(Circular) (Six-base) MAPSORT of: pFN21AB8971.seq Check: 9273 from: 1 to: 5791 pFN21AB8971 5791 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2253 2348 2626 2253 Size: 95 278 5418 Fragments arranged by size: 5418 278 95 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3090 3918 4291 1563 Size: 1527 828 373 3063 Fragments arranged by size: 3063 1527 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2104 2297 2524 3854 5238 5506 1632 Size: 472 193 227 1330 1384 268 1917 Fragments arranged by size: 1917 1384 1330 472 268 227 193 AfeI AGC'GCT Cuts at: 2439 2439 Size: 5791 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 3072 4520 3072 Size: 1448 4343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2965 3534 5106 2965 Size: 569 1572 3650 Fragments arranged by size: 3650 1572 569 ApaI G_GGCC'C Cuts at: 2724 2724 Size: 5791 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2591 2723 3116 1870 Size: 73 648 132 393 4545 Fragments arranged by size: 4545 648 393 132 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3112 4567 618 Size: 325 217 627 1325 1455 1842 Fragments arranged by size: 1842 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 2311 2724 3110 1391 Size: 920 413 386 4072 Fragments arranged by size: 4072 920 413 386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2208 2242 2854 2888 3982 4016 1903 Size: 34 271 34 612 34 1094 34 3678 Fragments arranged by size: 3678 1094 612 271 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2806 3683 4893 2806 Size: 877 1210 3704 Fragments arranged by size: 3704 1210 877 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 2076 3052 3208 2076 Size: 976 156 4659 Fragments arranged by size: 4659 976 156 Bme1580I G_kGCm'C Cuts at: 1790 2724 3852 5008 1790 Size: 934 1128 1156 2573 Fragments arranged by size: 2573 1156 1128 934 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2133 3522 4480 Size: 416 53 83 399 24 716 1389 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1389 958 716 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2341 4451 1239 Size: 995 107 2110 2579 Fragments arranged by size: 2579 2110 995 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 3059 4454 882 Size: 617 992 568 1395 2219 Fragments arranged by size: 2219 1395 992 617 568 BsaAI yAC'GTr Cuts at: 493 1204 2422 3582 493 Size: 711 1218 1160 2702 Fragments arranged by size: 2702 1218 1160 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2667 3598 3980 4615 Size: 53 83 186 936 1134 931 382 635 Cuts at: 4615 275 Size: 1451 Fragments arranged by size: 1451 1134 936 931 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 2471 2501 3062 Size: 30 165 30 951 30 87 30 561 Cuts at: 3062 3092 1178 Size: 30 3877 Fragments arranged by size: 3877 951 561 165 87 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5791 BseYI C'CCAG_C Cuts at: 2164 2443 2662 4994 5490 2164 Size: 279 219 2332 496 2465 Fragments arranged by size: 2465 2332 496 279 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2416 2473 3241 4002 5030 1540 Size: 275 186 415 57 768 761 1028 2301 Fragments arranged by size: 2301 1028 768 761 415 275 186 57 BsiHKAI G_wGCw'C Cuts at: 3852 3937 5008 3852 Size: 85 1071 4635 Fragments arranged by size: 4635 1071 85 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2311 2724 3110 3852 3937 5008 1391 Size: 399 521 413 386 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 521 413 399 386 85 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 2253 2348 2626 2973 3146 844 Size: 1173 236 95 278 347 173 3489 Fragments arranged by size: 3489 1173 347 278 236 173 95 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3678 1394 Size: 429 1019 836 3507 Fragments arranged by size: 3507 1019 836 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 4286 4460 65 Size: 1183 937 2101 174 1396 Fragments arranged by size: 2101 1396 1183 937 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2174 2381 2730 3030 4435 4622 1700 Size: 110 364 207 349 300 1405 187 2869 Fragments arranged by size: 2869 1405 364 349 300 207 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 2222 2834 1925 Size: 297 612 4882 Fragments arranged by size: 4882 612 297 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 2884 1184 Size: 1700 4091 BstXI CCAn_nnnn'nTGG Cuts at: 2048 2203 2896 2048 Size: 155 693 4943 Fragments arranged by size: 4943 693 155 BstYI r'GATC_y Cuts at: 1972 3121 3488 3873 3890 5342 5428 5440 5786 Size: 1149 367 385 17 1452 86 12 346 Cuts at: 5786 1972 Size: 1977 Fragments arranged by size: 1977 1452 1149 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2998 2998 Size: 5791 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2453 2628 3016 3060 513 Size: 552 939 150 299 175 388 44 3244 Fragments arranged by size: 3244 939 552 388 299 175 150 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2413 487 Size: 683 56 1187 3865 Fragments arranged by size: 3865 1187 683 56 BtsI GCAGTG_nn' Cuts at: 2081 2510 2876 3307 4101 4121 2081 Size: 429 366 431 794 20 3751 Fragments arranged by size: 3751 794 431 429 366 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2193 2262 Size: 54 1425 50 275 18 342 21 69 Cuts at: 2262 2424 2470 2709 3238 4127 5756 8 Size: 162 46 239 529 889 1629 43 Fragments arranged by size: 1629 1425 889 529 342 275 239 162 69 54 50 46 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2470 3238 1537 Size: 275 658 768 4090 Fragments arranged by size: 4090 768 658 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2316 2396 2543 3721 1353 Size: 699 264 80 147 1178 3423 Fragments arranged by size: 3423 1178 699 264 147 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2945 4378 4752 4898 387 Size: 1064 138 1356 1433 374 146 1280 Fragments arranged by size: 1433 1356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2234 2297 2341 2524 3854 4451 Size: 393 472 130 63 44 183 1330 597 Cuts at: 4451 5238 5506 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1330 787 597 472 393 268 183 130 63 44 EcoO109I rG'GnC_Cy Cuts at: 2596 2720 2962 3002 2596 Size: 124 242 40 5385 Fragments arranged by size: 5385 242 124 40 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2412 2441 2525 2628 2731 4938 1678 Size: 734 29 84 103 103 2207 2531 Fragments arranged by size: 2531 2207 734 103 103 84 29 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 3058 3090 4432 Size: 32 135 32 482 32 972 32 1342 Cuts at: 4432 4464 4506 4538 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1342 972 482 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2158 4905 5089 1305 Size: 853 2747 184 2007 Fragments arranged by size: 2747 2007 853 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2426 10 Size: 54 1425 343 363 231 3375 Fragments arranged by size: 3375 1425 363 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 3749 4108 4267 518 Size: 648 2583 359 159 2042 Fragments arranged by size: 2583 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2455 2557 2662 2785 2892 2910 3018 Size: 648 201 102 105 123 107 18 108 Cuts at: 3018 3207 3884 5032 5277 1606 Size: 189 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 245 201 189 123 108 107 105 102 18 NaeI GCC'GGC Cuts at: 2176 2383 2732 3032 2176 Size: 207 349 300 4935 Fragments arranged by size: 4935 349 300 207 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4300 Fragments arranged by size: 4300 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NgoMIV G'CCGG_C Cuts at: 2174 2381 2730 3030 2174 Size: 207 349 300 4935 Fragments arranged by size: 4935 349 300 207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 3149 4694 1132 Size: 97 1920 1545 2229 Fragments arranged by size: 2229 1920 1545 97 PasI CC'CwG_GG Cuts at: 1348 2644 1348 Size: 1296 4495 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2962 2962 Size: 5791 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PspOMI G'GGCC_C Cuts at: 2720 2720 Size: 5791 PstI C_TGCA'G Cuts at: 830 2784 3143 830 Size: 1954 359 3478 Fragments arranged by size: 3478 1954 359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2254 2785 2254 Size: 531 5260 SacII CC_GC'GG Cuts at: 2456 3019 2456 Size: 563 5228 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SapI GCTCTTCn'nnn_ Cuts at: 2316 2543 2316 Size: 227 5564 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 2780 2791 3139 4274 4955 5146 826 Size: 219 1735 11 348 1135 681 191 1471 Fragments arranged by size: 1735 1471 1135 681 348 219 191 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 2730 1700 Size: 1030 4761 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3895 4796 5058 820 Size: 197 926 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2117 1517 Size: 600 5191 StyI C'CwwG_G Cuts at: 513 1065 2004 3230 513 Size: 552 939 1226 3074 Fragments arranged by size: 3074 1226 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3818 3835 3988 4173 4618 325 Size: 1255 633 1605 17 153 185 445 1498 Fragments arranged by size: 1605 1498 1255 633 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2471 2503 3062 3094 5751 5783 2471 Size: 32 559 32 2657 32 2479 Fragments arranged by size: 2657 2479 559 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2574 1316 Size: 1258 4533 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI