(Circular) (Six-base) MAPSORT of: pFN21AB6472.seq Check: 5676 from: 1 to: 5770 pFN21AB6472 5770 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2223 3113 1937 Size: 286 890 4594 Fragments arranged by size: 4594 890 286 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3071 3833 5217 5485 1632 Size: 1439 762 1384 268 1917 Fragments arranged by size: 1917 1439 1384 762 268 AfeI AGC'GCT Cuts at: 2048 2048 Size: 5770 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2790 4669 5646 1203 Size: 1587 1879 977 1327 Fragments arranged by size: 1879 1587 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2343 2353 1184 Size: 1159 10 4601 Fragments arranged by size: 4601 1159 10 AlwNI CAG_nnn'CTG Cuts at: 3513 5085 3513 Size: 1572 4198 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BamHI G'GATC_C Cuts at: 2640 3100 3467 2640 Size: 460 367 4943 Fragments arranged by size: 4943 460 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 3089 1391 Size: 1698 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4799 BbvCI CC'TCA_GC Cuts at: 2712 2712 Size: 5770 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2444 2478 3961 3995 1903 Size: 34 507 34 1483 34 3678 Fragments arranged by size: 3678 1483 507 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BfrBI ATG'CAT Cuts at: 2737 2737 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2961 5765 2961 Size: 2804 2966 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 3831 4987 1790 Size: 2041 1156 2573 Fragments arranged by size: 2573 2041 1156 BmgBI CAC'GTC Cuts at: 1318 2793 1318 Size: 1475 4295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2711 1056 Size: 1655 4115 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2284 2504 4430 1239 Size: 1045 220 1926 2579 Fragments arranged by size: 2579 1926 1045 220 Bpu10I CC'TnA_GC Cuts at: 2712 2712 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2419 2707 3895 4760 5058 2419 Size: 288 1188 865 298 3131 Fragments arranged by size: 3131 1188 865 298 288 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2979 3561 493 Size: 711 1775 582 2702 Fragments arranged by size: 2702 1775 711 582 BsaBI GATnn'nnATC Cuts at: 2165 5764 2165 Size: 3599 2171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 2382 2658 3018 Size: 53 83 186 936 780 69 276 360 Cuts at: 3018 3577 3959 4594 275 Size: 559 382 635 1451 Fragments arranged by size: 1451 936 780 635 559 382 360 276 186 83 69 53 BsaWI w'CCGG_w Cuts at: 2388 4203 4601 4875 5022 5433 2388 Size: 1815 398 274 147 411 2725 Fragments arranged by size: 2725 1815 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 1178 Size: 30 165 30 690 30 4825 Fragments arranged by size: 4825 690 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2363 2735 2846 1186 Size: 1021 156 372 111 4110 Fragments arranged by size: 4110 1021 372 156 111 BseYI C'CCAG_C Cuts at: 2126 4973 5469 2126 Size: 2847 496 2427 Fragments arranged by size: 2847 2427 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1996 2323 2435 3023 1163 Size: 573 260 327 112 588 3910 Fragments arranged by size: 3910 588 573 327 260 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 3220 3981 5009 1540 Size: 275 186 42 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2147 2364 3831 3916 4987 2147 Size: 217 1467 85 1071 2930 Fragments arranged by size: 2930 1467 1071 217 85 BsmI GAATG_Cn' Cuts at: 2204 3281 3374 2204 Size: 1077 93 4600 Fragments arranged by size: 4600 1077 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2364 3089 3831 3916 4987 1391 Size: 399 357 217 725 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 725 399 357 217 85 BspEI T'CCGG_A Cuts at: 2388 5433 2388 Size: 3045 2725 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2283 3125 844 Size: 1235 204 842 3489 Fragments arranged by size: 3489 1235 842 204 BsrBI CCG'CTC Cuts at: 1394 1823 2306 2433 2629 3657 1394 Size: 429 483 127 196 1028 3507 Fragments arranged by size: 3507 1028 483 429 196 127 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 4414 4601 1700 Size: 110 392 2212 187 2869 Fragments arranged by size: 2869 2212 392 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 2174 3830 4842 2174 Size: 1656 1012 3102 Fragments arranged by size: 3102 1656 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2359 2527 794 Size: 1565 168 4037 Fragments arranged by size: 4037 1565 168 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2180 2180 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2640 2961 3100 3467 3852 3869 5321 5407 Size: 668 321 139 367 385 17 1452 86 Cuts at: 5407 5419 5765 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 668 385 367 346 321 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2265 2407 2550 513 Size: 552 939 261 142 143 3733 Fragments arranged by size: 3733 939 552 261 143 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 2644 3286 4080 4100 2644 Size: 642 794 20 4314 Fragments arranged by size: 4314 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DraIII CAC_nnn'GTG Cuts at: 2501 2501 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2250 2428 Size: 54 1425 50 275 18 210 210 178 Cuts at: 2428 3217 4106 5735 8 Size: 789 889 1629 43 Fragments arranged by size: 1629 1425 889 789 275 210 210 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 3217 1537 Size: 275 228 1177 4090 Fragments arranged by size: 4090 1177 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 2135 2810 3071 3700 1353 Size: 782 675 261 629 3423 Fragments arranged by size: 3423 782 675 629 261 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2284 2504 3071 3833 4430 5217 5485 Size: 393 652 220 567 762 597 787 268 Cuts at: 5485 1239 Size: 1524 Fragments arranged by size: 1524 787 762 652 597 567 393 268 220 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2828 2075 Size: 753 5017 EcoO109I rG'GnC_Cy Cuts at: 2584 2821 2932 2584 Size: 237 111 5422 Fragments arranged by size: 5422 237 111 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2025 2050 2694 4917 1678 Size: 347 25 644 2223 2531 Fragments arranged by size: 2531 2223 644 347 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2360 2392 4411 Size: 32 135 32 599 32 157 32 2019 Cuts at: 4411 4443 4485 4517 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2019 599 157 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 10 Size: 54 1425 343 420 3528 Fragments arranged by size: 3528 1425 420 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 3728 4087 4246 518 Size: 648 1012 1550 359 159 2042 Fragments arranged by size: 2042 1550 1012 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2462 2620 3186 3863 5011 5256 1606 Size: 856 158 566 677 1148 245 2120 Fragments arranged by size: 2120 1148 856 677 566 245 158 NaeI GCC'GGC Cuts at: 2204 2204 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 2004 2265 2407 513 Size: 552 939 261 142 3876 Fragments arranged by size: 3876 939 552 261 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2202 2202 Size: 5770 NheI G'CTAG_C Cuts at: 1052 2707 1052 Size: 1655 4115 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NsiI A_TGCA'T Cuts at: 2739 2739 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2737 3128 4673 1132 Size: 97 1508 391 1545 2229 Fragments arranged by size: 2229 1545 1508 391 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PmlI CAC'GTG Cuts at: 2979 2979 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2584 2821 2932 2584 Size: 237 111 5422 Fragments arranged by size: 5422 237 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2280 3122 830 Size: 1450 842 3478 Fragments arranged by size: 3478 1450 842 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2462 2462 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SapI GCTCTTCn'nnn_ Cuts at: 2135 2135 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 2224 2276 3118 4253 4934 5125 826 Size: 219 1179 52 842 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 842 681 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2434 2686 3874 4775 5037 820 Size: 197 926 491 252 1188 901 262 1553 Fragments arranged by size: 1553 1188 926 901 491 262 252 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 2737 3128 1132 Size: 1605 391 3774 Fragments arranged by size: 3774 1605 391 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 2420 1517 Size: 903 4867 StyI C'CwwG_G Cuts at: 513 1065 2004 2265 2407 2955 3209 513 Size: 552 939 261 142 548 254 3074 Fragments arranged by size: 3074 939 552 548 261 254 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2600 3797 3814 3967 4152 4597 Size: 1255 531 489 1197 17 153 185 445 Cuts at: 4597 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 531 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2874 4016 5728 Size: 274 80 33 51 1443 897 1142 1712 Cuts at: 5728 96 Size: 138 Fragments arranged by size: 1712 1443 1142 897 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2833 2865 5730 5762 2833 Size: 32 2865 32 2841 Fragments arranged by size: 2865 2841 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3020 1316 Size: 1704 4066 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2557 2557 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 2753 3899 2753 Size: 1146 4624 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI