Restriction map for
pFN21AB7694
(Linear) (Six-base) MAPSORT of: KIBB7694.seq Check: 7324 from: 1 to: 1239
KIBB7694.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1239
Size: 1034 205
AleI CACnn'nnGTG
Cuts at: 0 1216 1239
Size: 1216 23
AlwNI CAG_nnn'CTG
Cuts at: 0 1078 1123 1239
Size: 1078 45 116
Fragments arranged by size:
1078 116 45
ApaI G_GGCC'C
Cuts at: 0 496 1069 1105 1239
Size: 496 573 36 134
Fragments arranged by size:
573 496 134 36
ApaLI G'TGCA_C
Cuts at: 0 771 1239
Size: 771 468
AvaI C'yCGr_G
Cuts at: 0 147 1239
Size: 147 1092
BamHI G'GATC_C
Cuts at: 0 57 257 825 1239
Size: 57 200 568 414
Fragments arranged by size:
568 414 200 57
BanII G_rGCy'C
Cuts at: 0 41 271 308 427 496 535 955 1069
Size: 41 230 37 119 69 39 420 114
Cuts at: 1069 1105 1165 1177 1239
Size: 36 60 12 62
Fragments arranged by size:
420 230 119 114 69 62 60 41
39 37 36 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 415 1239
Size: 415 824
BbvCI CC'TCA_GC
Cuts at: 0 197 1239
Size: 197 1042
BglI GCCn_nnn'nGGC
Cuts at: 0 286 1239
Size: 286 953
BglII A'GATC_T
Cuts at: 0 366 1239
Size: 366 873
BlpI GC'TnA_GC
Cuts at: 0 421 1231 1239
Size: 421 810 8
Fragments arranged by size:
810 421 8
Bme1580I G_kGCm'C
Cuts at: 0 496 775 1069 1105 1239
Size: 496 279 294 36 134
Fragments arranged by size:
496 294 279 134 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 211 1072 1168 1239
Size: 42 169 861 96 71
Fragments arranged by size:
861 169 96 71 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 939 1239
Size: 418 521 300
Fragments arranged by size:
521 418 300
Bpu10I CC'TnA_GC
Cuts at: 0 197 1239
Size: 197 1042
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 618 648 1239
Size: 196 30 392 30 591
Fragments arranged by size:
591 392 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 338 1239
Size: 338 901
BseYI C'CCAG_C
Cuts at: 0 284 1012 1239
Size: 284 728 227
Fragments arranged by size:
728 284 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1239
Size: 434 805
BsiHKAI G_wGCw'C
Cuts at: 0 154 775 1239
Size: 154 621 464
Fragments arranged by size:
621 464 154
Bsp1286I G_dGCh'C
Cuts at: 0 41 154 271 308 427 496 535 775
Size: 41 113 117 37 119 69 39 240
Cuts at: 775 955 1069 1105 1165 1177 1239
Size: 180 114 36 60 12 62
Fragments arranged by size:
240 180 119 117 114 113 69 62
60 41 39 37 36 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 456 751 1239
Size: 456 295 488
Fragments arranged by size:
488 456 295
BsrBI CCG'CTC
Cuts at: 0 417 1230 1239
Size: 417 813 9
Fragments arranged by size:
813 417 9
BsrDI GCAATG_nn'
Cuts at: 0 235 843 1239
Size: 235 608 396
Fragments arranged by size:
608 396 235
BsrFI r'CCGG_y
Cuts at: 0 280 875 1239
Size: 280 595 364
Fragments arranged by size:
595 364 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 680 779 842 1239
Size: 680 99 63 397
Fragments arranged by size:
680 397 99 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 1239
Size: 396 843
BstYI r'GATC_y
Cuts at: 0 57 257 366 825 1239
Size: 57 200 109 459 414
Fragments arranged by size:
459 414 200 109 57
Bsu36I CC'TnA_GG
Cuts at: 0 113 1239
Size: 113 1126
BtgI C'CryG_G
Cuts at: 0 359 1169 1239
Size: 359 810 70
Fragments arranged by size:
810 359 70
BtsI GCAGTG_nn'
Cuts at: 0 140 451 1239
Size: 140 311 788
Fragments arranged by size:
788 311 140
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 552 587 1239
Size: 552 35 652
Fragments arranged by size:
652 552 35
DraIII CAC_nnn'GTG
Cuts at: 0 870 1239
Size: 870 369
EaeI y'GGCC_r
Cuts at: 0 877 1239
Size: 877 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 660 822 1239
Size: 660 162 417
Fragments arranged by size:
660 417 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 939 1034 1239
Size: 418 521 95 205
Fragments arranged by size:
521 418 205 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 186 1239
Size: 186 1053
EcoO109I rG'GnC_Cy
Cuts at: 0 20 29 445 492 493 716 1066 1102
Size: 20 9 416 47 1 223 350 36
Cuts at: 1102 1120 1189 1239
Size: 18 69 50
Fragments arranged by size:
416 350 223 69 50 47 36 20
18 9 1
HaeII r_GCGC'y
Cuts at: 0 741 1239
Size: 741 498
HindIII A'AGCT_T
Cuts at: 0 565 1239
Size: 565 674
MslI CAynn'nnrTG
Cuts at: 0 1216 1239
Size: 1216 23
MspA1I CmG'CkG
Cuts at: 0 163 169 991 1022 1239
Size: 163 6 822 31 217
Fragments arranged by size:
822 217 163 31 6
NaeI GCC'GGC
Cuts at: 0 282 1239
Size: 282 957
NdeI CA'TA_TG
Cuts at: 0 276 1239
Size: 276 963
NgoMIV G'CCGG_C
Cuts at: 0 280 1239
Size: 280 959
PasI CC'CwG_GG
Cuts at: 0 24 262 441 1239
Size: 24 238 179 798
Fragments arranged by size:
798 238 179 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 360 994 1098 1239
Size: 54 306 634 104 141
Fragments arranged by size:
634 306 141 104 54
PfoI T'CCnGG_A
Cuts at: 0 395 1239
Size: 395 844
PpuMI rG'GwC_Cy
Cuts at: 0 20 29 445 1239
Size: 20 9 416 794
Fragments arranged by size:
794 416 20 9
PspOMI G'GGCC_C
Cuts at: 0 492 1065 1101 1239
Size: 492 573 36 138
Fragments arranged by size:
573 492 138 36
PstI C_TGCA'G
Cuts at: 0 168 1239
Size: 168 1071
PvuII CAG'CTG
Cuts at: 0 169 991 1239
Size: 169 822 248
Fragments arranged by size:
822 248 169
SanDI GG'GwC_CC
Cuts at: 0 20 1239
Size: 20 1219
SexAI A'CCwGG_T
Cuts at: 0 866 1239
Size: 866 373
SfcI C'TryA_G
Cuts at: 0 164 186 204 1239
Size: 164 22 18 1035
Fragments arranged by size:
1035 164 22 18
StuI AGG'CCT
Cuts at: 0 202 1055 1097 1239
Size: 202 853 42 142
Fragments arranged by size:
853 202 142 42
StyI C'CwwG_G
Cuts at: 0 1092 1239
Size: 1092 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1182 1239
Size: 1182 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 817 849 1239
Size: 455 32 330 32 390
Fragments arranged by size:
455 390 330 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 281 1215 1239
Size: 281 934 24
Fragments arranged by size:
934 281 24
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApaLI AvaI BamHI BanII
BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII EaeI EciI Eco57MI EcoNI EcoO109I
HaeII HindIII MslI MspA1I NaeI NdeI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI DraI
DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NcoI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI