(Circular) (Six-base) MAPSORT of: pFN21AB7669.seq Check: 1382 from: 1 to: 5719 pFN21AB7669 5719 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2933 2933 Size: 5719 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 3062 1937 Size: 349 776 4594 Fragments arranged by size: 4594 776 349 Acc65I G'GTAC_C Cuts at: 3040 3040 Size: 5719 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2743 3782 5166 5434 1632 Size: 1111 1039 1384 268 1917 Fragments arranged by size: 1917 1384 1111 1039 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 4618 5595 1203 Size: 3415 977 1327 Fragments arranged by size: 3415 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2533 1184 Size: 1349 4370 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2987 3019 2987 Size: 32 5687 AlwNI CAG_nnn'CTG Cuts at: 2263 3462 5034 2263 Size: 1199 1572 2948 Fragments arranged by size: 2948 1572 1199 ApaI G_GGCC'C Cuts at: 2694 2705 2694 Size: 11 5708 ApaLI G'TGCA_C Cuts at: 3776 4932 3776 Size: 1156 4563 ApoI r'AATT_y Cuts at: 1493 3028 3254 3406 5515 1493 Size: 1535 226 152 2109 1697 Fragments arranged by size: 2109 1697 1535 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 2144 2418 2439 2693 3044 1870 Size: 73 201 274 21 254 351 4545 Fragments arranged by size: 4545 351 274 254 201 73 21 AvrII C'CTAG_G Cuts at: 2429 2429 Size: 5719 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2951 3040 4495 618 Size: 325 217 627 1164 89 1455 1842 Fragments arranged by size: 1842 1455 1164 627 325 217 89 BanII G_rGCy'C Cuts at: 1391 2425 2616 2694 2705 2735 3038 1391 Size: 1034 191 78 11 30 303 4072 Fragments arranged by size: 4072 1034 303 191 78 30 11 BbeI G_GCGC'C Cuts at: 2955 2955 Size: 5719 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2076 928 Size: 971 177 4571 Fragments arranged by size: 4571 971 177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 2734 3136 2734 Size: 402 5317 Bme1580I G_kGCm'C Cuts at: 1790 2172 2694 2705 3780 4936 1790 Size: 382 522 11 1075 1156 2573 Fragments arranged by size: 2573 1156 1075 522 382 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2239 3450 4408 Size: 416 53 83 399 24 822 1211 958 Cuts at: 4408 442 Size: 1753 Fragments arranged by size: 1753 1211 958 822 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2537 2615 2657 4379 1239 Size: 950 348 78 42 1722 2579 Fragments arranged by size: 2579 1722 950 348 78 42 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 5719 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4709 5007 3844 Size: 865 298 4556 Fragments arranged by size: 4556 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2147 2428 2941 4382 882 Size: 617 648 281 513 1441 2219 Fragments arranged by size: 2219 1441 648 617 513 281 BsaAI yAC'GTr Cuts at: 493 1204 3510 493 Size: 711 2306 2702 Fragments arranged by size: 2702 2306 711 BsaBI GATnn'nnATC Cuts at: 5713 5713 Size: 5719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2952 3526 3908 4543 Size: 53 83 186 936 1419 574 382 635 Cuts at: 4543 275 Size: 1451 Fragments arranged by size: 1451 1419 936 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4152 4550 4824 4971 5382 4152 Size: 398 274 147 411 4489 Fragments arranged by size: 4489 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2024 2048 2054 2987 Size: 30 165 30 615 6 24 6 933 Cuts at: 2987 3017 1178 Size: 30 3880 Fragments arranged by size: 3880 933 615 165 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2459 2483 2999 1186 Size: 858 415 24 516 3906 Fragments arranged by size: 3906 858 516 415 24 BseYI C'CCAG_C Cuts at: 2271 2413 4922 5418 2271 Size: 142 2509 496 2572 Fragments arranged by size: 2572 2509 496 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2068 1163 Size: 573 332 4814 Fragments arranged by size: 4814 573 332 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2283 2548 3169 3930 4958 1540 Size: 275 186 282 265 621 761 1028 2301 Fragments arranged by size: 2301 1028 761 621 282 275 265 186 BsiHKAI G_wGCw'C Cuts at: 2616 3780 3865 4936 2616 Size: 1164 85 1071 3399 Fragments arranged by size: 3399 1164 1071 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 5719 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2425 2616 2694 2705 2735 3038 Size: 399 382 253 191 78 11 30 303 Cuts at: 3038 3780 3865 4936 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 303 253 191 85 78 30 11 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 3074 844 Size: 2089 141 3489 Fragments arranged by size: 3489 2089 141 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4214 4388 65 Size: 1183 2966 174 1396 Fragments arranged by size: 2966 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2526 2740 2948 4363 4550 1700 Size: 110 328 388 214 208 1415 187 2869 Fragments arranged by size: 2869 1415 388 328 214 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5719 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 2176 1184 Size: 992 4727 BstYI r'GATC_y Cuts at: 1972 2673 3049 3416 3801 3818 5270 5356 5368 Size: 701 376 367 385 17 1452 86 12 Cuts at: 5368 5714 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 701 385 376 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2172 2238 513 Size: 552 1107 66 3994 Fragments arranged by size: 3994 1107 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2350 2368 2977 3235 4029 4049 2350 Size: 18 609 258 794 20 4020 Fragments arranged by size: 4020 794 609 258 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 3023 3023 Size: 5719 DraIII CAC_nnn'GTG Cuts at: 2855 2855 Size: 5719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3166 4055 5684 Size: 54 1425 50 275 18 1336 889 1629 Cuts at: 5684 8 Size: 43 Fragments arranged by size: 1629 1425 1336 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2239 2460 2679 3649 1353 Size: 886 221 219 970 3423 Fragments arranged by size: 3423 970 886 221 219 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2364 2545 2573 2726 4306 4680 Size: 1064 138 775 181 28 153 1580 374 Cuts at: 4680 4826 387 Size: 146 1280 Fragments arranged by size: 1580 1280 1064 775 374 181 153 146 138 28 EcoICRI GAG'CTC Cuts at: 2614 2614 Size: 5719 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2537 2615 2657 2743 3782 4379 Size: 393 557 348 78 42 86 1039 597 Cuts at: 4379 5166 5434 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1039 787 597 557 393 348 268 86 78 42 EcoO109I rG'GnC_Cy Cuts at: 2200 2690 2200 Size: 490 5229 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 2955 4866 1678 Size: 1277 1911 2531 Fragments arranged by size: 2531 1911 1277 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2024 2050 2056 2582 Size: 32 135 32 446 6 26 6 526 Cuts at: 2582 2614 2824 2856 4360 4392 4434 4466 1373 Size: 32 210 32 1504 32 42 32 2626 Fragments arranged by size: 2626 1504 526 446 210 135 42 32 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2287 2893 3063 3309 669 Size: 988 281 349 606 170 246 3079 Fragments arranged by size: 3079 988 606 349 281 246 170 HindIII A'AGCT_T Cuts at: 748 748 Size: 5719 HpaI GTT'AAC Cuts at: 2893 3309 2893 Size: 416 5303 KasI G'GCGC_C Cuts at: 2951 2951 Size: 5719 KpnI G_GTAC'C Cuts at: 3044 3044 Size: 5719 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 2580 4833 5017 1305 Size: 1248 27 2253 184 2007 Fragments arranged by size: 2253 2007 1248 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3897 Fragments arranged by size: 3897 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3677 4036 4195 518 Size: 648 2511 359 159 2042 Fragments arranged by size: 2511 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2382 2707 2763 2967 3135 3812 4960 5205 Size: 776 325 56 204 168 677 1148 245 Cuts at: 5205 1606 Size: 2120 Fragments arranged by size: 2120 1148 776 677 325 245 204 168 56 NaeI GCC'GGC Cuts at: 2950 2950 Size: 5719 NarI GG'CG_CC Cuts at: 2952 2952 Size: 5719 NcoI C'CATG_G Cuts at: 513 1065 2238 513 Size: 552 1173 3994 Fragments arranged by size: 3994 1173 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NgoMIV G'CCGG_C Cuts at: 2948 2948 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 2302 3077 4622 1132 Size: 97 1073 775 1545 2229 Fragments arranged by size: 2229 1545 1073 775 97 PasI CC'CwG_GG Cuts at: 1348 2251 1348 Size: 903 4816 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2987 3019 3788 3820 5301 5333 2987 Size: 32 769 32 1481 32 3373 Fragments arranged by size: 3373 1481 769 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2200 Size: 5719 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2690 2701 2690 Size: 11 5708 PstI C_TGCA'G Cuts at: 830 3071 830 Size: 2241 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2763 2763 Size: 5719 SacI G_AGCT'C Cuts at: 2616 2616 Size: 5719 SalI G'TCGA_C Cuts at: 1936 2285 3061 1936 Size: 349 776 4594 Fragments arranged by size: 4594 776 349 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 3967 5679 3967 Size: 1712 4007 SfcI C'TryA_G Cuts at: 826 1045 2338 3067 4202 4883 5074 826 Size: 219 1293 729 1135 681 191 1471 Fragments arranged by size: 1471 1293 1135 729 681 219 191 SfoI GGC'GCC Cuts at: 2953 2953 Size: 5719 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 2948 1700 Size: 1248 4471 SmaI CCC'GGG Cuts at: 1872 2695 3046 1872 Size: 823 351 4545 Fragments arranged by size: 4545 823 351 SmlI C'TyrA_G Cuts at: 820 1017 1943 3823 4724 4986 820 Size: 197 926 1880 901 262 1553 Fragments arranged by size: 1880 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SrfI GCCC'GGGC Cuts at: 2695 2695 Size: 5719 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5719 StyI C'CwwG_G Cuts at: 513 1065 2238 2429 3158 513 Size: 552 1173 191 729 3074 Fragments arranged by size: 3074 1173 729 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2125 2558 2562 3746 3763 3916 4101 Size: 1255 545 433 4 1184 17 153 185 Cuts at: 4101 4546 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1184 545 445 433 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3965 5677 96 Size: 274 80 33 51 1443 1988 1712 138 Fragments arranged by size: 1988 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 5679 5711 2192 Size: 32 3455 32 2200 Fragments arranged by size: 3455 2200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5719 XbaI T'CTAG_A Cuts at: 3055 3055 Size: 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 2693 3044 1870 Size: 823 351 4545 Fragments arranged by size: 4545 823 351 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SwaI XcmI