Restriction map for
pFN21AB7669
(Linear) (Six-base) MAPSORT of: KIBB7669.seq Check: 2964 from: 1 to: 1014
KIBB7669.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 928 1014
Size: 928 86
AccI GT'mk_AC
Cuts at: 0 281 1014
Size: 281 733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1014
Size: 738 276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 1014
Size: 528 486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014
Size: 982 32
AlwNI CAG_nnn'CTG
Cuts at: 0 258 1014
Size: 258 756
ApaI G_GGCC'C
Cuts at: 0 689 700 1014
Size: 689 11 314
Fragments arranged by size:
689 314 11
AvaI C'yCGr_G
Cuts at: 0 139 413 434 688 1014
Size: 139 274 21 254 326
Fragments arranged by size:
326 274 254 139 21
AvrII C'CTAG_G
Cuts at: 0 424 1014
Size: 424 590
BanI G'GyrC_C
Cuts at: 0 946 1014
Size: 946 68
BanII G_rGCy'C
Cuts at: 0 420 611 689 700 730 1014
Size: 420 191 78 11 30 284
Fragments arranged by size:
420 284 191 78 30 11
BbeI G_GCGC'C
Cuts at: 0 950 1014
Size: 950 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 71 1014
Size: 71 943
BlpI GC'TnA_GC
Cuts at: 0 729 1014
Size: 729 285
Bme1580I G_kGCm'C
Cuts at: 0 167 689 700 1014
Size: 167 522 11 314
Fragments arranged by size:
522 314 167 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 234 1014
Size: 234 780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 532 610 652 1014
Size: 184 348 78 42 362
Fragments arranged by size:
362 348 184 78 42
Bpu10I CC'TnA_GC
Cuts at: 0 740 1014
Size: 740 274
BsaI GGTCTCn'nnnn_
Cuts at: 0 142 423 936 1014
Size: 142 281 513 78
Fragments arranged by size:
513 281 142 78
BsaHI Gr'CG_yC
Cuts at: 0 947 1014
Size: 947 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 19 43 49 982 1012 1014
Size: 13 6 24 6 933 30 2
Fragments arranged by size:
933 30 24 13 6 6 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 454 478 994 1014
Size: 39 415 24 516 20
Fragments arranged by size:
516 415 39 24 20
BseYI C'CCAG_C
Cuts at: 0 266 408 1014
Size: 266 142 606
Fragments arranged by size:
606 266 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 1014
Size: 63 951
BsiEI CG_ry'CG
Cuts at: 0 278 543 1014
Size: 278 265 471
Fragments arranged by size:
471 278 265
BsiHKAI G_wGCw'C
Cuts at: 0 611 1014
Size: 611 403
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 1014
Size: 436 578
Bsp1286I G_dGCh'C
Cuts at: 0 167 420 611 689 700 730 1014
Size: 167 253 191 78 11 30 284
Fragments arranged by size:
284 253 191 167 78 30 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 928 1014
Size: 928 86
BsrFI r'CCGG_y
Cuts at: 0 133 521 735 943 1014
Size: 133 388 214 208 71
Fragments arranged by size:
388 214 208 133 71
BstEII G'GTnAC_C
Cuts at: 0 171 1014
Size: 171 843
BstYI r'GATC_y
Cuts at: 0 668 1014
Size: 668 346
BtgI C'CryG_G
Cuts at: 0 167 233 1014
Size: 167 66 781
Fragments arranged by size:
781 167 66
BtsI GCAGTG_nn'
Cuts at: 0 345 363 972 1014
Size: 345 18 609 42
Fragments arranged by size:
609 345 42 18
DraIII CAC_nnn'GTG
Cuts at: 0 850 1014
Size: 850 164
EarI CTCTTCn'nnn_
Cuts at: 0 234 455 674 1014
Size: 234 221 219 340
Fragments arranged by size:
340 234 221 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 359 540 568 721 1014
Size: 359 181 28 153 293
Fragments arranged by size:
359 293 181 153 28
EcoICRI GAG'CTC
Cuts at: 0 609 1014
Size: 609 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 532 610 652 738 1014
Size: 184 348 78 42 86 276
Fragments arranged by size:
348 276 184 86 78 42
EcoO109I rG'GnC_Cy
Cuts at: 0 195 685 1014
Size: 195 490 329
Fragments arranged by size:
490 329 195
HaeII r_GCGC'y
Cuts at: 0 950 1014
Size: 950 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 19 45 51 577 609 819 851
Size: 13 6 26 6 526 32 210 32
Cuts at: 851 1014
Size: 163
Fragments arranged by size:
526 210 163 32 32 26 13 6
6
HincII GTy'rAC
Cuts at: 0 282 888 1014
Size: 282 606 126
Fragments arranged by size:
606 282 126
HpaI GTT'AAC
Cuts at: 0 888 1014
Size: 888 126
KasI G'GCGC_C
Cuts at: 0 946 1014
Size: 946 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 575 1014
Size: 548 27 439
Fragments arranged by size:
548 439 27
MspA1I CmG'CkG
Cuts at: 0 377 702 758 962 1014
Size: 377 325 56 204 52
Fragments arranged by size:
377 325 204 56 52
NaeI GCC'GGC
Cuts at: 0 945 1014
Size: 945 69
NarI GG'CG_CC
Cuts at: 0 947 1014
Size: 947 67
NcoI C'CATG_G
Cuts at: 0 233 1014
Size: 233 781
NgoMIV G'CCGG_C
Cuts at: 0 943 1014
Size: 943 71
NspI r_CATG'y
Cuts at: 0 297 1014
Size: 297 717
PasI CC'CwG_GG
Cuts at: 0 246 1014
Size: 246 768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 982 1014
Size: 982 32
PpuMI rG'GwC_Cy
Cuts at: 0 195 1014
Size: 195 819
PspOMI G'GGCC_C
Cuts at: 0 685 696 1014
Size: 685 11 318
Fragments arranged by size:
685 318 11
PvuII CAG'CTG
Cuts at: 0 758 1014
Size: 758 256
SacI G_AGCT'C
Cuts at: 0 611 1014
Size: 611 403
SalI G'TCGA_C
Cuts at: 0 280 1014
Size: 280 734
SfcI C'TryA_G
Cuts at: 0 333 1014
Size: 333 681
SfoI GGC'GCC
Cuts at: 0 948 1014
Size: 948 66
SgrAI Cr'CCGG_yG
Cuts at: 0 943 1014
Size: 943 71
SmaI CCC'GGG
Cuts at: 0 690 1014
Size: 690 324
SrfI GCCC'GGGC
Cuts at: 0 690 1014
Size: 690 324
StyI C'CwwG_G
Cuts at: 0 233 424 1014
Size: 233 191 590
Fragments arranged by size:
590 233 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 120 553 557 1014
Size: 120 433 4 457
Fragments arranged by size:
457 433 120 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 1014
Size: 187 32 795
Fragments arranged by size:
795 187 32
XmaI C'CCGG_G
Cuts at: 0 688 1014
Size: 688 326
Enzymes that do cut:
AarI AccI AcuI AlfI AloI AlwNI ApaI AvaI
AvrII BanI BanII BbeI BbsI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstEII BstYI
BtgI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII HpaI KasI MmeI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PpiI PpuMI PspOMI
PvuII SacI SalI SfcI SfoI SgrAI SmaI SrfI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII KpnI MfeI MluI MscI MslI
NdeI NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SanDI SapI SbfI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SphI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI