(Circular) (Six-base) MAPSORT of: pFN21AB7325.seq Check: 3436 from: 1 to: 9652 pFN21AB7325 9652 bp With 182 enzymes: SgfI * March 9, 2009 17:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7462 278 Size: 53 83 186 936 5926 2468 Fragments arranged by size: 5926 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2892 5673 5770 6995 1937 Size: 955 2781 97 1225 4594 Fragments arranged by size: 4594 2781 1225 955 97 Acc65I G'GTAC_C Cuts at: 6973 6973 Size: 9652 AclI AA'CG_TT Cuts at: 1563 2602 7779 8152 1563 Size: 1039 5177 373 3063 Fragments arranged by size: 5177 3063 1039 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2933 3681 3690 4245 4671 4768 4821 5180 Size: 1301 748 9 555 426 97 53 359 Cuts at: 5180 5561 6546 6560 6662 7715 9099 9367 1632 Size: 381 985 14 102 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1301 1053 985 748 555 426 381 359 268 102 97 53 14 9 AfeI AGC'GCT Cuts at: 4154 4154 Size: 9652 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9455 AflIII A'CryG_T Cuts at: 1203 5425 8551 9528 1203 Size: 4222 3126 977 1327 Fragments arranged by size: 4222 3126 1327 977 AgeI A'CCGG_T Cuts at: 2146 8483 2146 Size: 6337 3315 AhdI GACnn_n'nnGTC Cuts at: 8381 8381 Size: 9652 AleI CACnn'nnGTG Cuts at: 1166 3150 6353 6809 1166 Size: 1984 3203 456 4009 Fragments arranged by size: 4009 3203 1984 456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3379 1184 Size: 2195 7457 AlwNI CAG_nnn'CTG Cuts at: 2092 2485 2915 3232 4268 4456 4479 6472 6823 Size: 393 430 317 1036 188 23 1993 351 Cuts at: 6823 7395 8967 2092 Size: 572 1572 2777 Fragments arranged by size: 2777 1993 1572 1036 572 430 393 351 317 188 23 ApaI G_GGCC'C Cuts at: 2026 4026 2026 Size: 2000 7652 ApaLI G'TGCA_C Cuts at: 2843 4388 7709 8865 2843 Size: 1545 3321 1156 3630 Fragments arranged by size: 3630 3321 1545 1156 ApoI r'AATT_y Cuts at: 1493 2802 3041 5401 5732 5827 6723 6870 6961 Size: 1309 239 2360 331 95 896 147 91 Cuts at: 6961 7187 7339 9448 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2360 2109 1697 1309 896 331 239 226 152 147 95 91 AseI AT'TA_AT Cuts at: 160 1032 5834 8206 160 Size: 872 4802 2372 1606 Fragments arranged by size: 4802 2372 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9652 AvaI C'yCGr_G Cuts at: 1870 1943 2027 3390 4186 6977 1870 Size: 73 84 1363 796 2791 4545 Fragments arranged by size: 4545 2791 1363 796 84 73 BamHI G'GATC_C Cuts at: 5361 5646 6982 7349 5361 Size: 285 1336 367 7664 Fragments arranged by size: 7664 1336 367 285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3314 6973 8428 618 Size: 325 217 627 1527 3659 1455 1842 Fragments arranged by size: 3659 1842 1527 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2026 2160 2467 4026 4193 6971 1391 Size: 635 134 307 1559 167 2778 4072 Fragments arranged by size: 4072 2778 1559 635 307 167 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4228 4902 5745 928 Size: 971 2329 674 843 4835 Fragments arranged by size: 4835 2329 971 843 674 BbvCI CC'TCA_GC Cuts at: 3476 4118 3476 Size: 642 9010 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3191 3225 6506 6540 7843 7877 1903 Size: 34 1254 34 3281 34 1303 34 3678 Fragments arranged by size: 3678 3281 1303 1254 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7544 8754 7544 Size: 1210 8442 BclI