Restriction map for
pFN21AB8870
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MboII MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 304 CCGC CCGC(-3/-1)
AcsI 355 GAATTC [GA]^AATT[CT]
ApoI 355 GAATTC [GA]^AATT[CT]
Asp700I 3 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 277 CCCGGG C^[CT]CG[GA]G
BanI 147 GGTGCC G^G[CT][GA]CC
BbvI 195 GCAGC GCAGC(8/12)
BcgI 95 CGATGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 148 GTGCCC G[AGT]GC[ACT]^C
BsaBI 183 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 273 GAGACC GGTCTC(1/5)
BsmAI 273 GAGAC GTCTC(1/5)
BsmFI 14 GTCCC GGGAC(10/14)
BsoBI 277 CCCGGG C^[CT]CG[GA]G
Bsp1286I 148 GTGCCC G[AGT]GC[ACT]^C
BspMI 221 ACCTGC ACCTGC(4/8)
DdeI 365 CTGAG C^T[ACGT]AG
DpnII 228 GATC ^GATC
DraIII 392 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 355 GAATTC G^AATTC
EcoRV 325 GATATC GAT^ATC
HinfI 238 GACTC G^A[ACGT]TC
MaeII 200 ACGT A^CGT
MamI 183 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 228 GATC ^GATC
MseI 370 TTAA T^TAA
MspA1I 304 CCGCTG C[AC]G^C[GT]G
MwoI 135 GCTCAAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 228 GATC ^GATC
NlaIII 114 CATG CATG^
NlaIV 147 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 51 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 238 GACTC GAGTC(4/5)
Sau3AI 228 GATC ^GATC
SmaI 277 CCCGGG CCC^GGG
SmlI 136 CTCAAG C^T[CT][GA]AG
TaqI 206 TCGA T^CGA
Tru9I 370 TTAA T^TAA
TseI 195 GCAGC G^C[AT]GC
Tsp509I 356 AATT ^AATT
TspRI 392 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 206 TCGA T^CGA
Van91I 51 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 277 CCCGGG C^CCGGG
XmaI 277 CCCGGG C^CCGGG
XmnI 3 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcyI 291 GGCGTC G[GA]^CG[CT]C
300 GACGCC
AluI 134 AGCT AG^CT
143 AGCT
BsaHI 291 GGCGTC G[GA]^CG[CT]C
300 GACGCC
BsiYI 51 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
315 CCTCAACACGG
BslI 51 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
315 CCTCAACACGG
Fnu4HI 195 GCAGC GC^[ACGT]GC
303 GCCGC
HapII 69 CCGG C^CGG
278 CCGG
HgaI 300 GACGC GACGC(5/10)
292 GCGTC
Hin1I 291 GGCGTC G[GA]^CG[CT]C
300 GACGCC
HpaII 69 CCGG C^CGG
278 CCGG
HphI 174 GGTGA GGTGA(8/7)
267 TCACC
ItaI 195 GCAGC GC^[ACGT]GC
303 GCCGC
MnlI 315 CCTC CCTC(7/6)
21 GAGG
MspI 69 CCGG C^CGG
278 CCGG
PstI 193 CTGCAG CTGCA^G
223 CTGCAG
SfcI 193 CTGCAG C^T[GA][CT]AG
223 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AvaII BcnI BsaJI BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII FokI
MvaI NciI Sau96I ScrFI SfaNI