Restriction map for
pFN21AB7330
(Linear) (Six-base) MAPSORT of: KIBB7330.seq Check: 2539 from: 1 to: 2616
KIBB7330.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 0 1700 2616
Size: 1700 916
AccI GT'mk_AC
Cuts at: 0 29 2039 2616
Size: 29 2010 577
Fragments arranged by size:
2010 577 29
Acc65I G'GTAC_C
Cuts at: 0 458 2616
Size: 458 2158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 973 2388 2397 2616
Size: 79 894 1415 9 219
Fragments arranged by size:
1415 894 219 79 9
AflIII A'CryG_T
Cuts at: 0 293 2354 2616
Size: 293 2061 262
Fragments arranged by size:
2061 293 262
AhdI GACnn_n'nnGTC
Cuts at: 0 1734 2616
Size: 1734 882
AleI CACnn'nnGTG
Cuts at: 0 804 2616
Size: 804 1812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 465 1081 2616
Size: 465 616 1535
Fragments arranged by size:
1535 616 465
AlwNI CAG_nnn'CTG
Cuts at: 0 615 2616
Size: 615 2001
ApaI G_GGCC'C
Cuts at: 0 375 1271 1789 1822 2552 2609 2616
Size: 375 896 518 33 730 57 7
Fragments arranged by size:
896 730 518 375 57 33 7
ApoI r'AATT_y
Cuts at: 0 234 2616
Size: 234 2382
AscI GG'CGCG_CC
Cuts at: 0 89 2616
Size: 89 2527
AvaI C'yCGr_G
Cuts at: 0 39 104 1340 1417 1599 1840 1867 2313
Size: 39 65 1236 77 182 241 27 446
Cuts at: 2313 2329 2616
Size: 16 287
Fragments arranged by size:
1236 446 287 241 182 77 65 39
27 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2520 2553 2616
Size: 2520 33 63
Fragments arranged by size:
2520 63 33
BanI G'GyrC_C
Cuts at: 0 187 458 646 1437 1498 1998 2106 2185
Size: 187 271 188 791 61 500 108 79
Cuts at: 2185 2472 2616
Size: 287 144
Fragments arranged by size:
791 500 287 271 188 187 144 108
79 61
BanII G_rGCy'C
Cuts at: 0 364 375 667 1271 1789 1822 2552 2609
Size: 364 11 292 604 518 33 730 57
Cuts at: 2609 2616
Size: 7
Fragments arranged by size:
730 604 518 364 292 57 33 11
7
BbeI G_GCGC'C
Cuts at: 0 191 2002 2110 2476 2616
Size: 191 1811 108 366 140
Fragments arranged by size:
1811 366 191 140 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 1320 2027 2616
Size: 1320 707 589
Fragments arranged by size:
1320 707 589
BbvCI CC'TCA_GC
Cuts at: 0 160 1180 2616
Size: 160 1020 1436
Fragments arranged by size:
1436 1020 160
BclI T'GATC_A
Cuts at: 0 815 2616
Size: 815 1801
BglI GCCn_nnn'nGGC
Cuts at: 0 1343 2616
Size: 1343 1273
Bme1580I G_kGCm'C
Cuts at: 0 375 1109 1271 1442 1476 1789 1822 2552
Size: 375 734 162 171 34 313 33 730
Cuts at: 2552 2609 2616
Size: 57 7
Fragments arranged by size:
734 730 375 313 171 162 57 34
33 7
BmgBI CAC'GTC
Cuts at: 0 1689 2357 2592 2616
Size: 1689 668 235 24
Fragments arranged by size:
1689 668 235 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 2418 2616
Size: 2418 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 1728 2616
Size: 378 1350 888
Fragments arranged by size:
1350 888 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 489 1050 1128 1192 1317 1470 2058
Size: 274 215 561 78 64 125 153 588
Cuts at: 2058 2413 2616
Size: 355 203
Fragments arranged by size:
588 561 355 274 215 203 153 125
78 64
Bpu10I CC'TnA_GC
Cuts at: 0 160 1180 2616
Size: 160 1020 1436
Fragments arranged by size:
1436 1020 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1174 2616
Size: 466 708 1442
Fragments arranged by size:
1442 708 466
BsaI GGTCTCn'nnnn_
Cuts at: 0 1954 2616
Size: 1954 662
BsaAI yAC'GTr
Cuts at: 0 294 1076 2616
Size: 294 782 1540
Fragments arranged by size:
1540 782 294
BsaBI GATnn'nnATC
Cuts at: 0 514 1945 2616
Size: 514 1431 671
Fragments arranged by size:
1431 671 514
BsaHI Gr'CG_yC
Cuts at: 0 188 1697 1999 2107 2473 2616
Size: 188 1509 302 108 366 143
Fragments arranged by size:
1509 366 302 188 143 108
BsaWI w'CCGG_w
Cuts at: 0 590 2616
Size: 590 2026
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1054 1084 1126 1156 2173 2194 2203 2224
Size: 1054 30 42 30 1017 21 9 21
Cuts at: 2224 2497 2509 2527 2539 2616
Size: 273 12 18 12 77
Fragments arranged by size:
1054 1017 273 77 42 30 30 21
21 18 12 12 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2401 2616
Size: 2401 215
BseYI C'CCAG_C
Cuts at: 0 269 660 1399 1429 1788 1849 2597 2616
Size: 269 391 739 30 359 61 748 19
Fragments arranged by size:
748 739 391 359 269 61 30 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1051 1281 2616
Size: 25 1026 230 1335
Fragments arranged by size:
1335 1026 230 25
BsiEI CG_ry'CG
Cuts at: 0 262 1826 2117 2251 2468 2616
Size: 262 1564 291 134 217 148
Fragments arranged by size:
1564 291 262 217 148 134
BsiHKAI G_wGCw'C
Cuts at: 0 364 527 2616
Size: 364 163 2089
Fragments arranged by size:
2089 364 163
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1244 2616
Size: 1244 1372
Bsp1286I G_dGCh'C
Cuts at: 0 364 375 527 667 1109 1271 1442 1476
Size: 364 11 152 140 442 162 171 34
Cuts at: 1476 1789 1822 2552 2609 2616
Size: 313 33 730 57 7
Fragments arranged by size:
730 442 364 313 171 162 152 140
57 34 33 11 7
BspEI T'CCGG_A
Cuts at: 0 590 2616
Size: 590 2026
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1073 1875 2348 2616
Size: 1073 802 473 268
Fragments arranged by size:
1073 802 473 268
BsrBI CCG'CTC
Cuts at: 0 44 258 2247 2616
Size: 44 214 1989 369
Fragments arranged by size:
1989 369 214 44
BsrDI GCAATG_nn'
Cuts at: 0 536 2498 2616
Size: 536 1962 118
Fragments arranged by size:
1962 536 118
BsrFI r'CCGG_y
Cuts at: 0 127 455 553 635 1717 1995 2569 2616
Size: 127 328 98 82 1082 278 574 47
Fragments arranged by size:
1082 574 328 278 127 98 82 47
BsrGI T'GTAC_A
Cuts at: 0 1112 2616
Size: 1112 1504
BssHII G'CGCG_C
Cuts at: 0 89 2616
Size: 89 2527
BssSI C'ACGA_G
Cuts at: 0 319 2242 2383 2616
Size: 319 1923 141 233
Fragments arranged by size:
1923 319 233 141
BstAPI GCAn_nnn'nTGC
Cuts at: 0 615 1259 2616
Size: 615 644 1357
Fragments arranged by size:
1357 644 615
BstEII G'GTnAC_C
Cuts at: 0 1098 1394 2616
Size: 1098 296 1222
Fragments arranged by size:
1222 1098 296
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1857 2616
Size: 1857 759
BstYI r'GATC_y
Cuts at: 0 431 2616
Size: 431 2185
BstZ17I GTA'TAC
Cuts at: 0 30 2616
Size: 30 2586
BtgI C'CryG_G
Cuts at: 0 462 1895 2084 2181 2195 2205 2375 2468
Size: 462 1433 189 97 14 10 170 93
Cuts at: 2468 2616
Size: 148
Fragments arranged by size:
1433 462 189 170 148 97 93 14
10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 513 2616
Size: 513 2103
BtsI GCAGTG_nn'
Cuts at: 0 698 2616
Size: 698 1918
EaeI y'GGCC_r
Cuts at: 0 259 548 1719 1823 2114 2465 2564 2616
Size: 259 289 1171 104 291 351 99 52
Fragments arranged by size:
1171 351 291 289 259 104 99 52
EagI C'GGCC_G
Cuts at: 0 259 1823 2114 2465 2616
Size: 259 1564 291 351 151
Fragments arranged by size:
1564 351 291 259 151
EarI CTCTTCn'nnn_
Cuts at: 0 352 985 2616
Size: 352 633 1631
Fragments arranged by size:
1631 633 352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1011 1846 2126 2247 2616
Size: 1011 835 280 121 369
Fragments arranged by size:
1011 835 369 280 121
EcoICRI GAG'CTC
Cuts at: 0 362 2616
Size: 362 2254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 274 489 973 1050 1128 1192 1317
Size: 79 195 215 484 77 78 64 125
Cuts at: 1317 1470 2058 2388 2397 2413 2616
Size: 153 588 330 9 16 203
Fragments arranged by size:
588 484 330 215 203 195 153 125
79 78 77 64 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2299 2616
Size: 2299 317
EcoO109I rG'GnC_Cy
Cuts at: 0 138 371 372 810 1139 1221 1267 1268
Size: 138 233 1 438 329 82 46 1
Cuts at: 1268 1367 1590 1785 1818 1845 2324 2492 2549
Size: 99 223 195 33 27 479 168 57
Cuts at: 2549 2578 2616
Size: 29 38
Fragments arranged by size:
479 438 329 233 223 195 168 138
99 82 57 46 38 33 29 27
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 84 1905 1929 2616
Size: 84 1821 24 687
Fragments arranged by size:
1821 687 84 24
FspI TGC'GCA
Cuts at: 0 2221 2616
Size: 2221 395
FspAI rTGC'GCAy
Cuts at: 0 2221 2616
Size: 2221 395
HaeII r_GCGC'y
Cuts at: 0 118 191 1406 2002 2110 2476 2616
Size: 118 73 1215 596 108 366 140
Fragments arranged by size:
1215 596 366 140 118 108 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1720 1752 2194 2226 2497 2509
Size: 1054 32 634 32 442 32 271 12
Cuts at: 2509 2529 2541 2616
Size: 20 12 75
Fragments arranged by size:
1054 634 442 271 75 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 2040 2616
Size: 2040 576
KasI G'GCGC_C
Cuts at: 0 187 1998 2106 2472 2616
Size: 187 1811 108 366 144
Fragments arranged by size:
1811 366 187 144 108
KpnI G_GTAC'C
Cuts at: 0 462 2616
Size: 462 2154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2552 2616
Size: 2552 64
MslI CAynn'nnrTG
Cuts at: 0 520 541 804 2616
Size: 520 21 263 1812
Fragments arranged by size:
1812 520 263 21
MspA1I CmG'CkG
Cuts at: 0 1161 1170 1938 2408 2456 2616
Size: 1161 9 768 470 48 160
Fragments arranged by size:
1161 768 470 160 48 9
NaeI GCC'GGC
Cuts at: 0 129 555 1719 1997 2616
Size: 129 426 1164 278 619
Fragments arranged by size:
1164 619 426 278 129
NarI GG'CG_CC
Cuts at: 0 188 1999 2107 2473 2616
Size: 188 1811 108 366 143
Fragments arranged by size:
1811 366 188 143 108
NcoI C'CATG_G
Cuts at: 0 1895 2616
Size: 1895 721
NgoMIV G'CCGG_C
Cuts at: 0 127 553 1717 1995 2616
Size: 127 426 1164 278 621
Fragments arranged by size:
1164 621 426 278 127
NotI GC'GGCC_GC
Cuts at: 0 259 2616
Size: 259 2357
NspI r_CATG'y
Cuts at: 0 1734 1935 2038 2616
Size: 1734 201 103 578
Fragments arranged by size:
1734 578 201 103
PasI CC'CwG_GG
Cuts at: 0 561 2616
Size: 561 2055
PflMI CCAn_nnn'nTGG
Cuts at: 0 1448 1776 2150 2616
Size: 1448 328 374 466
Fragments arranged by size:
1448 466 374 328
PfoI T'CCnGG_A
Cuts at: 0 383 2616
Size: 383 2233
PmlI CAC'GTG
Cuts at: 0 1076 2616
Size: 1076 1540
PpuMI rG'GwC_Cy
Cuts at: 0 1221 1367 1590 1845 2578 2616
Size: 1221 146 223 255 733 38
Fragments arranged by size:
1221 733 255 223 146 38
PspOMI G'GGCC_C
Cuts at: 0 371 1267 1785 1818 2548 2605 2616
Size: 371 896 518 33 730 57 11
Fragments arranged by size:
896 730 518 371 57 33 11
PspXI vC'TCGA_Gb
Cuts at: 0 1417 2616
Size: 1417 1199
PstI C_TGCA'G
Cuts at: 0 233 611 927 1757 2323 2616
Size: 233 378 316 830 566 293
Fragments arranged by size:
830 566 378 316 293 233
PvuII CAG'CTG
Cuts at: 0 1170 2456 2616
Size: 1170 1286 160
Fragments arranged by size:
1286 1170 160
SacI G_AGCT'C
Cuts at: 0 364 2616
Size: 364 2252
SalI G'TCGA_C
Cuts at: 0 2038 2616
Size: 2038 578
SanDI GG'GwC_CC
Cuts at: 0 1367 2578 2616
Size: 1367 1211 38
Fragments arranged by size:
1367 1211 38
SapI GCTCTTCn'nnn_
Cuts at: 0 352 2616
Size: 352 2264
SbfI CC_TGCA'GG
Cuts at: 0 927 2616
Size: 927 1689
ScaI AGT'ACT
Cuts at: 0 421 1084 1711 2616
Size: 421 663 627 905
Fragments arranged by size:
905 663 627 421
SfcI C'TryA_G
Cuts at: 0 229 607 923 1753 2319 2616
Size: 229 378 316 830 566 297
Fragments arranged by size:
830 566 378 316 297 229
SfoI GGC'GCC
Cuts at: 0 189 2000 2108 2474 2616
Size: 189 1811 108 366 142
Fragments arranged by size:
1811 366 189 142 108
SgrAI Cr'CCGG_yG
Cuts at: 0 2569 2616
Size: 2569 47
SmaI CCC'GGG
Cuts at: 0 106 1601 2315 2616
Size: 106 1495 714 301
Fragments arranged by size:
1495 714 301 106
SmlI C'TyrA_G
Cuts at: 0 481 1189 1417 2616
Size: 481 708 228 1199
Fragments arranged by size:
1199 708 481 228
StuI AGG'CCT
Cuts at: 0 1013 1969 2616
Size: 1013 956 647
Fragments arranged by size:
1013 956 647
StyI C'CwwG_G
Cuts at: 0 1355 1814 1895 2429 2616
Size: 1355 459 81 534 187
Fragments arranged by size:
1355 534 459 187 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1413 1600 1978 2616
Size: 1413 187 378 638
Fragments arranged by size:
1413 638 378 187
TatI w'GTAC_w
Cuts at: 0 419 749 1082 1112 1709 2616
Size: 419 330 333 30 597 907
Fragments arranged by size:
907 597 419 333 330 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2173 2205 2616
Size: 2173 32 411
Fragments arranged by size:
2173 411 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 543 2373 2436 2616
Size: 543 1830 63 180
Fragments arranged by size:
1830 543 180 63
XhoI C'TCGA_G
Cuts at: 0 1417 2616
Size: 1417 1199
XmaI C'CCGG_G
Cuts at: 0 104 1599 2313 2616
Size: 104 1495 714 303
Fragments arranged by size:
1495 714 303 104
XmnI GAAnn'nnTTC
Cuts at: 0 499 2098 2616
Size: 499 1599 518
Fragments arranged by size:
1599 518 499
ZraI GAC'GTC
Cuts at: 0 1698 2616
Size: 1698 918
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AscI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgrAI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AloI ApaLI AseI
AsiSI AvrII BamHI BcgI BciVI BfrBI BglII BlpI
BmtI BsiWI BsmI BspHI BstBI Bsu36I ClaI CspCI
DraI DraIII DrdI EcoRI EcoRV FseI HindIII HpaI
MfeI MluI MscI NdeI NheI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SexAI SfiI SgfI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI