(Circular) (Six-base) MAPSORT of: pFN21AB7347.seq Check: 1739 from: 1 to: 8014 pFN21AB7347 8014 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3098 3098 Size: 8014 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4191 5824 278 Size: 53 83 186 936 2655 1633 2468 Fragments arranged by size: 2655 2468 1633 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4767 5357 1937 Size: 2830 590 4594 Fragments arranged by size: 4594 2830 590 Acc65I G'GTAC_C Cuts at: 3296 5335 3296 Size: 2039 5975 AclI AA'CG_TT Cuts at: 1563 6141 6514 1563 Size: 4578 373 3063 Fragments arranged by size: 4578 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2976 3710 3869 4025 5159 6077 7461 7729 Size: 1344 734 159 156 1134 918 1384 268 Cuts at: 7729 1632 Size: 1917 Fragments arranged by size: 1917 1384 1344 1134 918 734 268 159 156 AfeI AGC'GCT Cuts at: 2301 2774 2301 Size: 473 7541 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7817 AflIII A'CryG_T Cuts at: 1203 4857 6913 7890 1203 Size: 3654 2056 977 1327 Fragments arranged by size: 3654 2056 1327 977 AgeI A'CCGG_T Cuts at: 6845 6845 Size: 8014 AhdI GACnn_n'nnGTC Cuts at: 6743 6743 Size: 8014 AleI CACnn'nnGTG Cuts at: 1166 2726 1166 Size: 1560 6454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4955 5267 1184 Size: 3771 312 3931 Fragments arranged by size: 3931 3771 312 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3911 3943 3911 Size: 32 7982 AlwNI CAG_nnn'CTG Cuts at: 2581 4573 5757 7329 2581 Size: 1992 1184 1572 3266 Fragments arranged by size: 3266 1992 1572 1184 ApaI G_GGCC'C Cuts at: 2101 2186 2626 2715 2917 2101 Size: 85 440 89 202 7198 Fragments arranged by size: 7198 440 202 89 85 ApaLI G'TGCA_C Cuts at: 6071 7227 6071 Size: 1156 6858 ApoI r'AATT_y Cuts at: 1493 3695 5323 5549 5701 7810 1493 Size: 2202 1628 226 152 2109 1697 Fragments arranged by size: 2202 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 6568 160 Size: 872 5536 1606 Fragments arranged by size: 5536 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8014 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2094 2211 2309 2625 2682 3273 Size: 73 102 49 117 98 316 57 591 Cuts at: 3273 3807 4262 4622 4807 4945 5101 5267 5339 Size: 534 455 360 185 138 156 166 72 Cuts at: 5339 1870 Size: 4545 Fragments arranged by size: 4545 591 534 455 360 316 185 166 156 138 117 102 98 73 72 57 49 AvrII C'CTAG_G Cuts at: 3184 3184 Size: 8014 BamHI G'GATC_C Cuts at: 3265 5344 5711 3265 Size: 2079 367 5568 Fragments arranged by size: 5568 2079 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2446 2480 3047 3296 3468 Size: 325 217 627 659 34 567 249 172 Cuts at: 3468 3557 3599 4330 5150 5335 6790 618 Size: 89 42 731 820 185 1455 1842 Fragments arranged by size: 1842 1455 820 731 659 627 567 325 249 217 185 172 89 42 34 BanII G_rGCy'C Cuts at: 1391 2052 2074 2101 2186 2218 2333 2626 2664 Size: 661 22 27 85 32 115 293 38 Cuts at: 2664 2683 2715 2917 2989 3204 3281 3765 3881 Size: 19 32 202 72 215 77 484 116 Cuts at: 3881 4257 4292 4623 4698 5333 1391 Size: 376 35 331 75 635 4072 Fragments arranged by size: 4072 661 635 484 376 331 293 215 202 116 115 85 77 75 72 38 35 32 32 27 22 19 BbeI G_GCGC'C Cuts at: 5154 5154 Size: 8014 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2818 3058 4448 4553 4607 928 Size: 971 919 240 1390 105 54 4335 Fragments arranged by size: 4335 1390 971 919 240 105 54 BbvCI CC'TCA_GC Cuts at: 4590 4590 Size: 8014 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6205 6239 1903 Size: 34 4268 34 3678 Fragments arranged by size: 4268 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4355 5906 7116 4355 Size: 1551 1210 5253 Fragments arranged by size: 5253 1551 1210 BclI T'GATC_A Cuts at: 1548 2730 1548 Size: 1182 6832 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 2277 2687 Size: 107 122 71 631 605 568 37 410 Cuts at: 2687 3452 6625 136 Size: 765 3173 1525 Fragments arranged by size: 3173 1525 765 631 605 568 410 122 107 71 37 BglII A'GATC_T Cuts at: 8009 8009 Size: 8014 BlpI GC'TnA_GC Cuts at: 5431 5431 Size: 8014 Bme1580I G_kGCm'C Cuts at: 1790 2101 2186 2451 2483 2626 2715 2917 3560 Size: 311 85 265 32 143 89 202 643 Cuts at: 3560 3604 4237 4889 6075 7231 1790 Size: 44 633 652 1186 1156 2573 Fragments arranged by size: 2573 1186 1156 652 643 633 311 265 202 143 89 85 44 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8014 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5745 6703 442 Size: 416 53 83 399 24 4328 958 1753 Fragments arranged by size: 4328 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8014 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3025 4311 4501 3025 Size: 1286 190 6538 Fragments arranged by size: 6538 1286 190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2697 2804 3407 3740 4181 4271 4496 4517 Size: 1458 107 603 333 441 90 225 21 Cuts at: 4517 4550 5306 6674 1239 Size: 33 756 1368 2579 Fragments arranged by size: 2579 1458 1368 756 603 441 333 225 107 90 33 21 Bpu10I CC'TnA_GC Cuts at: 4145 4590 4145 Size: 445 7569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2973 4028 5089 6139 7004 7302 2973 Size: 1055 1061 1050 865 298 3685 Fragments arranged by size: 3685 1061 1055 1050 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3037 3231 3499 3862 6677 882 Size: 617 1538 194 268 363 2815 2219 Fragments arranged by size: 2815 2219 1538 617 363 268 194 BsaAI yAC'GTr Cuts at: 493 1204 5062 5805 493 Size: 711 3858 743 2702 Fragments arranged by size: 3858 2702 743 711 BsaBI GATnn'nnATC Cuts at: 8008 8008 Size: 8014 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2324 4188 4791 5151 Size: 53 83 186 936 791 1864 603 360 Cuts at: 5151 5821 6203 6838 275 Size: 670 382 635 1451 Fragments arranged by size: 1864 1451 936 791 670 635 603 382 360 186 83 53 BsaWI w'CCGG_w Cuts at: 2038 2383 3125 4096 6447 6845 7119 7266 7677 Size: 345 742 971 2351 398 274 147 411 Cuts at: 7677 2038 Size: 2375 Fragments arranged by size: 2375 2351 971 742 411 398 345 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3120 3150 3666 3696 4610 Size: 30 165 30 1717 30 516 30 914 Cuts at: 4610 4640 1178 Size: 30 4552 Fragments arranged by size: 4552 1717 914 516 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 2810 3204 3212 3884 4538 1186 Size: 1594 30 394 8 672 654 4662 Fragments arranged by size: 4662 1594 672 654 394 30 8 BseYI C'CCAG_C Cuts at: 2335 2544 2755 2800 2901 3440 3632 3727 4153 Size: 209 211 45 101 539 192 95 426 Cuts at: 4153 4365 4818 7217 7713 2335 Size: 212 453 2399 496 2636 Fragments arranged by size: 2636 2399 539 496 453 426 212 211 209 192 101 95 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3853 3959 4295 4925 1163 Size: 573 2117 106 336 630 4252 Fragments arranged by size: 4252 2117 630 573 336 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5300 5464 6225 7253 1540 Size: 275 186 3299 164 761 1028 2301 Fragments arranged by size: 3299 2301 1028 761 275 186 164 BsiHKAI G_wGCw'C Cuts at: 2931 3765 3867 4257 5075 5095 6075 6160 7231 Size: 834 102 390 818 20 980 85 1071 Cuts at: 7231 2931 Size: 3714 Fragments arranged by size: 3714 1071 980 834 818 390 102 85 20 BsmI GAATG_Cn' Cuts at: 2537 5525 5618 2537 Size: 2988 93 4933 Fragments arranged by size: 4933 2988 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3186 3186 Size: 8014 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2074 2101 2186 2218 2333 2451 Size: 399 262 22 27 85 32 115 118 Cuts at: 2451 2483 2626 2664 2683 2715 2917 2931 2989 Size: 32 143 38 19 32 202 14 58 Cuts at: 2989 3204 3281 3560 3604 3765 3867 3881 4237 Size: 215 77 279 44 161 102 14 356 Cuts at: 4237 4257 4292 4623 4698 4889 5075 5095 5333 Size: 20 35 331 75 191 186 20 238 Cuts at: 5333 6075 6160 7231 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 356 331 279 262 238 215 202 191 186 161 143 118 115 102 85 85 77 75 58 44 38 35 32 32 32 27 22 20 20 19 14 14 BspEI T'CCGG_A Cuts at: 2038 3125 7677 2038 Size: 1087 4552 2375 Fragments arranged by size: 4552 2375 1087 BspHI T'CATG_A Cuts at: 5903 7633 5903 Size: 1730 6284 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3019 3098 3151 4722 5226 5369 844 Size: 2175 79 53 1571 504 143 3489 Fragments arranged by size: 3489 2175 1571 504 143 79 53 BsrBI CCG'CTC Cuts at: 1394 1823 2027 2116 4112 5272 5901 1394 Size: 429 204 89 1996 1160 629 3507 Fragments arranged by size: 3507 1996 1160 629 429 204 89 BsrDI GCAATG_nn' Cuts at: 65 1248 6509 6683 65 Size: 1183 5261 174 1396 Fragments arranged by size: 5261 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2403 3240 3293 3471 4181 4554 6658 Size: 110 593 837 53 178 710 373 2104 Cuts at: 6658 6845 1700 Size: 187 2869 Fragments arranged by size: 2869 2104 837 710 593 373 187 178 110 53 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8014 BssHII G'CGCG_C Cuts at: 1925 2549 1925 Size: 624 7390 BssSI C'ACGA_G Cuts at: 2375 3346 6074 7086 2375 Size: 971 2728 1012 3303 Fragments arranged by size: 3303 2728 1012 971 BstAPI GCAn_nnn'nTGC Cuts at: 794 2749 4282 794 Size: 1955 1533 4526 Fragments arranged by size: 4526 1955 1533 BstBI TT'CG_AA Cuts at: 6818 6818 Size: 8014 BstEII G'GTnAC_C Cuts at: 1184 2563 1184 Size: 1379 6635 BstXI CCAn_nnnn'nTGG Cuts at: 3551 4826 3551 Size: 1275 6739 BstYI r'GATC_y Cuts at: 1972 3265 4501 5259 5344 5711 6096 6113 7565 Size: 1293 1236 758 85 367 385 17 1452 Cuts at: 7565 7651 7663 8009 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1293 1236 758 385 367 346 86 85 17 12 Bsu36I CC'TnA_GG Cuts at: 2693 4911 2693 Size: 2218 5796 BtgI C'CryG_G Cuts at: 513 1065 2484 2508 2610 2655 3522 4057 4063 Size: 552 1419 24 102 45 867 535 6 Cuts at: 4063 4320 4395 4881 4985 5239 513 Size: 257 75 486 104 254 3288 Fragments arranged by size: 3288 1419 867 552 535 486 257 254 104 102 75 45 24 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7275 Fragments arranged by size: 7275 683 56 BtsI GCAGTG_nn' Cuts at: 2733 3865 4237 4367 5530 6324 6344 2733 Size: 1132 372 130 1163 794 20 4403 Fragments arranged by size: 4403 1163 1132 794 372 130 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3066 3101 529 Size: 35 2502 35 5442 Fragments arranged by size: 5442 2502 35 35 DraI TTT'AAA Cuts at: 5318 5318 Size: 8014 DraIII CAC_nnn'GTG Cuts at: 2749 2749 Size: 8014 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7021 809 Size: 507 5705 1802 Fragments arranged by size: 5705 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2229 2241 2405 Size: 54 1425 50 275 18 399 12 164 Cuts at: 2405 2880 4060 4179 4200 5297 5461 6350 7979 Size: 475 1180 119 21 1097 164 889 1629 Cuts at: 7979 8 Size: 43 Fragments arranged by size: 1629 1425 1180 1097 889 475 399 275 164 164 119 54 50 43 21 18 12 EagI C'GGCC_G Cuts at: 1537 1812 5297 5461 1537 Size: 275 3485 164 4090 Fragments arranged by size: 4090 3485 275 164 EarI CTCTTCn'nnn_ Cuts at: 1353 3888 5944 1353 Size: 2535 2056 3423 Fragments arranged by size: 3423 2535 2056 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2146 2489 3280 3713 6601 6975 Size: 1064 138 557 343 791 433 2888 374 Cuts at: 6975 7121 387 Size: 146 1280 Fragments arranged by size: 2888 1280 1064 791 557 433 374 343 146 138 EcoICRI GAG'CTC Cuts at: 3763 4255 3763 Size: 492 7522 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 2804 2976 3407 3710 3740 3869 Size: 393 1065 107 172 431 303 30 129 Cuts at: 3869 4025 4181 4271 4496 4517 4550 5159 5306 Size: 156 156 90 225 21 33 609 147 Cuts at: 5306 6077 6674 7461 7729 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 1065 787 771 609 597 431 393 303 268 225 172 156 156 147 129 107 90 33 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2696 2783 3836 3956 2696 Size: 87 1053 120 6754 Fragments arranged by size: 6754 1053 120 87 EcoO109I rG'GnC_Cy Cuts at: 2098 2176 2183 2578 2622 2689 2913 3163 3181 Size: 78 7 395 44 67 224 250 18 Cuts at: 3181 3444 3821 3889 4150 2098 Size: 263 377 68 261 5962 Fragments arranged by size: 5962 395 377 263 261 250 224 78 68 67 44 18 7 EcoRI G'AATT_C Cuts at: 5323 5701 5323 Size: 378 7636 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3760 3760 Size: 8014 FspI TGC'GCA Cuts at: 2981 3159 4925 6520 2981 Size: 178 1766 1595 4475 Fragments arranged by size: 4475 1766 1595 178 FspAI rTGC'GCAy Cuts at: 4925 4925 Size: 8014 HaeII r_GCGC'y Cuts at: 1678 2303 2776 2998 4168 5154 7161 1678 Size: 625 473 222 1170 986 2007 2531 Fragments arranged by size: 2531 2007 1170 986 625 473 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4026 4058 4561 4593 6655 Size: 32 135 32 2454 32 503 32 2062 Cuts at: 6655 6687 6729 6761 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2454 2062 503 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5014 5358 5604 669 Size: 988 281 3076 344 246 3079 Fragments arranged by size: 3079 3076 988 344 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8014 HpaI GTT'AAC Cuts at: 5604 5604 Size: 8014 KasI G'GCGC_C Cuts at: 5150 5150 Size: 8014 KpnI G_GTAC'C Cuts at: 3300 5339 3300 Size: 2039 5975 MfeI C'AATT_G Cuts at: 5613 5613 Size: 8014 MluI A'CGCG_T Cuts at: 7890 7890 Size: 8014 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7128 7312 1305 Size: 5823 184 2007 Fragments arranged by size: 5823 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4202 10 Size: 54 1425 343 2370 3822 Fragments arranged by size: 3822 2370 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2726 2738 3257 3488 3549 4208 5060 Size: 648 1560 12 519 231 61 659 852 Cuts at: 5060 5972 6331 6490 518 Size: 912 359 159 2042 Fragments arranged by size: 2042 1560 912 852 659 648 519 359 231 159 61 12 MspA1I CmG'CkG Cuts at: 1606 2510 3134 3627 4123 4822 4987 5430 6107 Size: 904 624 493 496 699 165 443 677 Cuts at: 6107 7255 7500 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 904 699 677 624 496 493 443 245 165 NaeI GCC'GGC Cuts at: 2405 4183 2405 Size: 1778 6236 NarI GG'CG_CC Cuts at: 5151 5151 Size: 8014 NcoI C'CATG_G Cuts at: 513 1065 2655 4320 4395 513 Size: 552 1590 1665 75 4132 Fragments arranged by size: 4132 1665 1590 552 75 NdeI CA'TA_TG Cuts at: 387 4905 387 Size: 4518 3496 NgoMIV G'CCGG_C Cuts at: 2403 4181 2403 Size: 1778 6236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8014 NotI GC'GGCC_GC Cuts at: 5461 5461 Size: 8014 NspI r_CATG'y Cuts at: 1132 1229 3262 3487 3613 4861 4900 5372 6917 Size: 97 2033 225 126 1248 39 472 1545 Cuts at: 6917 1132 Size: 2229 Fragments arranged by size: 2229 2033 1545 1248 472 225 126 97 39 PasI CC'CwG_GG Cuts at: 1348 2187 2451 2715 3577 3701 1348 Size: 839 264 264 862 124 5661 Fragments arranged by size: 5661 862 839 264 264 124 PciI A'CATG_T Cuts at: 4857 6913 4857 Size: 2056 5958 PflMI CCAn_nnn'nTGG Cuts at: 1108 3044 3643 1108 Size: 1936 599 5479 Fragments arranged by size: 5479 1936 599 PfoI T'CCnGG_A Cuts at: 2781 3150 3756 4509 2781 Size: 369 606 753 6286 Fragments arranged by size: 6286 753 606 369 PmeI GTTT'AAAC Cuts at: 5318 5318 Size: 8014 PmlI CAC'GTG Cuts at: 5062 5062 Size: 8014 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6083 6115 7596 7628 6083 Size: 32 1481 32 6469 Fragments arranged by size: 6469 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2578 3163 3181 3889 4150 2176 Size: 402 585 18 708 261 6040 Fragments arranged by size: 6040 708 585 402 261 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8014 PsiI TTA'TAA Cuts at: 5584 5584 Size: 8014 PspOMI G'GGCC_C Cuts at: 2097 2182 2622 2711 2913 2097 Size: 85 440 89 202 7198 Fragments arranged by size: 7198 440 202 89 85 PstI C_TGCA'G Cuts at: 830 2142 3027 5256 5366 830 Size: 1312 885 2229 110 3478 Fragments arranged by size: 3478 2229 1312 885 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8014 PvuII CAG'CTG Cuts at: 3134 4123 3134 Size: 989 7025 SacI G_AGCT'C Cuts at: 3765 4257 3765 Size: 492 7522 SacII CC_GC'GG Cuts at: 2511 4988 2511 Size: 2477 5537 SalI G'TCGA_C Cuts at: 1936 5356 1936 Size: 3420 4594 SanDI GG'GwC_CC Cuts at: 4150 4150 Size: 8014 SbfI CC_TGCA'GG Cuts at: 5256 5366 5256 Size: 110 7904 ScaI AGT'ACT Cuts at: 4484 4628 6262 7974 4484 Size: 144 1634 1712 4524 Fragments arranged by size: 4524 1712 1634 144 SexAI A'CCwGG_T Cuts at: 5109 5109 Size: 8014 SfcI C'TryA_G Cuts at: 826 1045 2138 3023 5252 5362 6497 7178 7369 Size: 219 1093 885 2229 110 1135 681 191 Cuts at: 7369 826 Size: 1471 Fragments arranged by size: 2229 1471 1135 1093 885 681 219 191 110 SfiI GGCCn_nnn'nGGCC Cuts at: 3452 3452 Size: 8014 SfoI GGC'GCC Cuts at: 5152 5152 Size: 8014 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8014 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8014 SmaI CCC'GGG Cuts at: 1872 2047 2096 2627 2684 3275 5341 1872 Size: 175 49 531 57 591 2066 4545 Fragments arranged by size: 4545 2066 591 531 175 57 49 SmlI C'TyrA_G Cuts at: 820 1017 1943 2988 4043 5068 6118 7019 7281 Size: 197 926 1045 1055 1025 1050 901 262 Cuts at: 7281 820 Size: 1553 Fragments arranged by size: 1553 1055 1050 1045 1025 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5805 493 Size: 5312 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8014 SphI G_CATG'C Cuts at: 1132 4900 5372 1132 Size: 3768 472 3774 Fragments arranged by size: 3774 3768 472 SspI AAT'ATT Cuts at: 5 52 5938 5 Size: 47 5886 2081 Fragments arranged by size: 5886 2081 47 StuI AGG'CCT Cuts at: 1517 2876 4595 4938 1517 Size: 1359 1719 343 4593 Fragments arranged by size: 4593 1719 1359 343 StyI C'CwwG_G Cuts at: 513 1065 2655 2664 2877 3184 4311 4320 4395 Size: 552 1590 9 213 307 1127 9 75 Cuts at: 4395 5453 513 Size: 1058 3074 Fragments arranged by size: 3074 1590 1127 1058 552 307 213 75 9 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2575 2747 2825 3109 3561 3587 6041 Size: 1255 995 172 78 284 452 26 2454 Cuts at: 6041 6058 6211 6396 6841 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2454 1498 1255 995 452 445 284 185 172 153 78 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4482 4626 4647 Size: 274 80 33 51 1443 2505 144 21 Cuts at: 4647 6260 7972 96 Size: 1613 1712 138 Fragments arranged by size: 2505 1712 1613 1443 274 144 138 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3120 3152 4109 4141 4854 4886 5074 5106 7974 Size: 32 957 32 713 32 188 32 2868 Cuts at: 7974 8006 3120 Size: 32 3128 Fragments arranged by size: 3128 2868 957 713 188 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3062 5009 1316 Size: 1746 1947 4321 Fragments arranged by size: 4321 1947 1746 XbaI T'CTAG_A Cuts at: 5350 5350 Size: 8014 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2662 3259 3640 4705 4861 2662 Size: 597 381 1065 156 5815 Fragments arranged by size: 5815 1065 597 381 156 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8014 XmaI C'CCGG_G Cuts at: 1870 2045 2094 2625 2682 3273 5339 1870 Size: 175 49 531 57 591 2066 4545 Fragments arranged by size: 4545 2066 591 531 175 57 49 XmnI GAAnn'nnTTC Cuts at: 3695 6143 3695 Size: 2448 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4189 5822 276 Size: 53 83 186 936 2655 1633 2468 Fragments arranged by size: 2655 2468 1633 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI