Restriction map for pFN21AB8971
 (Circular) (Six-base) MAPSORT of: pFN21AB8971.seq  Check: 9273  from: 1  to: 5791



pFN21AB8971  5791 bp

 With 182 enzymes: SgfI * 

                        December 28, 2011 15:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2253    2348    2626    2253 
   Size:         95     278    5418

  Fragments arranged by size: 

                5418     278      95
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3601     278 
   Size:         53      83     186     936    2065    2468

  Fragments arranged by size: 

                2468    2065     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3134    1937 
   Size:       1197    4594
 
Acc65I G'GTAC_C

Cuts at:   3112    3112 
   Size:       5791
 
AclI AA'CG_TT

Cuts at:   1563    3090    3918    4291    1563 
   Size:       1527     828     373    3063

  Fragments arranged by size: 

                3063    1527     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2104    2297    2524    3854    5238    5506    1632 
   Size:        472     193     227    1330    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1330     472     268     227     193
 
AfeI AGC'GCT

Cuts at:   2439    2439 
   Size:       5791
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5594
 
AflIII A'CryG_T

Cuts at:   1203    4690    5667    1203 
   Size:       3487     977    1327

  Fragments arranged by size: 

                3487    1327     977
 
AgeI A'CCGG_T

Cuts at:   4622    4622 
   Size:       5791
 
AhdI GACnn_n'nnGTC

Cuts at:   3072    4520    3072 
   Size:       1448    4343
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5791
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5791
 
AlwNI CAG_nnn'CTG

Cuts at:   2965    3534    5106    2965 
   Size:        569    1572    3650

  Fragments arranged by size: 

                3650    1572     569
 
ApaI G_GGCC'C

Cuts at:   2724    2724 
   Size:       5791
 
ApaLI G'TGCA_C

Cuts at:   3848    5004    3848 
   Size:       1156    4635
 
ApoI r'AATT_y

Cuts at:   1493    3100    3326    3478    5587    1493 
   Size:       1607     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1607     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4345     160 
   Size:        872    3313    1606

  Fragments arranged by size: 

                3313    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5791
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2591    2723    3116    1870 
   Size:         73     648     132     393    4545

  Fragments arranged by size: 

                4545     648     393     132      73
 
BamHI G'GATC_C

Cuts at:   3121    3488    3121 
   Size:        367    5424
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3112    4567     618 
   Size:        325     217     627    1325    1455    1842

  Fragments arranged by size: 

                1842    1455    1325     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2311    2724    3110    1391 
   Size:        920     413     386    4072

  Fragments arranged by size: 

                4072     920     413     386
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4820
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2208    2242    2854    2888    3982    4016    1903 
   Size:         34     271      34     612      34    1094      34    3678

  Fragments arranged by size: 

                3678    1094     612     271      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   2806    3683    4893    2806 
   Size:        877    1210    3704

  Fragments arranged by size: 

                3704    1210     877
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5791
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4402     136 
   Size:        107     122      71     631     605    2730    1525

  Fragments arranged by size: 

                2730    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5786    5786 
   Size:       5791
 
BlpI GC'TnA_GC

Cuts at:   2076    3052    3208    2076 
   Size:        976     156    4659

  Fragments arranged by size: 

                4659     976     156
 
Bme1580I G_kGCm'C

Cuts at:   1790    2724    3852    5008    1790 
   Size:        934    1128    1156    2573

  Fragments arranged by size: 

                2573    1156    1128     934
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5791
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2133    3522    4480 
   Size:        416      53      83     399      24     716    1389     958

Cuts at:   4480     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1389     958     716     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5791
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2234    2341    4451    1239 
   Size:        995     107    2110    2579

  Fragments arranged by size: 

                2579    2110     995     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3916    4781    5079    3916 
   Size:        865     298    4628

  Fragments arranged by size: 

                4628     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2491    3059    4454     882 
   Size:        617     992     568    1395    2219

  Fragments arranged by size: 

                2219    1395     992     617     568
 
BsaAI yAC'GTr

Cuts at:    493    1204    2422    3582     493 
   Size:        711    1218    1160    2702

  Fragments arranged by size: 

                2702    1218    1160     711
 
BsaBI GATnn'nnATC

Cuts at:   5785    5785 
   Size:       5791
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2667    3598    3980    4615 
   Size:         53      83     186     936    1134     931     382     635

Cuts at:   4615     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1134     936     931     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4224    4622    4896    5043    5454    4224 
   Size:        398     274     147     411    4561

  Fragments arranged by size: 

                4561     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2354    2384    2471    2501    3062 
   Size:         30     165      30     951      30      87      30     561

Cuts at:   3062    3092    1178 
   Size:         30    3877

  Fragments arranged by size: 

                3877     951     561     165      87      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5791
 
BseYI C'CCAG_C

Cuts at:   2164    2443    2662    4994    5490    2164 
   Size:        279     219    2332     496    2465

  Fragments arranged by size: 

                2465    2332     496     279     219
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5218
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2416    2473    3241    4002    5030    1540 
   Size:        275     186     415      57     768     761    1028    2301

  Fragments arranged by size: 

                2301    1028     768     761     415     275     186      57
            
 
BsiHKAI G_wGCw'C

Cuts at:   3852    3937    5008    3852 
   Size:         85    1071    4635

  Fragments arranged by size: 

                4635    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3302    3395    3302 
   Size:         93    5698
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2311    2724    3110    3852    3937    5008    1391 
   Size:        399     521     413     386     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     521     413     399     386      85
            
 
BspEI T'CCGG_A

Cuts at:   5454    5454 
   Size:       5791
 
BspHI T'CATG_A

Cuts at:   3680    5410    3680 
   Size:       1730    4061
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2017    2253    2348    2626    2973    3146     844 
   Size:       1173     236      95     278     347     173    3489

  Fragments arranged by size: 

                3489    1173     347     278     236     173      95
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2842    3678    1394 
   Size:        429    1019     836    3507

  Fragments arranged by size: 

                3507    1019     836     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2185    4286    4460      65 
   Size:       1183     937    2101     174    1396

  Fragments arranged by size: 

                2101    1396    1183     937     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2174    2381    2730    3030    4435    4622    1700 
   Size:        110     364     207     349     300    1405     187    2869

  Fragments arranged by size: 

                2869    1405     364     349     300     207     187     110
            
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5791
 
BssHII G'CGCG_C

Cuts at:   1925    2222    2834    1925 
   Size:        297     612    4882

  Fragments arranged by size: 

                4882     612     297
 
BssSI C'ACGA_G

Cuts at:   3851    4863    3851 
   Size:       1012    4779
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5791
 
BstBI TT'CG_AA

Cuts at:   4595    4595 
   Size:       5791
 
BstEII G'GTnAC_C

Cuts at:   1184    2884    1184 
   Size:       1700    4091
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2048    2203    2896    2048 
   Size:        155     693    4943

  Fragments arranged by size: 

                4943     693     155
 
BstYI r'GATC_y

Cuts at:   1972    3121    3488    3873    3890    5342    5428    5440    5786 
   Size:       1149     367     385      17    1452      86      12     346

Cuts at:   5786    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1149     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2998    2998 
   Size:       5791
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2154    2453    2628    3016    3060     513 
   Size:        552     939     150     299     175     388      44    3244

  Fragments arranged by size: 

                3244     939     552     388     299     175     150      44
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2413     487 
   Size:        683      56    1187    3865

  Fragments arranged by size: 

                3865    1187     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2081    2510    2876    3307    4101    4121    2081 
   Size:        429     366     431     794      20    3751

  Fragments arranged by size: 

                3751     794     431     429     366      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5791
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5756
 
DraI TTT'AAA

Cuts at:   3095    3095 
   Size:       5791
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4798     809 
   Size:        507    3482    1802

  Fragments arranged by size: 

                3482    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2172    2193    2262 
   Size:         54    1425      50     275      18     342      21      69

Cuts at:   2262    2424    2470    2709    3238    4127    5756       8 
   Size:        162      46     239     529     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     529     342     275     239     162
                  69      54      50      46      43      21      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2470    3238    1537 
   Size:        275     658     768    4090

  Fragments arranged by size: 

                4090     768     658     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2052    2316    2396    2543    3721    1353 
   Size:        699     264      80     147    1178    3423

  Fragments arranged by size: 

                3423    1178     699     264     147      80
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2945    4378    4752    4898     387 
   Size:       1064     138    1356    1433     374     146    1280

  Fragments arranged by size: 

                1433    1356    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2104    2234    2297    2341    2524    3854    4451 
   Size:        393     472     130      63      44     183    1330     597

Cuts at:   4451    5238    5506    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524    1330     787     597     472     393     268     183
                 130      63      44
 
EcoO109I rG'GnC_Cy

Cuts at:   2596    2720    2962    3002    2596 
   Size:        124     242      40    5385

  Fragments arranged by size: 

                5385     242     124      40
 
EcoRI G'AATT_C

Cuts at:   3100    3478    3100 
   Size:        378    5413
 
FspI TGC'GCA

Cuts at:   4297    4297 
   Size:       5791
 
HaeII r_GCGC'y

Cuts at:   1678    2412    2441    2525    2628    2731    4938    1678 
   Size:        734      29      84     103     103    2207    2531

  Fragments arranged by size: 

                2531    2207     734     103     103      84      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2054    2086    3058    3090    4432 
   Size:         32     135      32     482      32     972      32    1342

Cuts at:   4432    4464    4506    4538    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1342     972     482     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3135    3381     669 
   Size:        988     281    1197     246    3079

  Fragments arranged by size: 

                3079    1197     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5791
 
HpaI GTT'AAC

Cuts at:   3381    3381 
   Size:       5791
 
KpnI G_GTAC'C

Cuts at:   3116    3116 
   Size:       5791
 
MfeI C'AATT_G

Cuts at:   3390    3390 
   Size:       5791
 
MluI A'CGCG_T

Cuts at:   5667    5667 
   Size:       5791
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2158    4905    5089    1305 
   Size:        853    2747     184    2007

  Fragments arranged by size: 

                2747    2007     853     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2195    2426      10 
   Size:         54    1425     343     363     231    3375

  Fragments arranged by size: 

                3375    1425     363     343     231      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3749    4108    4267     518 
   Size:        648    2583     359     159    2042

  Fragments arranged by size: 

                2583    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2254    2455    2557    2662    2785    2892    2910    3018 
   Size:        648     201     102     105     123     107      18     108

Cuts at:   3018    3207    3884    5032    5277    1606 
   Size:        189     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     648     245     201     189     123
                 108     107     105     102      18
 
NaeI GCC'GGC

Cuts at:   2176    2383    2732    3032    2176 
   Size:        207     349     300    4935

  Fragments arranged by size: 

                4935     349     300     207
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4300

  Fragments arranged by size: 

                4300     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5791
 
NgoMIV G'CCGG_C

Cuts at:   2174    2381    2730    3030    2174 
   Size:        207     349     300    4935

  Fragments arranged by size: 

                4935     349     300     207
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5791
 
NotI GC'GGCC_GC

Cuts at:   3238    3238 
   Size:       5791
 
NspI r_CATG'y

Cuts at:   1132    1229    3149    4694    1132 
   Size:         97    1920    1545    2229

  Fragments arranged by size: 

                2229    1920    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2644    1348 
   Size:       1296    4495
 
PciI A'CATG_T

Cuts at:   4690    4690 
   Size:       5791
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5791
 
PmeI GTTT'AAAC

Cuts at:   3095    3095 
   Size:       5791
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3860    3892    5373    5405    3860 
   Size:         32    1481      32    4246

  Fragments arranged by size: 

                4246    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2962    2962 
   Size:       5791
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5791
 
PsiI TTA'TAA

Cuts at:   3361    3361 
   Size:       5791
 
PspOMI G'GGCC_C

Cuts at:   2720    2720 
   Size:       5791
 
PstI C_TGCA'G

Cuts at:    830    2784    3143     830 
   Size:       1954     359    3478

  Fragments arranged by size: 

                3478    1954     359
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5791
 
PvuII CAG'CTG

Cuts at:   2254    2785    2254 
   Size:        531    5260
 
SacII CC_GC'GG

Cuts at:   2456    3019    2456 
   Size:        563    5228
 
SalI G'TCGA_C

Cuts at:   1936    3133    1936 
   Size:       1197    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2316    2543    2316 
   Size:        227    5564
 
SbfI CC_TGCA'GG

Cuts at:   3143    3143 
   Size:       5791
 
ScaI AGT'ACT

Cuts at:   4039    5751    4039 
   Size:       1712    4079
 
SfcI C'TryA_G

Cuts at:    826    1045    2780    2791    3139    4274    4955    5146     826 
   Size:        219    1735      11     348    1135     681     191    1471

  Fragments arranged by size: 

                1735    1471    1135     681     348     219     191      11
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5791
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2730    1700 
   Size:       1030    4761
 
SmaI CCC'GGG

Cuts at:   1872    3118    1872 
   Size:       1246    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3895    4796    5058     820 
   Size:        197     926    1952     901     262    1553

  Fragments arranged by size: 

                1952    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3582     493 
   Size:       3089    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5791
 
SphI G_CATG'C

Cuts at:   1132    3149    1132 
   Size:       2017    3774
 
SspI AAT'ATT

Cuts at:      5      52    3715       5 
   Size:         47    3663    2081

  Fragments arranged by size: 

                3663    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2117    1517 
   Size:        600    5191
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3230     513 
   Size:        552     939    1226    3074

  Fragments arranged by size: 

                3074    1226     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2213    3818    3835    3988    4173    4618     325 
   Size:       1255     633    1605      17     153     185     445    1498

  Fragments arranged by size: 

                1605    1498    1255     633     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4037    5749      96 
   Size:        274      80      33      51    1443    2060    1712     138

  Fragments arranged by size: 

                2060    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2471    2503    3062    3094    5751    5783    2471 
   Size:         32     559      32    2657      32    2479

  Fragments arranged by size: 

                2657    2479     559      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2574    1316 
   Size:       1258    4533
 
XbaI T'CTAG_A

Cuts at:   3127    3127 
   Size:       5791
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5791
 
XmaI C'CCGG_G

Cuts at:   1870    3116    1870 
   Size:       1246    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3920    3920 
   Size:       5791
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3599     276 
   Size:         53      83     186     936    2065    2468

  Fragments arranged by size: 

                2468    2065     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BplI
   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI    EcoNI    EcoRV
     FalI     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI
     PfoI     PmlI    PspXI     PsrI    RsrII     SacI    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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