T'GATC_A Cuts at: 1548 2830 5940 1548 Size: 1282 3110 5260 Fragments arranged by size: 5260 3110 1282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2096 3186 8263 Size: 107 122 71 631 605 424 1090 5077 Cuts at: 8263 136 Size: 1525 Fragments arranged by size: 5077 1525 1090 631 605 424 122 107 71 BglII A'GATC_T Cuts at: 5103 5809 9647 5103 Size: 706 3838 5108 Fragments arranged by size: 5108 3838 706 BlpI GC'TnA_GC Cuts at: 2592 3225 7069 2592 Size: 633 3844 5175 Fragments arranged by size: 5175 3844 633 Bme1580I G_kGCm'C Cuts at: 1790 2026 2847 4026 4168 4392 7713 8869 1790 Size: 236 821 1179 142 224 3321 1156 2573 Fragments arranged by size: 3321 2573 1179 1156 821 236 224 142 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9652 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4237 4710 Size: 416 53 83 399 24 2294 526 473 Cuts at: 4710 6070 7383 8341 442 Size: 1360 1313 958 1753 Fragments arranged by size: 2294 1753 1360 1313 958 526 473 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6617 1056 Size: 5561 4091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 4177 4501 5495 6069 6458 8312 1239 Size: 875 2063 324 994 574 389 1854 2579 Fragments arranged by size: 2579 2063 1854 994 875 574 389 324 Bpu10I CC'TnA_GC Cuts at: 2466 3176 3476 4118 2466 Size: 710 300 642 8000 Fragments arranged by size: 8000 710 642 300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2172 4976 7777 8642 8940 2172 Size: 2804 2801 865 298 2884 Fragments arranged by size: 2884 2804 2801 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3163 8315 882 Size: 617 1664 5152 2219 Fragments arranged by size: 5152 2219 1664 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 7443 493 Size: 711 1188 5051 2702 Fragments arranged by size: 5051 2702 1188 711 BsaBI GATnn'nnATC Cuts at: 9646 9646 Size: 9652 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 4004 4233 7459 Size: 53 83 186 936 1918 553 229 3226 Cuts at: 7459 7841 8476 275 Size: 382 635 1451 Fragments arranged by size: 3226 1918 1451 936 635 553 382 229 186 83 53 BsaWI w'CCGG_w Cuts at: 2050 2146 8085 8483 8757 8904 9315 2050 Size: 96 5939 398 274 147 411 2387 Fragments arranged by size: 5939 2387 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3362 3392 6576 6606 6614 Size: 30 165 30 1959 30 3184 30 8 Cuts at: 6614 6644 1178 Size: 30 4186 Fragments arranged by size: 4186 3184 1959 165 30 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2486 2722 5039 5728 5906 1186 Size: 1300 236 2317 689 178 4932 Fragments arranged by size: 4932 2317 1300 689 236 178 BseYI C'CCAG_C Cuts at: 2009 3594 3627 8855 9351 2009 Size: 1585 33 5228 496 2310 Fragments arranged by size: 5228 2310 1585 496 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 3153 3236 3845 3917 4032 5033 Size: 573 678 739 83 609 72 115 1001 Cuts at: 5033 5987 1163 Size: 954 4828 Fragments arranged by size: 4828 1001 954 739 678 609 573 115 83 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 2132 3989 7102 7863 8891 Size: 275 186 37 94 1857 3113 761 1028 Cuts at: 8891 1540 Size: 2301 Fragments arranged by size: 3113 2301 1857 1028 761 275 186 94 37 BsiHKAI G_wGCw'C Cuts at: 2847 3147 4142 4227 4392 4430 5298 7713 7798 Size: 300 995 85 165 38 868 2415 85 Cuts at: 7798 8869 2847 Size: 1071 3630 Fragments arranged by size: 3630 2415 1071 995 868 300 165 85 85 38 BsmI GAATG_Cn' Cuts at: 7163 7256 7163 Size: 93 9559 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2160 2467 2847 3147 4026 4142 Size: 399 236 134 307 380 300 879 116 Cuts at: 4142 4168 4193 4227 4392 4430 5298 6971 7713 Size: 26 25 34 165 38 868 1673 742 Cuts at: 7713 7798 8869 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1673 1071 879 868 742 399 380 307 300 236 165 134 116 85 38 34 26 25 BspEI T'CCGG_A Cuts at: 2050 9315 2050 Size: 7265 2387 BspHI T'CATG_A Cuts at: 2440 6340 7541 9271 2440 Size: 3900 1201 1730 2821 Fragments arranged by size: 3900 2821 1730 1201 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 3204 5364 6072 7007 844 Size: 1289 1071 2160 708 935 3489 Fragments arranged by size: 3489 2160 1289 1071 935 708 BsrBI CCG'CTC Cuts at: 1394 1823 3976 3985 7539 1394 Size: 429 2153 9 3554 3507 Fragments arranged by size: 3554 3507 2153 429 9 BsrDI GCAATG_nn' Cuts at: 65 1248 3099 4342 8147 8321 65 Size: 1183 1851 1243 3805 174 1396 Fragments arranged by size: 3805 1851 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 3708 3885 3988 4060 4181 8296 Size: 110 336 1562 177 103 72 121 4115 Cuts at: 8296 8483 1700 Size: 187 2869 Fragments arranged by size: 4115 2869 1562 336 187 177 121 110 103 72 BsrGI T'GTAC_A Cuts at: 96 6915 96 Size: 6819 2833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9652 BssSI C'ACGA_G Cuts at: 2078 3980 7712 8724 2078 Size: 1902 3732 1012 3006 Fragments arranged by size: 3732 3006 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5044 794 Size: 4250 5402 BstBI TT'CG_AA Cuts at: 8456 8456 Size: 9652 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9652 BstXI CCAn_nnnn'nTGG Cuts at: 3127 3127 Size: 9652 BstYI r'GATC_y Cuts at: 1972 4777 4845 4870 5103 5361 5646 5809 6736 Size: 2805 68 25 233 258 285 163 927 Cuts at: 6736 6982 7349 7734 7751 9203 9289 9301 9647 Size: 246 367 385 17 1452 86 12 346 Cuts at: 9647 1972 Size: 1977 Fragments arranged by size: 2805 1977 1452 927 385 367 346 285 258 246 233 163 86 68 25 17 12 Bsu36I CC'TnA_GG Cuts at: 2734 2734 Size: 9652 BtgI C'CryG_G Cuts at: 513 1065 2004 2017 4824 6580 513 Size: 552 939 13 2807 1756 3585 Fragments arranged by size: 3585 2807 1756 939 552 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4212 487 Size: 683 56 2986 5927 Fragments arranged by size: 5927 2986 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3861 3917 4713 5523 6111 6534 6718 7168 Size: 989 56 796 810 588 423 184 450 Cuts at: 7168 7962 7982 2872 Size: 794 20 4542 Fragments arranged by size: 4542 989 810 796 794 588 450 423 184 56 20 ClaI AT'CG_AT Cuts at: 1554 5133 5452 1554 Size: 3579 319 5754 Fragments arranged by size: 5754 3579 319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5361 5396 6100 6135 529 Size: 35 4797 35 704 35 4046 Fragments arranged by size: 4797 4046 704 35 35 35 DraI TTT'AAA Cuts at: 5230 5406 6956 5230 Size: 176 1550 7926 Fragments arranged by size: 7926 1550 176 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3922 8659 809 Size: 507 2606 4737 1802 Fragments arranged by size: 4737 2606 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2129 2343 Size: 54 1425 50 275 18 205 94 214 Cuts at: 2343 3879 3986 4029 4158 6217 7099 7988 9617 Size: 1536 107 43 129 2059 882 889 1629 Cuts at: 9617 8 Size: 43 Fragments arranged by size: 2059 1629 1536 1425 889 882 275 214 205 129 107 94 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 2129 3986 7099 1537 Size: 275 223 94 1857 3113 4090 Fragments arranged by size: 4090 3113 1857 275 223 94 EarI CTCTTCn'nnn_ Cuts at: 1353 2502 2550 2562 4418 4787 7582 1353 Size: 1149 48 12 1856 369 2795 3423 Fragments arranged by size: 3423 2795 1856 1149 369 48 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8239 8613 8759 387 Size: 1064 138 6650 374 146 1280 Fragments arranged by size: 6650 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2933 3681 3690 4177 4245 4501 Size: 393 482 819 748 9 487 68 256 Cuts at: 4501 4671 4768 4821 5180 5495 5561 6069 6458 Size: 170 97 53 359 315 66 508 389 Cuts at: 6458 6546 6560 6662 7715 8312 9099 9367 1239 Size: 88 14 102 1053 597 787 268 1524 Fragments arranged by size: 1524 1053 819 787 748 597 508 487 482 393 389 359 315 268 256 170 102 97 88 68 66 53 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 2737 4031 5891 2737 Size: 1294 1860 6498 Fragments arranged by size: 6498 1860 1294 EcoO109I rG'GnC_Cy Cuts at: 2022 2023 2089 2650 2944 3188 3277 4023 2022 Size: 1 66 561 294 244 89 746 7651 Fragments arranged by size: 7651 746 561 294 244 89 66 1 EcoRI G'AATT_C Cuts at: 3041 6961 7339 3041 Size: 3920 378 5354 Fragments arranged by size: 5354 3920 378 EcoRV GAT'ATC Cuts at: 6147 6147 Size: 9652 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5185 5893 5185 Size: 708 8944 FspI TGC'GCA Cuts at: 8158 8158 Size: 9652 HaeII r_GCGC'y Cuts at: 1678 3352 4156 6652 8799 1678 Size: 1674 804 2496 2147 2531 Fragments arranged by size: 2531 2496 2147 1674 804 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2815 2847 3801 Size: 32 135 32 1052 32 159 32 954 Cuts at: 3801 3833 4455 4487 4606 4638 4801 4833 6131 Size: 32 622 32 119 32 163 32 1298 Cuts at: 6131 6163 6614 6646 8293 8325 8367 8399 1373 Size: 32 451 32 1647 32 42 32 2626 Fragments arranged by size: 2626 1647 1298 1052 954 622 451 163 159 135 119 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4561 5771 6996 7242 669 Size: 988 281 2623 1210 1225 246 3079 Fragments arranged by size: 3079 2623 1225 1210 988 281 246 HindIII A'AGCT_T Cuts at: 748 6510 748 Size: 5762 3890 HpaI GTT'AAC Cuts at: 7242 7242 Size: 9652 KpnI G_GTAC'C Cuts at: 6977 6977 Size: 9652 MfeI C'AATT_G Cuts at: 7251 7251 Size: 9652 MluI A'CGCG_T Cuts at: 9528 9528 Size: 9652 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 3816 5881 8766 8950 1305 Size: 1164 1347 2065 2885 184 2007 Fragments arranged by size: 2885 2065 2007 1347 1164 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2345 4031 6219 10 Size: 54 1425 343 513 1686 2188 3443 Fragments arranged by size: 3443 2188 1686 1425 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3072 3125 3137 3150 3197 3821 4359 Size: 648 1906 53 12 13 47 624 538 Cuts at: 4359 4406 5093 5205 6353 6775 6809 7610 7969 Size: 47 687 112 1148 422 34 801 359 Cuts at: 7969 8128 518 Size: 159 2042 Fragments arranged by size: 2042 1906 1148 801 687 648 624 538 422 359 159 112 53 47 47 34 13 12 MspA1I CmG'CkG Cuts at: 1606 3533 5328 6047 6820 7068 7745 8893 9138 Size: 1927 1795 719 773 248 677 1148 245 Cuts at: 9138 1606 Size: 2120 Fragments arranged by