(Circular) (Six-base) MAPSORT of: pFN21AB7772.seq Check: 8240 from: 1 to: 6220 pFN21AB7772 6220 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3147 4030 278 Size: 53 83 186 936 1611 883 2468 Fragments arranged by size: 2468 1611 936 883 186 83 53 AccI GT'mk_AC Cuts at: 1937 3563 1937 Size: 1626 4594 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2850 3399 4283 5667 5935 1632 Size: 1218 549 884 1384 268 1917 Fragments arranged by size: 1917 1384 1218 884 549 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 5119 6096 1203 Size: 3916 977 1327 Fragments arranged by size: 3916 1327 977 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2630 1184 Size: 1446 4774 AlwNI CAG_nnn'CTG Cuts at: 2039 2837 3100 3963 5535 2039 Size: 798 263 863 1572 2724 Fragments arranged by size: 2724 1572 863 798 263 ApaI G_GGCC'C Cuts at: 2243 3060 2243 Size: 817 5403 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2023 3529 3755 3907 6016 1493 Size: 530 1506 226 152 2109 1697 Fragments arranged by size: 2109 1697 1506 530 226 152 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2147 3545 1870 Size: 73 204 1398 4545 Fragments arranged by size: 4545 1398 204 73 BamHI G'GATC_C Cuts at: 2008 3550 3917 2008 Size: 1542 367 4311 Fragments arranged by size: 4311 1542 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2678 3541 4996 618 Size: 325 217 627 891 863 1455 1842 Fragments arranged by size: 1842 1455 891 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2243 2696 2934 3060 3539 1391 Size: 852 453 238 126 479 4072 Fragments arranged by size: 4072 852 479 453 238 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2191 2225 4411 4445 1903 Size: 34 254 34 2186 34 3678 Fragments arranged by size: 3678 2186 254 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 2218 2700 Size: 107 122 71 631 605 364 182 482 Cuts at: 2700 4831 136 Size: 2131 1525 Fragments arranged by size: 2131 1525 631 605 482 364 182 122 107 71 BglII A'GATC_T Cuts at: 2161 2382 3415 6215 2161 Size: 221 1033 2800 2166 Fragments arranged by size: 2800 2166 1033 221 BlpI GC'TnA_GC Cuts at: 2443 3637 2443 Size: 1194 5026 Bme1580I G_kGCm'C Cuts at: 1790 2243 3060 3334 4281 5437 1790 Size: 453 817 274 947 1156 2573 Fragments arranged by size: 2573 1156 947 817 453 274 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3951 4909 442 Size: 416 53 83 399 24 2534 958 1753 Fragments arranged by size: 2534 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2209 2209 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3070 3122 3202 3252 3300 3461 4880 1239 Size: 1831 52 80 50 48 161 1419 2579 Fragments arranged by size: 2579 1831 1419 161 80 52 50 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2845 4345 5210 5508 2845 Size: 1500 865 298 3557 Fragments arranged by size: 3557 1500 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2978 4883 882 Size: 617 1479 1905 2219 Fragments arranged by size: 2219 1905 1479 617 BsaAI yAC'GTr Cuts at: 493 1204 4011 493 Size: 711 2807 2702 Fragments arranged by size: 2807 2702 711 BsaBI GATnn'nnATC Cuts at: 3414 6214 3414 Size: 2800 3420 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3144 3237 4027 4409 Size: 53 83 186 936 1611 93 790 382 Cuts at: 4409 5044 275 Size: 635 1451 Fragments arranged by size: 1611 1451 936 790 635 382 186 93 83 53 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3017 3047 1178 Size: 30 165 30 1614 30 4351 Fragments arranged by size: 4351 1614 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6220 BseYI C'CCAG_C Cuts at: 2244 3046 3199 3275 5423 5919 2244 Size: 802 153 76 2148 496 2545 Fragments arranged by size: 2545 2148 802 496 153 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 2468 1163 Size: 573 344 388 4915 Fragments arranged by size: 4915 573 388 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2235 3670 4431 5459 1540 Size: 275 186 234 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 234 186 BsiHKAI G_wGCw'C Cuts at: 3366 4281 4366 5437 3366 Size: 915 85 1071 4149 Fragments arranged by size: 4149 1071 915 85 BsmI GAATG_Cn' Cuts at: 2305 3405 3731 3824 2305 Size: 1100 326 93 4701 Fragments arranged by size: 4701 1100 326 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2696 2934 3060 3334 3366 3539 Size: 399 453 453 238 126 274 32 173 Cuts at: 3539 4281 4366 5437 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 453 399 274 238 173 126 85 32 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 3150 4109 5839 3150 Size: 959 1730 3531 Fragments arranged by size: 3531 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3575 844 Size: 2731 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2091 2565 4107 1394 Size: 429 268 474 1542 3507 Fragments arranged by size: 3507 1542 474 429 268 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2230 2519 3008 4864 5051 1700 Size: 110 420 289 489 1856 187 2869 Fragments arranged by size: 2869 1856 489 420 289 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 2730 794 Size: 1936 4284 BstBI TT'CG_AA Cuts at: 2070 5024 2070 Size: 2954 3266 BstEII G'GTnAC_C Cuts at: 1184 3004 1184 Size: 1820 4400 BstXI CCAn_nnnn'nTGG Cuts at: 3226 3283 3349 3226 Size: 57 66 6097 Fragments arranged by size: 6097 66 57 BstYI r'GATC_y Cuts at: 1972 2008 2059 2161 2382 3163 3409 3415 3514 Size: 36 51 102 221 781 246 6 99 Cuts at: 3514 3550 3917 4302 4319 5771 5857 5869 6215 Size: 36 367 385 17 1452 86 12 346 Cuts at: 6215 1972 Size: 1977 Fragments arranged by size: 1977 1452 781 385 367 346 246 221 102 99 86 51 36 36 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2550 3405 2550 Size: 855 5365 BtgI C'CryG_G Cuts at: 513 1065 2004 2595 3231 513 Size: 552 939 591 636 3502 Fragments arranged by size: 3502 939 636 591 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2806 3736 4530 4550 2806 Size: 930 794 20 4476 Fragments arranged by size: 4476 930 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2701 3228 Size: 54 1425 50 275 18 402 469 527 Cuts at: 3228 3667 4556 6185 8 Size: 439 889 1629 43 Fragments arranged by size: 1629 1425 889 527 469 439 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 3667 1537 Size: 275 420 1435 4090 Fragments arranged by size: 4090 1435 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2950 3195 4150 1353 Size: 1597 245 955 3423 Fragments arranged by size: 3423 1597 955 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 2864 4807 5181 5327 387 Size: 1064 138 867 408 1943 374 146 1280 Fragments arranged by size: 1943 1280 1064 867 408 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2850 3070 3122 3202 3252 3300 3399 Size: 393 1218 220 52 80 50 48 99 Cuts at: 3399 3461 4283 4880 5667 5935 1239 Size: 62 822 597 787 268 1524 Fragments arranged by size: 1524 1218 822 787 597 393 268 220 99 80 62 52 50 48 EcoNI CCTnn'n_nnAGG Cuts at: 2553 2553 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2239 2240 2273 3056 3057 3106 3272 2239 Size: 1 33 783 1 49 166 5187 Fragments arranged by size: 5187 783 166 49 33 1 1 EcoRI G'AATT_C Cuts at: 2023 3529 3907 2023 Size: 1506 378 4336 Fragments arranged by size: 4336 1506 378 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 2194 2355 5367 1678 Size: 516 161 3012 2531 Fragments arranged by size: 3012 2531 516 161 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2106 2138 4861 4893 4935 Size: 32 135 32 534 32 2723 32 42 Cuts at: 4935 4967 1373 Size: 32 2626 Fragments arranged by size: 2723 2626 534 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2711 3209 5334 5518 1305 Size: 1406 498 2125 184 2007 Fragments arranged by size: 2125 2007 1406 498 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3230 10 Size: 54 1425 343 1398 3000 Fragments arranged by size: 3000 1425 1398 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4178 4537 4696 518 Size: 648 3012 359 159 2042 Fragments arranged by size: 3012 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3636 4313 5461 5706 1606 Size: 2030 677 1148 245 2120 Fragments arranged by size: 2120 2030 1148 677 245 NaeI GCC'GGC Cuts at: 2232 2232 Size: 6220 NcoI C'CATG_G Cuts at: 513 1065 2004 2595 513 Size: 552 939 591 4138 Fragments arranged by size: 4138 939 591 552 NdeI CA'TA_TG Cuts at: 387 2320 387 Size: 1933 4287 NgoMIV G'CCGG_C Cuts at: 2230 2230 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3578 5123 1132 Size: 97 2349 1545 2229 Fragments arranged by size: 2349 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 3100 1108 Size: 1992 4228 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2273 2273 Size: 6220 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2239 3056 2239 Size: 817 5403 PstI C_TGCA'G Cuts at: 830 3572 830 Size: 2742 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 3025 3568 4703 5384 5575 826 Size: 219 1980 543 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 543 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 4324 5225 5487 820 Size: 197 926 881 1500 901 262 1553 Fragments arranged by size: 1553 1500 926 901 881 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2004 2595 3342 3659 513 Size: 552 939 591 747 317 3074 Fragments arranged by size: 3074 939 747 591 552 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2462 2494 2758 2790 6180 6212 2462 Size: 32 264 32 3390 32 2470 Fragments arranged by size: 3390 2470 264 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 2914 3412 3556 2914 Size: 498 144 5578 Fragments arranged by size: 5578 498 144 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2785 4349 2785 Size: 1564 4656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3145 4028 276 Size: 53 83 186 936 1611 883 2468 Fragments arranged by size: 2468 1611 936 883 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI