(Circular) (Six-base) MAPSORT of: pFN21AE0106.seq Check: 6644 from: 1 to: 5548 pFN21AE0106 5548 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3358 278 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2384 3611 4995 5263 1632 Size: 752 1227 1384 268 1917 Fragments arranged by size: 1917 1384 1227 752 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5548 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5548 AlwNI CAG_nnn'CTG Cuts at: 2130 2278 2428 2743 3291 4863 2130 Size: 148 150 315 548 1572 2815 Fragments arranged by size: 2815 1572 548 315 150 148 ApaI G_GGCC'C Cuts at: 2634 2634 Size: 5548 ApaLI G'TGCA_C Cuts at: 3605 4761 3605 Size: 1156 4392 ApoI r'AATT_y Cuts at: 1493 2720 2857 3083 3235 5344 1493 Size: 1227 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2873 1870 Size: 73 930 4545 Fragments arranged by size: 4545 930 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 2537 2869 4324 618 Size: 325 217 627 543 207 332 1455 1842 Fragments arranged by size: 1842 1455 627 543 332 325 217 207 BanII G_rGCy'C Cuts at: 1391 2236 2634 2867 1391 Size: 845 398 233 4072 Fragments arranged by size: 4072 845 398 233 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2462 928 Size: 971 563 4014 Fragments arranged by size: 4014 971 563 BbvCI CC'TCA_GC Cuts at: 2608 2608 Size: 5548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 1548 Size: 5548 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5543 5543 Size: 5548 BlpI GC'TnA_GC Cuts at: 2965 2965 Size: 5548 Bme1580I G_kGCm'C Cuts at: 1790 2634 3609 4765 1790 Size: 844 975 1156 2573 Fragments arranged by size: 2573 1156 975 844 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 3279 4237 Size: 416 53 83 399 24 849 1013 958 Cuts at: 4237 442 Size: 1753 Fragments arranged by size: 1753 1013 958 849 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 2792 4208 1239 Size: 888 665 1416 2579 Fragments arranged by size: 2579 1416 888 665 Bpu10I CC'TnA_GC Cuts at: 2240 2608 2240 Size: 368 5180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2303 2738 3673 4538 4836 2303 Size: 435 935 865 298 3015 Fragments arranged by size: 3015 935 865 435 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2032 2220 4211 882 Size: 617 533 188 1991 2219 Fragments arranged by size: 2219 1991 617 533 188 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2371 3355 3737 Size: 53 83 186 936 578 260 984 382 Cuts at: 3737 4372 275 Size: 635 1451 Fragments arranged by size: 1451 984 936 635 578 382 260 186 83 53 BsaWI w'CCGG_w Cuts at: 3981 4379 4653 4800 5211 3981 Size: 398 274 147 411 4318 Fragments arranged by size: 4318 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 1186 Size: 944 4604 BseYI C'CCAG_C Cuts at: 2612 2665 4751 5247 2612 Size: 53 2086 496 2913 Fragments arranged by size: 2913 2086 496 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 1163 Size: 573 528 4447 Fragments arranged by size: 4447 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2295 2998 3759 4787 1540 Size: 275 186 294 703 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 294 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2690 2778 3609 3694 4765 2162 Size: 528 88 831 85 1071 2945 Fragments arranged by size: 2945 1071 831 528 88 85 BsmI GAATG_Cn' Cuts at: 3059 3152 3059 Size: 93 5455 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2236 2634 2690 2778 2867 3609 Size: 399 372 74 398 56 88 89 742 Cuts at: 3609 3694 4765 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 372 89 88 85 74 56 BspEI T'CCGG_A Cuts at: 5211 5211 Size: 5548 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2903 844 Size: 1463 596 3489 Fragments arranged by size: 3489 1463 596 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4043 4217 65 Size: 1183 2795 174 1396 Fragments arranged by size: 2795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2253 4192 4379 1700 Size: 110 443 1939 187 2869 Fragments arranged by size: 2869 1939 443 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 2743 2782 794 Size: 1949 39 3560 Fragments arranged by size: 3560 1949 39 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstXI CCAn_nnnn'nTGG Cuts at: 2840 2840 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2553 2878 3245 3630 3647 5099 5185 5197 Size: 581 325 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 581 385 367 346 325 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2033 513 Size: 552 968 4028 Fragments arranged by size: 4028 968 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 2362 3064 3858 3878 2362 Size: 702 794 20 4032 Fragments arranged by size: 4032 794 702 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2625 2660 529 Size: 35 2061 35 3417 Fragments arranged by size: 3417 2061 35 35 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DraIII CAC_nnn'GTG Cuts at: 2782 2782 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2292 2437 2523 Size: 54 1425 50 275 18 462 145 86 Cuts at: 2523 2842 2995 3884 5513 8 Size: 319 153 889 1629 43 Fragments arranged by size: 1629 1425 889 462 319 275 153 145 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2292 2995 1537 Size: 275 480 703 4090 Fragments arranged by size: 4090 703 480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2487 3478 1353 Size: 1134 991 3423 Fragments arranged by size: 3423 1134 991 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2384 2792 3611 4208 4995 5263 Size: 393 495 257 408 819 597 787 268 Cuts at: 5263 1239 Size: 1524 Fragments arranged by size: 1524 819 787 597 495 408 393 268 257 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2282 2378 2145 Size: 137 96 5315 Fragments arranged by size: 5315 137 96 EcoO109I rG'GnC_Cy Cuts at: 2425 2596 2617 2631 2425 Size: 171 21 14 5342 Fragments arranged by size: 5342 171 21 14 EcoRI G'AATT_C Cuts at: 2720 2857 3235 2720 Size: 137 378 5033 Fragments arranged by size: 5033 378 137 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 2254 4695 1678 Size: 576 2441 2531 Fragments arranged by size: 2531 2441 576 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2464 2496 4189 4221 4263 Size: 32 135 32 892 32 1693 32 42 Cuts at: 4263 4295 1373 Size: 32 2626 Fragments arranged by size: 2626 1693 892 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2491 2530 2892 3138 669 Size: 988 281 553 39 362 246 3079 Fragments arranged by size: 3079 988 553 362 281 246 39 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2030 2634 4662 4846 1305 Size: 725 604 2028 184 2007 Fragments arranged by size: 2028 2007 725 604 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2439 2844 10 Size: 54 1425 343 607 405 2714 Fragments arranged by size: 2714 1425 607 405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3506 3865 4024 518 Size: 648 2340 359 159 2042 Fragments arranged by size: 2340 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2308 2512 2612 2665 2964 3641 4789 5034 Size: 702 204 100 53 299 677 1148 245 Cuts at: 5034 1606 Size: 2120 Fragments arranged by size: 2120 1148 702 677 299 245 204 100 53 NaeI GCC'GGC Cuts at: 2255 2255 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4996 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2253 2253 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2906 4451 1132 Size: 97 1677 1545 2229 Fragments arranged by size: 2229 1677 1545 97 PasI CC'CwG_GG Cuts at: 1348 2627 1348 Size: 1279 4269 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5548 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2596 2617 2425 Size: 171 21 5356 Fragments arranged by size: 5356 171 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PspOMI G'GGCC_C Cuts at: 2630 2630 Size: 5548 PstI C_TGCA'G Cuts at: 830 2066 2739 2900 830 Size: 1236 673 161 3478 Fragments arranged by size: 3478 1236 673 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2512 2612 2665 2512 Size: 100 53 5395 Fragments arranged by size: 5395 100 53 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SapI GCTCTTCn'nnn_ Cuts at: 2487 2487 Size: 5548 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2062 2660 2735 2896 4031 4712 4903 Size: 219 1017 598 75 161 1135 681 191 Cuts at: 4903 826 Size: 1471 Fragments arranged by size: 1471 1135 1017 681 598 219 191 161 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 2753 3652 4553 4815 820 Size: 197 926 375 435 899 901 262 1553 Fragments arranged by size: 1553 926 901 899 435 375 262 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2337 2987 513 Size: 552 1272 650 3074 Fragments arranged by size: 3074 1272 650 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2244 2285 2555 3575 3592 3745 3930 Size: 1255 664 41 270 1020 17 153 185 Cuts at: 3930 4375 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1020 664 445 270 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3794 5506 96 Size: 274 80 33 51 1443 1817 1712 138 Fragments arranged by size: 1817 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5508 5540 5508 Size: 32 5516 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3356 276 Size: 53 83 186 936 1822 2468 Fragments arranged by size: 2468 1822 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI