Restriction map for
pFN21AB7292
(Linear) (Six-base) MAPSORT of: KIBB7292.seq Check: 9197 from: 1 to: 3582
KIBB7292.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1690 3230 3582
Size: 1690 1540 352
Fragments arranged by size:
1690 1540 352
AccI GT'mk_AC
Cuts at: 0 2225 3582
Size: 2225 1357
AclI AA'CG_TT
Cuts at: 0 660 3582
Size: 660 2922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1481 3105 3582
Size: 1481 1624 477
Fragments arranged by size:
1624 1481 477
AfeI AGC'GCT
Cuts at: 0 106 3582
Size: 106 3476
AflIII A'CryG_T
Cuts at: 0 251 3326 3582
Size: 251 3075 256
Fragments arranged by size:
3075 256 251
AgeI A'CCGG_T
Cuts at: 0 413 3582
Size: 413 3169
AhdI GACnn_n'nnGTC
Cuts at: 0 432 3582
Size: 432 3150
AleI CACnn'nnGTG
Cuts at: 0 1861 3582
Size: 1861 1721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 901 1363 3496 3582
Size: 901 462 2133 86
Fragments arranged by size:
2133 901 462 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 3008 3040 3582
Size: 229 32 2747 32 542
Fragments arranged by size:
2747 542 229 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 519 3222 3231 3582
Size: 519 2703 9 351
Fragments arranged by size:
2703 519 351 9
ApaLI G'TGCA_C
Cuts at: 0 1483 2743 2932 3582
Size: 1483 1260 189 650
Fragments arranged by size:
1483 1260 650 189
AvaI C'yCGr_G
Cuts at: 0 187 3172 3468 3582
Size: 187 2985 296 114
Fragments arranged by size:
2985 296 187 114
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2196 2229 3108 3141 3582
Size: 2196 33 879 33 441
Fragments arranged by size:
2196 879 441 33 33
BanI G'GyrC_C
Cuts at: 0 911 2706 2729 3480 3582
Size: 911 1795 23 751 102
Fragments arranged by size:
1795 911 751 102 23
BanII G_rGCy'C
Cuts at: 0 11 147 1739 1889 2115 3582
Size: 11 136 1592 150 226 1467
Fragments arranged by size:
1592 1467 226 150 136 11
BbeI G_GCGC'C
Cuts at: 0 915 3582
Size: 915 2667
BbsI GAAGACnn'nnnn_
Cuts at: 0 2758 3368 3582
Size: 2758 610 214
Fragments arranged by size:
2758 610 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 77 111 1005 1039 3582
Size: 77 34 894 34 2543
Fragments arranged by size:
2543 894 77 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3540 3582
Size: 3540 42
BclI T'GATC_A
Cuts at: 0 2120 2171 3582
Size: 2120 51 1411
Fragments arranged by size:
2120 1411 51
BfrBI ATG'CAT
Cuts at: 0 2574 3582
Size: 2574 1008
BglI GCCn_nnn'nGGC
Cuts at: 0 1307 3582
Size: 1307 2275
BglII A'GATC_T
Cuts at: 0 1719 2075 3582
Size: 1719 356 1507
Fragments arranged by size:
1719 1507 356
BlpI GC'TnA_GC
Cuts at: 0 3244 3582
Size: 3244 338
Bme1580I G_kGCm'C
Cuts at: 0 188 1487 1933 2333 2408 2711 2747 2936
Size: 188 1299 446 400 75 303 36 189
Cuts at: 2936 3485 3582
Size: 549 97
Fragments arranged by size:
1299 549 446 400 303 189 188 97
75 36
BmtI G_CTAG'C
Cuts at: 0 2398 3582
Size: 2398 1184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3285 3582
Size: 3285 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 508 1016 1409 1860 1960 3034 3085
Size: 474 34 508 393 451 100 1074 51
Cuts at: 3085 3301 3582
Size: 216 281
Fragments arranged by size:
1074 508 474 451 393 281 216 100
51 34
Bpu10I CC'TnA_GC
Cuts at: 0 898 2114 3582
Size: 898 1216 1468
Fragments arranged by size:
1468 1216 898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1777 3582
Size: 1777 1805
BsaI GGTCTCn'nnnn_
Cuts at: 0 2989 3273 3582
Size: 2989 284 309
Fragments arranged by size:
2989 309 284
BsaAI yAC'GTr
Cuts at: 0 1305 2748 3327 3582
Size: 1305 1443 579 255
Fragments arranged by size:
1443 1305 579 255
BsaBI GATnn'nnATC
Cuts at: 0 2506 3582
Size: 2506 1076
BsaHI Gr'CG_yC
Cuts at: 0 912 920 3436 3582
Size: 912 8 2516 146
Fragments arranged by size:
2516 912 146 8
BsaWI w'CCGG_w
Cuts at: 0 413 3582
Size: 413 3169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 1382 1387 1412 1417 2690 2720
Size: 229 30 1123 5 25 5 1273 30
Cuts at: 2720 3008 3038 3313 3343 3582
Size: 288 30 275 30 239
Fragments arranged by size:
1273 1123 288 275 239 229 30 30
30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1408 1989 2275 2887 2890 2893 3007 3035
Size: 1408 581 286 612 3 3 114 28
Cuts at: 3035 3274 3304 3391 3394 3397 3582
Size: 239 30 87 3 3 185
Fragments arranged by size:
1408 612 581 286 239 185 114 87
30 28 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 27 793 2888 3484 3582
Size: 27 766 2095 596 98
Fragments arranged by size:
2095 766 596 98 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1164 1590 2064 3498 3582
Size: 532 632 426 474 1434 84
Fragments arranged by size:
1434 632 532 474 426 84
BsiEI CG_ry'CG
Cuts at: 0 176 3582
Size: 176 3406
BsiHKAI G_wGCw'C
Cuts at: 0 1487 1889 2747 2936 3250 3338 3512 3582
Size: 1487 402 858 189 314 88 174 70
Fragments arranged by size:
1487 858 402 314 189 174 88 70
BsmI GAATG_Cn'
Cuts at: 0 2658 3582
Size: 2658 924
BsmBI CGTCTCn'nnnn_
Cuts at: 0 104 1412 2755 3582
Size: 104 1308 1343 827
Fragments arranged by size:
1343 1308 827 104
Bsp1286I G_dGCh'C
Cuts at: 0 11 147 188 1487 1739 1889 1933 2115
Size: 11 136 41 1299 252 150 44 182
Cuts at: 2115 2333 2408 2711 2747 2936 3250 3338 3485
Size: 218 75 303 36 189 314 88 147
Cuts at: 3485 3512 3582
Size: 27 70
Fragments arranged by size:
1299 314 303 252 218 189 182 150
147 136 88 75 70 44 41 36
27 11
BspHI T'CATG_A
Cuts at: 0 1442 2099 2291 3582
Size: 1442 657 192 1291
Fragments arranged by size:
1442 1291 657 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 527 1690 3230 3398 3582
Size: 527 1163 1540 168 184
Fragments arranged by size:
1540 1163 527 184 168
BsrBI CCG'CTC
Cuts at: 0 716 2603 3342 3582
Size: 716 1887 739 240
Fragments arranged by size:
1887 739 716 240
BsrDI GCAATG_nn'
Cuts at: 0 464 1194 1943 2123 3582
Size: 464 730 749 180 1459
Fragments arranged by size:
1459 749 730 464 180
BsrFI r'CCGG_y
Cuts at: 0 413 3582
Size: 413 3169
BsrGI T'GTAC_A
Cuts at: 0 590 2372 2504 3582
Size: 590 1782 132 1078
Fragments arranged by size:
1782 1078 590 132
BssSI C'ACGA_G
Cuts at: 0 1086 3337 3582
Size: 1086 2251 245
Fragments arranged by size:
2251 1086 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 1278 1646 2653 3222 3231 3582
Size: 519 759 368 1007 569 9 351
Fragments arranged by size:
1007 759 569 519 368 351 9
BstEII G'GTnAC_C
Cuts at: 0 2163 3582
Size: 2163 1419
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2250 2818 3582
Size: 2250 568 764
Fragments arranged by size:
2250 764 568
BstYI r'GATC_y
Cuts at: 0 311 1719 2075 3582
Size: 311 1408 356 1507
Fragments arranged by size:
1507 1408 356 311
Bsu36I CC'TnA_GG
Cuts at: 0 604 3582
Size: 604 2978
BtgI C'CryG_G
Cuts at: 0 338 1256 2924 3278 3582
Size: 338 918 1668 354 304
Fragments arranged by size:
1668 918 354 338 304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1331 1773 2020 2597 3582
Size: 1331 442 247 577 985
Fragments arranged by size:
1331 985 577 442 247
BtsI GCAGTG_nn'
Cuts at: 0 312 1578 2459 2547 3582
Size: 312 1266 881 88 1035
Fragments arranged by size:
1266 1035 881 312 88
DraI TTT'AAA
Cuts at: 0 1170 3582
Size: 1170 2412
DraIII CAC_nnn'GTG
Cuts at: 0 3150 3582
Size: 3150 432
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 3441 3582
Size: 130 3311 141
Fragments arranged by size:
3311 141 130
EaeI y'GGCC_r
Cuts at: 0 113 173 429 1299 3011 3398 3582
Size: 113 60 256 870 1712 387 184
Fragments arranged by size:
1712 870 387 256 184 113 60
EagI C'GGCC_G
Cuts at: 0 173 3582
Size: 173 3409
EarI CTCTTCn'nnn_
Cuts at: 0 154 339 535 796 3010 3368 3576 3582
Size: 154 185 196 261 2214 358 208 6
Fragments arranged by size:
2214 358 261 208 196 185 154 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2954 3582
Size: 2954 628
EcoICRI GAG'CTC
Cuts at: 0 1887 3582
Size: 1887 1695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 508 1016 1409 1481 1860 1960 3034
Size: 474 34 508 393 72 379 100 1074
Cuts at: 3034 3085 3105 3301 3582
Size: 51 20 196 281
Fragments arranged by size:
1074 508 474 393 379 281 196 100
72 51 34 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3028 3582
Size: 3028 554
EcoO109I rG'GnC_Cy
Cuts at: 0 3160 3387 3582
Size: 3160 227 195
Fragments arranged by size:
3160 227 195
EcoRV GAT'ATC
Cuts at: 0 1440 3582
Size: 1440 2142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2245 3582
Size: 2245 1337
FspI TGC'GCA
Cuts at: 0 823 3582
Size: 823 2759
HaeII r_GCGC'y
Cuts at: 0 108 905 915 1983 3582
Size: 108 797 10 1068 1599
Fragments arranged by size:
1599 1068 797 108 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 418 450 845 877 959 991
Size: 119 32 267 32 395 32 82 32
Cuts at: 991 1027 1059 2273 2305 3582
Size: 36 32 1214 32 1277
Fragments arranged by size:
1277 1214 395 267 119 82 36 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1194 1688 3582
Size: 1194 494 1894
Fragments arranged by size:
1894 1194 494
KasI G'GCGC_C
Cuts at: 0 911 3582
Size: 911 2671
MfeI C'AATT_G
Cuts at: 0 666 3582
Size: 666 2916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1470 2630 3582
Size: 236 1234 1160 952
Fragments arranged by size:
1234 1160 952 236
MscI TGG'CCA
Cuts at: 0 115 1301 3582
Size: 115 1186 2281
Fragments arranged by size:
2281 1186 115
MslI CAynn'nnrTG
Cuts at: 0 508 883 1744 1861 2929 3582
Size: 508 375 861 117 1068 653
Fragments arranged by size:
1068 861 653 508 375 117
MspA1I CmG'CkG
Cuts at: 0 3237 3243 3280 3582
Size: 3237 6 37 302
Fragments arranged by size:
3237 302 37 6
NarI GG'CG_CC
Cuts at: 0 912 3582
Size: 912 2670
NcoI C'CATG_G
Cuts at: 0 1256 2924 3582
Size: 1256 1668 658
Fragments arranged by size:
1668 1256 658
NdeI CA'TA_TG
Cuts at: 0 2723 3582
Size: 2723 859
NheI G'CTAG_C
Cuts at: 0 2394 3582
Size: 2394 1188
NsiI A_TGCA'T
Cuts at: 0 2576 3582
Size: 2576 1006
NspI r_CATG'y
Cuts at: 0 1281 2490 2574 2578 3582
Size: 1281 1209 84 4 1004
Fragments arranged by size:
1281 1209 1004 84 4
PasI CC'CwG_GG
Cuts at: 0 300 3582
Size: 300 3282
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 255 3108 3582
Size: 234 21 2853 474
Fragments arranged by size:
2853 474 234 21
PfoI T'CCnGG_A
Cuts at: 0 314 1140 1214 1425 3134 3317 3582
Size: 314 826 74 211 1709 183 265
Fragments arranged by size:
1709 826 314 265 211 183 74
PmlI CAC'GTG
Cuts at: 0 1305 2748 3582
Size: 1305 1443 834
Fragments arranged by size:
1443 1305 834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 3008 3040 3582
Size: 229 32 2747 32 542
Fragments arranged by size:
2747 542 229 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3387 3582
Size: 3387 195
PshAI GACnn'nnGTC
Cuts at: 0 676 3582
Size: 676 2906
PstI C_TGCA'G
Cuts at: 0 687 1028 1274 1454 3227 3242 3395 3582
Size: 687 341 246 180 1773 15 153 187
Fragments arranged by size:
1773 687 341 246 187 180 153 15
PvuII CAG'CTG
Cuts at: 0 3243 3582
Size: 3243 339
SacI G_AGCT'C
Cuts at: 0 1889 3582
Size: 1889 1693
SacII CC_GC'GG
Cuts at: 0 3281 3582
Size: 3281 301
SapI GCTCTTCn'nnn_
Cuts at: 0 535 796 3576 3582
Size: 535 261 2780 6
Fragments arranged by size:
2780 535 261 6
SbfI CC_TGCA'GG
Cuts at: 0 3395 3582
Size: 3395 187
SexAI A'CCwGG_T
Cuts at: 0 2691 3582
Size: 2691 891
SfcI C'TryA_G
Cuts at: 0 497 683 783 1024 1270 1450 3223 3238
Size: 497 186 100 241 246 180 1773 15
Cuts at: 3238 3391 3582
Size: 153 191
Fragments arranged by size:
1773 497 246 241 191 186 180 153
100 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1307 3582
Size: 1307 2275
SfoI GGC'GCC
Cuts at: 0 913 3582
Size: 913 2669
SmaI CCC'GGG
Cuts at: 0 3174 3582
Size: 3174 408
SmlI C'TyrA_G
Cuts at: 0 1756 3582
Size: 1756 1826
SphI G_CATG'C
Cuts at: 0 2574 3582
Size: 2574 1008
StuI AGG'CCT
Cuts at: 0 755 3582
Size: 755 2827
StyI C'CwwG_G
Cuts at: 0 845 915 1256 1917 2711 2811 2924 3029
Size: 845 70 341 661 794 100 113 105
Cuts at: 3029 3582
Size: 553
Fragments arranged by size:
845 794 661 553 341 113 105 100
70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 469 827 2242 3582
Size: 469 358 1415 1340
Fragments arranged by size:
1415 1340 469 358
TatI w'GTAC_w
Cuts at: 0 590 1454 1842 2372 2504 3582
Size: 590 864 388 530 132 1078
Fragments arranged by size:
1078 864 590 530 388 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1077 1109 1382 1414 2690 2722 3582
Size: 1077 32 273 32 1276 32 860
Fragments arranged by size:
1276 1077 860 273 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 277 652 3582
Size: 277 375 2930
Fragments arranged by size:
2930 375 277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 852 1254 3582
Size: 297 555 402 2328
Fragments arranged by size:
2328 555 402 297
XmaI C'CCGG_G
Cuts at: 0 3172 3582
Size: 3172 410
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AvaI BaeI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HindIII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PstI PvuII SacI SacII SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AflII ApaI ApoI AscI AseI AsiSI
AvrII BamHI BbvCI BmgBI BmrI BsiWI BspEI BssHII
BstBI BstZ17I ClaI CspCI EcoRI FseI FspAI HincII
HpaI KpnI MluI NaeI NgoMIV NotI NruI PacI
PciI PmeI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SanDI ScaI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmnI ZraI