(Circular) (Six-base) MAPSORT of: pFN21AB5050.seq Check: 4533 from: 1 to: 6586 pFN21AB5050 6586 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4396 278 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3124 3364 3929 1937 Size: 1187 240 565 4594 Fragments arranged by size: 4594 1187 565 240 Acc65I G'GTAC_C Cuts at: 3907 3907 Size: 6586 AclI AA'CG_TT Cuts at: 1563 4713 5086 1563 Size: 3150 373 3063 Fragments arranged by size: 3150 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 2730 3861 4649 6033 6301 1632 Size: 593 505 1131 788 1384 268 1917 Fragments arranged by size: 1917 1384 1131 788 593 505 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 2684 3247 3369 3489 5485 6462 1203 Size: 1481 563 122 120 1996 977 1327 Fragments arranged by size: 1996 1481 1327 977 563 122 120 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3654 1184 Size: 2470 4116 AlwNI CAG_nnn'CTG Cuts at: 2167 3113 3353 4329 5901 2167 Size: 946 240 976 1572 2852 Fragments arranged by size: 2852 1572 976 946 240 ApaLI G'TGCA_C Cuts at: 4643 5799 4643 Size: 1156 5430 ApoI r'AATT_y Cuts at: 1493 2700 3728 3895 4121 4273 6382 1493 Size: 1207 1028 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 1207 1028 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 2014 3198 3911 1870 Size: 73 71 1184 713 4545 Fragments arranged by size: 4545 1184 713 73 71 BamHI G'GATC_C Cuts at: 3343 3916 4283 3343 Size: 573 367 5646 Fragments arranged by size: 5646 573 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3501 3907 5362 618 Size: 325 217 627 1714 406 1455 1842 Fragments arranged by size: 1842 1714 1455 627 406 325 217 BanII G_rGCy'C Cuts at: 1391 3905 1391 Size: 2514 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2718 928 Size: 971 819 4796 Fragments arranged by size: 4796 971 819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4777 4811 1903 Size: 34 2840 34 3678 Fragments arranged by size: 3678 2840 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3428 4478 5688 3428 Size: 1050 1210 4326 Fragments arranged by size: 4326 1210 1050 BclI T'GATC_A Cuts at: 1548 2905 2913 1548 Size: 1357 8 5221 Fragments arranged by size: 5221 1357 8 BfrBI ATG'CAT Cuts at: 3166 3166 Size: 6586 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5197 136 Size: 107 122 71 631 605 3525 1525 Fragments arranged by size: 3525 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6581 6581 Size: 6586 BlpI GC'TnA_GC Cuts at: 4003 4003 Size: 6586 Bme1580I G_kGCm'C Cuts at: 1790 2195 3504 4647 5803 1790 Size: 405 1309 1143 1156 2573 Fragments arranged by size: 2573 1309 1156 1143 405 BmgBI CAC'GTC Cuts at: 1318 2100 1318 Size: 782 5804 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2364 2581 4317 Size: 416 53 83 399 24 947 217 1736 Cuts at: 4317 5275 442 Size: 958 1753 Fragments arranged by size: 1753 1736 958 947 416 399 217 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2888 3453 2317 Size: 571 565 5450 Fragments arranged by size: 5450 571 565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2515 2716 2746 2878 3135 5246 1239 Size: 1211 65 201 30 132 257 2111 2579 Fragments arranged by size: 2579 2111 1211 257 201 132 65 30 Bpu10I CC'TnA_GC Cuts at: 2105 2105 Size: 6586 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4711 5576 5874 4711 Size: 865 298 5423 Fragments arranged by size: 5423 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5249 882 Size: 617 3750 2219 Fragments arranged by size: 3750 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4377 493 Size: 711 3173 2702 Fragments arranged by size: 3173 2702 711 BsaBI GATnn'nnATC Cuts at: 6580 6580 Size: 6586 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2431 4393 4775 5410 Size: 53 83 186 936 898 1962 382 635 Cuts at: 5410 275 Size: 1451 Fragments arranged by size: 1962 1451 936 898 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5019 5417 5691 5838 6249 5019 Size: 398 274 147 411 5356 Fragments arranged by size: 5356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6361 Fragments arranged by size: 6361 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2880 1186 Size: 1694 4892 BseYI C'CCAG_C Cuts at: 5789 6285 5789 Size: 496 6090 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3837 1163 Size: 573 2101 3912 Fragments arranged by size: 3912 2101 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4036 4797 5825 1540 Size: 275 186 2035 761 1028 2301 Fragments arranged by size: 2301 2035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4647 4732 5803 4647 Size: 85 1071 5430 Fragments arranged by size: 5430 1071 85 BsmI GAATG_Cn' Cuts at: 3109 3483 3562 4097 4190 3109 Size: 374 79 535 93 5505 Fragments arranged by size: 5505 535 374 93 79 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 3504 3905 4647 4732 5803 1391 Size: 399 405 1309 401 742 85 1071 2174 Fragments arranged by size: 2174 1309 1071 742 405 401 399 85 BspEI T'CCGG_A Cuts at: 6249 6249 Size: 6586 BspHI T'CATG_A Cuts at: 3172 4475 6205 3172 Size: 1303 1730 3553 Fragments arranged by size: 3553 1730 1303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2673 3941 844 Size: 1829 1268 3489 Fragments arranged by size: 3489 1829 1268 BsrBI CCG'CTC Cuts at: 1394 1823 2301 4473 1394 Size: 429 478 2172 3507 Fragments arranged by size: 3507 2172 478 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2609 2762 3611 5081 5255 65 Size: 1183 1361 153 849 1470 174 1396 Fragments arranged by size: 1470 1396 1361 1183 849 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 5230 5417 1700 Size: 110 258 3162 187 2869 Fragments arranged by size: 3162 2869 258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6586 BssSI C'ACGA_G Cuts at: 4646 5658 4646 Size: 1012 5574 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6586 BstBI TT'CG_AA Cuts at: 3736 5390 3736 Size: 1654 4932 BstEII G'GTnAC_C Cuts at: 1184 2936 1184 Size: 1752 4834 BstXI CCAn_nnnn'nTGG Cuts at: 3856 3856 Size: 6586 BstYI r'GATC_y Cuts at: 1972 3074 3343 3434 3916 4283 4668 4685 6137 Size: 1102 269 91 482 367 385 17 1452 Cuts at: 6137 6223 6235 6581 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1102 482 385 367 346 269 91 86 17 12 BstZ17I GTA'TAC Cuts at: 3125 3125 Size: 6586 Bsu36I CC'TnA_GG Cuts at: 2883 2883 Size: 6586 BtgI C'CryG_G Cuts at: 513 1065 2284 2813 513 Size: 552 1219 529 4286 Fragments arranged by size: 4286 1219 552 529 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5847 Fragments arranged by size: 5847 683 56 BtsI GCAGTG_nn' Cuts at: 4102 4896 4916 4102 Size: 794 20 5772 Fragments arranged by size: 5772 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2341 2376 529 Size: 35 1777 35 4739 Fragments arranged by size: 4739 1777 35 35 DraI TTT'AAA Cuts at: 2973 3890 2973 Size: 917 5669 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5593 809 Size: 507 4277 1802 Fragments arranged by size: 4277 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 4033 4922 Size: 54 1425 50 275 18 583 1620 889 Cuts at: 4922 6551 8 Size: 1629 43 Fragments arranged by size: 1629 1620 1425 889 583 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4033 1537 Size: 275 2221 4090 Fragments arranged by size: 4090 2221 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2739 4516 1353 Size: 1386 1777 3423 Fragments arranged by size: 3423 1777 1386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3799 5173 5547 5693 387 Size: 1064 138 2210 1374 374 146 1280 Fragments arranged by size: 2210 1374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2450 2515 2716 2730 2746 2878 Size: 393 593 225 65 201 14 16 132 Cuts at: 2878 3135 3861 4649 5246 6033 6301 1239 Size: 257 726 788 597 787 268 1524 Fragments arranged by size: 1524 788 787 726 597 593 393 268 257 225 201 132 65 16 14 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2145 Size: 6586 EcoO109I rG'GnC_Cy Cuts at: 2395 3620 2395 Size: 1225 5361 EcoRI G'AATT_C Cuts at: 3895 4273 3895 Size: 378 6208 EcoRV GAT'ATC Cuts at: 3586 3586 Size: 6586 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2735 2735 Size: 6586 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 5733 1678 Size: 4055 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3045 3077 5227 5259 5301 Size: 32 135 32 1473 32 2150 32 42 Cuts at: 5301 5333 1373 Size: 32 2626 Fragments arranged by size: 2626 2150 1473 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3513 3930 4176 669 Size: 988 281 1557 18 417 246 3079 Fragments arranged by size: 3079 1557 988 417 281 246 18 HindIII A'AGCT_T Cuts at: 748 748 Size: 6586 HpaI GTT'AAC Cuts at: 3495 3513 4176 3495 Size: 18 663 5905 Fragments arranged by size: 5905 663 18 KpnI G_GTAC'C Cuts at: 3911 3911 Size: 6586 MfeI C'AATT_G Cuts at: 4185 4185 Size: 6586 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 3809 5700 5884 1305 Size: 954 1550 1891 184 2007 Fragments arranged by size: 2007 1891 1550 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2415 10 Size: 54 1425 343 583 4181 Fragments arranged by size: 4181 1425 583 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3027 3171 3636 4544 4903 5062 518 Size: 648 1861 144 465 908 359 159 2042 Fragments arranged by size: 2042 1861 908 648 465 359 159 144 MspA1I CmG'CkG Cuts at: 1606 3279 4002 4679 5827 6072 1606 Size: 1673 723 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 723 677 245 NcoI C'CATG_G Cuts at: 513 1065 2284 2813 513 Size: 552 1219 529 4286 Fragments arranged by size: 4286 1219 552 529 NdeI CA'TA_TG Cuts at: 387 2565 3319 387 Size: 2178 754 3654 Fragments arranged by size: 3654 2178 754 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NsiI A_TGCA'T Cuts at: 3168 3168 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 2113 2688 2922 3251 3944 5489 1132 Size: 97 884 575 234 329 693 1545 2229 Fragments arranged by size: 2229 1545 884 693 575 329 234 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6586 PciI A'CATG_T Cuts at: 2684 3247 5485 2684 Size: 563 2238 3785 Fragments arranged by size: 3785 2238 563 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6586 PfoI T'CCnGG_A Cuts at: 2466 2649 2466 Size: 183 6403 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4655 4687 6168 6200 4655 Size: 32 1481 32 5041 Fragments arranged by size: 5041 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2395 3620 2395 Size: 1225 5361 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6586 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PstI C_TGCA'G Cuts at: 830 3938 830 Size: 3108 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SbfI CC_TGCA'GG Cuts at: 3938 3938 Size: 6586 ScaI AGT'ACT Cuts at: 4834 6546 4834 Size: 1712 4874 SexAI A'CCwGG_T Cuts at: 2794 2794 Size: 6586 SfcI C'TryA_G Cuts at: 826 1045 2457 3078 3567 3934 5069 5750 5941 Size: 219 1412 621 489 367 1135 681 191 Cuts at: 5941 826 Size: 1471 Fragments arranged by size: 1471 1412 1135 681 621 489 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 3913 1872 Size: 2041 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3198 4690 5591 5853 820 Size: 197 926 1255 1492 901 262 1553 Fragments arranged by size: 1553 1492 1255 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4377 493 Size: 3884 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 3944 1132 Size: 2812 3774 SspI AAT'ATT Cuts at: 5 52 3541 4510 5 Size: 47 3489 969 2081 Fragments arranged by size: 3489 2081 969 47 StuI AGG'CCT Cuts at: 1517 2463 1517 Size: 946 5640 StyI C'CwwG_G Cuts at: 513 1065 2284 2416 2813 4025 513 Size: 552 1219 132 397 1212 3074 Fragments arranged by size: 3074 1219 1212 552 397 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4613 4630 4783 4968 5413 325 Size: 1255 3033 17 153 185 445 1498 Fragments arranged by size: 3033 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4832 6544 96 Size: 274 80 33 51 1443 2855 1712 138 Fragments arranged by size: 2855 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6546 6578 6546 Size: 32 6554 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6586 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3428 3428 Size: 6586 XhoI C'TCGA_G Cuts at: 1943 3198 1943 Size: 1255 5331 XmaI C'CCGG_G Cuts at: 1870 3911 1870 Size: 2041 4545 XmnI GAAnn'nnTTC Cuts at: 3732 4715 3732 Size: 983 5603 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4394 276 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI