Restriction map for
pFN21AB6543
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AciI 675 CCGC CCGC(-3/-1)
AcsI 866 GAATTC [GA]^AATT[CT]
AcyI 84 GACGCC G[GA]^CG[CT]C
AflIII 656 ACACGT A^C[GA][CT]GT
AlwNI 445 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 866 GAATTC [GA]^AATT[CT]
Asp700I 759 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 100 GAAGAC GAAGAC(2/6)
BlpI 700 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 700 GCTCAGC GC^T[ACGT]AGC
BpuAI 100 GAAGAC GAAGAC(2/6)
BsaHI 84 GACGCC G[GA]^CG[CT]C
BsaWI 566 ACCGGA [AT]^CCGG[AT]
BsmAI 417 GAGAC GTCTC(1/5)
BstAPI 552 GCACTTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 727 CCAATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 700 GCTCAGC GC^T[ACGT]AGC
EcoRI 866 GAATTC G^AATTC
HapII 567 CCGG C^CGG
HgaI 84 GACGC GACGC(5/10)
Hin1I 84 GACGCC G[GA]^CG[CT]C
HpaII 567 CCGG C^CGG
HphI 453 GGTGA GGTGA(8/7)
MaeII 658 ACGT A^CGT
MseI 680 TTAA T^TAA
MspI 567 CCGG C^CGG
NcoI 806 CCATGG C^CATGG
PpuMI 398 AGGACCC [GA]G^G[AT]CC[CT]
TaqI 200 TCGA T^CGA
Tru9I 680 TTAA T^TAA
TthHB8I 200 TCGA T^CGA
XmnI 759 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 1019 GGGCCC GGGCC^C
1073 GGGCCC
AspHI 550 GAGCAC G[AT]GC[AT]^C
698 GTGCTC
AvaII 399 GGACC G^G[AT]CC
579 GGACC
BsgI 303 GTGCAG GTGCAG(16/14)
559 GTGCAG
BsiHKAI 550 GAGCAC G[AT]GC[AT]^C
698 GTGCTC
BsmFI 578 GGGAC GGGAC(10/14)
945 GGGAC
BstF5I 26 CATCC GGATG(2/0)
639 GGATG
BstYI 362 AGATCC [GA]^GATC[CT]
891 GGATCT
DraIII 553 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
686 CACTCTGTG
EcoNI 168 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1023 CCTTGGGGAGG
EcoT14I 806 CCATGG C^C[AT][AT]GG
1023 CCTTGG
FokI 26 CATCC GGATG(9/13)
639 GGATG
MaeIII 898 GTTAC ^GT[ACGT]AC
1085 GTTAC
MflI 362 AGATCC [GA]^GATC[CT]
891 GGATCT
MslI 426 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
706 CATTGGAGTG
PflMI 540 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
685 CCACTCTGTGG
SfaNI 432 GATGC GCATC(5/9)
858 GATGC
StyI 806 CCATGG C^C[AT][AT]GG
1023 CCTTGG
Van91I 540 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
685 CCACTCTGTGG
XhoII 362 AGATCC [GA]^GATC[CT]
891 GGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI BanII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI HaeIII HinfI
ItaI MaeI MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TfiI
TseI Tsp509I TspRI