Restriction map for
pFN21AF0141
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccI AclI AcsI AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI AspI AvrII BamHI BcgI
BciVI BclI BglII BlnI BsaBI
BsiEI BsiWI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KspI MamI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 852 GGTACC G^GTACC
AcyI 718 GGCGCC G[GA]^CG[CT]C
AflII 1377 CTTAAG C^TTAAG
AflIII 3266 ACATGT A^C[GA][CT]GT
AhdI 3262 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1891 CCTGAGG CC^T[ACGT]AGG
Asp700I 1280 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 852 GGTACC G^GTACC
AspEI 3262 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3803 TGCGCA TGC^GCA
BbeI 718 GGCGCC GGCGC^C
BfrI 1377 CTTAAG C^TTAAG
BsaHI 718 GGCGCC G[GA]^CG[CT]C
BsmBI 3742 GAGACG CGTCTC(1/5)
BspHI 3950 TCATGA T^CATGA
BspLU11I 3266 ACATGT A^CATGT
BsrDI 299 GCAATG GCAATG(2/0)
BssSI 1961 CACGAG CACGAG(-5/-1)
BstXI 722 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1891 CCTGAGG CC^T[ACGT]AGG
Eam1105I 3262 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1891 CCTGAGG CC^T[ACGT]AGG
FseI 2475 GGCCGGCC GGCCGG^CC
FspI 3803 TGCGCA TGC^GCA
Hin1I 718 GGCGCC G[GA]^CG[CT]C
HincII 1118 GTCAAC GT[CT]^[GA]AC
HindII 1118 GTCAAC GT[CT]^[GA]AC
KasI 718 GGCGCC G^GCGCC
KpnI 852 GGTACC GGTAC^C
NarI 718 GGCGCC GG^CGCC
NheI 2139 GCTAGC G^CTAGC
RcaI 3950 TCATGA T^CATGA
SexAI 122 ACCTGGT A^CC[AT]GGT
SfiI 1339 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 718 GGCGCC GGC^GCC
SgrAI 1777 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2609 CCCGGG CCC^GGG
Tsp509I 3503 AATT ^AATT
XmaCI 2609 CCCGGG C^CCGGG
XmaI 2609 CCCGGG C^CCGGG
XmnI 1280 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 2492 TCCGGA T^CCGGA
3542 TCCGGA
BalI 1076 TGGCCA TGG^CCA
2543 TGGCCA
BbrPI 2176 CACGTG CAC^GTG
3610 CACGTG
BbsI 2712 GAAGAC GAAGAC(2/6)
3564 GAAGAC
BbvCI 3040 GCTGAGG CCTCAGC(-5/-2)
3931 GCTGAGG
BpuAI 2712 GAAGAC GAAGAC(2/6)
3564 GAAGAC
BsaAI 2176 CACGTG [CT]AC^GT[GA]
3610 CACGTG
BsaWI 2492 TCCGGA [AT]^CCGG[AT]
3542 TCCGGA
BseAI 2492 TCCGGA T^CCGGA
3542 TCCGGA
BsmI 2619 GAATGC GAATGC(1/-1)
1130 GCATTC
BspEI 2492 TCCGGA T^CCGGA
3542 TCCGGA
BstYI 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
EcoNI 2727 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3396 CCTGGAGGAGG
HgaI 4 GACGC GACGC(5/10)
1521 GACGC
MaeII 2177 ACGT A^CGT
3611 ACGT
MflI 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
MluNI 1076 TGGCCA TGG^CCA
2543 TGGCCA
MroI 2492 TCCGGA T^CCGGA
3542 TCCGGA
MscI 1076 TGGCCA TGG^CCA
2543 TGGCCA
NaeI 1340 GCCGGC GCC^GGC
2476 GCCGGC
NgoMIV 1340 GCCGGC G^CCGGC
2476 GCCGGC
NspI 359 GCATGT [GA]CATG^[CT]
3266 ACATGT
PmaCI 2176 CACGTG CAC^GTG
3610 CACGTG
PmlI 2176 CACGTG CAC^GTG
3610 CACGTG
TfiI 432 GATTC G^A[AT]TC
484 GAATC
XhoII 1434 AGATCC [GA]^GATC[CT]
2570 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI