Restriction map for
pFN21AB5050
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI BstAPI BstUI
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FauI FseI FspI
HaeII HhaI HinP1I HindIII KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 425 GACGCC G[GA]^CG[CT]C
AocI 877 CCTAAGG CC^T[ACGT]AGG
Asp700I 1723 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 408 TGGCCA TGG^CCA
BamHI 1338 GGATCC G^GATCC
BanI 1496 GGCACC G^G[CT][GA]CC
BbsI 706 GAAGAC GAAGAC(2/6)
BciVI 1412 GTATCC GTATCC(6/5)
BfaI 1738 CTAG C^TAG
BpuAI 706 GAAGAC GAAGAC(2/6)
BsaHI 425 GACGCC G[GA]^CG[CT]C
BseRI 860 GAGGAG GAGGAG(10/8)
BsgI 1811 GTGCAG GTGCAG(16/14)
BspHI 1167 TCATGA T^CATGA
BspMI 659 ACCTGC ACCTGC(4/8)
BsrBI 294 CCGCTC CCGCTC(-3/-3)
BsrFI 63 GCCGGT [GA]^CCGG[CT]
Bst1107I 1118 GTATAC GTA^TAC
BstBI 1730 TTCGAA TT^CGAA
BstEII 931 GGTAACC G^GT[ACGT]ACC
BstF5I 1458 GGATG GGATG(2/0)
BstPI 931 GGTAACC G^GT[ACGT]ACC
BstXI 1844 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1118 GTATAC GTA^TAC
Bsu36I 877 CCTAAGG CC^T[ACGT]AGG
Cac8I 510 GCTTGC GC[ACGT]^[ACGT]GC
Cfr10I 63 GCCGGT [GA]^CCGG[CT]
DraI 966 TTTAAA TTT^AAA
EaeI 408 TGGCCA [CT]^GGCC[GA]
EarI 739 GAAGAG CTCTTC(1/4)
Eco81I 877 CCTAAGG CC^T[ACGT]AGG
EcoNI 136 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 931 GGTAACC G^GT[ACGT]ACC
EcoRV 1579 GATATC GAT^ATC
EcoT22I 1159 ATGCAT ATGCA^T
FokI 1458 GGATG GGATG(9/13)
HgaI 425 GACGC GACGC(5/10)
Hin1I 425 GACGCC G[GA]^CG[CT]C
Ksp632I 739 GAAGAG CTCTTC(1/4)
MaeI 1738 CTAG C^TAG
MluNI 408 TGGCCA TGG^CCA
MscI 408 TGGCCA TGG^CCA
MspA1I 1272 CCGCTG C[AC]G^C[GT]G
NsiI 1159 ATGCAT ATGCA^T
NspV 1730 TTCGAA TT^CGAA
PaeR7I 1193 CTCGAG C^TCGAG
RcaI 1167 TCATGA T^CATGA
SexAI 789 ACCAGGT A^CC[AT]GGT
SfuI 1730 TTCGAA TT^CGAA
SmlI 1193 CTCGAG C^T[CT][GA]AG
SspI 1534 AATATT AAT^ATT
StuI 456 AGGCCT AGG^CCT
XcmI 1416 CCATGTGGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1193 CTCGAG C^TCGAG
XmnI 1723 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1118 GTATAC GT^[AC][GT]AC
1358 GTCTAC
AcsI 695 GAATTT [GA]^AATT[CT]
1723 GAATTT
AfaI 344 GTAC GT^AC
358 GTAC
ApoI 695 GAATTT [GA]^AATT[CT]
1723 GAATTT
AvaI 9 CCCGAG C^[CT]CG[GA]G
1193 CTCGAG
BclI 900 TGATCA T^GATCA
908 TGATCA
BmyI 186 GGGCAC G[AGT]GC[ACT]^C
1495 GGGCAC
BsoBI 9 CCCGAG C^[CT]CG[GA]G
1193 CTCGAG
Bsp1286I 186 GGGCAC G[AGT]GC[ACT]^C
1495 GGGCAC
BspLU11I 679 ACATGT A^CATGT
1242 ACATGT
DraII 389 GGGACCT [GA]G^G[ACGT]CC[CT]
1614 AGGTCCC
DsaI 279 CCATGG C^C[GA][CT]GG
808 CCATGG
EcoO109I 389 GGGACCT [GA]G^G[ACGT]CC[CT]
1614 AGGTCCC
FbaI 900 TGATCA T^GATCA
908 TGATCA
HapII 64 CCGG C^CGG
174 CCGG
HincII 1488 GTTAAC GT[CT]^[GA]AC
1506 GTTAAC
HindII 1488 GTTAAC GT[CT]^[GA]AC
1506 GTTAAC
HpaI 1488 GTTAAC GTT^AAC
1506 GTTAAC
HpaII 64 CCGG C^CGG
174 CCGG
HphI 1422 GGTGA GGTGA(8/7)
1720 GGTGA
MspI 64 CCGG C^CGG
174 CCGG
NcoI 279 CCATGG C^CATGG
808 CCATGG
NdeI 559 CATATG CA^TATG
1313 CATATG
PleI 1388 GAGTC GAGTC(4/5)
1821 GAGTC
PpuMI 389 GGGACCT [GA]G^G[AT]CC[CT]
1614 AGGTCCC
RsaI 344 GTAC GT^AC
358 GTAC
TaqI 1194 TCGA T^CGA
1731 TCGA
TthHB8I 1194 TCGA T^CGA
1731 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AflIII AluI AlwI AlwNI
AvaII BbvI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsmI BsrDI
BsrI BssKI BstNI BstYI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
HaeIII HinfI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII