Restriction map for
pFN21AB7694
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BlnI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 337 CGCG CG^CG
Alw44I 771 GTGCAC G^TGCAC
AocI 112 CCTGAGG CC^T[ACGT]AGG
ApaLI 771 GTGCAC G^TGCAC
AvaI 147 CCCGAG C^[CT]CG[GA]G
BbsI 408 GAAGAC GAAGAC(2/6)
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BcnI 527 CCGGG CC^[GC]GG
BglI 280 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 366 AGATCT A^GATCT
BpuAI 408 GAAGAC GAAGAC(2/6)
BseRI 347 CTCCTC GAGGAG(10/8)
BsgI 449 CTGCAC GTGCAG(16/14)
BsmAI 400 GAGAC GTCTC(1/5)
BsoBI 147 CCCGAG C^[CT]CG[GA]G
BstUI 337 CGCG CG^CG
BstXI 389 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 112 CCTGAGG CC^T[ACGT]AGG
CfoI 738 GCGC GCG^C
DraIII 865 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 877 CGGCCA [CT]^GGCC[GA]
Eco81I 112 CCTGAGG CC^T[ACGT]AGG
EcoNI 182 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1092 CCAAGG C^C[AT][AT]GG
HaeII 737 AGCGCC [GA]GCGC^[CT]
HgaI 193 GACGC GACGC(5/10)
HhaI 738 GCGC GCG^C
HinP1I 738 GCGC G^CGC
HindIII 565 AAGCTT A^AGCTT
MslI 1212 CACTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 337 CGCG CG^CG
NaeI 280 GCCGGC GCC^GGC
NciI 527 CCGGG CC^[GC]GG
NdeI 275 CATATG CA^TATG
NgoMIV 280 GCCGGC G^CCGGC
PstI 164 CTGCAG CTGCA^G
SexAI 866 ACCAGGT A^CC[AT]GGT
StyI 1092 CCAAGG C^C[AT][AT]GG
TaqI 912 TCGA T^CGA
TfiI 85 GAATC G^A[AT]TC
TthHB8I 912 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 1073 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1118 CAGGGCCTG
AspHI 150 GAGCAC G[AT]GC[AT]^C
771 GTGCAC
BlpI 420 GCTGAGC GC^T[ACGT]AGC
1230 GCTCAGC
BpmI 954 CTCCAG CTGGAG(16/14)
397 CTGGAG
Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC
1230 GCTCAGC
BsiHKAI 150 GAGCAC G[AT]GC[AT]^C
771 GTGCAC
BspMI 447 ACCTGC ACCTGC(4/8)
760 GCAGGT
BsrBI 1228 CCGCTC CCGCTC(-3/-3)
415 GAGCGG
BsrDI 236 CATTGC GCAATG(2/0)
836 GCAATG
BsrFI 280 GCCGGC [GA]^CCGG[CT]
875 ACCGGC
CelII 420 GCTGAGC GC^T[ACGT]AGC
1230 GCTCAGC
Cfr10I 280 GCCGGC [GA]^CCGG[CT]
875 ACCGGC
DsaI 359 CCGTGG C^C[GA][CT]GG
1169 CCACGG
FauI 335 CCCGC CCCGC(4/6)
1028 GCGGG
PleI 343 GACTC GAGTC(4/5)
909 GACTC
PvuII 167 CAGCTG CAG^CTG
989 CAGCTG
SfaNI 124 GATGC GCATC(5/9)
925 GCATC
Tsp509I 541 AATT ^AATT
1033 AATT
XcmI 274 CCATATGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1208 CCAGCACTACTGTGG
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AvaII BamHI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI StuI
TseI TspRI Van91I XhoII