(Circular) (Six-base) MAPSORT of: pFN21AB6543.seq Check: 934 from: 1 to: 5812 pFN21AB6543 5812 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 1937 Size: 1218 4594 Acc65I G'GTAC_C Cuts at: 3133 3133 Size: 5812 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2349 2903 3875 5259 5527 1632 Size: 717 554 972 1384 268 1917 Fragments arranged by size: 1917 1384 972 717 554 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2661 4711 5688 1203 Size: 1458 2050 977 1327 Fragments arranged by size: 2050 1458 1327 977 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 2455 3555 5127 2455 Size: 1100 1572 3140 Fragments arranged by size: 3140 1572 1100 ApaI G_GGCC'C Cuts at: 3028 3082 3028 Size: 54 5758 ApaLI G'TGCA_C Cuts at: 3869 5025 3869 Size: 1156 4656 ApoI r'AATT_y Cuts at: 1493 2871 3121 3347 3499 5608 1493 Size: 1378 250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1378 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 3137 1870 Size: 73 1194 4545 Fragments arranged by size: 4545 1194 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3133 4588 618 Size: 325 217 627 1346 1455 1842 Fragments arranged by size: 1842 1455 1346 627 325 217 BanII G_rGCy'C Cuts at: 1391 2244 2473 3028 3082 3131 1391 Size: 853 229 555 54 49 4072 Fragments arranged by size: 4072 853 555 229 54 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2112 928 Size: 971 213 4628 Fragments arranged by size: 4628 971 213 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4423 136 Size: 107 122 71 631 605 2751 1525 Fragments arranged by size: 2751 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 2706 3229 2706 Size: 523 5289 Bme1580I G_kGCm'C Cuts at: 1790 3028 3082 3873 5029 1790 Size: 1238 54 791 1156 2573 Fragments arranged by size: 2573 1238 1156 791 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5812 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2590 2749 3085 Size: 416 53 83 399 24 1173 159 336 Cuts at: 3085 3543 4501 442 Size: 458 958 1753 Fragments arranged by size: 1753 1173 958 458 416 399 336 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2457 2953 4472 1239 Size: 1145 73 496 1519 2579 Fragments arranged by size: 2579 1519 1145 496 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3603 493 Size: 711 2399 2702 Fragments arranged by size: 2702 2399 711 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2090 3619 4001 4636 Size: 53 83 186 936 557 1529 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1529 1451 936 635 557 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2571 4245 4643 4917 5064 5475 2571 Size: 1674 398 274 147 411 2908 Fragments arranged by size: 2908 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5587 Fragments arranged by size: 5587 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5812 BseYI C'CCAG_C Cuts at: 2059 2244 2508 5015 5511 2059 Size: 185 264 2507 496 2360 Fragments arranged by size: 2507 2360 496 264 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 2585 1163 Size: 573 593 256 4390 Fragments arranged by size: 4390 593 573 256 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 2707 3873 3958 5029 2559 Size: 148 1166 85 1071 3342 Fragments arranged by size: 3342 1166 1071 148 85 BsmI GAATG_Cn' Cuts at: 3323 3416 3323 Size: 93 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2473 2559 2707 3028 3082 3131 Size: 399 454 229 86 148 321 54 49 Cuts at: 3131 3873 3958 5029 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 321 229 148 86 85 54 49 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 3701 5431 3701 Size: 1730 4082 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3167 844 Size: 2323 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4307 4481 65 Size: 1183 3059 174 1396 Fragments arranged by size: 3059 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3872 4884 3872 Size: 1012 4800 BstAPI GCAn_nnn'nTGC Cuts at: 794 2563 794 Size: 1769 4043 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5812 BstXI CCAn_nnnn'nTGG Cuts at: 2739 2739 Size: 5812 BstYI r'GATC_y Cuts at: 1972 2367 2896 3142 3509 3894 3911 5363 5449 Size: 395 529 246 367 385 17 1452 86 Cuts at: 5449 5461 5807 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 529 395 385 367 346 246 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2408 2790 2811 2914 513 Size: 552 1343 382 21 103 3411 Fragments arranged by size: 3411 1343 552 382 103 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 3328 4122 4142 3328 Size: 794 20 4998 Fragments arranged by size: 4998 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DraIII CAC_nnn'GTG Cuts at: 2563 2696 2563 Size: 133 5679 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777 Size: 54 1425 50 275 18 1429 889 1629 Cuts at: 5777 8 Size: 43 Fragments arranged by size: 1629 1429 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3742 1353 Size: 2389 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2349 2384 2457 2903 2953 3875 4472 Size: 393 717 35 73 446 50 922 597 Cuts at: 4472 5259 5527 1239 Size: 787 268 1524 Fragments arranged by size: 1524 922 787 717 597 446 393 268 73 50 35 EcoNI CCTnn'n_nnAGG Cuts at: 2177 3032 2177 Size: 855 4957 EcoO109I rG'GnC_Cy Cuts at: 2404 3024 3025 3038 3045 3079 2404 Size: 620 1 13 7 34 5137 Fragments arranged by size: 5137 620 34 13 7 1 EcoRI G'AATT_C Cuts at: 2871 3121 3499 2871 Size: 250 378 5184 Fragments arranged by size: 5184 378 250 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2647 2647 Size: 5812 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 4959 1678 Size: 3281 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2808 2840 2979 3011 4453 Size: 32 135 32 1236 32 139 32 1442 Cuts at: 4453 4485 4527 4559 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1442 1236 139 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 3402 669 Size: 988 281 1218 246 3079 Fragments arranged by size: 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KpnI G_GTAC'C Cuts at: 3137 3137 Size: 5812 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3990 Fragments arranged by size: 3990 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 2715 3770 4129 4288 518 Size: 648 1269 280 1055 359 159 2042 Fragments arranged by size: 2042 1269 1055 648 359 280 159 MspA1I CmG'CkG Cuts at: 1606 2050 2117 2592 3228 3905 5053 5298 1606 Size: 444 67 475 636 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 636 475 444 245 67 NcoI C'CATG_G Cuts at: 513 1065 2811 513 Size: 552 1746 3514 Fragments arranged by size: 3514 1746 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 3170 4715 1132 Size: 97 1941 1545 2229 Fragments arranged by size: 2229 1941 1545 97 PasI CC'CwG_GG Cuts at: 1348 2466 1348 Size: 1118 4694 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 2551 2696 1108 Size: 1443 145 4224 Fragments arranged by size: 4224 1443 145 PfoI T'CCnGG_A Cuts at: 2400 2400 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 2251 3881 3913 5394 5426 2219 Size: 32 1630 32 1481 32 2605 Fragments arranged by size: 2605 1630 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 2404 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PspOMI G'GGCC_C Cuts at: 3024 3078 3024 Size: 54 5758 PstI C_TGCA'G Cuts at: 830 2049 2451 2970 3164 830 Size: 1219 402 519 194 3478 Fragments arranged by size: 3478 1219 519 402 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2050 2117 2592 2050 Size: 67 475 5270 Fragments arranged by size: 5270 475 67 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SfcI C'TryA_G Cuts at: 826 1045 2045 2447 2966 2977 3160 4295 4976 Size: 219 1000 402 519 11 183 1135 681 Cuts at: 4976 5167 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1000 681 519 402 219 191 183 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 3139 1872 Size: 1267 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2811 3028 3251 513 Size: 552 1746 217 223 3074 Fragments arranged by size: 3074 1746 552 223 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2745 3839 3856 4009 4194 4639 325 Size: 1255 1165 1094 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1165 1094 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 4058 5770 Size: 274 80 33 51 1443 231 1850 1712 Cuts at: 5770 96 Size: 138 Fragments arranged by size: 1850 1712 1443 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5772 5804 5772 Size: 32 5780 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 3137 1870 Size: 1267 4545 XmnI GAAnn'nnTTC Cuts at: 2768 3941 2768 Size: 1173 4639 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI