(Circular) (Six-base) MAPSORT of: pFN21AB6358.seq Check: 243 from: 1 to: 5593 pFN21AB6358 5593 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2707 2707 Size: 5593 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3403 278 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2835 2936 1937 Size: 898 101 4594 Fragments arranged by size: 4594 898 101 Acc65I G'GTAC_C Cuts at: 2914 2914 Size: 5593 AclI AA'CG_TT Cuts at: 1563 3720 4093 1563 Size: 2157 373 3063 Fragments arranged by size: 3063 2157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3656 5040 5308 1632 Size: 2024 1384 268 1917 Fragments arranged by size: 2024 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5396 AflIII A'CryG_T Cuts at: 1203 4492 5469 1203 Size: 3289 977 1327 Fragments arranged by size: 3289 1327 977 AgeI A'CCGG_T Cuts at: 4424 4424 Size: 5593 AhdI GACnn_n'nnGTC Cuts at: 4322 4322 Size: 5593 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 1184 Size: 1206 4387 AlwNI CAG_nnn'CTG Cuts at: 3336 4908 3336 Size: 1572 4021 ApaI G_GGCC'C Cuts at: 2860 2860 Size: 5593 ApaLI G'TGCA_C Cuts at: 3650 4806 3650 Size: 1156 4437 ApoI r'AATT_y Cuts at: 1493 2902 3128 3280 5389 1493 Size: 1409 226 152 2109 1697 Fragments arranged by size: 2109 1697 1409 226 152 AseI AT'TA_AT Cuts at: 160 1032 4147 160 Size: 872 3115 1606 Fragments arranged by size: 3115 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2156 2300 2626 2859 2918 1870 Size: 73 99 114 144 326 233 59 4545 Fragments arranged by size: 4545 326 233 144 114 99 73 59 BamHI G'GATC_C Cuts at: 2923 3290 2923 Size: 367 5226 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 2296 2361 2914 4369 Size: 325 217 627 468 41 65 553 1455 Cuts at: 4369 618 Size: 1842 Fragments arranged by size: 1842 1455 627 553 468 325 217 65 41 BanII G_rGCy'C Cuts at: 1391 2661 2860 2912 1391 Size: 1270 199 52 4072 Fragments arranged by size: 4072 1270 199 52 BbeI G_GCGC'C Cuts at: 2259 2365 2259 Size: 106 5487 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4622 BbvCI CC'TCA_GC Cuts at: 2085 2562 2085 Size: 477 5116 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3784 3818 1903 Size: 34 1847 34 3678 Fragments arranged by size: 3678 1847 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 4695 3485 Size: 1210 4383 BclI T'GATC_A Cuts at: 1548 1548 Size: 5593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4204 136 Size: 107 122 71 631 605 2532 1525 Fragments arranged by size: 2532 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2760 5588 2760 Size: 2828 2765 BlpI GC'TnA_GC Cuts at: 3010 3010 Size: 5593 Bme1580I G_kGCm'C Cuts at: 1790 2301 2730 2860 3654 4810 1790 Size: 511 429 130 794 1156 2573 Fragments arranged by size: 2573 1156 794 511 429 130 BmgBI CAC'GTC Cuts at: 1318 2419 1318 Size: 1101 4492 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2581 3324 4282 Size: 416 53 83 399 24 1164 743 958 Cuts at: 4282 442 Size: 1753 Fragments arranged by size: 1753 1164 958 743 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5593 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2461 2481 4253 1239 Size: 1222 20 1772 2579 Fragments arranged by size: 2579 1772 1222 20 Bpu10I CC'TnA_GC Cuts at: 2085 2351 2562 2085 Size: 266 211 5116 Fragments arranged by size: 5116 266 211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 3718 4583 4881 2258 Size: 1460 865 298 2970 Fragments arranged by size: 2970 1460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4256 882 Size: 617 2757 2219 Fragments arranged by size: 2757 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3384 493 Size: 711 2180 2702 Fragments arranged by size: 2702 2180 711 BsaBI GATnn'nnATC Cuts at: 2759 5587 2759 Size: 2828 2765 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 2362 2793 3400 Size: 53 83 186 936 723 106 431 607 Cuts at: 3400 3782 4417 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 723 635 607 431 382 186 106 83 53 BsaWI w'CCGG_w Cuts at: 4026 4424 4698 4845 5256 4026 Size: 398 274 147 411 4363 Fragments arranged by size: 4363 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 1178 Size: 30 165 30 1095 30 4243 Fragments arranged by size: 4243 1095 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2075 2087 2102 2105 2129 2132 2330 Size: 874 15 12 15 3 24 3 198 