(Circular) (Six-base) MAPSORT of: pFN21AE0996.seq Check: 1113 from: 1 to: 6682 pFN21AE0996 6682 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4492 278 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4025 1937 Size: 2088 4594 Acc65I G'GTAC_C Cuts at: 4003 4003 Size: 6682 AclI AA'CG_TT Cuts at: 1563 4809 5182 1563 Size: 3246 373 3063 Fragments arranged by size: 3246 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2376 2835 3339 3855 4745 6129 6397 Size: 425 319 459 504 516 890 1384 268 Cuts at: 6397 1632 Size: 1917 Fragments arranged by size: 1917 1384 890 516 504 459 425 319 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6485 AflIII A'CryG_T Cuts at: 1203 5581 6558 1203 Size: 4378 977 1327 Fragments arranged by size: 4378 1327 977 AgeI A'CCGG_T Cuts at: 5513 5513 Size: 6682 AhdI GACnn_n'nnGTC Cuts at: 2868 5411 2868 Size: 2543 4139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6682 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3117 1184 Size: 1933 4749 AlwNI CAG_nnn'CTG Cuts at: 2957 3496 4425 5997 2957 Size: 539 929 1572 3642 Fragments arranged by size: 3642 1572 929 539 ApaLI G'TGCA_C Cuts at: 2105 4739 5895 2105 Size: 2634 1156 2892 Fragments arranged by size: 2892 2634 1156 ApoI r'AATT_y Cuts at: 1493 2277 2502 3326 3677 3771 3939 3991 4217 Size: 784 225 824 351 94 168 52 226 Cuts at: 4217 4369 6478 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 824 784 351 226 225 168 152 94 52 AseI AT'TA_AT Cuts at: 160 1032 5236 160 Size: 872 4204 1606 Fragments arranged by size: 4204 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 AvaI C'yCGr_G Cuts at: 1870 1943 4007 1870 Size: 73 2064 4545 Fragments arranged by size: 4545 2064 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2395 2428 2395 Size: 33 6649 BamHI G'GATC_C Cuts at: 4012 4379 4012 Size: 367 6315 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4003 5458 618 Size: 325 217 627 2216 1455 1842 Fragments arranged by size: 2216 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3078 4001 1391 Size: 1687 923 4072 Fragments arranged by size: 4072 1687 923 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2335 2558 2829 928 Size: 971 436 223 271 4781 Fragments arranged by size: 4781 971 436 271 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4873 4907 1903 Size: 34 2936 34 3678 Fragments arranged by size: 3678 2936 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3170 4574 5784 3170 Size: 1404 1210 4068 Fragments arranged by size: 4068 1404 1210 BclI T'GATC_A Cuts at: 1548 3297 1548 Size: 1749 4933 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5293 136 Size: 107 122 71 631 605 3621 1525 Fragments arranged by size: 3621 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2592 6677 2592 Size: 4085 2597 BlpI GC'TnA_GC Cuts at: 2877 4099 2877 Size: 1222 5460 Bme1580I G_kGCm'C Cuts at: 1790 2109 4743 5899 1790 Size: 319 2634 1156 2573 Fragments arranged by size: 2634 2573 1156 319 BmgBI CAC'GTC Cuts at: 1318 3283 1318 Size: 1965 4717 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4413 5371 442 Size: 416 53 83 399 24 2996 958 1753 Fragments arranged by size: 2996 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6682 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2211 2914 3560 5342 1239 Size: 803 169 703 646 1782 2579 Fragments arranged by size: 2579 1782 803 703 646 169 Bpu10I CC'TnA_GC Cuts at: 2616 3072 2616 Size: 456 6226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2970 4807 5672 5970 2970 Size: 1837 865 298 3682 Fragments arranged by size: 3682 1837 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3558 5345 882 Size: 617 2059 1787 2219 Fragments arranged by size: 2219 2059 1787 617 BsaAI yAC'GTr Cuts at: 493 1204 4473 493 Size: 711 3269 2702 Fragments arranged by size: 3269 2702 711 BsaBI GATnn'nnATC Cuts at: 3181 6676 3181 Size: 3495 3187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3390 4489 4871 5506 Size: 53 83 186 936 1857 1099 382 635 Cuts at: 5506 275 Size: 1451 Fragments arranged by size: 1857 1451 1099 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 2547 3687 3700 5115 5513 5787 5934 6345 Size: 147 1140 13 1415 398 274 147 411 Cuts at: 6345 2400 Size: 2737 Fragments arranged by size: 2737 1415 1140 411 398 274 147 147 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2099 2129 2330 2360 1178 Size: 30 165 30 696 30 201 30 5500 Fragments arranged by size: 5500 696 201 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 2597 1186 Size: 1028 383 5271 Fragments arranged by size: 5271 1028 383 BseYI C'CCAG_C Cuts at: 3226 5885 6381 3226 Size: 2659 496 3527 Fragments arranged by size: 3527 2659 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3379 3398 1163 Size: 573 1643 19 4447 Fragments arranged by size: 4447 1643 573 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4132 4893 5921 1540 Size: 275 186 2131 761 1028 2301 Fragments arranged by size: 2301 2131 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2109 2767 3078 4743 4828 5899 2109 Size: 658 311 1665 85 1071 2892 Fragments arranged by size: 2892 1665 1071 658 311 85 BsmI GAATG_Cn' Cuts at: 2695 3157 4193 4286 2695 Size: 462 1036 93 5091 Fragments arranged by size: 5091 1036 462 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3854 3854 Size: 6682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2767 3078 4001 4743 4828 5899 Size: 399 319 658 311 923 742 85 1071 Cuts at: 5899 1391 Size: 2174 Fragments arranged by size: 2174 1071 923 742 658 399 319 311 85 BspEI T'CCGG_A Cuts at: 6345 6345 Size: 6682 BspHI T'CATG_A Cuts at: 3348 4571 6301 3348 Size: 1223 1730 3729 Fragments arranged by size: 3729 1730 1223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4037 844 Size: 3193 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4569 1394 Size: 429 2746 3507 Fragments arranged by size: 3507 2746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3831 5177 5351 65 Size: 1183 2583 1346 174 1396 Fragments arranged by size: 2583 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5326 5513 1700 Size: 110 3516 187 2869 Fragments arranged by size: 3516 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3894 96 Size: 3798 2884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6682 BssSI C'ACGA_G Cuts at: 2766 4742 5754 2766 Size: 1976 1012 3694 Fragments arranged by size: 3694 1976 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6682 BstBI TT'CG_AA Cuts at: 5486 5486 Size: 6682 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6682 BstXI CCAn_nnnn'nTGG Cuts at: 2269 3690 2269 Size: 1421 5261 BstYI r'GATC_y Cuts at: 1972 2592 4012 4379 4764 4781 6233 6319 6331 Size: 620 1420 367 385 17 1452 86 12 Cuts at: 6331 6677 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1420 620 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2580 3461 2580 Size: 881 5801 BtgI C'CryG_G Cuts at: 513 1065 2004 2753 3528 513 Size: 552 939 749 775 3667 Fragments arranged by size: 3667 939 775 749 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5943 Fragments arranged by size: 5943 683 56 BtsI GCAGTG_nn' Cuts at: 2019 3162 4198 4992 5012 2019 Size: 1143 1036 794 20 3689 Fragments arranged by size: 3689 1143 1036 794 20 ClaI AT'CG_AT Cuts at: 1554 2448 1554 Size: 894 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6647 DraI TTT'AAA Cuts at: 3986 3986 Size: 6682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5689 809 Size: 507 4373 1802 Fragments arranged by size: 4373 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3285 3435 Size: 54 1425 50 275 18 177 1278 150 Cuts at: 3435 4129 5018 6647 8 Size: 694 889 1629 43 Fragments arranged by size: 1629 1425 1278 889 694 275 177 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4129 1537 Size: 275 2317 4090 Fragments arranged by size: 4090 2317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2143 2242 2250 2571 4612 1353 Size: 790 99 8 321 2041 3423 Fragments arranged by size: 3423 2041 790 321 99 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5269 5643 5789 387 Size: 1064 138 3680 374 146 1280 Fragments arranged by size: 3680 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3076 3076 Size: 6682 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2057 2211 2376 2835 2914 3339 Size: 393 410 15 154 165 459 79 425 Cuts at: 3339 3560 3855 4745 5342 6129 6397 1239 Size: 221 295 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 459 425 410 393 295 268 221 165 154 79 15 EcoO109I rG'GnC_Cy Cuts at: 2330 2980 3205 2330 Size: 650 225 5807 Fragments arranged by size: 5807 650 225 EcoRI G'AATT_C Cuts at: 3326 3991 4369 3326 Size: 665 378 5639 Fragments arranged by size: 5639 665 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2316 2316 Size: 6682 FspI TGC'GCA Cuts at: 5188 5188 Size: 6682 HaeII r_GCGC'y Cuts at: 1678 2089 3409 5829 1678 Size: 411 1320 2420 2531 Fragments arranged by size: 2531 2420 1320 411 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2854 2886 3962 3994 5323 Size: 32 135 32 1282 32 1076 32 1329 Cuts at: 5323 5355 5397 5429 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1329 1282 1076 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4026 4272 669 Size: 988 281 2088 246 3079 Fragments arranged by size: 3079 2088 988 281 246 HindIII A'AGCT_T Cuts at: 748 2524 748 Size: 1776 4906 HpaI GTT'AAC Cuts at: 4272 4272 Size: 6682 KpnI G_GTAC'C Cuts at: 4007 4007 Size: 6682 MfeI C'AATT_G Cuts at: 4281 4281 Size: 6682 MluI A'CGCG_T Cuts at: 6558 6558 Size: 6682 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5796 5980 1305 Size: 4491 184 2007 Fragments arranged by size: 4491 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3287 3437 10 Size: 54 1425 343 1455 150 3255 Fragments arranged by size: 3255 1455 1425 343 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 2834 3293 4640 4999 5158 518 Size: 648 1518 150 459 1347 359 159 2042 Fragments arranged by size: 2042 1518 1347 648 459 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2707 3496 4098 4775 5923 6168 1606 Size: 1101 789 602 677 1148 245 2120 Fragments arranged by size: 2120 1148 1101 789 677 602 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2753 3528 513 Size: 552 939 749 775 3667 Fragments arranged by size: 3667 939 775 749 552 NdeI CA'TA_TG Cuts at: 387 3796 387 Size: 3409 3273 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6682 NotI GC'GGCC_GC Cuts at: 4129 4129 Size: 6682 NspI r_CATG'y Cuts at: 1132 1229 2683 4040 5585 1132 Size: 97 1454 1357 1545 2229 Fragments arranged by size: 2229 1545 1454 1357 97 PasI CC'CwG_GG Cuts at: 1348 3781 1348 Size: 2433 4249 PciI A'CATG_T Cuts at: 5581 5581 Size: 6682 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 5284 PfoI T'CCnGG_A Cuts at: 3642 3642 Size: 6682 PmeI GTTT'AAAC Cuts at: 3986 3986 Size: 6682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4751 4783 6264 6296 4751 Size: 32 1481 32 5137 Fragments arranged by size: 5137 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2980 3205 2980 Size: 225 6457 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6682 PsiI TTA'TAA Cuts at: 4252 4252 Size: 6682 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2149 2181 3707 3739 2149 Size: 32 1526 32 5092 Fragments arranged by size: 5092 1526 32 32 PstI C_TGCA'G Cuts at: 830 3106 3169 4034 830 Size: 2276 63 865 3478 Fragments arranged by size: 3478 2276 865 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6682 PvuII CAG'CTG Cuts at: 2707 3496 2707 Size: 789 5893 SacI G_AGCT'C Cuts at: 3078 3078 Size: 6682 SalI G'TCGA_C Cuts at: 1936 4024 1936 Size: 2088 4594 SanDI GG'GwC_CC Cuts at: 2980 2980 Size: 6682 SbfI CC_TGCA'GG Cuts at: 4034 4034 Size: 6682 ScaI AGT'ACT Cuts at: 4930 6642 4930 Size: 1712 4970 SfcI C'TryA_G Cuts at: 826 1045 2702 3102 3165 3853 4030 5165 5846 Size: 219 1657 400 63 688 177 1135 681 Cuts at: 5846 6037 826 Size: 191 1471 Fragments arranged by size: 1657 1471 1135 688 681 400 219 191 177 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6682 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6682 SmaI CCC'GGG Cuts at: 1872 4009 1872 Size: 2137 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2985 4786 5687 5949 820 Size: 197 926 1042 1801 901 262 1553 Fragments arranged by size: 1801 1553 1042 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4473 493 Size: 3980 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6682 SphI G_CATG'C Cuts at: 1132 4040 1132 Size: 2908 3774 SspI AAT'ATT Cuts at: 5 52 2726 3955 4606 5 Size: 47 2674 1229 651 2081 Fragments arranged by size: 2674 2081 1229 651 47 StuI AGG'CCT Cuts at: 1517 3171 3843 3859 1517 Size: 1654 672 16 4340 Fragments arranged by size: 4340 1654 672 16 StyI C'CwwG_G Cuts at: 513 1065 2004 2753 3528 3838 4121 513 Size: 552 939 749 775 310 283 3074 Fragments arranged by size: 3074 939 775 749 552 310 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4709 4726 4879 5064 5509 325 Size: 1255 3129 17 153 185 445 1498 Fragments arranged by size: 3129 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3429 3894 4928 Size: 274 80 33 51 1443 1452 465 1034 Cuts at: 4928 6640 96 Size: 1712 138 Fragments arranged by size: 1712 1452 1443 1034 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2099 2131 3690 3722 6642 6674 2099 Size: 32 1559 32 2920 32 2107 Fragments arranged by size: 2920 2107 1559 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6682 XbaI T'CTAG_A Cuts at: 3958 4018 3958 Size: 60 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2488 3169 3535 3640 2488 Size: 681 366 105 5530 Fragments arranged by size: 5530 681 366 105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6682 XmaI C'CCGG_G Cuts at: 1870 4007 1870 Size: 2137 4545 XmnI GAAnn'nnTTC Cuts at: 4811 4811 Size: 6682 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4490 276 Size: 53 83 186 936 2956 2468 Fragments arranged by size: 2956 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI