Restriction map for
pFN21AB6389
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AviII AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Van91I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccII 595 CGCG CG^CG
AcsI 306 GAATTC [GA]^AATT[CT]
AflIII 87 ACACGT A^C[GA][CT]GT
AhdI 583 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 104 AGCT AG^CT
AlwI 61 GGATC GGATC(4/5)
AlwNI 491 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 306 GAATTC [GA]^AATT[CT]
Asp700I 72 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 583 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 256 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 130 CTCGAG C^[CT]CG[GA]G
BalI 549 TGGCCA TGG^CCA
BbrPI 88 CACGTG CAC^GTG
BcnI 287 CCGGG CC^[GC]GG
BfaI 190 CTAG C^TAG
BglI 330 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 641 AGATCT A^GATCT
BpmI 178 CTGGAG CTGGAG(16/14)
BsaAI 88 CACGTG [CT]AC^GT[GA]
BsaBI 669 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 798 GAGGAG GAGGAG(10/8)
BsoBI 130 CTCGAG C^[CT]CG[GA]G
BsrBI 788 GAGCGG CCGCTC(-3/-3)
BsrDI 455 GCAATG GCAATG(2/0)
BstAPI 490 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 595 CGCG CG^CG
BstXI 362 CCACTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 641 AGATCT [GA]^GATC[CT]
CfoI 756 GCGC GCG^C
Eam1105I 583 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 306 GAATTC G^AATTC
EcoT14I 334 CCAAGG C^C[AT][AT]GG
HhaI 756 GCGC GCG^C
HinP1I 756 GCGC G^CGC
HincII 136 GTCAAC GT[CT]^[GA]AC
HindII 136 GTCAAC GT[CT]^[GA]AC
MaeI 190 CTAG C^TAG
MamI 669 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 641 AGATCT [GA]^GATC[CT]
MluNI 549 TGGCCA TGG^CCA
MscI 549 TGGCCA TGG^CCA
MspA1I 763 CAGCGG C[AC]G^C[GT]G
MvnI 595 CGCG CG^CG
NciI 287 CCGGG CC^[GC]GG
NlaIII 253 CATG CATG^
PaeR7I 130 CTCGAG C^TCGAG
PflFI 256 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 88 CACGTG CAC^GTG
PmlI 88 CACGTG CAC^GTG
PshAI 717 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 141 CTGCAG CTGCA^G
SfcI 141 CTGCAG C^T[GA][CT]AG
SmlI 130 CTCGAG C^T[CT][GA]AG
StuI 812 AGGCCT AGG^CCT
StyI 334 CCAAGG C^C[AT][AT]GG
TfiI 198 GATTC G^A[AT]TC
Tsp509I 307 AATT ^AATT
Tth111I 256 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 189 TCTAGA T^CTAGA
XhoI 130 CTCGAG C^TCGAG
XhoII 641 AGATCT [GA]^GATC[CT]
XmnI 72 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 85 GTAC GT^AC
324 GTAC
BanI 405 GGTGCC G^G[CT][GA]CC
772 GGCACC
BanII 115 GAGCCC G[GA]GC[CT]^C
418 GAGCCC
BsgI 466 CTGCAC GTGCAG(16/14)
399 GTGCAG
BsmFI 126 GTCCC GGGAC(10/14)
705 GTCCC
BspMI 280 ACCTGC ACCTGC(4/8)
339 GCAGGT
DdeI 218 CTCAG C^T[ACGT]AG
621 CTCAG
DraII 19 AGGACCC [GA]G^G[ACGT]CC[CT]
124 GGGTCCC
DsaI 413 CCGTGG C^C[GA][CT]GG
450 CCGTGG
EarI 37 CTCTTC CTCTTC(1/4)
439 CTCTTC
EcoO109I 19 AGGACCC [GA]G^G[ACGT]CC[CT]
124 GGGTCCC
HapII 287 CCGG C^CGG
819 CCGG
HpaII 287 CCGG C^CGG
819 CCGG
Ksp632I 37 CTCTTC CTCTTC(1/4)
439 CTCTTC
MaeII 89 ACGT A^CGT
314 ACGT
MslI 363 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
684 CACGGTGGTG
MspI 287 CCGG C^CGG
819 CCGG
PleI 462 GACTC GAGTC(4/5)
298 GAGTC
PpuMI 19 AGGACCC [GA]G^G[AT]CC[CT]
124 GGGTCCC
RsaI 85 GTAC GT^AC
324 GTAC
TaqI 80 TCGA T^CGA
131 TCGA
Tsp45I 682 GTCAC ^GT[GC]AC
715 GTGAC
TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^
353 CACTG
TthHB8I 80 TCGA T^CGA
131 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BmyI BsaI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI