Restriction map for
pFN21AB6846
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbrPI BbsI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI ScaI SexAI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 122 GGTACC G^GTACC
AccI 511 GTCTAC GT^[AC][GT]AC
AcyI 79 GGCGCC G[GA]^CG[CT]C
Alw44I 7 GTGCAC G^TGCAC
ApaLI 7 GTGCAC G^TGCAC
Asp718I 122 GGTACC G^GTACC
AspHI 7 GTGCAC G[AT]GC[AT]^C
BbeI 79 GGCGCC GGCGC^C
BbvCI 455 CCTCAGC CCTCAGC(-5/-2)
BcgI 12 CGAATGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 528 CCGGG CC^[GC]GG
BglI 136 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 79 GGCGCC G[GA]^CG[CT]C
BsiHKAI 7 GTGCAC G[AT]GC[AT]^C
Bsp1407I 545 TGTACA T^GTACA
BsrGI 545 TGTACA T^GTACA
BsrI 70 CCAGT ACTGG(1/-1)
BstF5I 239 GGATG GGATG(2/0)
CfoI 80 GCGC GCG^C
DdeI 456 CTCAG C^T[ACGT]AG
DrdI 103 GACAGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 340 CCGTGG C^C[GA][CT]GG
EcoT14I 137 CCAAGG C^C[AT][AT]GG
FauI 306 GCGGG CCCGC(4/6)
FokI 239 GGATG GGATG(9/13)
HaeII 79 GGCGCC [GA]GCGC^[CT]
HapII 528 CCGG C^CGG
HhaI 80 GCGC GCG^C
Hin1I 79 GGCGCC G[GA]^CG[CT]C
HinP1I 80 GCGC G^CGC
HincII 325 GTCAAC GT[CT]^[GA]AC
HindII 325 GTCAAC GT[CT]^[GA]AC
HpaII 528 CCGG C^CGG
HphI 395 TCACC GGTGA(8/7)
KasI 79 GGCGCC G^GCGCC
KpnI 122 GGTACC GGTAC^C
MaeIII 101 GTGAC ^GT[ACGT]AC
MseI 218 TTAA T^TAA
MspI 528 CCGG C^CGG
NarI 79 GGCGCC GG^CGCC
NciI 528 CCGGG CC^[GC]GG
NlaIII 361 CATG CATG^
NspI 360 GCATGT [GA]CATG^[CT]
SbfI 560 CCTGCAGG CCTGCA^GG
SfaNI 448 GATGC GCATC(5/9)
SfiI 135 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 79 GGCGCC GGC^GCC
Sse8387I 560 CCTGCAGG CCTGCA^GG
SspBI 545 TGTACA T^GTACA
StuI 557 AGGCCT AGG^CCT
StyI 137 CCAAGG C^C[AT][AT]GG
Tru9I 218 TTAA T^TAA
Tsp45I 101 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 123 GTAC GT^AC
546 GTAC
AlwNI 274 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
313 CAGGCCCTG
BanI 79 GGCGCC G^G[CT][GA]CC
122 GGTACC
BpmI 443 CTCCAG CTGGAG(16/14)
115 CTGGAG
Cac8I 255 GCAGGC GC[ACGT]^[ACGT]GC
559 GCCTGC
DraII 275 AGGGCCT [GA]G^G[ACGT]CC[CT]
314 AGGCCCT
EcoNI 126 CCTCTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
230 CCTGGAAGAGG
EcoO109I 275 AGGGCCT [GA]G^G[ACGT]CC[CT]
314 AGGCCCT
HinfI 110 GAGTC G^A[ACGT]TC
509 GAGTC
MspA1I 355 CAGCTG C[AC]G^C[GT]G
458 CAGCTG
PleI 110 GAGTC GAGTC(4/5)
509 GAGTC
PvuII 355 CAGCTG CAG^CTG
458 CAGCTG
RsaI 123 GTAC GT^AC
546 GTAC
Tsp509I 220 AATT ^AATT
520 AATT
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cfr13I EarI EcoRII
Fnu4HI HaeIII ItaI Ksp632I MboII
MnlI MvaI MwoI NlaIV PstI
Sau96I ScrFI SfcI TseI TspRI