################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AvaI AvrII BalI BamHI BbrPI BcgI BciVI BfrI BglII BlnI BsaAI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoO65I EcoRV EcoT22I FseI HgaI HincII HindII HpaI Ksp632I MfeI MluI MluNI MroI MscI MunI NaeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 107 GGTACC G^GTACC AcyI 123 GGCGCC G[GA]^CG[CT]C AflIII 533 ACATGT A^C[GA][CT]GT AlwI 1483 GATCC GGATC(4/5) AocI 24 CCTGAGG CC^T[ACGT]AGG Asp700I 892 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 107 GGTACC G^GTACC AspI 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1297 GGACC G^G[AT]CC AviII 1394 TGCGCA TGC^GCA BbeI 123 GGCGCC GGCGC^C BbsI 1456 GAAGAC GAAGAC(2/6) BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BclI 1590 TGATCA T^GATCA BlpI 1734 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1734 GCTCAGC GC^T[ACGT]AGC BpuAI 1456 GAAGAC GAAGAC(2/6) BsaBI 811 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 123 GGCGCC G[GA]^CG[CT]C BsgI 1070 GTGCAG GTGCAG(16/14) BsiEI 205 CGGCCG CG[GA][CT]^CG BsmFI 164 GGGAC GGGAC(10/14) BspHI 1452 TCATGA T^CATGA BspLU11I 533 ACATGT A^CATGT BsrFI 144 ACCGGC [GA]^CCGG[CT] BstXI 879 CCATAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1482 AGATCC [GA]^GATC[CT] Bsu36I 24 CCTGAGG CC^T[ACGT]AGG CelII 1734 GCTCAGC GC^T[ACGT]AGC Cfr10I 144 ACCGGC [GA]^CCGG[CT] DraII 448 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1412 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 205 CGGCCG C^GGCCG EclXI 205 CGGCCG C^GGCCG Eco52I 205 CGGCCG C^GGCCG Eco81I 24 CCTGAGG CC^T[ACGT]AGG EcoNI 901 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 448 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1540 GAATTC G^AATTC FbaI 1590 TGATCA T^GATCA FspI 1394 TGCGCA TGC^GCA HaeII 123 GGCGCC [GA]GCGC^[CT] Hin1I 123 GGCGCC G[GA]^CG[CT]C HindIII 839 AAGCTT A^AGCTT KasI 123 GGCGCC G^GCGCC KpnI 107 GGTACC GGTAC^C MamI 811 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1482 AGATCC [GA]^GATC[CT] NarI 123 GGCGCC GG^CGCC NcoI 281 CCATGG C^CATGG NruI 50 TCGCGA TCG^CGA PflFI 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1232 CTGCAG CTGCA^G RcaI 1452 TCATGA T^CATGA SexAI 1413 ACCTGGT A^CC[AT]GGT SfcI 1232 CTGCAG C^T[GA][CT]AG SfoI 123 GGCGCC GGC^GCC SmlI 915 CTTGAG C^T[CT][GA]AG Tth111I 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1482 AGATCC [GA]^GATC[CT] XmnI 892 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 108 GTAC GT^AC 288 GTAC AlwNI 635 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1547 CAGATTCTG BanII 252 GAGCCC G[GA]GC[CT]^C 277 GAGCCC BfaI 1046 CTAG C^TAG 1616 CTAG BglI 115 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 212 GCCGCGGCGGC BmyI 252 GAGCCC G[AGT]GC[ACT]^C 277 GAGCCC BsmAI 911 GAGAC GTCTC(1/5) 1228 GAGAC Bsp1286I 252 GAGCCC G[AGT]GC[ACT]^C 277 GAGCCC BstNI 901 CCTGG CC^[AT]GG 1414 CCTGG EaeI 205 CGGCCG [CT]^GGCC[GA] 269 TGGCCG EcoRII 901 CCTGG ^CC[AT]GG 1414 CCTGG EcoT14I 281 CCATGG C^C[AT][AT]GG 1682 CCAAGG FauI 159 CCCGC CCCGC(4/6) 162 GCGGG KspI 160 CCGCGG CCGC^GG 213 CCGCGG MaeI 1046 CTAG C^TAG 1616 CTAG MaeIII 737 GTTAC ^GT[ACGT]AC 1401 GTTAC MvaI 901 CCTGG CC^[AT]GG 1414 CCTGG NdeI 992 CATATG CA^TATG 1223 CATATG NspI 533 ACATGT [GA]CATG^[CT] 1397 GCATGT RsaI 108 GTAC GT^AC 288 GTAC SacII 160 CCGCGG CCGC^GG 213 CCGCGG StyI 281 CCATGG C^C[AT][AT]GG 1682 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcsI AluI ApoI BanI BbvI BcnI BpmI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MseI MslI MspA1I MspI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I