(Circular) (Six-base) MAPSORT of: pFN21AE0345.seq Check: 1688 from: 1 to: 6412 pFN21AE0345 6412 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3328 3328 Size: 6412 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4222 278 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3755 1937 Size: 1818 4594 Acc65I G'GTAC_C Cuts at: 3307 3733 3307 Size: 426 5986 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3259 4475 5859 6127 1632 Size: 1627 1216 1384 268 1917 Fragments arranged by size: 1917 1627 1384 1216 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 5311 6288 1203 Size: 4108 977 1327 Fragments arranged by size: 4108 1327 977 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 3545 1166 Size: 2379 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AlwNI CAG_nnn'CTG Cuts at: 3529 3575 4155 5727 3529 Size: 46 580 1572 4214 Fragments arranged by size: 4214 1572 580 46 ApaI G_GGCC'C Cuts at: 2856 3073 2856 Size: 217 6195 ApaLI G'TGCA_C Cuts at: 4469 5625 4469 Size: 1156 5256 ApoI r'AATT_y Cuts at: 1493 3721 3947 4099 6208 1493 Size: 2228 226 152 2109 1697 Fragments arranged by size: 2228 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4966 160 Size: 872 3934 1606 Fragments arranged by size: 3934 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2901 2921 3116 3737 1870 Size: 73 958 20 195 621 4545 Fragments arranged by size: 4545 958 621 195 73 20 BamHI G'GATC_C Cuts at: 3246 3742 4109 3246 Size: 496 367 5549 Fragments arranged by size: 5549 496 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 2675 3307 3594 3733 Size: 325 217 627 617 271 632 287 139 Cuts at: 3733 5188 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 632 627 617 325 287 271 217 139 BanII G_rGCy'C Cuts at: 1391 2235 2467 2856 2921 3073 3510 3731 1391 Size: 844 232 389 65 152 437 221 4072 Fragments arranged by size: 4072 844 437 389 232 221 152 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5441 BbvCI CC'TCA_GC Cuts at: 2086 2307 2754 2086 Size: 221 447 5744 Fragments arranged by size: 5744 447 221 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2239 2273 3657 3691 4603 4637 1903 Size: 34 302 34 1384 34 912 34 3678 Fragments arranged by size: 3678 1384 912 302 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4304 5514 4304 Size: 1210 5202 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3390 5023 136 Size: 107 122 71 631 605 1718 1633 1525 Fragments arranged by size: 1718 1633 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3075 6407 3075 Size: 3332 3080 BlpI GC'TnA_GC Cuts at: 2778 2846 3829 2778 Size: 68 983 5361 Fragments arranged by size: 5361 983 68 Bme1580I G_kGCm'C Cuts at: 1790 2680 2856 3073 3146 3368 3428 4473 5629 Size: 890 176 217 73 222 60 1045 1156 Cuts at: 5629 1790 Size: 2573 Fragments arranged by size: 2573 1156 1045 890 222 217 176 73 60 BmgBI CAC'GTC Cuts at: 1318 2818 3487 1318 Size: 1500 669 4243 Fragments arranged by size: 4243 1500 669 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2829 4143 5101 Size: 416 53 83 399 24 1412 1314 958 Cuts at: 5101 442 Size: 1753 Fragments arranged by size: 1753 1412 1314 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3605 3667 5072 1239 Size: 2366 62 1405 2579 Fragments arranged by size: 2579 2366 1405 62 Bpu10I CC'TnA_GC Cuts at: 2086 2307 2754 2086 Size: 221 447 5744 Fragments arranged by size: 5744 447 221 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4537 5402 5700 4537 Size: 865 298 5249 Fragments arranged by size: 5249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3581 5075 882 Size: 617 2082 1494 2219 Fragments arranged by size: 2219 2082 1494 617 BsaAI yAC'GTr Cuts at: 493 1204 4203 493 Size: 711 2999 2702 Fragments arranged by size: 2999 2702 711 BsaBI GATnn'nnATC Cuts at: 6406 6406 Size: 6412 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4219 4601 5236 275 Size: 53 83 186 936 2686 382 635 1451 Fragments arranged by size: 2686 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2964 3399 4845 5243 5517 5664 6075 2964 Size: 435 1446 398 274 147 411 3301 Fragments arranged by size: 3301 1446 435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2805 2835 2882 2912 3026 Size: 30 165 30 1402 30 47 30 114 Cuts at: 3026 3056 3086 3116 3203 3233 1178 Size: 30 30 30 87 30 4357 Fragments arranged by size: 4357 1402 165 114 87 47 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3518 1186 Size: 2332 4080 BseYI C'CCAG_C Cuts at: 2026 2480 3060 3284 3419 3663 5615 6111 2026 Size: 454 580 224 135 244 1952 496 2327 Fragments arranged by size: 2327 1952 580 496 454 244 224 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3432 3500 1163 Size: 573 1696 68 4075 Fragments arranged by size: 4075 1696 573 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2736 3862 4623 5651 1540 Size: 275 186 735 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 735 275 186 BsiHKAI G_wGCw'C Cuts at: 2156 2793 4473 4558 5629 2156 Size: 637 1680 85 1071 2939 Fragments arranged by size: 2939 1680 1071 637 85 BsmI GAATG_Cn' Cuts at: 2245 3923 4016 2245 Size: 1678 93 4641 Fragments arranged by size: 4641 1678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2651 2808 2651 Size: 157 6255 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2235 2467 2680 2793 2856 2921 Size: 399 366 79 232 213 113 63 65 Cuts at: 2921 3073 3146 3368 3428 3510 3731 4473 4558 Size: 152 73 222 60 82 221 742 85 Cuts at: 4558 5629 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 232 222 221 213 152 113 85 82 79 73 65 63 60 BspEI T'CCGG_A Cuts at: 3399 6075 3399 Size: 2676 3736 BspHI T'CATG_A Cuts at: 2235 4301 6031 2235 Size: 2066 1730 2616 Fragments arranged by size: 2616 2066 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3328 3767 844 Size: 2484 439 3489 Fragments arranged by size: 3489 2484 439 BsrBI CCG'CTC Cuts at: 1394 1823 2845 3231 3352 4299 1394 Size: 429 1022 386 121 947 3507 Fragments arranged by size: 3507 1022 947 429 386 121 BsrDI GCAATG_nn' Cuts at: 65 1248 2208 4907 5081 65 Size: 1183 960 2699 174 1396 Fragments arranged by size: 2699 1396 1183 960 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3704 5056 5243 1700 Size: 110 1894 1352 187 2869 Fragments arranged by size: 2869 1894 1352 187 110 BsrGI T'GTAC_A Cuts at: 96 2115 2826 96 Size: 2019 711 3682 Fragments arranged by size: 3682 2019 711 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 4472 5484 4472 Size: 1012 5400 BstAPI GCAn_nnn'nTGC Cuts at: 794 2210 3575 794 Size: 1416 1365 3631 Fragments arranged by size: 3631 1416 1365 BstBI TT'CG_AA Cuts at: 5216 5216 Size: 6412 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6412 BstXI CCAn_nnnn'nTGG Cuts at: 2447 3010 3451 2447 Size: 563 441 5408 Fragments arranged by size: 5408 563 441 BstYI r'GATC_y Cuts at: 1972 2415 3075 3246 3742 4109 4494 4511 5963 Size: 443 660 171 496 367 385 17 1452 Cuts at: 5963 6049 6061 6407 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 660 496 443 385 367 346 171 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2130 2136 2511 3438 3498 513 Size: 552 975 90 6 375 927 60 3427 Fragments arranged by size: 3427 975 927 552 375 90 60 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3610 487 Size: 683 56 2384 3289 Fragments arranged by size: 3289 2384 683 56 BtsI GCAGTG_nn' Cuts at: 2156 2174 3144 3184 3444 3576 3928 4722 4742 Size: 18 970 40 260 132 352 794 20 Cuts at: 4742 2156 Size: 3826 Fragments arranged by size: 3826 970 794 352 260 132 40 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6377 DraI TTT'AAA Cuts at: 3716 3716 Size: 6412 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3395 5419 809 Size: 507 2079 2024 1802 Fragments arranged by size: 2079 2024 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2133 2664 Size: 54 1425 50 275 18 207 96 531 Cuts at: 2664 2733 3391 3441 3453 3859 4748 6377 8 Size: 69 658 50 12 406 889 1629 43 Fragments arranged by size: 1629 1425 889 658 531 406 275 207 96 69 54 50 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2733 3859 1537 Size: 275 921 1126 4090 Fragments arranged by size: 4090 1126 921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 2477 4342 1353 Size: 923 201 1865 3423 Fragments arranged by size: 3423 1865 923 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3178 4999 5373 5519 387 Size: 1064 138 1589 1821 374 146 1280 Fragments arranged by size: 1821 1589 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3259 3605 3667 4475 5072 5859 6127 Size: 393 1627 346 62 808 597 787 268 Cuts at: 6127 1239 Size: 1524 Fragments arranged by size: 1627 1524 808 787 597 393 346 268 62 EcoO109I rG'GnC_Cy Cuts at: 2758 2932 2985 2998 2758 Size: 174 53 13 6172 Fragments arranged by size: 6172 174 53 13 EcoRI G'AATT_C Cuts at: 3721 4099 3721 Size: 378 6034 FspI TGC'GCA Cuts at: 4918 4918 Size: 6412 HaeII r_GCGC'y Cuts at: 1678 5559 1678 Size: 3881 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 3263 3295 5053 Size: 32 135 32 602 32 1057 32 1758 Cuts at: 5053 5085 5127 5159 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1758 1057 602 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4002 669 Size: 988 281 1818 246 3079 Fragments arranged by size: 3079 1818 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KpnI G_GTAC'C Cuts at: 3311 3737 3311 Size: 426 5986 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5526 5710 1305 Size: 4221 184 2007 Fragments arranged by size: 4221 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 3455 10 Size: 54 1425 343 834 789 2967 Fragments arranged by size: 2967 1425 834 789 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3545 3629 4370 4729 4888 518 Size: 648 2379 84 741 359 159 2042 Fragments arranged by size: 2379 2042 741 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2138 2443 2491 2641 2753 2797 2850 3243 Size: 532 305 48 150 112 44 53 393 Cuts at: 3243 3671 3828 4505 5653 5898 1606 Size: 428 157 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 532 428 393 305 245 157 150 112 53 48 44 NaeI GCC'GGC Cuts at: 3706 3706 Size: 6412 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5860 NdeI CA'TA_TG Cuts at: 387 387 Size: 6412 NgoMIV G'CCGG_C Cuts at: 3704 3704 Size: 6412 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2833 3770 5315 1132 Size: 97 1604 937 1545 2229 Fragments arranged by size: 2229 1604 1545 937 97 PasI CC'CwG_GG Cuts at: 1348 2460 1348 Size: 1112 5300 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 1108 Size: 1143 5269 PfoI T'CCnGG_A Cuts at: 3683 3683 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 2985 2998 2758 Size: 227 13 6172 Fragments arranged by size: 6172 227 13 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 3982 3982 Size: 6412 PspOMI G'GGCC_C Cuts at: 2852 3069 2852 Size: 217 6195 PstI C_TGCA'G Cuts at: 830 3580 3764 830 Size: 2750 184 3478 Fragments arranged by size: 3478 2750 184 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 PvuII CAG'CTG Cuts at: 2491 2641 2753 3671 2491 Size: 150 112 918 5232 Fragments arranged by size: 5232 918 150 112 RsrII CG'GwC_CG Cuts at: 2550 2550 Size: 6412 SacII CC_GC'GG Cuts at: 2139 2139 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SanDI GG'GwC_CC Cuts at: 2998 2998 Size: 6412 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 3576 3760 4895 5576 5767 826 Size: 219 2531 184 1135 681 191 1471 Fragments arranged by size: 2531 1471 1135 681 219 191 184 SfiI GGCCn_nnn'nGGCC Cuts at: 3390 3390 Size: 6412 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6412 SmaI CCC'GGG Cuts at: 1872 2903 3739 1872 Size: 1031 836 4545 Fragments arranged by size: 4545 1031 836 SmlI C'TyrA_G Cuts at: 820 1017 1943 4516 5417 5679 820 Size: 197 926 2573 901 262 1553 Fragments arranged by size: 2573 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 3770 1132 Size: 2638 3774 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 2684 1517 Size: 1167 5245 StyI C'CwwG_G Cuts at: 513 1065 2094 3222 3851 513 Size: 552 1029 1128 629 3074 Fragments arranged by size: 3074 1128 1029 629 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3620 4439 4456 4609 4794 5239 325 Size: 1255 2040 819 17 153 185 445 1498 Fragments arranged by size: 2040 1498 1255 819 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 2826 4658 Size: 274 80 33 51 1443 138 711 1832 Cuts at: 4658 6370 96 Size: 1712 138 Fragments arranged by size: 1832 1712 1443 711 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2805 2837 3086 3118 6372 6404 2805 Size: 32 249 32 3254 32 2813 Fragments arranged by size: 3254 2813 249 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 3748 3748 Size: 6412 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2488 2128 Size: 360 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6412 XmaI C'CCGG_G Cuts at: 1870 2901 3737 1870 Size: 1031 836 4545 Fragments arranged by size: 4545 1031 836 XmnI GAAnn'nnTTC Cuts at: 4541 4541 Size: 6412 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4220 276 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacI SapI SexAI SfoI SrfI SwaI