 4387 5364 1203 Size: 1347 1837 977 1327 Fragments arranged by size: 1837 1347 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 2738 2774 1166 Size: 1572 36 3880 Fragments arranged by size: 3880 1572 36 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 2390 3231 4803 2390 Size: 841 1572 3075 Fragments arranged by size: 3075 1572 841 ApaI G_GGCC'C Cuts at: 2651 2651 Size: 5488 ApaLI G'TGCA_C Cuts at: 2168 3545 4701 2168 Size: 1377 1156 2955 Fragments arranged by size: 2955 1377 1156 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2632 2813 1870 Size: 73 689 181 4545 Fragments arranged by size: 4545 689 181 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2809 4264 618 Size: 325 217 627 1022 1455 1842 Fragments arranged by size: 1842 1455 1022 627 325 217 BanII G_rGCy'C Cuts at: 1391 2400 2651 2807 1391 Size: 1009 251 156 4072 Fragments arranged by size: 4072 1009 251 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2014 2262 928 Size: 971 115 248 4154 Fragments arranged by size: 4154 971 248 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2719 4099 136 Size: 107 122 71 631 605 1047 1380 1525 Fragments arranged by size: 1525 1380 1047 631 605 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 2172 2651 2710 3549 4705 1790 Size: 382 479 59 839 1156 2573 Fragments arranged by size: 2573 1156 839 479 382 59 BmgBI CAC'GTC Cuts at: 1318 2043 2551 2685 1318 Size: 725 508 134 4121 Fragments arranged by size: 4121 725 508 134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2170 2456 3219 Size: 416 53 83 399 24 753 286 763 Cuts at: 3219 4177 442 Size: 958 1753 Fragments arranged by size: 1753 958 763 753 416 399 286 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2338 4148 1239 Size: 1099 1810 2579 Fragments arranged by size: 2579 1810 1099 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3613 4478 4776 3613 Size: 865 298 4325 Fragments arranged by size: 4325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3279 493 Size: 711 2075 2702 Fragments arranged by size: 2702 2075 711 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 3677 4312 275 Size: 53 83 186 936 1762 382 635 1451 Fragments arranged by size: 1762 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2751 3921 4319 4593 4740 5151 2751 Size: 1170 398 274 147 411 3088 Fragments arranged by size: 3088 1170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5263 Fragments arranged by size: 5263 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 1186 Size: 1321 4167 BseYI C'CCAG_C Cuts at: 2088 2227 2472 4691 5187 2088 Size: 139 245 2219 496 2389 Fragments arranged by size: 2389 2219 496 245 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 1163 Size: 573 304 4611 Fragments arranged by size: 4611 573 304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3699 4727 1540 Size: 275 186 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2400 3549 3634 4705 2172 Size: 228 1149 85 1071 2955 Fragments arranged by size: 2955 1149 1071 228 85 BsmI GAATG_Cn' Cuts at: 2104 2999 3092 2104 Size: 895 93 4500 Fragments arranged by size: 4500 895 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2400 2651 2710 2807 3549 3634 Size: 399 382 228 251 59 97 742 85 Cuts at: 3634 4705 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 251 228 97 85 59 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2110 2433 2465 2636 2843 844 Size: 1266 323 32 171 207 3489 Fragments arranged by size: 3489 1266 323 207 171 32 BsrBI CCG'CTC Cuts at: 1394 1823 2302 3375 1394 Size: 429 479 1073 3507 Fragments arranged by size: 3507 1073 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4132 4319 1700 Size: 110 2322 187 2869 Fragments arranged by size: 2869 2322 187 110 BsrGI T'GTAC_A Cuts at: 96 2730 96 Size: 2634 2854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 2136 3548 4560 2136 Size: 1412 1012 3064 Fragments arranged by size: 3064 1412 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 2578 1184 Size: 1394 4094 BstYI r'GATC_y Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483 Size: 846 367 385 17 1452 86 12 346 Cuts at: 5483 1972 Size: 1977 Fragments arranged by size: 1977 1452 846 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2336 2467 2336 Size: 131 5357 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4936 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 2197 2701 3004 3798 3818 2197 Size: 504 303 794 20 3867 Fragments arranged by size: 3867 794 504 303 20 ClaI AT'CG_AT Cuts at: 1554 2329 1554 Size: 775 4713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2778 2935 3824 Size: 54 1425 50 275 18 948 157 889 Cuts at: 3824 5453 8 Size: 1629 43 Fragments arranged by size: 1629 1425 948 889 275 157 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3418 1353 Size: 2065 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2366 4075 4449 4595 387 Size: 1064 138 777 1709 374 146 1280 Fragments arranged by size: 1709 1280 1064 777 374 146 138 EcoICRI GAG'CTC Cuts at: 2398 2398 Size: 5488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2274 2338 2342 2501 3551 4148 4935 Size: 393 642 64 4 159 1050 597 787 Cuts at: 4935 5203 1239 Size: 268 1524 Fragments arranged by size: 1524 1050 787 642 597 393 268 159 64 4 EcoO109I rG'GnC_Cy Cuts at: 2011 2182 2647 2011 Size: 171 465 4852 Fragments arranged by size: 4852 465 171 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FspI TGC'GCA Cuts at: 2559 3994 2559 Size: 1435 4053 HaeII r_GCGC'y Cuts at: 1678 4635 1678 Size: 2957 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4129 4161 4203 4235 1373 Size: 32 135 32 2557 32 42 32 2626 Fragments arranged by size: 2626 2557 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2780 10 Size: 54 1425 343 948 2718 Fragments arranged by size: 2718 1425 948 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 2774 3446 3805 3964 518 Size: 648 1572 36 672 359 159 2042 Fragments arranged by size: 2042 1572 672 648 359 159 36 MspA1I CmG'CkG Cuts at: 1606 2192 2904 3581 4729 4974 1606 Size: 586 712 677 1148 245 2120 Fragments arranged by size: 2120 1148 712 677 586 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4936 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2182 2011 Size: 171 5317 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PspOMI G'GGCC_C Cuts at: 2647 2647 Size: 5488 PstI C_TGCA'G Cuts at: 830 2840 830 Size: 2010 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 PvuII CAG'CTG Cuts at: 2192 2192 Size: 5488 SacI G_AGCT'C Cuts at: 2400 2400 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SanDI GG'GwC_CC Cuts at: 2011 2011 Size: 5488 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2836 3971 4652 4843 826 Size: 219 1791 1135 681 191 1471 Fragments arranged by size: 1791 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3592 4493 4755 820 Size: 197 926 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2722 1517 Size: 1205 4283 StyI C'CwwG_G Cuts at: 513 1065 2736 2927 513 Size: 552 1671 191 3074 Fragments arranged by size: 3074 1671 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2447 3515 3532 3685 3870 4315 325 Size: 1255 867 1068 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1068 867 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3734 5446 Size: 274 80 33 51 1443 753 1004 1712 Cuts at: 5446 96 Size: 138 Fragments arranged by size: 1712 1443 1004 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2049 2081 5448 5480 2049 Size: 32 3367 32 2057 Fragments arranged by size: 3367 2057 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 2330 3617 2330 Size: 1287 4201 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI