Restriction map for pFN21AB6389
 (Circular) (Six-base) MAPSORT of: pFN21AB6389.seq  Check: 166  from: 1  to: 5530



pFN21AB6389  5530 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2294    2294 
   Size:       5530
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3340     278 
   Size:         53      83     186     936    1804    2468

  Fragments arranged by size: 

                2468    1804     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2873    1937 
   Size:        936    4594
 
Acc65I G'GTAC_C

Cuts at:   2851    2851 
   Size:       5530
 
AclI AA'CG_TT

Cuts at:   1563    3657    4030    1563 
   Size:       2094     373    3063

  Fragments arranged by size: 

                3063    2094     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2190    3593    4977    5245    1632 
   Size:        558    1403    1384     268    1917

  Fragments arranged by size: 

                1917    1403    1384     558     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5333
 
AflIII A'CryG_T

Cuts at:   1203    2092    4429    5406    1203 
   Size:        889    2337     977    1327

  Fragments arranged by size: 

                2337    1327     977     889
 
AgeI A'CCGG_T

Cuts at:   4361    4361 
   Size:       5530
 
AhdI GACnn_n'nnGTC

Cuts at:   2593    4259    2593 
   Size:       1666    3864
 
AleI CACnn'nnGTG

Cuts at:   1166    2372    2693    1166 
   Size:       1206     321    4003

  Fragments arranged by size: 

                4003    1206     321
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5530
 
AlwNI CAG_nnn'CTG

Cuts at:   2501    3273    4845    2501 
   Size:        772    1572    3186

  Fragments arranged by size: 

                3186    1572     772
 
ApaLI G'TGCA_C

Cuts at:   3587    4743    3587 
   Size:       1156    4374
 
ApoI r'AATT_y

Cuts at:   1493    2311    2839    3065    3217    5326    1493 
   Size:        818     528     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     818     528     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4084     160 
   Size:        872    3052    1606

  Fragments arranged by size: 

                3052    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5530
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2135    2855    1870 
   Size:         73     192     720    4545

  Fragments arranged by size: 

                4545     720     192      73
 
BamHI G'GATC_C

Cuts at:   2860    3227    2860 
   Size:        367    5163
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2410    2777    2851    4306     618 
   Size:        325     217     627     623     367      74    1455    1842

  Fragments arranged by size: 

                1842    1455     627     623     367     325     217      74
            
 
BanII G_rGCy'C

Cuts at:   1391    2124    2427    2849    1391 
   Size:        733     303     422    4072

  Fragments arranged by size: 

                4072     733     422     303
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4559
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3721    3755    1903 
   Size:         34    1784      34    3678

  Fragments arranged by size: 

                3678    1784      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3422    4632    3422 
   Size:       1210    4320
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5530
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2341    4141     136 
   Size:        107     122      71     631     605     669    1800    1525

  Fragments arranged by size: 

                1800    1525     669     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2646    5525    2646 
   Size:       2879    2651
 
BlpI GC'TnA_GC

Cuts at:   2947    2947 
   Size:       5530
 
Bme1580I G_kGCm'C

Cuts at:   1790    2415    2780    3591    4747    1790 
   Size:        625     365     811    1156    2573

  Fragments arranged by size: 

                2573    1156     811     625     365
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5530
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3261    4219     442 
   Size:        416      53      83     399      24    1844     958    1753

  Fragments arranged by size: 

                1844    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5530
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2204    4190    1239 
   Size:        965    1986    2579

  Fragments arranged by size: 

                2579    1986     965
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3655    4520    4818    3655 
   Size:        865     298    4367

  Fragments arranged by size: 

                4367     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2180    2248    2474    4193     882 
   Size:        617     681      68     226    1719    2219

  Fragments arranged by size: 

                2219    1719     681     617     226      68
 
BsaAI yAC'GTr

Cuts at:    493    1204    2095    3321     493 
   Size:        711     891    1226    2702

  Fragments arranged by size: 

                2702    1226     891     711
 
BsaBI GATnn'nnATC

Cuts at:   2678    5524    2678 
   Size:       2846    2684
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3337    3719    4354     275 
   Size:         53      83     186     936    1804     382     635    1451

  Fragments arranged by size: 

                1804    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3963    4361    4635    4782    5193    3963 
   Size:        398     274     147     411    4300

  Fragments arranged by size: 

                4300     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5305

  Fragments arranged by size: 

                5305     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2818    1186 
   Size:       1632    3898
 
BseYI C'CCAG_C

Cuts at:   4733    5229    4733 
   Size:        496    5034
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2425    2456    1163 
   Size:        573     689      31    4237

  Fragments arranged by size: 

                4237     689     573      31
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2980    3741    4769    1540 
   Size:        275     186     979     761    1028    2301

  Fragments arranged by size: 

                2301    1028     979     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3591    3676    4747    3591 
   Size:         85    1071    4374

  Fragments arranged by size: 

                4374    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3041    3134    3041 
   Size:         93    5437
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2124    2415    2427    2780    2849    3591    3676 
   Size:        399     334     291      12     353      69     742      85

Cuts at:   3676    4747    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     353     334     291      85
                  69      12
 
BspEI T'CCGG_A

Cuts at:   5193    5193 
   Size:       5530
 
BspHI T'CATG_A

Cuts at:   3419    5149    3419 
   Size:       1730    3800
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2294    2335    2885     844 
   Size:       1450      41     550    3489

  Fragments arranged by size: 

                3489    1450     550      41
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2795    3417    1394 
   Size:        429     972     622    3507

  Fragments arranged by size: 

                3507     972     622     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2467    4025    4199      65 
   Size:       1183    1219    1558     174    1396

  Fragments arranged by size: 

                1558    1396    1219    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4174    4361    1700 
   Size:        110    2364     187    2869

  Fragments arranged by size: 

                2869    2364     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5530
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5530
 
BssSI C'ACGA_G

Cuts at:   3590    4602    3590 
   Size:       1012    4518
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2501     794 
   Size:       1707    3823
 
BstBI TT'CG_AA

Cuts at:   4334    4334 
   Size:       5530
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5530
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2374    2374 
   Size:       5530
 
BstYI r'GATC_y

Cuts at:   1972    2646    2860    3227    3612    3629    5081    5167    5179 
   Size:        674     214     367     385      17    1452      86      12

Cuts at:   5179    5525    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     674     385     367     346     214      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2418    2455     513 
   Size:        552    1353      37    3588

  Fragments arranged by size: 

                3588    1353     552      37
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4791

  Fragments arranged by size: 

                4791     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2357    3046    3840    3860    2357 
   Size:        689     794      20    4027

  Fragments arranged by size: 

                4027     794     689      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5530
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5495
 
DraI TTT'AAA

Cuts at:   2834    2834 
   Size:       5530
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4537     809 
   Size:        507    3221    1802

  Fragments arranged by size: 

                3221    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2554    2749    2825 
   Size:         54    1425      50     275      18     724     195      76

Cuts at:   2825    2977    3866    5495       8 
   Size:        152     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     724     275     195     152      76
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2977    1537 
   Size:        275    1165    4090

  Fragments arranged by size: 

                4090    1165     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2048    2450    3460    1353 
   Size:        695     402    1010    3423

  Fragments arranged by size: 

                3423    1010     695     402
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4117    4491    4637     387 
   Size:       1064     138    2528     374     146    1280

  Fragments arranged by size: 

                2528    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2190    2204    3593    4190    4977    5245    1239 
   Size:        393     558      14    1389     597     787     268    1524

  Fragments arranged by size: 

                1524    1389     787     597     558     393     268      14
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2025    2130    2025 
   Size:        105    5425
 
EcoRI G'AATT_C

Cuts at:   2311    2839    3217    2311 
   Size:        528     378    4624

  Fragments arranged by size: 

                4624     528     378
 
FspI TGC'GCA

Cuts at:   4036    4036 
   Size:       5530
 
HaeII r_GCGC'y

Cuts at:   1678    4677    1678 
   Size:       2999    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2044    2076    2579    2611    2645 
   Size:         32     135      32     472      32     503      32      34

Cuts at:   2645    2677    4171    4203    4245    4277    1373 
   Size:         32    1494      32      42      32    2626

  Fragments arranged by size: 

                2626    1494     503     472     135      42      34      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2143    2874    3120     669 
   Size:        988     281     205     731     246    3079

  Fragments arranged by size: 

                3079     988     731     281     246     205
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5530
 
HpaI GTT'AAC

Cuts at:   3120    3120 
   Size:       5530
 
KpnI G_GTAC'C

Cuts at:   2855    2855 
   Size:       5530
 
MfeI C'AATT_G

Cuts at:   3129    3129 
   Size:       5530
 
MluI A'CGCG_T

Cuts at:   5406    5406 
   Size:       5530
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2340    2622    2643    4644    4828    1305 
   Size:       1035     282      21    2001     184    2007

  Fragments arranged by size: 

                2007    2001    1035     282     184      21
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2556      10 
   Size:         54    1425     343     724    2984

  Fragments arranged by size: 

                2984    1425     724     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2372    2693    3488    3847    4006     518 
   Size:        648    1206     321     795     359     159    2042

  Fragments arranged by size: 

                2042    1206     795     648     359     321     159
 
MspA1I CmG'CkG

Cuts at:   1606    2770    2946    3623    4771    5016    1606 
   Size:       1164     176     677    1148     245    2120

  Fragments arranged by size: 

                2120    1164    1148     677     245     176
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4978
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5530
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5530
 
NotI GC'GGCC_GC

Cuts at:   2977    2977 
   Size:       5530
 
NspI r_CATG'y

Cuts at:   1132    1229    2888    4433    1132 
   Size:         97    1659    1545    2229

  Fragments arranged by size: 

                2229    1659    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5530
 
PciI A'CATG_T

Cuts at:   4429    4429 
   Size:       5530
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5530
 
PfoI T'CCnGG_A

Cuts at:   2062    2291    2062 
   Size:        229    5301
 
PmeI GTTT'AAAC

Cuts at:   2834    2834 
   Size:       5530
 
PmlI CAC'GTG

Cuts at:   2095    2095 
   Size:       5530
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3599    3631    5112    5144    3599 
   Size:         32    1481      32    3985

  Fragments arranged by size: 

                3985    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2025    2130    2025 
   Size:        105    5425
 
PshAI GACnn'nnGTC

Cuts at:   1613    2726    1613 
   Size:       1113    4417
 
PsiI TTA'TAA

Cuts at:   3100    3100 
   Size:       5530
 
PspXI vC'TCGA_Gb

Cuts at:   2135    2135 
   Size:       5530
 
PstI C_TGCA'G

Cuts at:    830    2150    2882     830 
   Size:       1320     732    3478

  Fragments arranged by size: 

                3478    1320     732
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5530
 
SalI G'TCGA_C

Cuts at:   1936    2872    1936 
   Size:        936    4594
 
SanDI GG'GwC_CC

Cuts at:   2130    2130 
   Size:       5530
 
SbfI CC_TGCA'GG

Cuts at:   2882    2882 
   Size:       5530
 
ScaI AGT'ACT

Cuts at:   3778    5490    3778 
   Size:       1712    3818
 
SfcI C'TryA_G

Cuts at:    826    1045    2146    2878    4013    4694    4885     826 
   Size:        219    1101     732    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1101     732     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5530
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5530
 
SmaI CCC'GGG

Cuts at:   1872    2857    1872 
   Size:        985    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2135    3634    4535    4797     820 
   Size:        197     926     192    1499     901     262    1553

  Fragments arranged by size: 

                1553    1499     926     901     262     197     192
 
SnaBI TAC'GTA

Cuts at:    493    3321     493 
   Size:       2828    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5530
 
SphI G_CATG'C

Cuts at:   1132    2888    1132 
   Size:       1756    3774
 
SspI AAT'ATT

Cuts at:      5      52    3454       5 
   Size:         47    3402    2081

  Fragments arranged by size: 

                3402    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2819    1517 
   Size:       1302    4228
 
StyI C'CwwG_G

Cuts at:    513    1065    2339    2969     513 
   Size:        552    1274     630    3074

  Fragments arranged by size: 

                3074    1274     630     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2165    3557    3574    3727    3912    4357     325 
   Size:       1255     585    1392      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1392    1255     585     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3776    5488      96 
   Size:        274      80      33      51    1443    1799    1712     138

  Fragments arranged by size: 

                1799    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5490    5522    5490 
   Size:         32    5498
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2264    1316 
   Size:        948    4582
 
XbaI T'CTAG_A

Cuts at:   2194    2866    2194 
   Size:        672    4858
 
XhoI C'TCGA_G

Cuts at:   1943    2135    1943 
   Size:        192    5338
 
XmaI C'CCGG_G

Cuts at:   1870    2855    1870 
   Size:        985    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2081    3659    2081 
   Size:       1578    3952
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3338     276 
   Size:         53      83     186     936    1804    2468

  Fragments arranged by size: 

                2468    1804     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PstI     PvuI     SalI    SanDI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI   PspOMI     PsrI    PvuII
    RsrII     SacI    SacII     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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