Cuts at: 2330 2333 2465 2506 2591 1186 Size: 3 132 41 85 4188 Fragments arranged by size: 4188 874 198 132 85 41 24 15 15 12 3 3 3 BseYI C'CCAG_C Cuts at: 4796 5292 4796 Size: 496 5097 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 1163 Size: 573 492 4528 Fragments arranged by size: 4528 573 492 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2099 2130 2196 3043 3804 4832 Size: 275 186 98 31 66 847 761 1028 Cuts at: 4832 1540 Size: 2301 Fragments arranged by size: 2301 1028 847 761 275 186 98 66 31 BsiHKAI G_wGCw'C Cuts at: 2661 2685 2817 3654 3739 4810 2661 Size: 24 132 837 85 1071 3444 Fragments arranged by size: 3444 1071 837 132 85 24 BsmI GAATG_Cn' Cuts at: 3104 3197 3104 Size: 93 5500 BsmBI CGTCTCn'nnnn_ Cuts at: 2007 2007 Size: 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2661 2685 2730 2817 2860 2912 Size: 399 511 360 24 45 87 43 52 Cuts at: 2912 3654 3739 4810 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 511 399 360 87 85 52 45 43 24 BspEI T'CCGG_A Cuts at: 5256 5256 Size: 5593 BspHI T'CATG_A Cuts at: 3482 5212 3482 Size: 1730 3863 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2562 2707 2948 844 Size: 1718 145 241 3489 Fragments arranged by size: 3489 1718 241 145 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3480 1394 Size: 429 272 1385 3507 Fragments arranged by size: 3507 1385 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 4088 4262 65 Size: 1183 2840 174 1396 Fragments arranged by size: 2840 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 2599 4237 4424 1700 Size: 110 381 408 1638 187 2869 Fragments arranged by size: 2869 1638 408 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5593 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5593 BssSI C'ACGA_G Cuts at: 2684 3653 4665 2684 Size: 969 1012 3612 Fragments arranged by size: 3612 1012 969 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5593 BstBI TT'CG_AA Cuts at: 4397 4397 Size: 5593 BstEII G'GTnAC_C Cuts at: 1184 2018 2863 1184 Size: 834 845 3914 Fragments arranged by size: 3914 845 834 BstYI r'GATC_y Cuts at: 1972 2760 2923 3290 3675 3692 5144 5230 5242 Size: 788 163 367 385 17 1452 86 12 Cuts at: 5242 5588 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 788 385 367 346 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4854 Fragments arranged by size: 4854 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2730 3109 3903 3923 2186 Size: 544 379 794 20 3856 Fragments arranged by size: 3856 794 544 379 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5593 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5558 DraI TTT'AAA Cuts at: 2897 2897 Size: 5593 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4600 809 Size: 507 3284 1802 Fragments arranged by size: 3284 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2127 2181 Size: 54 1425 50 275 18 266 31 54 Cuts at: 2181 2193 2601 3040 3929 5558 8 Size: 12 408 439 889 1629 43 Fragments arranged by size: 1629 1425 889 439 408 275 266 54 54 50 43 31 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2096 2127 2193 3040 1537 Size: 275 284 31 66 847 4090 Fragments arranged by size: 4090 847 284 275 66 31 EarI CTCTTCn'nnn_ Cuts at: 1353 2388 3523 1353 Size: 1035 1135 3423 Fragments arranged by size: 3423 1135 1035 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4180 4554 4700 387 Size: 1064 138 2591 374 146 1280 Fragments arranged by size: 2591 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2659 2659 Size: 5593 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2461 2481 3656 4253 5040 5308 1239 Size: 393 829 20 1175 597 787 268 1524 Fragments arranged by size: 1524 1175 829 787 597 393 268 20 EcoO109I rG'GnC_Cy Cuts at: 2856 2856 Size: 5593 EcoRI G'AATT_C Cuts at: 2902 3280 2902 Size: 378 5215 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2524 2524 Size: 5593 FspI TGC'GCA Cuts at: 4099 4099 Size: 5593 HaeII r_GCGC'y Cuts at: 1678 2259 2365 4740 1678 Size: 581 106 2375 2531 Fragments arranged by size: 2531 2375 581 106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 4234 4266 4308 Size: 32 135 32 1037 32 1593 32 42 Cuts at: 4308 4340 1373 Size: 32 2626 Fragments arranged by size: 2626 1593 1037 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3183 669 Size: 988 281 999 246 3079 Fragments arranged by size: 3079 999 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5593 HpaI GTT'AAC Cuts at: 3183 3183 Size: 5593 KasI G'GCGC_C Cuts at: 2255 2361 2255 Size: 106 5487 KpnI G_GTAC'C Cuts at: 2918 2918 Size: 5593 MfeI C'AATT_G Cuts at: 3192 3192 Size: 5593 MluI A'CGCG_T Cuts at: 5469 5469 Size: 5593 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4707 4891 1305 Size: 3402 184 2007 Fragments arranged by size: 3402 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3771 Fragments arranged by size: 3771 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 3551 3910 4069 518 Size: 648 1443 942 359 159 2042 Fragments arranged by size: 2042 1443 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 2263 2712 3009 3686 4834 5079 1606 Size: 558 99 449 297 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 558 449 297 245 99 NaeI GCC'GGC Cuts at: 2193 2601 2193 Size: 408 5185 NarI GG'CG_CC Cuts at: 2256 2362 2256 Size: 106 5487 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4102 Fragments arranged by size: 4102 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5593 NgoMIV G'CCGG_C Cuts at: 2191 2599 2191 Size: 408 5185 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5593 NotI GC'GGCC_GC Cuts at: 2127 3040 2127 Size: 913 4680 NspI r_CATG'y Cuts at: 1132 1229 2951 4496 1132 Size: 97 1722 1545 2229 Fragments arranged by size: 2229 1722 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5593 PciI A'CATG_T Cuts at: 4492 4492 Size: 5593 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5593 PmeI GTTT'AAAC Cuts at: 2897 2897 Size: 5593 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3662 3694 5175 5207 3662 Size: 32 1481 32 4048 Fragments arranged by size: 4048 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5593 PsiI TTA'TAA Cuts at: 3163 3163 Size: 5593 PspOMI G'GGCC_C Cuts at: 2856 2856 Size: 5593 PstI C_TGCA'G Cuts at: 830 2559 2945 830 Size: 1729 386 3478 Fragments arranged by size: 3478 1729 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5593 SacI G_AGCT'C Cuts at: 2661 2661 Size: 5593 SalI G'TCGA_C Cuts at: 1936 2935 1936 Size: 999 4594 SbfI CC_TGCA'GG Cuts at: 2559 2945 2559 Size: 386 5207 ScaI AGT'ACT Cuts at: 2507 3841 5553 2507 Size: 1334 1712 2547 Fragments arranged by size: 2547 1712 1334 SfcI C'TryA_G Cuts at: 826 1045 2555 2941 4076 4757 4948 826 Size: 219 1510 386 1135 681 191 1471 Fragments arranged by size: 1510 1471 1135 681 386 219 191 SfoI GGC'GCC Cuts at: 2257 2363 2257 Size: 106 5487 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5593 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5593 SmaI CCC'GGG Cuts at: 1872 2861 2920 1872 Size: 989 59 4545 Fragments arranged by size: 4545 989 59 SmlI C'TyrA_G Cuts at: 820 1017 1943 2273 3697 4598 4860 820 Size: 197 926 330 1424 901 262 1553 Fragments arranged by size: 1553 1424 926 901 330 262 197 SnaBI TAC'GTA Cuts at: 493 3384 493 Size: 2891 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5593 SphI G_CATG'C Cuts at: 1132 2951 1132 Size: 1819 3774 SspI AAT'ATT Cuts at: 5 52 3517 5 Size: 47 3465 2081 Fragments arranged by size: 3465 2081 47 StuI AGG'CCT Cuts at: 1517 2213 1517 Size: 696 4897 StyI C'CwwG_G Cuts at: 513 1065 2004 3032 513 Size: 552 939 1028 3074 Fragments arranged by size: 3074 1028 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 3620 3637 3790 3975 4420 325 Size: 1255 918 1122 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1122 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 3839 5551 Size: 274 80 33 51 1443 528 1334 1712 Cuts at: 5551 96 Size: 138 Fragments arranged by size: 1712 1443 1334 528 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 5553 5585 2396 Size: 32 3125 32 2404 Fragments arranged by size: 3125 2404 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2831 1316 Size: 1515 4078 XbaI T'CTAG_A Cuts at: 2929 2929 Size: 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5593 XmaI C'CCGG_G Cuts at: 1870 2859 2918 1870 Size: 989 59 4545 Fragments arranged by size: 4545 989 59 XmnI GAAnn'nnTTC Cuts at: 2507 3722 2507 Size: 1215 4378 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3401 276 Size: 53 83 186 936 1867 2468 Fragments arranged by size: 2468 1867 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI