Restriction map for
pFN21AB7669
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstF5I BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HindIII
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 280 GTCGAC GT^[AC][GT]AC
AcyI 946 GGCGCC G[GA]^CG[CT]C
AfaI 897 GTAC GT^AC
AlwNI 253 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 607 GAGCTC G[AT]GC[AT]^C
AvaII 195 GGACC G^G[AT]CC
AvrII 424 CCTAGG C^CTAGG
BanI 946 GGCGCC G^G[CT][GA]CC
BbeI 946 GGCGCC GGCGC^C
BbsI 78 GTCTTC GAAGAC(2/6)
BlnI 424 CCTAGG C^CTAGG
BlpI 728 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 728 GCTCAGC GC^T[ACGT]AGC
BpuAI 78 GTCTTC GAAGAC(2/6)
BsaHI 946 GGCGCC G[GA]^CG[CT]C
BsgI 42 GTGCAG GTGCAG(16/14)
BsiHKAI 607 GAGCTC G[AT]GC[AT]^C
BsmBI 442 GAGACG CGTCTC(1/5)
BsmFI 243 GTCCC GGGAC(10/14)
BspMI 919 ACCTGC ACCTGC(4/8)
BstEII 171 GGTCACC G^GT[ACGT]ACC
BstPI 171 GGTCACC G^GT[ACGT]ACC
BstYI 668 AGATCC [GA]^GATC[CT]
CelII 728 GCTCAGC GC^T[ACGT]AGC
DraIII 845 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 171 GGTCACC G^GT[ACGT]ACC
HaeII 946 GGCGCC [GA]GCGC^[CT]
Hin1I 946 GGCGCC G[GA]^CG[CT]C
HpaI 886 GTTAAC GTT^AAC
KasI 946 GGCGCC G^GCGCC
MaeII 627 ACGT A^CGT
MflI 668 AGATCC [GA]^GATC[CT]
MseI 887 TTAA T^TAA
NaeI 943 GCCGGC GCC^GGC
NarI 946 GGCGCC GG^CGCC
NcoI 233 CCATGG C^CATGG
NgoMIV 943 GCCGGC G^CCGGC
NspI 293 ACATGC [GA]CATG^[CT]
PpuMI 194 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 756 CAGCTG CAG^CTG
RsaI 897 GTAC GT^AC
SacI 607 GAGCTC GAGCT^C
SalI 280 GTCGAC G^TCGAC
SfcI 333 CTACAG C^T[GA][CT]AG
SfoI 946 GGCGCC GGC^GCC
SgrAI 942 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 688 CCCGGG CCC^GGG
TaqI 281 TCGA T^CGA
Tru9I 887 TTAA T^TAA
TthHB8I 281 TCGA T^CGA
XhoII 668 AGATCC [GA]^GATC[CT]
XmaCI 688 CCCGGG C^CCGGG
XmaI 688 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 177 CGCG CG^CG
936 CGCG
AlwI 669 GATCC GGATC(4/5)
982 GATCC
ApaI 685 GGGCCC GGGCC^C
696 GGGCCC
BfaI 425 CTAG C^TAG
499 CTAG
BsiEI 275 CGGTCG CG[GA][CT]^CG
540 CGACCG
BstUI 177 CGCG CG^CG
936 CGCG
DdeI 729 CTCAG C^T[ACGT]AG
740 CTAAG
DraII 194 AGGACCT [GA]G^G[ACGT]CC[CT]
684 GGGGCCC
DsaI 167 CCACGG C^C[GA][CT]GG
233 CCATGG
EcoO109I 194 AGGACCT [GA]G^G[ACGT]CC[CT]
684 GGGGCCC
EcoT14I 233 CCATGG C^C[AT][AT]GG
424 CCTAGG
HgaI 444 GACGC GACGC(5/10)
811 GACGC
HincII 280 GTCGAC GT[CT]^[GA]AC
886 GTTAAC
HindII 280 GTCGAC GT[CT]^[GA]AC
886 GTTAAC
MaeI 425 CTAG C^TAG
499 CTAG
MaeIII 172 GTCAC ^GT[ACGT]AC
968 GTGAC
MvnI 177 CGCG CG^CG
936 CGCG
StyI 233 CCATGG C^C[AT][AT]GG
424 CCTAGG
Tsp45I 172 GTCAC ^GT[GC]AC
968 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DpnII
EarI EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TseI TspRI