Restriction map for
pFN21AB6472
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BanI BanII BbeI BbsI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MseI MunI NarI
NdeI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 217 GTCTAC GT^[AC][GT]AC
AccIII 383 TCCGGA T^CCGGA
AflIII 785 ACACGT A^C[GA][CT]GT
Aor51HI 41 AGCGCT AGC^GCT
Asp700I 744 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1012 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 245 TGGCCA TGG^CCA
BamHI 635 GGATCC G^GATCC
BbrPI 972 CACGTG CAC^GTG
BbvCI 706 GCTGAGG CCTCAGC(-5/-2)
BcgI 450 CGACTCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 207 CCGGG CC^[GC]GG
BfaI 703 CTAG C^TAG
BglII 956 AGATCT A^GATCT
BsaAI 972 CACGTG [CT]AC^GT[GA]
BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 383 TCCGGA [AT]^CCGG[AT]
BseAI 383 TCCGGA T^CCGGA
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmAI 559 GAGAC GTCTC(1/5)
BsmFI 845 GTCCC GGGAC(10/14)
BsmI 193 GAATGC GAATGC(1/-1)
BspEI 383 TCCGGA T^CCGGA
BsrFI 197 GCCGGC [GA]^CCGG[CT]
BsrI 234 CCAGT ACTGG(1/-1)
BssSI 169 CACGAG CACGAG(-5/-1)
BstXI 168 CCACGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 197 GCCGGC [GA]^CCGG[CT]
DraIII 491 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco47III 41 AGCGCT AGC^GCT
Eco52I 35 CGGCCG C^GGCCG
EcoT22I 730 ATGCAT ATGCA^T
MaeI 703 CTAG C^TAG
MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 245 TGGCCA TGG^CCA
MroI 383 TCCGGA T^CCGGA
MscI 245 TGGCCA TGG^CCA
MslI 169 CACGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 197 GCCGGC GCC^GGC
NciI 207 CCGGG CC^[GC]GG
NgoMIV 197 GCCGGC G^CCGGC
NheI 702 GCTAGC G^CTAGC
NsiI 730 ATGCAT ATGCA^T
NspI 728 GCATGC [GA]CATG^[CT]
PflFI 1012 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 972 CACGTG CAC^GTG
PmlI 972 CACGTG CAC^GTG
PstI 271 CTGCAG CTGCA^G
PvuII 455 CAGCTG CAG^CTG
SapI 135 GAAGAGC GCTCTTC(1/4)
SfaNI 758 GCATC GCATC(5/9)
SphI 728 GCATGC GCATG^C
StuI 413 AGGCCT AGG^CCT
Tth111I 1012 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 545 CCACGGGGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 744 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 92 CGCG CG^CG
535 CGCG
AfaI 204 GTAC GT^AC
870 GTAC
AspHI 138 GAGCAC G[AT]GC[AT]^C
355 GTGCTC
BmyI 138 GAGCAC G[AGT]GC[ACT]^C
355 GTGCTC
BpmI 294 CTCCAG CTGGAG(16/14)
478 CTGGAG
BsiHKAI 138 GAGCAC G[AT]GC[AT]^C
355 GTGCTC
Bsp1286I 138 GAGCAC G[AGT]GC[ACT]^C
355 GTGCTC
BspMI 65 ACCTGC ACCTGC(4/8)
269 ACCTGC
BstAPI 348 GCAAGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
516 GCAAGATGTGC
BstNI 311 CCAGG CC^[AT]GG
477 CCTGG
BstUI 92 CGCG CG^CG
535 CGCG
BstYI 635 GGATCC [GA]^GATC[CT]
956 AGATCT
EcoNI 66 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
819 CCTGTTTGAGG
EcoRII 311 CCAGG ^CC[AT]GG
477 CCTGG
HinfI 127 GAGTC G^A[ACGT]TC
451 GACTC
MflI 635 GGATCC [GA]^GATC[CT]
956 AGATCT
MspA1I 455 CAGCTG C[AC]G^C[GT]G
613 CAGCGG
MvaI 311 CCAGG CC^[AT]GG
477 CCTGG
MvnI 92 CGCG CG^CG
535 CGCG
NcoI 260 CCATGG C^CATGG
402 CCATGG
PleI 451 GACTC GAGTC(4/5)
127 GAGTC
RsaI 204 GTAC GT^AC
870 GTAC
SfcI 219 CTACAG C^T[GA][CT]AG
271 CTGCAG
SmlI 429 CTCAAG C^T[CT][GA]AG
681 CTTGAG
TspRI 326 CACTG CA[GC]TG[ACGT][ACGT]^
640 CACTG
XhoII 635 GGATCC [GA]^GATC[CT]
956 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BbvI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsrBI BssKI BstF5I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspI MwoI NdeII NlaIII NlaIV
PpuMI Sau3AI Sau96I ScrFI StyI
TaqI TseI Tsp45I TthHB8I