Restriction map for
pFN21AB6468
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HgaI
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 217 GTCTAC GT^[AC][GT]AC
AfaI 406 GTAC GT^AC
AlwI 425 GATCC GGATC(4/5)
BalI 606 TGGCCA TGG^CCA
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
BcnI 311 CCCGG CC^[GC]GG
BfaI 367 CTAG C^TAG
BsaBI 156 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 57 CGACCG CG[GA][CT]^CG
BsmFI 264 GGGAC GGGAC(10/14)
BsmI 725 GAATGC GAATGC(1/-1)
BsrDI 243 CATTGC GCAATG(2/0)
BstAPI 301 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 17 GCGC GCG^C
Cfr13I 675 GGCCC G^G[ACGT]CC
DsaI 260 CCATGG C^C[GA][CT]GG
EcoNI 816 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 727 ATGCAT ATGCA^T
HaeII 16 AGCGCC [GA]GCGC^[CT]
HapII 312 CCGG C^CGG
HhaI 17 GCGC GCG^C
HinP1I 17 GCGC G^CGC
HincII 846 GTTAAC GT[CT]^[GA]AC
HindII 846 GTTAAC GT[CT]^[GA]AC
HindIII 410 AAGCTT A^AGCTT
HpaI 846 GTTAAC GTT^AAC
HpaII 312 CCGG C^CGG
MaeI 367 CTAG C^TAG
MaeII 10 ACGT A^CGT
MamI 156 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 606 TGGCCA TGG^CCA
MscI 606 TGGCCA TGG^CCA
MspA1I 896 CAGCTG C[AC]G^C[GT]G
MspI 312 CCGG C^CGG
NciI 311 CCCGG CC^[GC]GG
NcoI 260 CCATGG C^CATGG
NdeI 884 CATATG CA^TATG
NlaIV 874 GGTTCC GG[ACGT]^[ACGT]CC
NsiI 727 ATGCAT ATGCA^T
PvuII 896 CAGCTG CAG^CTG
RsaI 406 GTAC GT^AC
Sau96I 675 GGCCC G^G[ACGT]CC
SfcI 219 CTACAG C^T[GA][CT]AG
TaqI 56 TCGA T^CGA
Tsp45I 652 GTGAC ^GT[GC]AC
TthHB8I 56 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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BbsI 22 GAAGAC GAAGAC(2/6)
537 GAAGAC
BcgI 986 CGAAGAATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
47 GCAAGATGATCG
BglII 924 AGATCT A^GATCT
953 AGATCT
BpmI 359 CTCCAG CTGGAG(16/14)
122 CTGGAG
BpuAI 22 GAAGAC GAAGAC(2/6)
537 GAAGAC
BseRI 712 GAGGAG GAGGAG(10/8)
837 GAGGAG
BsgI 6 CTGCAC GTGCAG(16/14)
994 GTGCAG
BspHI 168 TCATGA T^CATGA
353 TCATGA
BsrI 489 CCAGT ACTGG(1/-1)
915 CCAGT
BstF5I 174 GGATG GGATG(2/0)
189 GGATG
BstNI 296 CCAGG CC^[AT]GG
428 CCAGG
Cac8I 331 GCTGGC GC[ACGT]^[ACGT]GC
412 GCTTGC
EaeI 89 CGGCCA [CT]^GGCC[GA]
606 TGGCCA
EarI 796 CTCTTC CTCTTC(1/4)
889 GAAGAG
EcoRII 296 CCAGG ^CC[AT]GG
428 CCAGG
EcoT14I 260 CCATGG C^C[AT][AT]GG
947 CCAAGG
FokI 174 GGATG GGATG(9/13)
189 GGATG
HphI 566 TCACC GGTGA(8/7)
968 TCACC
Ksp632I 796 CTCTTC CTCTTC(1/4)
889 GAAGAG
MaeIII 652 GTGAC ^GT[ACGT]AC
1015 GTTAC
MslI 141 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
169 CATGAGGATG
MvaI 296 CCAGG CC^[AT]GG
428 CCAGG
RcaI 168 TCATGA T^CATGA
353 TCATGA
SfaNI 307 GATGC GCATC(5/9)
1009 GATGC
StyI 260 CCATGG C^C[AT][AT]GG
947 CCAAGG
Tsp509I 317 AATT ^AATT
660 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstYI DdeI DpnII
Fnu4HI HaeIII HinfI ItaI MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII PleI Sau3AI ScrFI
TfiI Tru9I TseI TspRI XhoII