(Circular) (Six-base) MAPSORT of: pFN21AB9879.seq Check: 734 from: 1 to: 6457 pFN21AB9879 6457 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3800 1937 Size: 1863 4594 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2071 2840 3302 4520 5904 6172 1632 Size: 439 769 462 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 769 462 439 268 AflII C'TTAA_G Cuts at: 820 1017 2219 820 Size: 197 1202 5058 Fragments arranged by size: 5058 1202 197 AflIII A'CryG_T Cuts at: 1203 5356 6333 1203 Size: 4153 977 1327 Fragments arranged by size: 4153 1327 977 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2159 2198 2631 1184 Size: 975 39 433 5010 Fragments arranged by size: 5010 975 433 39 AlwNI CAG_nnn'CTG Cuts at: 2519 2704 4200 5772 2519 Size: 185 1496 1572 3204 Fragments arranged by size: 3204 1572 1496 185 ApaI G_GGCC'C Cuts at: 2211 2268 3502 3623 2211 Size: 57 1234 121 5045 Fragments arranged by size: 5045 1234 121 57 ApaLI G'TGCA_C Cuts at: 4514 5670 4514 Size: 1156 5301 ApoI r'AATT_y Cuts at: 1493 3766 3992 4144 6253 1493 Size: 2273 226 152 2109 1697 Fragments arranged by size: 2273 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 2189 2204 3158 3597 3643 3782 1870 Size: 73 246 15 954 439 46 139 4545 Fragments arranged by size: 4545 954 439 246 139 73 46 15 BamHI G'GATC_C Cuts at: 3787 4154 3787 Size: 367 6090 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2548 2676 3127 3223 3356 Size: 325 217 627 761 128 451 96 133 Cuts at: 3356 3565 3778 5233 618 Size: 209 213 1455 1842 Fragments arranged by size: 1842 1455 761 627 451 325 217 213 209 133 128 96 BanII G_rGCy'C Cuts at: 1391 2211 2268 3112 3502 3623 3776 1391 Size: 820 57 844 390 121 153 4072 Fragments arranged by size: 4072 844 820 390 153 121 57 BbeI G_GCGC'C Cuts at: 2680 3131 3360 2680 Size: 451 229 5777 Fragments arranged by size: 5777 451 229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3543 928 Size: 971 1644 3842 Fragments arranged by size: 3842 1644 971 BbvCI CC'TCA_GC Cuts at: 2445 2445 Size: 6457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2771 2805 4648 4682 1903 Size: 34 834 34 1843 34 3678 Fragments arranged by size: 3678 1843 834 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2798 4349 5559 2798 Size: 1551 1210 3696 Fragments arranged by size: 3696 1551 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2497 3083 5068 Size: 107 122 71 631 605 825 586 1985 Cuts at: 5068 136 Size: 1525 Fragments arranged by size: 1985 1525 825 631 605 586 122 107 71 BglII A'GATC_T Cuts at: 6452 6452 Size: 6457 BlpI GC'TnA_GC Cuts at: 3874 3874 Size: 6457 Bme1580I G_kGCm'C Cuts at: 1790 2211 2268 3264 3502 3560 3570 3623 3662 Size: 421 57 996 238 58 10 53 39 Cuts at: 3662 3684 4518 5674 1790 Size: 22 834 1156 2573 Fragments arranged by size: 2573 1156 996 834 421 238 58 57 53 39 22 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3226 3492 4188 Size: 416 53 83 399 24 1809 266 696 Cuts at: 4188 5146 442 Size: 958 1753 Fragments arranged by size: 1809 1753 958 696 416 399 266 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2784 3744 5117 1239 Size: 1169 376 960 1373 2579 Fragments arranged by size: 2579 1373 1169 960 376 Bpu10I CC'TnA_GC Cuts at: 2445 2445 Size: 6457 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 2578 4582 5447 5745 2153 Size: 425 2004 865 298 2865 Fragments arranged by size: 2865 2004 865 425 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3242 5120 882 Size: 617 1743 1878 2219 Fragments arranged by size: 2219 1878 1743 617 BsaAI yAC'GTr Cuts at: 493 1204 2182 4248 493 Size: 711 978 2066 2702 Fragments arranged by size: 2702 2066 978 711 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2021 2677 2868 Size: 53 83 186 936 476 12 656 191 Cuts at: 2868 3015 3128 3357 4264 4646 5281 275 Size: 147 113 229 907 382 635 1451 Fragments arranged by size: 1451 936 907 656 635 476 382 229 191 186 147 113 83 53 12 BsaWI w'CCGG_w Cuts at: 4890 5288 5562 5709 6120 4890 Size: 398 274 147 411 5227 Fragments arranged by size: 5227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6232 Fragments arranged by size: 6232 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 3212 3364 3664 1186 Size: 1261 765 152 300 3979 Fragments arranged by size: 3979 1261 765 300 152 BseYI C'CCAG_C Cuts at: 2268 2289 2295 2422 2992 3057 3179 3384 3396 Size: 21 6 127 570 65 122 205 12 Cuts at: 3396 5660 6156 2268 Size: 2264 496 2569 Fragments arranged by size: 2569 2264 570 496 205 127 122 65 21 12 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 3369 3541 1163 Size: 573 300 1333 172 4079 Fragments arranged by size: 4079 1333 573 300 172 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3627 3907 4668 5696 1540 Size: 275 186 1626 280 761 1028 2301 Fragments arranged by size: 2301 1626 1028 761 280 275 186 BsiHKAI G_wGCw'C Cuts at: 2187 4518 4603 5674 2187 Size: 2331 85 1071 2970 Fragments arranged by size: 2970 2331 1071 85 BsmI GAATG_Cn' Cuts at: 2620 3968 4061 2620 Size: 1348 93 5016 Fragments arranged by size: 5016 1348 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2211 2268 3112 3264 3502 3560 Size: 399 397 24 57 844 152 238 58 Cuts at: 3560 3570 3623 3662 3684 3776 4518 4603 5674 Size: 10 53 39 22 92 742 85 1071 Cuts at: 5674 1391 Size: 2174 Fragments arranged by size: 2174 1071 844 742 399 397 238 152 92 85 58 57 53 39 24 22 10 BspEI T'CCGG_A Cuts at: 6120 6120 Size: 6457 BspHI T'CATG_A Cuts at: 4346 6076 4346 Size: 1730 4727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 3812 844 Size: 1217 1751 3489 Fragments arranged by size: 3489 1751 1217 BsrBI CCG'CTC Cuts at: 1394 1823 2199 3529 3648 4344 1394 Size: 429 376 1330 119 696 3507 Fragments arranged by size: 3507 1330 696 429 376 119 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 3270 4952 5126 65 Size: 1183 1612 410 1682 174 1396 Fragments arranged by size: 1682 1612 1396 1183 410 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2309 2864 2964 3073 5101 5288 1700 Size: 110 499 555 100 109 2028 187 2869 Fragments arranged by size: 2869 2028 555 499 187 110 109 100 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6457 BstBI TT'CG_AA Cuts at: 5261 5261 Size: 6457 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6457 BstXI CCAn_nnnn'nTGG Cuts at: 2242 2422 2242 Size: 180 6277 BstYI r'GATC_y Cuts at: 1972 3787 4154 4539 4556 6008 6094 6106 6452 Size: 1815 367 385 17 1452 86 12 346 Cuts at: 6452 1972 Size: 1977 Fragments arranged by size: 1977 1815 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2066 2073 2439 3042 3378 513 Size: 552 939 62 7 366 603 336 3592 Fragments arranged by size: 3592 939 603 552 366 336 62 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3386 487 Size: 683 56 2160 3558 Fragments arranged by size: 3558 2160 683 56 BtsI GCAGTG_nn' Cuts at: 2520 2694 2883 3973 4767 4787 2520 Size: 174 189 1090 794 20 4190 Fragments arranged by size: 4190 1090 794 189 174 20 ClaI AT'CG_AT Cuts at: 1554 2151 1554 Size: 597 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3700 3735 529 Size: 35 3136 35 3251 Fragments arranged by size: 3251 3136 35 35 DraI TTT'AAA Cuts at: 3761 3761 Size: 6457 DraIII CAC_nnn'GTG Cuts at: 3220 3220 Size: 6457 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5464 809 Size: 507 4148 1802 Fragments arranged by size: 4148 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2412 3291 Size: 54 1425 50 275 18 399 183 879 Cuts at: 3291 3624 3904 4793 6422 8 Size: 333 280 889 1629 43 Fragments arranged by size: 1629 1425 889 879 399 333 280 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3624 3904 1537 Size: 275 1812 280 4090 Fragments arranged by size: 4090 1812 280 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 2831 4387 1353 Size: 1229 249 1556 3423 Fragments arranged by size: 3423 1556 1229 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2704 3342 5044 5418 5564 387 Size: 1064 138 1115 638 1702 374 146 1280 Fragments arranged by size: 1702 1280 1115 1064 638 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2408 2784 2840 3302 3744 4520 Size: 393 439 337 376 56 462 442 776 Cuts at: 4520 5117 5904 6172 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 776 597 462 442 439 393 376 337 268 56 EcoNI CCTnn'n_nnAGG Cuts at: 3327 3327 Size: 6457 EcoO109I rG'GnC_Cy Cuts at: 2208 2239 2264 2265 2403 2460 3049 3319 3338 Size: 31 25 1 138 57 589 270 19 Cuts at: 3338 3498 3499 3619 3751 2208 Size: 160 1 120 132 4914 Fragments arranged by size: 4914 589 270 160 138 132 120 57 31 25 19 1 1 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3601 3601 Size: 6457 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 2680 2711 3131 3360 5604 1678 Size: 1002 31 420 229 2244 2531 Fragments arranged by size: 2531 2244 1002 420 229 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5098 5130 5172 5204 1373 Size: 32 135 32 3526 32 42 32 2626 Fragments arranged by size: 3526 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3801 4047 669 Size: 988 281 1863 246 3079 Fragments arranged by size: 3079 1863 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6457 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KasI G'GCGC_C Cuts at: 2676 3127 3356 2676 Size: 451 229 5777 Fragments arranged by size: 5777 451 229 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 4056 4056 Size: 6457 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5571 5755 1305 Size: 4266 184 2007 Fragments arranged by size: 4266 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 10 Size: 54 1425 343 582 4053 Fragments arranged by size: 4053 1425 582 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4415 4774 4933 518 Size: 648 3249 359 159 2042 Fragments arranged by size: 3249 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 2510 2992 3044 3245 3446 3465 3477 Size: 666 238 482 52 201 201 19 12 Cuts at: 3477 3664 3873 4550 5698 5943 1606 Size: 187 209 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 666 482 245 238 209 201 201 187 52 19 12 NaeI GCC'GGC Cuts at: 2866 2966 3075 2866 Size: 100 109 6248 Fragments arranged by size: 6248 109 100 NarI GG'CG_CC Cuts at: 2677 3128 3357 2677 Size: 451 229 5777 Fragments arranged by size: 5777 451 229 NcoI C'CATG_G Cuts at: 513 1065 2004 2073 513 Size: 552 939 69 4897 Fragments arranged by size: 4897 939 552 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NgoMIV G'CCGG_C Cuts at: 2864 2964 3073 2864 Size: 100 109 6248 Fragments arranged by size: 6248 109 100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 3815 5360 1132 Size: 97 2586 1545 2229 Fragments arranged by size: 2586 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2495 3301 3494 3684 1348 Size: 1147 806 193 190 4121 Fragments arranged by size: 4121 1147 806 193 190 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 2079 1108 Size: 971 5486 PfoI T'CCnGG_A Cuts at: 2800 3116 2800 Size: 316 6141 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PmlI CAC'GTG Cuts at: 2182 2182 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 6039 6071 4526 Size: 32 1481 32 4912 Fragments arranged by size: 4912 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2239 2403 3319 3751 2239 Size: 164 916 432 4945 Fragments arranged by size: 4945 916 432 164 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6457 PsiI TTA'TAA Cuts at: 4027 4027 Size: 6457 PspOMI G'GGCC_C Cuts at: 2207 2264 3498 3619 2207 Size: 57 1234 121 5045 Fragments arranged by size: 5045 1234 121 57 PstI C_TGCA'G Cuts at: 830 2515 3370 3809 830 Size: 1685 855 439 3478 Fragments arranged by size: 3478 1685 855 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 PvuII CAG'CTG Cuts at: 2272 2510 2992 2272 Size: 238 482 5737 Fragments arranged by size: 5737 482 238 RsrII CG'GwC_CG Cuts at: 2131 2131 Size: 6457 SacII CC_GC'GG Cuts at: 3045 3045 Size: 6457 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SanDI GG'GwC_CC Cuts at: 2403 3751 2403 Size: 1348 5109 SapI GCTCTTCn'nnn_ Cuts at: 2831 2831 Size: 6457 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 4705 6417 4705 Size: 1712 4745 SfcI C'TryA_G Cuts at: 826 1045 2511 3366 3805 4940 5621 5812 826 Size: 219 1466 855 439 1135 681 191 1471 Fragments arranged by size: 1471 1466 1135 855 681 439 219 191 SfoI GGC'GCC Cuts at: 2678 3129 3358 2678 Size: 451 229 5777 Fragments arranged by size: 5777 451 229 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 2864 1700 Size: 1164 5293 SmaI CCC'GGG Cuts at: 1872 2206 3599 3784 1872 Size: 334 1393 185 4545 Fragments arranged by size: 4545 1393 334 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2219 2557 4561 5462 5724 Size: 197 926 225 51 338 2004 901 262 Cuts at: 5724 820 Size: 1553 Fragments arranged by size: 2004 1553 926 901 338 262 225 197 51 SnaBI TAC'GTA Cuts at: 493 4248 493 Size: 3755 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SrfI GCCC'GGGC Cuts at: 2206 2206 Size: 6457 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 2538 1517 Size: 1021 5436 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2235 3315 3896 513 Size: 552 939 69 162 1080 581 3074 Fragments arranged by size: 3074 1080 939 581 552 162 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3208 3328 3557 4484 4501 4654 4839 Size: 1255 1628 120 229 927 17 153 185 Cuts at: 4839 5284 325 Size: 445 1498 Fragments arranged by size: 1628 1498 1255 927 445 229 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3636 4703 Size: 274 80 33 51 1443 414 1245 1067 Cuts at: 4703 6415 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1245 1067 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6417 6449 6417 Size: 32 6425 Tth111I GACn'n_nGTC Cuts at: 1316 3014 1316 Size: 1698 4759 XbaI T'CTAG_A Cuts at: 3793 3793 Size: 6457 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2239 Size: 6457 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6457 XmaI C'CCGG_G Cuts at: 1870 2204 3597 3782 1870 Size: 334 1393 185 4545 Fragments arranged by size: 4545 1393 334 185 XmnI GAAnn'nnTTC Cuts at: 2825 4586 2825 Size: 1761 4696 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI SacI SexAI SfiI SwaI