Restriction map for
pFN21AB6389
(Linear) (Six-base) MAPSORT of: KIBB6389.seq Check: 8973 from: 1 to: 825
KIBB6389.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 289 825
Size: 289 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 825
Size: 185 640
AflIII A'CryG_T
Cuts at: 0 87 825
Size: 87 738
AhdI GACnn_n'nnGTC
Cuts at: 0 588 825
Size: 588 237
AleI CACnn'nnGTG
Cuts at: 0 367 688 825
Size: 367 321 137
Fragments arranged by size:
367 321 137
AlwNI CAG_nnn'CTG
Cuts at: 0 496 825
Size: 496 329
ApoI r'AATT_y
Cuts at: 0 306 825
Size: 306 519
AvaI C'yCGr_G
Cuts at: 0 130 825
Size: 130 695
BanI G'GyrC_C
Cuts at: 0 405 772 825
Size: 405 367 53
Fragments arranged by size:
405 367 53
BanII G_rGCy'C
Cuts at: 0 119 422 825
Size: 119 303 403
Fragments arranged by size:
403 303 119
BglI GCCn_nnn'nGGC
Cuts at: 0 336 825
Size: 336 489
BglII A'GATC_T
Cuts at: 0 641 825
Size: 641 184
Bme1580I G_kGCm'C
Cuts at: 0 410 775 825
Size: 410 365 50
Fragments arranged by size:
410 365 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 825
Size: 199 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 243 469 825
Size: 175 68 226 356
Fragments arranged by size:
356 226 175 68
BsaAI yAC'GTr
Cuts at: 0 90 825
Size: 90 735
BsaBI GATnn'nnATC
Cuts at: 0 673 825
Size: 673 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 825
Size: 813 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 451 825
Size: 420 31 374
Fragments arranged by size:
420 374 31
Bsp1286I G_dGCh'C
Cuts at: 0 119 410 422 775 825
Size: 119 291 12 353 50
Fragments arranged by size:
353 291 119 50 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 289 330 825
Size: 289 41 495
Fragments arranged by size:
495 289 41
BsrBI CCG'CTC
Cuts at: 0 790 825
Size: 790 35
BsrDI GCAATG_nn'
Cuts at: 0 462 825
Size: 462 363
BstAPI GCAn_nnn'nTGC
Cuts at: 0 496 825
Size: 496 329
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 825
Size: 369 456
BstYI r'GATC_y
Cuts at: 0 641 825
Size: 641 184
BtgI C'CryG_G
Cuts at: 0 413 450 825
Size: 413 37 375
Fragments arranged by size:
413 375 37
BtsI GCAGTG_nn'
Cuts at: 0 352 825
Size: 352 473
EaeI y'GGCC_r
Cuts at: 0 549 744 820 825
Size: 549 195 76 5
Fragments arranged by size:
549 195 76 5
EarI CTCTTCn'nnn_
Cuts at: 0 43 445 825
Size: 43 402 380
Fragments arranged by size:
402 380 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 199 825
Size: 185 14 626
Fragments arranged by size:
626 185 14
EcoO109I rG'GnC_Cy
Cuts at: 0 20 125 825
Size: 20 105 700
Fragments arranged by size:
700 105 20
EcoRI G'AATT_C
Cuts at: 0 306 825
Size: 306 519
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 39 71 574 606 640 672 825
Size: 39 32 503 32 34 32 153
Fragments arranged by size:
503 153 39 34 32 32 32
HincII GTy'rAC
Cuts at: 0 138 825
Size: 138 687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 617 638 825
Size: 335 282 21 187
Fragments arranged by size:
335 282 187 21
MscI TGG'CCA
Cuts at: 0 551 825
Size: 551 274
MslI CAynn'nnrTG
Cuts at: 0 367 688 825
Size: 367 321 137
Fragments arranged by size:
367 321 137
MspA1I CmG'CkG
Cuts at: 0 765 825
Size: 765 60
PfoI T'CCnGG_A
Cuts at: 0 57 286 825
Size: 57 229 539
Fragments arranged by size:
539 229 57
PmlI CAC'GTG
Cuts at: 0 90 825
Size: 90 735
PpuMI rG'GwC_Cy
Cuts at: 0 20 125 825
Size: 20 105 700
Fragments arranged by size:
700 105 20
PshAI GACnn'nnGTC
Cuts at: 0 721 825
Size: 721 104
PspXI vC'TCGA_Gb
Cuts at: 0 130 825
Size: 130 695
PstI C_TGCA'G
Cuts at: 0 145 825
Size: 145 680
SanDI GG'GwC_CC
Cuts at: 0 125 825
Size: 125 700
SfcI C'TryA_G
Cuts at: 0 141 825
Size: 141 684
SmlI C'TyrA_G
Cuts at: 0 130 825
Size: 130 695
StuI AGG'CCT
Cuts at: 0 814 825
Size: 814 11
StyI C'CwwG_G
Cuts at: 0 334 825
Size: 334 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 825
Size: 160 665
Tth111I GACn'n_nGTC
Cuts at: 0 259 825
Size: 259 566
XbaI T'CTAG_A
Cuts at: 0 189 825
Size: 189 636
XhoI C'TCGA_G
Cuts at: 0 130 825
Size: 130 695
XmnI GAAnn'nnTTC
Cuts at: 0 76 825
Size: 76 749
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApoI AvaI
BanI BanII BglI BglII Bme1580I BpmI BsaI BsaAI
BsaBI BseRI BsgI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstXI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I
EcoRI Hin4I HincII MmeI MscI MslI MspA1I PfoI
PmlI PpuMI PshAI PspXI PstI SanDI SfcI SmlI
StuI StyI TaqII Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XcmI XmaI ZraI