Restriction map for
pFN21AB6507
(Linear) (Six-base) MAPSORT of: KIBB6507.seq Check: 1293 from: 1 to: 1077
KIBB6507.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 236 1077
Size: 236 841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1077
Size: 949 128
AflIII A'CryG_T
Cuts at: 0 985 1077
Size: 985 92
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 655 687 1077
Size: 655 32 390
Fragments arranged by size:
655 390 32
AvaI C'yCGr_G
Cuts at: 0 414 877 1077
Size: 414 463 200
Fragments arranged by size:
463 414 200
BamHI G'GATC_C
Cuts at: 0 647 1077
Size: 647 430
BanI G'GyrC_C
Cuts at: 0 190 1077
Size: 190 887
BanII G_rGCy'C
Cuts at: 0 119 1077
Size: 119 958
BbeI G_GCGC'C
Cuts at: 0 194 1077
Size: 194 883
BbvCI CC'TCA_GC
Cuts at: 0 694 1077
Size: 694 383
BglI GCCn_nnn'nGGC
Cuts at: 0 262 910 1077
Size: 262 648 167
Fragments arranged by size:
648 262 167
Bme1580I G_kGCm'C
Cuts at: 0 554 1077
Size: 554 523
BmgBI CAC'GTC
Cuts at: 0 351 534 1077
Size: 351 183 543
Fragments arranged by size:
543 351 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 607 856 1077
Size: 28 579 249 221
Fragments arranged by size:
579 249 221 28
Bpu10I CC'TnA_GC
Cuts at: 0 31 694 1077
Size: 31 663 383
Fragments arranged by size:
663 383 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 274 1042 1077
Size: 103 171 768 35
Fragments arranged by size:
768 171 103 35
BsaAI yAC'GTr
Cuts at: 0 986 1077
Size: 986 91
BsaBI GATnn'nnATC
Cuts at: 0 178 1077
Size: 178 899
BsaHI Gr'CG_yC
Cuts at: 0 104 191 902 1077
Size: 104 87 711 175
Fragments arranged by size:
711 175 104 87
BsaWI w'CCGG_w
Cuts at: 0 602 737 764 1077
Size: 602 135 27 313
Fragments arranged by size:
602 313 135 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 1077
Size: 442 30 605
Fragments arranged by size:
605 442 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 760 789 1077
Size: 220 540 29 288
Fragments arranged by size:
540 288 220 29
BseYI C'CCAG_C
Cuts at: 0 524 893 1077
Size: 524 369 184
Fragments arranged by size:
524 369 184
BsiEI CG_ry'CG
Cuts at: 0 438 631 1028 1077
Size: 438 193 397 49
Fragments arranged by size:
438 397 193 49
BsiHKAI G_wGCw'C
Cuts at: 0 119 160 1077
Size: 119 41 917
Fragments arranged by size:
917 119 41
BsmI GAATG_Cn'
Cuts at: 0 433 1077
Size: 433 644
BsmBI CGTCTCn'nnnn_
Cuts at: 0 277 1077
Size: 277 800
Bsp1286I G_dGCh'C
Cuts at: 0 119 160 554 1077
Size: 119 41 394 523
Fragments arranged by size:
523 394 119 41
BspEI T'CCGG_A
Cuts at: 0 602 764 1077
Size: 602 162 313
Fragments arranged by size:
602 313 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 524 839 1077
Size: 524 315 238
Fragments arranged by size:
524 315 238
BsrBI CCG'CTC
Cuts at: 0 149 636 1077
Size: 149 487 441
Fragments arranged by size:
487 441 149
BsrFI r'CCGG_y
Cuts at: 0 193 559 1077
Size: 193 366 518
Fragments arranged by size:
518 366 193
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1077
Size: 534 543
BstEII G'GTnAC_C
Cuts at: 0 360 1077
Size: 360 717
BstYI r'GATC_y
Cuts at: 0 293 647 845 1077
Size: 293 354 198 232
Fragments arranged by size:
354 293 232 198
BtgI C'CryG_G
Cuts at: 0 186 278 1077
Size: 186 92 799
Fragments arranged by size:
799 186 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 50 483 1077
Size: 50 433 594
Fragments arranged by size:
594 433 50
BtsI GCAGTG_nn'
Cuts at: 0 651 1077
Size: 651 426
DraIII CAC_nnn'GTG
Cuts at: 0 864 1077
Size: 864 213
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1077
Size: 9 1068
EaeI y'GGCC_r
Cuts at: 0 1025 1077
Size: 1025 52
EagI C'GGCC_G
Cuts at: 0 1025 1077
Size: 1025 52
EarI CTCTTCn'nnn_
Cuts at: 0 148 817 1077
Size: 148 669 260
Fragments arranged by size:
669 260 148
EcoICRI GAG'CTC
Cuts at: 0 117 1077
Size: 117 960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 607 856 949 1077
Size: 28 579 249 93 128
Fragments arranged by size:
579 249 128 93 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 835 1077
Size: 406 429 242
Fragments arranged by size:
429 406 242
EcoO109I rG'GnC_Cy
Cuts at: 0 411 828 1077
Size: 411 417 249
Fragments arranged by size:
417 411 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 1077
Size: 237 840
FspI TGC'GCA
Cuts at: 0 178 1077
Size: 178 899
FspAI rTGC'GCAy
Cuts at: 0 178 1077
Size: 178 899
HaeII r_GCGC'y
Cuts at: 0 194 1077
Size: 194 883
HincII GTy'rAC
Cuts at: 0 492 1077
Size: 492 585
KasI G'GCGC_C
Cuts at: 0 190 1077
Size: 190 887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 611 704 1077
Size: 485 126 93 373
Fragments arranged by size:
485 373 126 93
MslI CAynn'nnrTG
Cuts at: 0 256 1077
Size: 256 821
MspA1I CmG'CkG
Cuts at: 0 84 627 897 1077
Size: 84 543 270 180
Fragments arranged by size:
543 270 180 84
NaeI GCC'GGC
Cuts at: 0 195 1077
Size: 195 882
NarI GG'CG_CC
Cuts at: 0 191 1077
Size: 191 886
NcoI C'CATG_G
Cuts at: 0 278 1077
Size: 278 799
NgoMIV G'CCGG_C
Cuts at: 0 193 1077
Size: 193 884
PflMI CCAn_nnn'nTGG
Cuts at: 0 714 1077
Size: 714 363
PfoI T'CCnGG_A
Cuts at: 0 338 422 1077
Size: 338 84 655
Fragments arranged by size:
655 338 84
PmlI CAC'GTG
Cuts at: 0 986 1077
Size: 986 91
PpuMI rG'GwC_Cy
Cuts at: 0 411 828 1077
Size: 411 417 249
Fragments arranged by size:
417 411 249
PstI C_TGCA'G
Cuts at: 0 1049 1077
Size: 1049 28
PvuII CAG'CTG
Cuts at: 0 84 1077
Size: 84 993
SacI G_AGCT'C
Cuts at: 0 119 1077
Size: 119 958
SapI GCTCTTCn'nnn_
Cuts at: 0 148 1077
Size: 148 929
ScaI AGT'ACT
Cuts at: 0 475 1077
Size: 475 602
SexAI A'CCwGG_T
Cuts at: 0 860 1070 1077
Size: 860 210 7
Fragments arranged by size:
860 210 7
SfcI C'TryA_G
Cuts at: 0 1045 1077
Size: 1045 32
SfoI GGC'GCC
Cuts at: 0 192 1077
Size: 192 885
SmaI CCC'GGG
Cuts at: 0 416 1077
Size: 416 661
SmlI C'TyrA_G
Cuts at: 0 118 289 1057 1077
Size: 118 171 768 20
Fragments arranged by size:
768 171 118 20
StyI C'CwwG_G
Cuts at: 0 278 506 1077
Size: 278 228 571
Fragments arranged by size:
571 278 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 538 1077
Size: 499 39 539
Fragments arranged by size:
539 499 39
TatI w'GTAC_w
Cuts at: 0 473 851 1064 1077
Size: 473 378 213 13
Fragments arranged by size:
473 378 213 13
Tth111I GACn'n_nGTC
Cuts at: 0 709 1077
Size: 709 368
XmaI C'CCGG_G
Cuts at: 0 414 1077
Size: 414 663
XmnI GAAnn'nnTTC
Cuts at: 0 247 934 1077
Size: 247 687 143
Fragments arranged by size:
687 247 143
Enzymes that do cut:
AccI AcuI AflIII AloI AvaI BamHI BanI BanII
BbeI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII HincII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV PflMI PfoI PmlI PpuMI
PstI PvuII SacI SapI ScaI SexAI SfcI SfoI
SmaI SmlI StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BsaI BsgI BsiWI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I ClaI CspCI DraI EciI EcoRI EcoRV FseI
Hin4I HindIII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI ZraI