Restriction map for
pFN21AB6543
(Linear) (Six-base) MAPSORT of: KIBB6543.seq Check: 8871 from: 1 to: 1107
KIBB6543.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 898 1107
Size: 344 554 209
Fragments arranged by size:
554 344 209
AflIII A'CryG_T
Cuts at: 0 656 1107
Size: 656 451
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1107
Size: 450 657
ApaI G_GGCC'C
Cuts at: 0 1023 1077 1107
Size: 1023 54 30
Fragments arranged by size:
1023 54 30
ApoI r'AATT_y
Cuts at: 0 866 1107
Size: 866 241
BanII G_rGCy'C
Cuts at: 0 239 468 1023 1077 1107
Size: 239 229 555 54 30
Fragments arranged by size:
555 239 229 54 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 1107
Size: 107 1000
BlpI GC'TnA_GC
Cuts at: 0 701 1107
Size: 701 406
Bme1580I G_kGCm'C
Cuts at: 0 1023 1077 1107
Size: 1023 54 30
Fragments arranged by size:
1023 54 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 744 1080 1107
Size: 585 159 336 27
Fragments arranged by size:
585 336 159 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 452 948 1107
Size: 379 73 496 159
Fragments arranged by size:
496 379 159 73
BsaHI Gr'CG_yC
Cuts at: 0 85 1107
Size: 85 1022
BsaWI w'CCGG_w
Cuts at: 0 566 1107
Size: 566 541
BseYI C'CCAG_C
Cuts at: 0 54 239 503 1107
Size: 54 185 264 604
Fragments arranged by size:
604 264 185 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 580 1107
Size: 324 256 527
Fragments arranged by size:
527 324 256
BsiHKAI G_wGCw'C
Cuts at: 0 554 702 1107
Size: 554 148 405
Fragments arranged by size:
554 405 148
Bsp1286I G_dGCh'C
Cuts at: 0 239 468 554 702 1023 1077 1107
Size: 239 229 86 148 321 54 30
Fragments arranged by size:
321 239 229 148 86 54 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 558 1107
Size: 558 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 734 1107
Size: 734 373
BstYI r'GATC_y
Cuts at: 0 362 891 1107
Size: 362 529 216
Fragments arranged by size:
529 362 216
BtgI C'CryG_G
Cuts at: 0 403 785 806 909 1107
Size: 403 382 21 103 198
Fragments arranged by size:
403 382 198 103 21
DraIII CAC_nnn'GTG
Cuts at: 0 558 691 1107
Size: 558 133 416
Fragments arranged by size:
558 416 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 379 452 898 948 1107
Size: 344 35 73 446 50 159
Fragments arranged by size:
446 344 159 73 50 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1027 1107
Size: 172 855 80
Fragments arranged by size:
855 172 80
EcoO109I rG'GnC_Cy
Cuts at: 0 399 1019 1020 1033 1040 1074 1107
Size: 399 620 1 13 7 34 33
Fragments arranged by size:
620 399 34 33 13 7 1
EcoRI G'AATT_C
Cuts at: 0 866 1107
Size: 866 241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 642 1107
Size: 642 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 803 835 974 1006 1107
Size: 803 32 139 32 101
Fragments arranged by size:
803 139 101 32 32
MslI CAynn'nnrTG
Cuts at: 0 430 710 1107
Size: 430 280 397
Fragments arranged by size:
430 397 280
MspA1I CmG'CkG
Cuts at: 0 45 112 587 1107
Size: 45 67 475 520
Fragments arranged by size:
520 475 67 45
NcoI C'CATG_G
Cuts at: 0 806 1107
Size: 806 301
PasI CC'CwG_GG
Cuts at: 0 461 1107
Size: 461 646
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 691 1107
Size: 546 145 416
Fragments arranged by size:
546 416 145
PfoI T'CCnGG_A
Cuts at: 0 395 1107
Size: 395 712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 1107
Size: 214 32 861
Fragments arranged by size:
861 214 32
PpuMI rG'GwC_Cy
Cuts at: 0 399 1107
Size: 399 708
PspOMI G'GGCC_C
Cuts at: 0 1019 1073 1107
Size: 1019 54 34
Fragments arranged by size:
1019 54 34
PstI C_TGCA'G
Cuts at: 0 44 446 965 1107
Size: 44 402 519 142
Fragments arranged by size:
519 402 142 44
PvuII CAG'CTG
Cuts at: 0 45 112 587 1107
Size: 45 67 475 520
Fragments arranged by size:
520 475 67 45
SfcI C'TryA_G
Cuts at: 0 40 442 961 972 1107
Size: 40 402 519 11 135
Fragments arranged by size:
519 402 135 40 11
StyI C'CwwG_G
Cuts at: 0 806 1023 1107
Size: 806 217 84
Fragments arranged by size:
806 217 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 740 1107
Size: 740 367
TatI w'GTAC_w
Cuts at: 0 203 1107
Size: 203 904
XmnI GAAnn'nnTTC
Cuts at: 0 763 1107
Size: 763 344
Enzymes that do cut:
AcuI AflIII AlwNI ApaI ApoI BanII BbsI BlpI
Bme1580I BmrI BpmI BsaHI BsaWI BseYI BsgI BsiHKAI
Bsp1286I BstAPI BstXI BstYI BtgI DraIII Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I MslI MspA1I NcoI PasI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SfcI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI