Restriction map for
pFN21AB8996
(Linear) (Six-base) MAPSORT of: KIBB8996.seq Check: 3740 from: 1 to: 1221
KIBB8996.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AleI CACnn'nnGTG
Cuts at: 0 1057 1221
Size: 1057 164
AlwNI CAG_nnn'CTG
Cuts at: 0 510 674 771 1221
Size: 510 164 97 450
Fragments arranged by size:
510 450 164 97
AvaI C'yCGr_G
Cuts at: 0 141 393 464 1221
Size: 141 252 71 757
Fragments arranged by size:
757 252 141 71
BamHI G'GATC_C
Cuts at: 0 136 1221
Size: 136 1085
BanI G'GyrC_C
Cuts at: 0 397 780 857 1221
Size: 397 383 77 364
Fragments arranged by size:
397 383 364 77
BanII G_rGCy'C
Cuts at: 0 85 200 1221
Size: 85 115 1021
Fragments arranged by size:
1021 115 85
BbeI G_GCGC'C
Cuts at: 0 401 1221
Size: 401 820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 881 1221
Size: 847 34 340
Fragments arranged by size:
847 340 34
BglI GCCn_nnn'nGGC
Cuts at: 0 706 944 1018 1221
Size: 706 238 74 203
Fragments arranged by size:
706 238 203 74
BlpI GC'TnA_GC
Cuts at: 0 1129 1221
Size: 1129 92
Bme1580I G_kGCm'C
Cuts at: 0 1190 1221
Size: 1190 31
BmgBI CAC'GTC
Cuts at: 0 747 1185 1221
Size: 747 438 36
Fragments arranged by size:
747 438 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 586 901 1221
Size: 244 342 315 320
Fragments arranged by size:
342 320 315 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 646 1221
Size: 267 379 575
Fragments arranged by size:
575 379 267
BsaHI Gr'CG_yC
Cuts at: 0 103 398 1221
Size: 103 295 823
Fragments arranged by size:
823 295 103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 450 1032 1219 1221
Size: 208 242 582 187 2
Fragments arranged by size:
582 242 208 187 2
BseYI C'CCAG_C
Cuts at: 0 24 63 843 1008 1221
Size: 24 39 780 165 213
Fragments arranged by size:
780 213 165 39 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 769 853 1221
Size: 313 456 84 368
Fragments arranged by size:
456 368 313 84
BsiEI CG_ry'CG
Cuts at: 0 18 56 204 357 1221
Size: 18 38 148 153 864
Fragments arranged by size:
864 153 148 38 18
BsiHKAI G_wGCw'C
Cuts at: 0 200 1135 1221
Size: 200 935 86
Fragments arranged by size:
935 200 86
BsmI GAATG_Cn'
Cuts at: 0 863 1115 1221
Size: 863 252 106
Fragments arranged by size:
863 252 106
Bsp1286I G_dGCh'C
Cuts at: 0 85 200 1135 1190 1221
Size: 85 115 935 55 31
Fragments arranged by size:
935 115 85 55 31
BsrBI CCG'CTC
Cuts at: 0 720 729 1221
Size: 720 9 492
Fragments arranged by size:
720 492 9
BsrFI r'CCGG_y
Cuts at: 0 55 269 578 783 815 973 1221
Size: 55 214 309 205 32 158 248
Fragments arranged by size:
309 248 214 205 158 55 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 1221
Size: 291 930
BstYI r'GATC_y
Cuts at: 0 136 365 1221
Size: 136 229 856
Fragments arranged by size:
856 229 136
Bsu36I CC'TnA_GG
Cuts at: 0 71 296 1221
Size: 71 225 925
Fragments arranged by size:
925 225 71
BtgI C'CryG_G
Cuts at: 0 305 621 776 1221
Size: 305 316 155 445
Fragments arranged by size:
445 316 305 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 902 1221
Size: 902 319
BtsI GCAGTG_nn'
Cuts at: 0 757 1221
Size: 757 464
DraIII CAC_nnn'GTG
Cuts at: 0 291 1221
Size: 291 930
EaeI y'GGCC_r
Cuts at: 0 53 187 201 354 381 405 773 891
Size: 53 134 14 153 27 24 368 118
Cuts at: 891 1221
Size: 330
Fragments arranged by size:
368 330 153 134 118 53 27 24
14
EagI C'GGCC_G
Cuts at: 0 53 201 354 1221
Size: 53 148 153 867
Fragments arranged by size:
867 153 148 53
EarI CTCTTCn'nnn_
Cuts at: 0 643 800 1221
Size: 643 157 421
Fragments arranged by size:
643 421 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 460 597 925 1221
Size: 460 137 328 296
Fragments arranged by size:
460 328 296 137
EcoICRI GAG'CTC
Cuts at: 0 198 1221
Size: 198 1023
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 586 901 1221
Size: 244 342 315 320
Fragments arranged by size:
342 320 315 244
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 1221
Size: 568 653
EcoO109I rG'GnC_Cy
Cuts at: 0 67 1026 1221
Size: 67 959 195
Fragments arranged by size:
959 195 67
FseI GG_CCGG'CC
Cuts at: 0 273 1221
Size: 273 948
FspI TGC'GCA
Cuts at: 0 322 1221
Size: 322 899
HaeII r_GCGC'y
Cuts at: 0 25 401 1221
Size: 25 376 820
Fragments arranged by size:
820 376 25
KasI G'GCGC_C
Cuts at: 0 397 1221
Size: 397 824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1221
Size: 515 706
MslI CAynn'nnrTG
Cuts at: 0 1057 1141 1198 1221
Size: 1057 84 57 23
Fragments arranged by size:
1057 84 57 23
MspA1I CmG'CkG
Cuts at: 0 671 761 1004 1221
Size: 671 90 243 217
Fragments arranged by size:
671 243 217 90
NaeI GCC'GGC
Cuts at: 0 57 271 580 1221
Size: 57 214 309 641
Fragments arranged by size:
641 309 214 57
NarI GG'CG_CC
Cuts at: 0 398 1221
Size: 398 823
NcoI C'CATG_G
Cuts at: 0 305 1221
Size: 305 916
NgoMIV G'CCGG_C
Cuts at: 0 55 269 578 1221
Size: 55 214 309 643
Fragments arranged by size:
643 309 214 55
NspI r_CATG'y
Cuts at: 0 1080 1221
Size: 1080 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 533 771 1221
Size: 533 238 450
Fragments arranged by size:
533 450 238
PfoI T'CCnGG_A
Cuts at: 0 221 563 608 1221
Size: 221 342 45 613
Fragments arranged by size:
613 342 221 45
PpuMI rG'GwC_Cy
Cuts at: 0 1026 1221
Size: 1026 195
PstI C_TGCA'G
Cuts at: 0 260 1221
Size: 260 961
PvuII CAG'CTG
Cuts at: 0 761 1004 1221
Size: 761 243 217
Fragments arranged by size:
761 243 217
SacI G_AGCT'C
Cuts at: 0 200 1221
Size: 200 1021
SanDI GG'GwC_CC
Cuts at: 0 1026 1221
Size: 1026 195
SfcI C'TryA_G
Cuts at: 0 256 1221
Size: 256 965
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 706 1221
Size: 706 515
SfoI GGC'GCC
Cuts at: 0 399 1221
Size: 399 822
SmaI CCC'GGG
Cuts at: 0 143 395 466 1221
Size: 143 252 71 755
Fragments arranged by size:
755 252 143 71
SmlI C'TyrA_G
Cuts at: 0 246 661 1221
Size: 246 415 560
Fragments arranged by size:
560 415 246
SphI G_CATG'C
Cuts at: 0 1080 1221
Size: 1080 141
SrfI GCCC'GGGC
Cuts at: 0 395 1221
Size: 395 826
StuI AGG'CCT
Cuts at: 0 76 709 878 1221
Size: 76 633 169 343
Fragments arranged by size:
633 343 169 76
StyI C'CwwG_G
Cuts at: 0 305 1221
Size: 305 916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 102 984 1221
Size: 102 882 237
Fragments arranged by size:
882 237 102
TatI w'GTAC_w
Cuts at: 0 602 1148 1163 1221
Size: 602 546 15 58
Fragments arranged by size:
602 546 58 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 978 1010 1221
Size: 978 32 211
Fragments arranged by size:
978 211 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1197 1221
Size: 1197 24
XmaI C'CCGG_G
Cuts at: 0 141 393 464 1221
Size: 141 252 71 757
Fragments arranged by size:
757 252 141 71
Enzymes that do cut:
AleI AlwNI AvaI BamHI BanI BanII BbeI BcgI
BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrFI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
FspI HaeII KasI MmeI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PpuMI PstI PvuII
SacI SanDI SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI
BclI BfrBI BglII BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI