Restriction map for pFN21AB6543
 (Circular) (Six-base) MAPSORT of: pFN21AB6543.seq  Check: 934  from: 1  to: 5812



pFN21AB6543  5812 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3622     278 
   Size:         53      83     186     936    2086    2468

  Fragments arranged by size: 

                2468    2086     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3155    1937 
   Size:       1218    4594
 
Acc65I G'GTAC_C

Cuts at:   3133    3133 
   Size:       5812
 
AclI AA'CG_TT

Cuts at:   1563    3939    4312    1563 
   Size:       2376     373    3063

  Fragments arranged by size: 

                3063    2376     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2349    2903    3875    5259    5527    1632 
   Size:        717     554     972    1384     268    1917

  Fragments arranged by size: 

                1917    1384     972     717     554     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5615
 
AflIII A'CryG_T

Cuts at:   1203    2661    4711    5688    1203 
   Size:       1458    2050     977    1327

  Fragments arranged by size: 

                2050    1458    1327     977
 
AgeI A'CCGG_T

Cuts at:   4643    4643 
   Size:       5812
 
AhdI GACnn_n'nnGTC

Cuts at:   4541    4541 
   Size:       5812
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5812
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5812
 
AlwNI CAG_nnn'CTG

Cuts at:   2455    3555    5127    2455 
   Size:       1100    1572    3140

  Fragments arranged by size: 

                3140    1572    1100
 
ApaI G_GGCC'C

Cuts at:   3028    3082    3028 
   Size:         54    5758
 
ApaLI G'TGCA_C

Cuts at:   3869    5025    3869 
   Size:       1156    4656
 
ApoI r'AATT_y

Cuts at:   1493    2871    3121    3347    3499    5608    1493 
   Size:       1378     250     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1378     250     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4366     160 
   Size:        872    3334    1606

  Fragments arranged by size: 

                3334    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5812
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3137    1870 
   Size:         73    1194    4545

  Fragments arranged by size: 

                4545    1194      73
 
BamHI G'GATC_C

Cuts at:   3142    3509    3142 
   Size:        367    5445
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3133    4588     618 
   Size:        325     217     627    1346    1455    1842

  Fragments arranged by size: 

                1842    1455    1346     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2244    2473    3028    3082    3131    1391 
   Size:        853     229     555      54      49    4072

  Fragments arranged by size: 

                4072     853     555     229      54      49
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2112     928 
   Size:        971     213    4628

  Fragments arranged by size: 

                4628     971     213
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4003    4037    1903 
   Size:         34    2066      34    3678

  Fragments arranged by size: 

                3678    2066      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3704    4914    3704 
   Size:       1210    4602
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5812
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4423     136 
   Size:        107     122      71     631     605    2751    1525

  Fragments arranged by size: 

                2751    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5807    5807 
   Size:       5812
 
BlpI GC'TnA_GC

Cuts at:   2706    3229    2706 
   Size:        523    5289
 
Bme1580I G_kGCm'C

Cuts at:   1790    3028    3082    3873    5029    1790 
   Size:       1238      54     791    1156    2573

  Fragments arranged by size: 

                2573    1238    1156     791      54
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5812
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2590    2749    3085 
   Size:        416      53      83     399      24    1173     159     336

Cuts at:   3085    3543    4501     442 
   Size:        458     958    1753

  Fragments arranged by size: 

                1753    1173     958     458     416     399     336     159
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5812
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2384    2457    2953    4472    1239 
   Size:       1145      73     496    1519    2579

  Fragments arranged by size: 

                2579    1519    1145     496      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3937    4802    5100    3937 
   Size:        865     298    4649

  Fragments arranged by size: 

                4649     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4475     882 
   Size:        617    2976    2219

  Fragments arranged by size: 

                2976    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3603     493 
   Size:        711    2399    2702

  Fragments arranged by size: 

                2702    2399     711
 
BsaBI GATnn'nnATC

Cuts at:   5806    5806 
   Size:       5812
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2090    3619    4001    4636 
   Size:         53      83     186     936     557    1529     382     635

Cuts at:   4636     275 
   Size:       1451

  Fragments arranged by size: 

                1529    1451     936     635     557     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2571    4245    4643    4917    5064    5475    2571 
   Size:       1674     398     274     147     411    2908

  Fragments arranged by size: 

                2908    1674     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5587

  Fragments arranged by size: 

                5587     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5812
 
BseYI C'CCAG_C

Cuts at:   2059    2244    2508    5015    5511    2059 
   Size:        185     264    2507     496    2360

  Fragments arranged by size: 

                2507    2360     496     264     185
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2329    2585    1163 
   Size:        573     593     256    4390

  Fragments arranged by size: 

                4390     593     573     256
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3262    4023    5051    1540 
   Size:        275     186    1261     761    1028    2301

  Fragments arranged by size: 

                2301    1261    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2559    2707    3873    3958    5029    2559 
   Size:        148    1166      85    1071    3342

  Fragments arranged by size: 

                3342    1166    1071     148      85
 
BsmI GAATG_Cn'

Cuts at:   3323    3416    3323 
   Size:         93    5719
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2244    2473    2559    2707    3028    3082    3131 
   Size:        399     454     229      86     148     321      54      49

Cuts at:   3131    3873    3958    5029    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     454     399     321     229     148
                  86      85      54      49
 
BspEI T'CCGG_A

Cuts at:   5475    5475 
   Size:       5812
 
BspHI T'CATG_A

Cuts at:   3701    5431    3701 
   Size:       1730    4082
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3167     844 
   Size:       2323    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3699    1394 
   Size:        429    1876    3507

  Fragments arranged by size: 

                3507    1876     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4307    4481      65 
   Size:       1183    3059     174    1396

  Fragments arranged by size: 

                3059    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4456    4643    1700 
   Size:        110    2646     187    2869

  Fragments arranged by size: 

                2869    2646     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5812
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5812
 
BssSI C'ACGA_G

Cuts at:   3872    4884    3872 
   Size:       1012    4800
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2563     794 
   Size:       1769    4043
 
BstBI TT'CG_AA

Cuts at:   4616    4616 
   Size:       5812
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5812
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2739    2739 
   Size:       5812
 
BstYI r'GATC_y

Cuts at:   1972    2367    2896    3142    3509    3894    3911    5363    5449 
   Size:        395     529     246     367     385      17    1452      86

Cuts at:   5449    5461    5807    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     529     395     385     367     346     246
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2408    2790    2811    2914     513 
   Size:        552    1343     382      21     103    3411

  Fragments arranged by size: 

                3411    1343     552     382     103      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5073

  Fragments arranged by size: 

                5073     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3328    4122    4142    3328 
   Size:        794      20    4998

  Fragments arranged by size: 

                4998     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5812
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5777
 
DraI TTT'AAA

Cuts at:   3116    3116 
   Size:       5812
 
DraIII CAC_nnn'GTG

Cuts at:   2563    2696    2563 
   Size:        133    5679
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4819     809 
   Size:        507    3503    1802

  Fragments arranged by size: 

                3503    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3259    4148    5777 
   Size:         54    1425      50     275      18    1429     889    1629

Cuts at:   5777       8 
   Size:         43

  Fragments arranged by size: 

                1629    1429    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3259    1537 
   Size:        275    1447    4090

  Fragments arranged by size: 

                4090    1447     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3742    1353 
   Size:       2389    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4399    4773    4919     387 
   Size:       1064     138    2810     374     146    1280

  Fragments arranged by size: 

                2810    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2349    2384    2457    2903    2953    3875    4472 
   Size:        393     717      35      73     446      50     922     597

Cuts at:   4472    5259    5527    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     922     787     717     597     446     393     268
                  73      50      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2177    3032    2177 
   Size:        855    4957
 
EcoO109I rG'GnC_Cy

Cuts at:   2404    3024    3025    3038    3045    3079    2404 
   Size:        620       1      13       7      34    5137

  Fragments arranged by size: 

                5137     620      34      13       7       1
 
EcoRI G'AATT_C

Cuts at:   2871    3121    3499    2871 
   Size:        250     378    5184

  Fragments arranged by size: 

                5184     378     250
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2647    2647 
   Size:       5812
 
FspI TGC'GCA

Cuts at:   4318    4318 
   Size:       5812
 
HaeII r_GCGC'y

Cuts at:   1678    4959    1678 
   Size:       3281    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2808    2840    2979    3011    4453 
   Size:         32     135      32    1236      32     139      32    1442

Cuts at:   4453    4485    4527    4559    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1442    1236     139     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3156    3402     669 
   Size:        988     281    1218     246    3079

  Fragments arranged by size: 

                3079    1218     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5812
 
HpaI GTT'AAC

Cuts at:   3402    3402 
   Size:       5812
 
KpnI G_GTAC'C

Cuts at:   3137    3137 
   Size:       5812
 
MfeI C'AATT_G

Cuts at:   3411    3411 
   Size:       5812
 
MluI A'CGCG_T

Cuts at:   5688    5688 
   Size:       5812
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4926    5110    1305 
   Size:       3621     184    2007

  Fragments arranged by size: 

                3621    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3990

  Fragments arranged by size: 

                3990    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2435    2715    3770    4129    4288     518 
   Size:        648    1269     280    1055     359     159    2042

  Fragments arranged by size: 

                2042    1269    1055     648     359     280     159
 
MspA1I CmG'CkG

Cuts at:   1606    2050    2117    2592    3228    3905    5053    5298    1606 
   Size:        444      67     475     636     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     636     475     444     245      67
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2811     513 
   Size:        552    1746    3514

  Fragments arranged by size: 

                3514    1746     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5812
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5812
 
NotI GC'GGCC_GC

Cuts at:   3259    3259 
   Size:       5812
 
NspI r_CATG'y

Cuts at:   1132    1229    3170    4715    1132 
   Size:         97    1941    1545    2229

  Fragments arranged by size: 

                2229    1941    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2466    1348 
   Size:       1118    4694
 
PciI A'CATG_T

Cuts at:   4711    4711 
   Size:       5812
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2551    2696    1108 
   Size:       1443     145    4224

  Fragments arranged by size: 

                4224    1443     145
 
PfoI T'CCnGG_A

Cuts at:   2400    2400 
   Size:       5812
 
PmeI GTTT'AAAC

Cuts at:   3116    3116 
   Size:       5812
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2219    2251    3881    3913    5394    5426    2219 
   Size:         32    1630      32    1481      32    2605

  Fragments arranged by size: 

                2605    1630    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2404    2404 
   Size:       5812
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5812
 
PsiI TTA'TAA

Cuts at:   3382    3382 
   Size:       5812
 
PspOMI G'GGCC_C

Cuts at:   3024    3078    3024 
   Size:         54    5758
 
PstI C_TGCA'G

Cuts at:    830    2049    2451    2970    3164     830 
   Size:       1219     402     519     194    3478

  Fragments arranged by size: 

                3478    1219     519     402     194
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5812
 
PvuII CAG'CTG

Cuts at:   2050    2117    2592    2050 
   Size:         67     475    5270

  Fragments arranged by size: 

                5270     475      67
 
SalI G'TCGA_C

Cuts at:   1936    3154    1936 
   Size:       1218    4594
 
SbfI CC_TGCA'GG

Cuts at:   3164    3164 
   Size:       5812
 
ScaI AGT'ACT

Cuts at:   4060    5772    4060 
   Size:       1712    4100
 
SfcI C'TryA_G

Cuts at:    826    1045    2045    2447    2966    2977    3160    4295    4976 
   Size:        219    1000     402     519      11     183    1135     681

Cuts at:   4976    5167     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1000     681     519     402     219     191
                 183      11
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5812
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5812
 
SmaI CCC'GGG

Cuts at:   1872    3139    1872 
   Size:       1267    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3916    4817    5079     820 
   Size:        197     926    1973     901     262    1553

  Fragments arranged by size: 

                1973    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3603     493 
   Size:       3110    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5812
 
SphI G_CATG'C

Cuts at:   1132    3170    1132 
   Size:       2038    3774
 
SspI AAT'ATT

Cuts at:      5      52    3736       5 
   Size:         47    3684    2081

  Fragments arranged by size: 

                3684    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5812
 
StyI C'CwwG_G

Cuts at:    513    1065    2811    3028    3251     513 
   Size:        552    1746     217     223    3074

  Fragments arranged by size: 

                3074    1746     552     223     217
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2745    3839    3856    4009    4194    4639     325 
   Size:       1255    1165    1094      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1165    1094     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2208    4058    5770 
   Size:        274      80      33      51    1443     231    1850    1712

Cuts at:   5770      96 
   Size:        138

  Fragments arranged by size: 

                1850    1712    1443     274     231     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5772    5804    5772 
   Size:         32    5780
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5812
 
XbaI T'CTAG_A

Cuts at:   3148    3148 
   Size:       5812
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5812
 
XmaI C'CCGG_G

Cuts at:   1870    3137    1870 
   Size:       1267    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2768    3941    2768 
   Size:       1173    4639
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3620     276 
   Size:         53      83     186     936    2086    2468

  Fragments arranged by size: 

                2468    2086     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI     PstI     PvuI    PvuII     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I  EcoICRI
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI    PspXI     PsrI    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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