Restriction map for pFN21AB6472
 (Circular) (Six-base) MAPSORT of: pFN21AB6472.seq  Check: 5676  from: 1  to: 5770



pFN21AB6472  5770 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3580     278 
   Size:         53      83     186     936    2044    2468

  Fragments arranged by size: 

                2468    2044     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2223    3113    1937 
   Size:        286     890    4594

  Fragments arranged by size: 

                4594     890     286
 
Acc65I G'GTAC_C

Cuts at:   3091    3091 
   Size:       5770
 
AclI AA'CG_TT

Cuts at:   1563    3897    4270    1563 
   Size:       2334     373    3063

  Fragments arranged by size: 

                3063    2334     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3071    3833    5217    5485    1632 
   Size:       1439     762    1384     268    1917

  Fragments arranged by size: 

                1917    1439    1384     762     268
 
AfeI AGC'GCT

Cuts at:   2048    2048 
   Size:       5770
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5573
 
AflIII A'CryG_T

Cuts at:   1203    2790    4669    5646    1203 
   Size:       1587    1879     977    1327

  Fragments arranged by size: 

                1879    1587    1327     977
 
AgeI A'CCGG_T

Cuts at:   4601    4601 
   Size:       5770
 
AhdI GACnn_n'nnGTC

Cuts at:   4499    4499 
   Size:       5770
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5770
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2343    2353    1184 
   Size:       1159      10    4601

  Fragments arranged by size: 

                4601    1159      10
 
AlwNI CAG_nnn'CTG

Cuts at:   3513    5085    3513 
   Size:       1572    4198
 
ApaLI G'TGCA_C

Cuts at:   3827    4983    3827 
   Size:       1156    4614
 
ApoI r'AATT_y

Cuts at:   1493    3079    3305    3457    5566    1493 
   Size:       1586     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1586     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4324     160 
   Size:        872    3292    1606

  Fragments arranged by size: 

                3292    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5770
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3095    1870 
   Size:         73    1152    4545

  Fragments arranged by size: 

                4545    1152      73
 
BamHI G'GATC_C

Cuts at:   2640    3100    3467    2640 
   Size:        460     367    4943

  Fragments arranged by size: 

                4943     460     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3091    4546     618 
   Size:        325     217     627    1304    1455    1842

  Fragments arranged by size: 

                1842    1455    1304     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3089    1391 
   Size:       1698    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4799
 
BbvCI CC'TCA_GC

Cuts at:   2712    2712 
   Size:       5770
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2444    2478    3961    3995    1903 
   Size:         34     507      34    1483      34    3678

  Fragments arranged by size: 

                3678    1483     507      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3662    4872    3662 
   Size:       1210    4560
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5770
 
BfrBI ATG'CAT

Cuts at:   2737    2737 
   Size:       5770
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4381     136 
   Size:        107     122      71     631     605    2709    1525

  Fragments arranged by size: 

                2709    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2961    5765    2961 
   Size:       2804    2966
 
BlpI GC'TnA_GC

Cuts at:   3187    3187 
   Size:       5770
 
Bme1580I G_kGCm'C

Cuts at:   1790    3831    4987    1790 
   Size:       2041    1156    2573

  Fragments arranged by size: 

                2573    2041    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2793    1318 
   Size:       1475    4295
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3501    4459     442 
   Size:        416      53      83     399      24    2084     958    1753

  Fragments arranged by size: 

                2084    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2711    1056 
   Size:       1655    4115
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2284    2504    4430    1239 
   Size:       1045     220    1926    2579

  Fragments arranged by size: 

                2579    1926    1045     220
 
Bpu10I CC'TnA_GC

Cuts at:   2712    2712 
   Size:       5770
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2419    2707    3895    4760    5058    2419 
   Size:        288    1188     865     298    3131

  Fragments arranged by size: 

                3131    1188     865     298     288
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4433     882 
   Size:        617    2934    2219

  Fragments arranged by size: 

                2934    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2979    3561     493 
   Size:        711    1775     582    2702

  Fragments arranged by size: 

                2702    1775     711     582
 
BsaBI GATnn'nnATC

Cuts at:   2165    5764    2165 
   Size:       3599    2171
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2313    2382    2658    3018 
   Size:         53      83     186     936     780      69     276     360

Cuts at:   3018    3577    3959    4594     275 
   Size:        559     382     635    1451

  Fragments arranged by size: 

                1451     936     780     635     559     382     360     276
                 186      83      69      53
 
BsaWI w'CCGG_w

Cuts at:   2388    4203    4601    4875    5022    5433    2388 
   Size:       1815     398     274     147     411    2725

  Fragments arranged by size: 

                2725    1815     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2093    2123    1178 
   Size:         30     165      30     690      30    4825

  Fragments arranged by size: 

                4825     690     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2207    2363    2735    2846    1186 
   Size:       1021     156     372     111    4110

  Fragments arranged by size: 

                4110    1021     372     156     111
 
BseYI C'CCAG_C

Cuts at:   2126    4973    5469    2126 
   Size:       2847     496    2427

  Fragments arranged by size: 

                2847    2427     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1996    2323    2435    3023    1163 
   Size:        573     260     327     112     588    3910

  Fragments arranged by size: 

                3910     588     573     327     260     112
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2043    3220    3981    5009    1540 
   Size:        275     186      42    1177     761    1028    2301

  Fragments arranged by size: 

                2301    1177    1028     761     275     186      42
 
BsiHKAI G_wGCw'C

Cuts at:   2147    2364    3831    3916    4987    2147 
   Size:        217    1467      85    1071    2930

  Fragments arranged by size: 

                2930    1467    1071     217      85
 
BsmI GAATG_Cn'

Cuts at:   2204    3281    3374    2204 
   Size:       1077      93    4600

  Fragments arranged by size: 

                4600    1077      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2147    2364    3089    3831    3916    4987    1391 
   Size:        399     357     217     725     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     725     399     357     217      85
            
 
BspEI T'CCGG_A

Cuts at:   2388    5433    2388 
   Size:       3045    2725
 
BspHI T'CATG_A

Cuts at:   3659    5389    3659 
   Size:       1730    4040
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2079    2283    3125     844 
   Size:       1235     204     842    3489

  Fragments arranged by size: 

                3489    1235     842     204
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2306    2433    2629    3657    1394 
   Size:        429     483     127     196    1028    3507

  Fragments arranged by size: 

                3507    1028     483     429     196     127
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4265    4439      65 
   Size:       1183    3017     174    1396

  Fragments arranged by size: 

                3017    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2202    4414    4601    1700 
   Size:        110     392    2212     187    2869

  Fragments arranged by size: 

                2869    2212     392     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5770
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5770
 
BssSI C'ACGA_G

Cuts at:   2174    3830    4842    2174 
   Size:       1656    1012    3102

  Fragments arranged by size: 

                3102    1656    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2359    2527     794 
   Size:       1565     168    4037

  Fragments arranged by size: 

                4037    1565     168
 
BstBI TT'CG_AA

Cuts at:   4574    4574 
   Size:       5770
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5770
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2180    2180 
   Size:       5770
 
BstYI r'GATC_y

Cuts at:   1972    2640    2961    3100    3467    3852    3869    5321    5407 
   Size:        668     321     139     367     385      17    1452      86

Cuts at:   5407    5419    5765    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     668     385     367     346     321     139
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2265    2407    2550     513 
   Size:        552     939     261     142     143    3733

  Fragments arranged by size: 

                3733     939     552     261     143     142
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5031

  Fragments arranged by size: 

                5031     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2644    3286    4080    4100    2644 
   Size:        642     794      20    4314

  Fragments arranged by size: 

                4314     794     642      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5770
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5735
 
DraI TTT'AAA

Cuts at:   3074    3074 
   Size:       5770
 
DraIII CAC_nnn'GTG

Cuts at:   2501    2501 
   Size:       5770
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4777     809 
   Size:        507    3461    1802

  Fragments arranged by size: 

                3461    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2040    2250    2428 
   Size:         54    1425      50     275      18     210     210     178

Cuts at:   2428    3217    4106    5735       8 
   Size:        789     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     789     275     210     210     178
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2040    3217    1537 
   Size:        275     228    1177    4090

  Fragments arranged by size: 

                4090    1177     275     228
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2135    2810    3071    3700    1353 
   Size:        782     675     261     629    3423

  Fragments arranged by size: 

                3423     782     675     629     261
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4357    4731    4877     387 
   Size:       1064     138    2768     374     146    1280

  Fragments arranged by size: 

                2768    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2284    2504    3071    3833    4430    5217    5485 
   Size:        393     652     220     567     762     597     787     268

Cuts at:   5485    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     787     762     652     597     567     393     268
                 220
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2075    2828    2075 
   Size:        753    5017
 
EcoO109I rG'GnC_Cy

Cuts at:   2584    2821    2932    2584 
   Size:        237     111    5422

  Fragments arranged by size: 

                5422     237     111
 
EcoRI G'AATT_C

Cuts at:   3079    3457    3079 
   Size:        378    5392
 
FspI TGC'GCA

Cuts at:   4276    4276 
   Size:       5770
 
HaeII r_GCGC'y

Cuts at:   1678    2025    2050    2694    4917    1678 
   Size:        347      25     644    2223    2531

  Fragments arranged by size: 

                2531    2223     644     347      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2171    2203    2360    2392    4411 
   Size:         32     135      32     599      32     157      32    2019

Cuts at:   4411    4443    4485    4517    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2019     599     157     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3114    3360     669 
   Size:        988     281    1176     246    3079

  Fragments arranged by size: 

                3079    1176     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5770
 
HpaI GTT'AAC

Cuts at:   3360    3360 
   Size:       5770
 
KpnI G_GTAC'C

Cuts at:   3095    3095 
   Size:       5770
 
MfeI C'AATT_G

Cuts at:   3369    3369 
   Size:       5770
 
MluI A'CGCG_T

Cuts at:   5646    5646 
   Size:       5770
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4884    5068    1305 
   Size:       3579     184    2007

  Fragments arranged by size: 

                3579    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2252      10 
   Size:         54    1425     343     420    3528

  Fragments arranged by size: 

                3528    1425     420     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2178    3728    4087    4246     518 
   Size:        648    1012    1550     359     159    2042

  Fragments arranged by size: 

                2042    1550    1012     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2462    2620    3186    3863    5011    5256    1606 
   Size:        856     158     566     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     856     677     566     245     158
 
NaeI GCC'GGC

Cuts at:   2204    2204 
   Size:       5770
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2265    2407     513 
   Size:        552     939     261     142    3876

  Fragments arranged by size: 

                3876     939     552     261     142
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5770
 
NgoMIV G'CCGG_C

Cuts at:   2202    2202 
   Size:       5770
 
NheI G'CTAG_C

Cuts at:   1052    2707    1052 
   Size:       1655    4115
 
NotI GC'GGCC_GC

Cuts at:   3217    3217 
   Size:       5770
 
NsiI A_TGCA'T

Cuts at:   2739    2739 
   Size:       5770
 
NspI r_CATG'y

Cuts at:   1132    1229    2737    3128    4673    1132 
   Size:         97    1508     391    1545    2229

  Fragments arranged by size: 

                2229    1545    1508     391      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5770
 
PciI A'CATG_T

Cuts at:   4669    4669 
   Size:       5770
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5770
 
PmeI GTTT'AAAC

Cuts at:   3074    3074 
   Size:       5770
 
PmlI CAC'GTG

Cuts at:   2979    2979 
   Size:       5770
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3839    3871    5352    5384    3839 
   Size:         32    1481      32    4225

  Fragments arranged by size: 

                4225    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2584    2821    2932    2584 
   Size:        237     111    5422

  Fragments arranged by size: 

                5422     237     111
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5770
 
PsiI TTA'TAA

Cuts at:   3340    3340 
   Size:       5770
 
PstI C_TGCA'G

Cuts at:    830    2280    3122     830 
   Size:       1450     842    3478

  Fragments arranged by size: 

                3478    1450     842
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5770
 
PvuII CAG'CTG

Cuts at:   2462    2462 
   Size:       5770
 
SalI G'TCGA_C

Cuts at:   1936    3112    1936 
   Size:       1176    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2135    2135 
   Size:       5770
 
SbfI CC_TGCA'GG

Cuts at:   3122    3122 
   Size:       5770
 
ScaI AGT'ACT

Cuts at:   4018    5730    4018 
   Size:       1712    4058
 
SfcI C'TryA_G

Cuts at:    826    1045    2224    2276    3118    4253    4934    5125     826 
   Size:        219    1179      52     842    1135     681     191    1471

  Fragments arranged by size: 

                1471    1179    1135     842     681     219     191      52
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5770
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5770
 
SmaI CCC'GGG

Cuts at:   1872    3097    1872 
   Size:       1225    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2434    2686    3874    4775    5037     820 
   Size:        197     926     491     252    1188     901     262    1553

  Fragments arranged by size: 

                1553    1188     926     901     491     262     252     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3561     493 
   Size:       3068    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5770
 
SphI G_CATG'C

Cuts at:   1132    2737    3128    1132 
   Size:       1605     391    3774

  Fragments arranged by size: 

                3774    1605     391
 
SspI AAT'ATT

Cuts at:      5      52    3694       5 
   Size:         47    3642    2081

  Fragments arranged by size: 

                3642    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2420    1517 
   Size:        903    4867
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2265    2407    2955    3209     513 
   Size:        552     939     261     142     548     254    3074

  Fragments arranged by size: 

                3074     939     552     548     261     254     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2111    2600    3797    3814    3967    4152    4597 
   Size:       1255     531     489    1197      17     153     185     445

Cuts at:   4597     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1197     531     489     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2874    4016    5728 
   Size:        274      80      33      51    1443     897    1142    1712

Cuts at:   5728      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1142     897     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2833    2865    5730    5762    2833 
   Size:         32    2865      32    2841

  Fragments arranged by size: 

                2865    2841      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    3020    1316 
   Size:       1704    4066
 
XbaI T'CTAG_A

Cuts at:   3106    3106 
   Size:       5770
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2557    2557 
   Size:       5770
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5770
 
XmaI C'CCGG_G

Cuts at:   1870    3095    1870 
   Size:       1225    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2753    3899    2753 
   Size:       1146    4624
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3578     276 
   Size:         53      83     186     936    2044    2468

  Fragments arranged by size: 

                2468    2044     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI     BplI
    BsiWI    BsmBI  BstZ17I   Bsu36I  EcoICRI    EcoRV     FalI     FseI
    FspAI     KasI     NarI     NruI     PacI     PfoI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SfoI
     SrfI     SwaI
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