Restriction map for
pFN21AE0996
(Linear) (Six-base) MAPSORT of: KIEE0996.seq Check: 9895 from: 1 to: 1977
KIEE0996.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 371 830 1334 1850 1977
Size: 52 319 459 504 516 127
Fragments arranged by size:
516 504 459 319 127 52
AhdI GACnn_n'nnGTC
Cuts at: 0 863 1977
Size: 863 1114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1112 1977
Size: 1112 865
AlwNI CAG_nnn'CTG
Cuts at: 0 952 1491 1977
Size: 952 539 486
Fragments arranged by size:
952 539 486
ApaLI G'TGCA_C
Cuts at: 0 100 1977
Size: 100 1877
ApoI r'AATT_y
Cuts at: 0 272 497 1321 1672 1766 1934 1977
Size: 272 225 824 351 94 168 43
Fragments arranged by size:
824 351 272 225 168 94 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 390 423 1977
Size: 390 33 1554
Fragments arranged by size:
1554 390 33
BanII G_rGCy'C
Cuts at: 0 1073 1977
Size: 1073 904
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 553 824 1977
Size: 330 223 271 1153
Fragments arranged by size:
1153 330 271 223
BciVI GTATCCnnnnn_n'
Cuts at: 0 1165 1977
Size: 1165 812
BclI T'GATC_A
Cuts at: 0 1292 1977
Size: 1292 685
BglII A'GATC_T
Cuts at: 0 587 1977
Size: 587 1390
BlpI GC'TnA_GC
Cuts at: 0 872 1977
Size: 872 1105
Bme1580I G_kGCm'C
Cuts at: 0 104 1977
Size: 104 1873
BmgBI CAC'GTC
Cuts at: 0 1278 1977
Size: 1278 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 206 909 1555 1977
Size: 37 169 703 646 422
Fragments arranged by size:
703 646 422 169 37
Bpu10I CC'TnA_GC
Cuts at: 0 611 1067 1977
Size: 611 456 910
Fragments arranged by size:
910 611 456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1977
Size: 965 1012
BsaI GGTCTCn'nnnn_
Cuts at: 0 1553 1977
Size: 1553 424
BsaBI GATnn'nnATC
Cuts at: 0 1176 1977
Size: 1176 801
BsaHI Gr'CG_yC
Cuts at: 0 1385 1977
Size: 1385 592
BsaWI w'CCGG_w
Cuts at: 0 395 542 1682 1695 1977
Size: 395 147 1140 13 282
Fragments arranged by size:
1140 395 282 147 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 325 355 1977
Size: 94 30 201 30 1622
Fragments arranged by size:
1622 201 94 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 592 1977
Size: 209 383 1385
Fragments arranged by size:
1385 383 209
BseYI C'CCAG_C
Cuts at: 0 1221 1977
Size: 1221 756
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1374 1393 1977
Size: 1374 19 584
Fragments arranged by size:
1374 584 19
BsiHKAI G_wGCw'C
Cuts at: 0 104 762 1073 1977
Size: 104 658 311 904
Fragments arranged by size:
904 658 311 104
BsmI GAATG_Cn'
Cuts at: 0 690 1152 1977
Size: 690 462 825
Fragments arranged by size:
825 690 462
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1849 1977
Size: 1849 128
Bsp1286I G_dGCh'C
Cuts at: 0 104 762 1073 1977
Size: 104 658 311 904
Fragments arranged by size:
904 658 311 104
BspHI T'CATG_A
Cuts at: 0 1343 1977
Size: 1343 634
BsrDI GCAATG_nn'
Cuts at: 0 1826 1977
Size: 1826 151
BsrGI T'GTAC_A
Cuts at: 0 1889 1977
Size: 1889 88
BssSI C'ACGA_G
Cuts at: 0 761 1977
Size: 761 1216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 1685 1977
Size: 264 1421 292
Fragments arranged by size:
1421 292 264
BstYI r'GATC_y
Cuts at: 0 587 1977
Size: 587 1390
Bsu36I CC'TnA_GG
Cuts at: 0 575 1456 1977
Size: 575 881 521
Fragments arranged by size:
881 575 521
BtgI C'CryG_G
Cuts at: 0 748 1523 1977
Size: 748 775 454
Fragments arranged by size:
775 748 454
BtsI GCAGTG_nn'
Cuts at: 0 14 1157 1977
Size: 14 1143 820
Fragments arranged by size:
1143 820 14
ClaI AT'CG_AT
Cuts at: 0 443 1977
Size: 443 1534
EaeI y'GGCC_r
Cuts at: 0 2 1280 1430 1977
Size: 2 1278 150 547
Fragments arranged by size:
1278 547 150 2
EarI CTCTTCn'nnn_
Cuts at: 0 138 237 245 566 1977
Size: 138 99 8 321 1411
Fragments arranged by size:
1411 321 138 99 8
EcoICRI GAG'CTC
Cuts at: 0 1071 1977
Size: 1071 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 52 206 371 830 909 1334 1555
Size: 37 15 154 165 459 79 425 221
Cuts at: 1555 1850 1977
Size: 295 127
Fragments arranged by size:
459 425 295 221 165 154 127 79
37 15
EcoO109I rG'GnC_Cy
Cuts at: 0 325 975 1200 1977
Size: 325 650 225 777
Fragments arranged by size:
777 650 325 225
EcoRI G'AATT_C
Cuts at: 0 1321 1977
Size: 1321 656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 311 1977
Size: 311 1666
HaeII r_GCGC'y
Cuts at: 0 84 1404 1977
Size: 84 1320 573
Fragments arranged by size:
1320 573 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 849 881 1957 1977
Size: 849 32 1076 20
Fragments arranged by size:
1076 849 32 20
HindIII A'AGCT_T
Cuts at: 0 519 1977
Size: 519 1458
MscI TGG'CCA
Cuts at: 0 1282 1432 1977
Size: 1282 150 545
Fragments arranged by size:
1282 545 150
MslI CAynn'nnrTG
Cuts at: 0 679 829 1288 1977
Size: 679 150 459 689
Fragments arranged by size:
689 679 459 150
MspA1I CmG'CkG
Cuts at: 0 702 1491 1977
Size: 702 789 486
Fragments arranged by size:
789 702 486
NcoI C'CATG_G
Cuts at: 0 748 1523 1977
Size: 748 775 454
Fragments arranged by size:
775 748 454
NdeI CA'TA_TG
Cuts at: 0 1791 1977
Size: 1791 186
NspI r_CATG'y
Cuts at: 0 678 1977
Size: 678 1299
PasI CC'CwG_GG
Cuts at: 0 1776 1977
Size: 1776 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 1977
Size: 501 1476
PfoI T'CCnGG_A
Cuts at: 0 1637 1977
Size: 1637 340
PpuMI rG'GwC_Cy
Cuts at: 0 975 1200 1977
Size: 975 225 777
Fragments arranged by size:
975 777 225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 144 176 1702 1734 1977
Size: 144 32 1526 32 243
Fragments arranged by size:
1526 243 144 32 32
PstI C_TGCA'G
Cuts at: 0 1101 1164 1977
Size: 1101 63 813
Fragments arranged by size:
1101 813 63
PvuII CAG'CTG
Cuts at: 0 702 1491 1977
Size: 702 789 486
Fragments arranged by size:
789 702 486
SacI G_AGCT'C
Cuts at: 0 1073 1977
Size: 1073 904
SanDI GG'GwC_CC
Cuts at: 0 975 1977
Size: 975 1002
SfcI C'TryA_G
Cuts at: 0 697 1097 1160 1848 1977
Size: 697 400 63 688 129
Fragments arranged by size:
697 688 400 129 63
SmlI C'TyrA_G
Cuts at: 0 980 1977
Size: 980 997
SspI AAT'ATT
Cuts at: 0 721 1950 1977
Size: 721 1229 27
Fragments arranged by size:
1229 721 27
StuI AGG'CCT
Cuts at: 0 1166 1838 1854 1977
Size: 1166 672 16 123
Fragments arranged by size:
1166 672 123 16
StyI C'CwwG_G
Cuts at: 0 748 1523 1833 1977
Size: 748 775 310 144
Fragments arranged by size:
775 748 310 144
TatI w'GTAC_w
Cuts at: 0 1424 1889 1977
Size: 1424 465 88
Fragments arranged by size:
1424 465 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 1685 1717 1977
Size: 94 32 1559 32 260
Fragments arranged by size:
1559 260 94 32 32
XbaI T'CTAG_A
Cuts at: 0 1953 1977
Size: 1953 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 1164 1530 1635 1977
Size: 483 681 366 105 342
Fragments arranged by size:
681 483 366 342 105
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApaLI ApoI BaeI BanII
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI
BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HindIII MscI MslI MspA1I NcoI NdeI NspI
PasI PflMI PfoI PpuMI PsrI PstI PvuII SacI
SanDI SfcI SmlI SspI StuI StyI TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BmrI BmtI BplI BsaAI BsiEI BsiWI BspEI BspMI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XhoI XmaI
XmnI ZraI