(Circular) (Six-base) MAPSORT of: pFN21AB7694.seq Check: 6414 from: 1 to: 5944 pFN21AB7694 5944 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3039 4007 5391 5659 1632 Size: 1407 968 1384 268 1917 Fragments arranged by size: 1917 1407 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 4673 4673 Size: 5944 AleI CACnn'nnGTG Cuts at: 1166 3221 1166 Size: 2055 3889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5944 AlwNI CAG_nnn'CTG Cuts at: 3083 3128 3687 5259 3083 Size: 45 559 1572 3768 Fragments arranged by size: 3768 1572 559 45 ApaI G_GGCC'C Cuts at: 2501 3074 3110 2501 Size: 573 36 5335 Fragments arranged by size: 5335 573 36 ApaLI G'TGCA_C Cuts at: 2776 4001 5157 2776 Size: 1225 1156 3563 Fragments arranged by size: 3563 1225 1156 ApoI r'AATT_y Cuts at: 1493 3253 3479 3631 5740 1493 Size: 1760 226 152 2109 1697 Fragments arranged by size: 2109 1760 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4498 160 Size: 872 3466 1606 Fragments arranged by size: 3466 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2152 3269 1870 Size: 73 209 1117 4545 Fragments arranged by size: 4545 1117 209 73 BamHI G'GATC_C Cuts at: 2062 2262 2830 3274 3641 2062 Size: 200 568 444 367 4365 Fragments arranged by size: 4365 568 444 367 200 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3265 4720 618 Size: 325 217 627 1478 1455 1842 Fragments arranged by size: 1842 1478 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2276 2313 2432 2501 2540 2960 3074 Size: 655 230 37 119 69 39 420 114 Cuts at: 3074 3110 3170 3182 3263 1391 Size: 36 60 12 81 4072 Fragments arranged by size: 4072 655 420 230 119 114 81 69 60 39 37 36 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 928 Size: 971 521 4452 Fragments arranged by size: 4452 971 521 BbvCI CC'TCA_GC Cuts at: 2202 2202 Size: 5944 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2291 4555 136 Size: 107 122 71 631 605 619 2264 1525 Fragments arranged by size: 2264 1525 631 619 605 122 107 71 BglII A'GATC_T Cuts at: 2371 5939 2371 Size: 3568 2376 BlpI GC'TnA_GC Cuts at: 2426 3236 3361 2426 Size: 810 125 5009 Fragments arranged by size: 5009 810 125 Bme1580I G_kGCm'C Cuts at: 1790 2501 2780 3074 3110 4005 5161 1790 Size: 711 279 294 36 895 1156 2573 Fragments arranged by size: 2573 1156 895 711 294 279 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2047 2216 3077 Size: 416 53 83 399 24 630 169 861 Cuts at: 3077 3173 3675 4633 442 Size: 96 502 958 1753 Fragments arranged by size: 1753 958 861 630 502 416 399 169 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2944 4604 1239 Size: 1184 521 1660 2579 Fragments arranged by size: 2579 1660 1184 521 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 5944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4069 4934 5232 4069 Size: 865 298 4781 Fragments arranged by size: 4781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3751 4133 4768 275 Size: 53 83 186 936 2218 382 635 1451 Fragments arranged by size: 2218 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4377 4775 5049 5196 5607 4377 Size: 398 274 147 411 4714 Fragments arranged by size: 4714 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 2623 2653 1178 Size: 30 165 30 798 30 392 30 4469 Fragments arranged by size: 4469 798 392 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2343 1186 Size: 1157 4787 BseYI C'CCAG_C Cuts at: 2289 3017 5147 5643 2289 Size: 728 2130 496 2590 Fragments arranged by size: 2590 2130 728 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2439 1163 Size: 573 703 4668 Fragments arranged by size: 4668 703 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3394 4155 5183 1540 Size: 275 186 1393 761 1028 2301 Fragments arranged by size: 2301 1393 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2159 2780 4005 4090 5161 2159 Size: 621 1225 85 1071 2942 Fragments arranged by size: 2942 1225 1071 621 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2159 2276 2313 2432 2501 2540 Size: 399 256 113 117 37 119 69 39 Cuts at: 2540 2780 2960 3074 3110 3170 3182 3263 4005 Size: 240 180 114 36 60 12 81 742 Cuts at: 4005 4090 5161 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 256 240 180 119 117 114 113 85 81 69 60 39 37 36 12 BspEI T'CCGG_A Cuts at: 5607 5607 Size: 5944 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2461 2756 3299 844 Size: 1617 295 543 3489 Fragments arranged by size: 3489 1617 543 295 BsrBI CCG'CTC Cuts at: 1394 1823 2422 3235 3831 1394 Size: 429 599 813 596 3507 Fragments arranged by size: 3507 813 599 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2240 2848 4439 4613 65 Size: 1183 992 608 1591 174 1396 Fragments arranged by size: 1591 1396 1183 992 608 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2285 2880 4588 4775 1700 Size: 110 475 595 1708 187 2869 Fragments arranged by size: 2869 1708 595 475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 4004 5016 4004 Size: 1012 4932 BstAPI GCAn_nnn'nTGC Cuts at: 794 2685 2784 2847 794 Size: 1891 99 63 3891 Fragments arranged by size: 3891 1891 99 63 BstBI TT'CG_AA Cuts at: 4748 4748 Size: 5944 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5944 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2401 Size: 5944 BstYI r'GATC_y Cuts at: 1972 2062 2262 2371 2830 3274 3641 4026 4043 Size: 90 200 109 459 444 367 385 17 Cuts at: 4043 5495 5581 5593 5939 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 459 444 385 367 346 200 109 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2118 2118 Size: 5944 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 3174 513 Size: 552 939 360 810 3283 Fragments arranged by size: 3283 939 810 552 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5205 Fragments arranged by size: 5205 683 56 BtsI GCAGTG_nn' Cuts at: 2145 2456 3460 4254 4274 2145 Size: 311 1004 794 20 3815 Fragments arranged by size: 3815 1004 794 311 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2557 2592 529 Size: 35 1993 35 3881 Fragments arranged by size: 3881 1993 35 35 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DraIII CAC_nnn'GTG Cuts at: 2875 2875 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2882 3391 4280 Size: 54 1425 50 275 18 1052 509 889 Cuts at: 4280 5909 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1052 889 509 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3391 1537 Size: 275 1579 4090 Fragments arranged by size: 4090 1579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3874 1353 Size: 2521 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2665 2827 4531 4905 5051 387 Size: 1064 138 1076 162 1704 374 146 1280 Fragments arranged by size: 1704 1280 1076 1064 374 162 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2944 3039 4007 4604 5391 5659 Size: 393 791 521 95 968 597 787 268 Cuts at: 5659 1239 Size: 1524 Fragments arranged by size: 1524 968 791 787 597 521 393 268 95 EcoNI CCTnn'n_nnAGG Cuts at: 2191 2191 Size: 5944 EcoO109I rG'GnC_Cy Cuts at: 2025 2034 2450 2497 2498 2721 3071 3107 3125 Size: 9 416 47 1 223 350 36 18 Cuts at: 3125 3194 2025 Size: 69 4775 Fragments arranged by size: 4775 416 350 223 69 47 36 18 9 1 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2746 5091 1678 Size: 1068 2345 2531 Fragments arranged by size: 2531 2345 1068 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4585 4617 4659 4691 1373 Size: 32 135 32 3013 32 42 32 2626 Fragments arranged by size: 3013 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 2570 748 Size: 1822 4122 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5058 5242 1305 Size: 3753 184 2007 Fragments arranged by size: 3753 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4122 Fragments arranged by size: 4122 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3221 3902 4261 4420 518 Size: 648 2055 681 359 159 2042 Fragments arranged by size: 2055 2042 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2168 2174 2996 3027 3360 4037 5185 5430 Size: 562 6 822 31 333 677 1148 245 Cuts at: 5430 1606 Size: 2120 Fragments arranged by size: 2120 1148 822 677 562 333 245 31 6 NaeI GCC'GGC Cuts at: 2287 2287 Size: 5944 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4453 Fragments arranged by size: 4453 939 552 NdeI CA'TA_TG Cuts at: 387 2281 387 Size: 1894 4050 NgoMIV G'CCGG_C Cuts at: 2285 2285 Size: 5944 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3302 4847 1132 Size: 97 2073 1545 2229 Fragments arranged by size: 2229 2073 1545 97 PasI CC'CwG_GG Cuts at: 1348 2029 2267 2446 1348 Size: 681 238 179 4846 Fragments arranged by size: 4846 681 238 179 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2059 2365 2999 3103 1108 Size: 951 306 634 104 3949 Fragments arranged by size: 3949 951 634 306 104 PfoI T'CCnGG_A Cuts at: 2400 2400 Size: 5944 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2025 2034 2450 2025 Size: 9 416 5519 Fragments arranged by size: 5519 416 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PspOMI G'GGCC_C Cuts at: 2497 3070 3106 2497 Size: 573 36 5335 Fragments arranged by size: 5335 573 36 PstI C_TGCA'G Cuts at: 830 2173 3296 830 Size: 1343 1123 3478 Fragments arranged by size: 3478 1343 1123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2174 2996 2174 Size: 822 5122 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SanDI GG'GwC_CC Cuts at: 2025 2025 Size: 5944 SbfI CC_TGCA'GG Cuts at: 3296 3296 Size: 5944 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SexAI A'CCwGG_T Cuts at: 2871 2871 Size: 5944 SfcI C'TryA_G Cuts at: 826 1045 2169 2191 2209 3292 4427 5108 5299 Size: 219 1124 22 18 1083 1135 681 191 Cuts at: 5299 826 Size: 1471 Fragments arranged by size: 1471 1135 1124 1083 681 219 191 22 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5944 SmaI CCC'GGG Cuts at: 1872 3271 1872 Size: 1399 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4048 4949 5211 820 Size: 197 926 2105 901 262 1553 Fragments arranged by size: 2105 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 2207 3060 3102 1517 Size: 690 853 42 4359 Fragments arranged by size: 4359 853 690 42 StyI C'CwwG_G Cuts at: 513 1065 2004 3097 3383 513 Size: 552 939 1093 286 3074 Fragments arranged by size: 3074 1093 939 552 286 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3187 3971 3988 4141 4326 4771 325 Size: 1255 1607 784 17 153 185 445 1498 Fragments arranged by size: 1607 1498 1255 784 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2460 2492 2822 2854 5904 5936 2460 Size: 32 330 32 3050 32 2468 Fragments arranged by size: 3050 2468 330 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2286 3220 2286 Size: 934 5010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 3269 1870 Size: 1399 4545 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI