(Circular) (Six-base) MAPSORT of: pFN21AB6389.seq Check: 166 from: 1 to: 5530 pFN21AB6389 5530 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2294 2294 Size: 5530 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 3593 4977 5245 1632 Size: 558 1403 1384 268 1917 Fragments arranged by size: 1917 1403 1384 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 2092 4429 5406 1203 Size: 889 2337 977 1327 Fragments arranged by size: 2337 1327 977 889 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 2593 4259 2593 Size: 1666 3864 AleI CACnn'nnGTG Cuts at: 1166 2372 2693 1166 Size: 1206 321 4003 Fragments arranged by size: 4003 1206 321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 2501 3273 4845 2501 Size: 772 1572 3186 Fragments arranged by size: 3186 1572 772 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2311 2839 3065 3217 5326 1493 Size: 818 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 818 528 226 152 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2135 2855 1870 Size: 73 192 720 4545 Fragments arranged by size: 4545 720 192 73 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2410 2777 2851 4306 618 Size: 325 217 627 623 367 74 1455 1842 Fragments arranged by size: 1842 1455 627 623 367 325 217 74 BanII G_rGCy'C Cuts at: 1391 2124 2427 2849 1391 Size: 733 303 422 4072 Fragments arranged by size: 4072 733 422 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 1903 Size: 34 1784 34 3678 Fragments arranged by size: 3678 1784 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2341 4141 136 Size: 107 122 71 631 605 669 1800 1525 Fragments arranged by size: 1800 1525 669 631 605 122 107 71 BglII A'GATC_T Cuts at: 2646 5525 2646 Size: 2879 2651 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 2415 2780 3591 4747 1790 Size: 625 365 811 1156 2573 Fragments arranged by size: 2573 1156 811 625 365 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3261 4219 442 Size: 416 53 83 399 24 1844 958 1753 Fragments arranged by size: 1844 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 4190 1239 Size: 965 1986 2579 Fragments arranged by size: 2579 1986 965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3655 4520 4818 3655 Size: 865 298 4367 Fragments arranged by size: 4367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2248 2474 4193 882 Size: 617 681 68 226 1719 2219 Fragments arranged by size: 2219 1719 681 617 226 68 BsaAI yAC'GTr Cuts at: 493 1204 2095 3321 493 Size: 711 891 1226 2702 Fragments arranged by size: 2702 1226 891 711 BsaBI GATnn'nnATC Cuts at: 2678 5524 2678 Size: 2846 2684 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3337 3719 4354 275 Size: 53 83 186 936 1804 382 635 1451 Fragments arranged by size: 1804 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3963 4361 4635 4782 5193 3963 Size: 398 274 147 411 4300 Fragments arranged by size: 4300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5305 Fragments arranged by size: 5305 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2818 1186 Size: 1632 3898 BseYI C'CCAG_C Cuts at: 4733 5229 4733 Size: 496 5034 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2425 2456 1163 Size: 573 689 31 4237 Fragments arranged by size: 4237 689 573 31 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2980 3741 4769 1540 Size: 275 186 979 761 1028 2301 Fragments arranged by size: 2301 1028 979 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3591 3676 4747 3591 Size: 85 1071 4374 Fragments arranged by size: 4374 1071 85 BsmI GAATG_Cn' Cuts at: 3041 3134 3041 Size: 93 5437 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2415 2427 2780 2849 3591 3676 Size: 399 334 291 12 353 69 742 85 Cuts at: 3676 4747 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 353 334 291 85 69 12 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 3419 5149 3419 Size: 1730 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 2335 2885 844 Size: 1450 41 550 3489 Fragments arranged by size: 3489 1450 550 41 BsrBI CCG'CTC Cuts at: 1394 1823 2795 3417 1394 Size: 429 972 622 3507 Fragments arranged by size: 3507 972 622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 4025 4199 65 Size: 1183 1219 1558 174 1396 Fragments arranged by size: 1558 1396 1219 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 3590 4602 3590 Size: 1012 4518 BstAPI GCAn_nnn'nTGC Cuts at: 794 2501 794 Size: 1707 3823 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2374 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2646 2860 3227 3612 3629 5081 5167 5179 Size: 674 214 367 385 17 1452 86 12 Cuts at: 5179 5525 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 674 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2418 2455 513 Size: 552 1353 37 3588 Fragments arranged by size: 3588 1353 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4791 Fragments arranged by size: 4791 683 56 BtsI GCAGTG_nn' Cuts at: 2357 3046 3840 3860 2357 Size: 689 794 20 4027 Fragments arranged by size: 4027 794 689 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2834 2834 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4537 809 Size: 507 3221 1802 Fragments arranged by size: 3221 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2554 2749 2825 Size: 54 1425 50 275 18 724 195 76 Cuts at: 2825 2977 3866 5495 8 Size: 152 889 1629 43 Fragments arranged by size: 1629 1425 889 724 275 195 152 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2977 1537 Size: 275 1165 4090 Fragments arranged by size: 4090 1165 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2450 3460 1353 Size: 695 402 1010 3423 Fragments arranged by size: 3423 1010 695 402 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2204 3593 4190 4977 5245 1239 Size: 393 558 14 1389 597 787 268 1524 Fragments arranged by size: 1524 1389 787 597 558 393 268 14 EcoO109I rG'GnC_Cy Cuts at: 2025 2130 2025 Size: 105 5425 EcoRI G'AATT_C Cuts at: 2311 2839 3217 2311 Size: 528 378 4624 Fragments arranged by size: 4624 528 378 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 4677 1678 Size: 2999 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2579 2611 2645 Size: 32 135 32 472 32 503 32 34 Cuts at: 2645 2677 4171 4203 4245 4277 1373 Size: 32 1494 32 42 32 2626 Fragments arranged by size: 2626 1494 503 472 135 42 34 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2874 3120 669 Size: 988 281 205 731 246 3079 Fragments arranged by size: 3079 988 731 281 246 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 5530 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 2622 2643 4644 4828 1305 Size: 1035 282 21 2001 184 2007 Fragments arranged by size: 2007 2001 1035 282 184 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2556 10 Size: 54 1425 343 724 2984 Fragments arranged by size: 2984 1425 724 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 2693 3488 3847 4006 518 Size: 648 1206 321 795 359 159 2042 Fragments arranged by size: 2042 1206 795 648 359 321 159 MspA1I CmG'CkG Cuts at: 1606 2770 2946 3623 4771 5016 1606 Size: 1164 176 677 1148 245 2120 Fragments arranged by size: 2120 1164 1148 677 245 176 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4978 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2888 4433 1132 Size: 97 1659 1545 2229 Fragments arranged by size: 2229 1659 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5530 PciI A'CATG_T Cuts at: 4429 4429 Size: 5530 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5530 PfoI T'CCnGG_A Cuts at: 2062 2291 2062 Size: 229 5301 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PmlI CAC'GTG Cuts at: 2095 2095 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2130 2025 Size: 105 5425 PshAI GACnn'nnGTC Cuts at: 1613 2726 1613 Size: 1113 4417 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PspXI vC'TCGA_Gb Cuts at: 2135 2135 Size: 5530 PstI C_TGCA'G Cuts at: 830 2150 2882 830 Size: 1320 732 3478 Fragments arranged by size: 3478 1320 732 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SanDI GG'GwC_CC Cuts at: 2130 2130 Size: 5530 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 3778 5490 3778 Size: 1712 3818 SfcI C'TryA_G Cuts at: 826 1045 2146 2878 4013 4694 4885 826 Size: 219 1101 732 1135 681 191 1471 Fragments arranged by size: 1471 1135 1101 732 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2857 1872 Size: 985 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2135 3634 4535 4797 820 Size: 197 926 192 1499 901 262 1553 Fragments arranged by size: 1553 1499 926 901 262 197 192 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2888 1132 Size: 1756 3774 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 2819 1517 Size: 1302 4228 StyI C'CwwG_G Cuts at: 513 1065 2339 2969 513 Size: 552 1274 630 3074 Fragments arranged by size: 3074 1274 630 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 3557 3574 3727 3912 4357 325 Size: 1255 585 1392 17 153 185 445 1498 Fragments arranged by size: 1498 1392 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3776 5488 96 Size: 274 80 33 51 1443 1799 1712 138 Fragments arranged by size: 1799 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5490 5522 5490 Size: 32 5498 Tth111I GACn'n_nGTC Cuts at: 1316 2264 1316 Size: 948 4582 XbaI T'CTAG_A Cuts at: 2194 2866 2194 Size: 672 4858 XhoI C'TCGA_G Cuts at: 1943 2135 1943 Size: 192 5338 XmaI C'CCGG_G Cuts at: 1870 2855 1870 Size: 985 4545 XmnI GAAnn'nnTTC Cuts at: 2081 3659 2081 Size: 1578 3952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI