################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcyI 661 GGCGTC G[GA]^CG[CT]C AhdI 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 955 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 992 CCTCAGG CC^T[ACGT]AGG Aor51HI 432 AGCGCT AGC^GCT ApaI 715 GGGCCC GGGCC^C AspEI 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 807 GGATAC GTATCC(6/5) BsaAI 415 TACGTG [CT]AC^GT[GA] BsaHI 661 GGCGTC G[GA]^CG[CT]C BsrBI 835 GAGCGG CCGCTC(-3/-3) BsrDI 173 GCAATG GCAATG(2/0) BstEII 879 GGTGACC G^GT[ACGT]ACC BstPI 879 GGTGACC G^GT[ACGT]ACC Bsu36I 992 CCTCAGG CC^T[ACGT]AGG DpnII 817 GATC ^GATC EagI 465 CGGCCG C^GGCCG Eam1105I 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 465 CGGCCG C^GGCCG Eco47III 432 AGCGCT AGC^GCT Eco52I 465 CGGCCG C^GGCCG Eco81I 992 CCTCAGG CC^T[ACGT]AGG EcoO65I 879 GGTGACC G^GT[ACGT]ACC Hin1I 661 GGCGTC G[GA]^CG[CT]C MaeII 416 ACGT A^CGT MboI 817 GATC ^GATC NdeII 817 GATC ^GATC NlaIII 666 CATG CATG^ PflFI 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 956 AGGACCT [GA]G^G[AT]CC[CT] PstI 775 CTGCAG CTGCA^G Sau3AI 817 GATC ^GATC SgrAI 724 CGCCGGCG C[GA]^CCGG[CT]G StuI 110 AGGCCT AGG^CCT Tth111I 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AvaI 586 CCCGAG C^[CT]CG[GA]G 718 CCCGAG AvaII 183 GGTCC G^G[AT]CC 957 GGACC BalI 188 TGGCCA TGG^CCA 419 TGGCCA BanII 302 GGGCTC G[GA]GC[CT]^C 715 GGGCCC BcgI 214 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 860 GCAAGGATGTCG BlpI 70 GCTGAGC GC^T[ACGT]AGC 1046 GCTCAGC BmyI 302 GGGCTC G[AGT]GC[ACT]^C 715 GGGCCC BpmI 244 CTCCAG CTGGAG(16/14) 315 CTGGAG Bpu1102I 70 GCTGAGC GC^T[ACGT]AGC 1046 GCTCAGC BsaI 1060 GAGACC GGTCTC(1/5) 480 GGTCTC BsiEI 408 CGACCG CG[GA][CT]^CG 465 CGGCCG BsmFI 4 GGGAC GGGAC(10/14) 1079 GGGAC BsoBI 586 CCCGAG C^[CT]CG[GA]G 718 CCCGAG Bsp1286I 302 GGGCTC G[AGT]GC[ACT]^C 715 GGGCCC BsrI 822 ACTGG ACTGG(1/-1) 134 CCAGT BssHII 217 GCGCGC G^CGCGC 829 GCGCGC CelII 70 GCTGAGC GC^T[ACGT]AGC 1046 GCTCAGC HinfI 279 GACTC G^A[ACGT]TC 318 GAGTC HphI 606 GGTGA GGTGA(8/7) 879 GGTGA KspI 448 CCGCGG CCGC^GG 1011 CCGCGG MaeIII 504 GTCAC ^GT[ACGT]AC 880 GTGAC MluNI 188 TGGCCA TGG^CCA 419 TGGCCA MscI 188 TGGCCA TGG^CCA 419 TGGCCA PleI 279 GACTC GAGTC(4/5) 318 GAGTC PvuII 247 CAGCTG CAG^CTG 778 CAGCTG SacII 448 CCGCGG CCGC^GG 1011 CCGCGG SapI 304 GCTCTTC GCTCTTC(1/4) 531 GCTCTTC SfcI 775 CTGCAG C^T[GA][CT]AG 786 CTACAG Tsp45I 504 GTCAC ^GT[GC]AC 880 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BsaJI BsiYI BslI BsmAI BspMI BsrFI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV Sau96I ScrFI TseI TspRI