size: 2120 1927 1795 1148 773 719 677 248 245 NaeI GCC'GGC Cuts at: 3887 3990 4062 3887 Size: 103 72 9477 Fragments arranged by size: 9477 103 72 NcoI C'CATG_G Cuts at: 513 1065 2004 4824 6580 513 Size: 552 939 2820 1756 3585 Fragments arranged by size: 3585 2820 1756 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9652 NgoMIV G'CCGG_C Cuts at: 3885 3988 4060 3885 Size: 103 72 9477 Fragments arranged by size: 9477 103 72 NheI G'CTAG_C Cuts at: 1052 6613 1052 Size: 5561 4091 NotI GC'GGCC_GC Cuts at: 7099 7099 Size: 9652 NspI r_CATG'y Cuts at: 1132 1229 4919 5204 7010 8555 1132 Size: 97 3690 285 1806 1545 2229 Fragments arranged by size: 3690 2229 1806 1545 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9652 PciI A'CATG_T Cuts at: 8551 8551 Size: 9652 PflMI CCAn_nnn'nTGG Cuts at: 1108 4774 5998 1108 Size: 3666 1224 4762 Fragments arranged by size: 4762 3666 1224 PmeI GTTT'AAAC Cuts at: 6956 6956 Size: 9652 PmlI CAC'GTG Cuts at: 2392 2392 Size: 9652 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7721 7753 9234 9266 7721 Size: 32 1481 32 8107 Fragments arranged by size: 8107 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2650 2944 3277 2650 Size: 294 333 9025 Fragments arranged by size: 9025 333 294 PshAI GACnn'nnGTC Cuts at: 1613 6482 1613 Size: 4869 4783 PsiI TTA'TAA Cuts at: 3423 3620 6876 7222 3423 Size: 197 3256 346 5853 Fragments arranged by size: 5853 3256 346 197 PspOMI G'GGCC_C Cuts at: 2022 4022 2022 Size: 2000 7652 PstI C_TGCA'G Cuts at: 830 6335 6468 7004 830 Size: 5505 133 536 3478 Fragments arranged by size: 5505 3478 536 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9652 PvuII CAG'CTG Cuts at: 3533 6047 6820 3533 Size: 2514 773 6365 Fragments arranged by size: 6365 2514 773 RsrII CG'GwC_CG Cuts at: 2144 2144 Size: 9652 SalI G'TCGA_C Cuts at: 1936 5769 6994 1936 Size: 3833 1225 4594 Fragments arranged by size: 4594 3833 1225 SapI GCTCTTCn'nnn_ Cuts at: 2502 4418 2502 Size: 1916 7736 SbfI CC_TGCA'GG Cuts at: 7004 7004 Size: 9652 ScaI AGT'ACT Cuts at: 4415 6329 7900 9612 4415 Size: 1914 1571 1712 4455 Fragments arranged by size: 4455 1914 1712 1571 SfcI C'TryA_G Cuts at: 826 1045 2084 3334 6135 6331 6464 6922 6937 Size: 219 1039 1250 2801 196 133 458 15 Cuts at: 6937 7000 8135 8816 9007 826 Size: 63 1135 681 191 1471 Fragments arranged by size: 2801 1471 1250 1135 1039 681 458 219 196 191 133 63 15 SfiI GGCCn_nnn'nGGCC Cuts at: 2096 2096 Size: 9652 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9652 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9652 SmaI CCC'GGG Cuts at: 1872 2029 4188 6979 1872 Size: 157 2159 2791 4545 Fragments arranged by size: 4545 2791 2159 157 SmlI C'TyrA_G Cuts at: 820 1017 1943 2187 4991 7756 8657 8919 820 Size: 197 926 244 2804 2765 901 262 1553 Fragments arranged by size: 2804 2765 1553 926 901 262 244 197 SnaBI TAC'GTA Cuts at: 493 7443 493 Size: 6950 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9652 SphI G_CATG'C Cuts at: 1132 4919 7010 1132 Size: 3787 2091 3774 Fragments arranged by size: 3787 3774 2091 SrfI GCCC'GGGC Cuts at: 4188 4188 Size: 9652 SspI AAT'ATT Cuts at: 5 52 6756 7576 5 Size: 47 6704 820 2081 Fragments arranged by size: 6704 2081 820 47 StuI AGG'CCT Cuts at: 1517 2193 3240 5081 1517 Size: 676 1047 1841 6088 Fragments arranged by size: 6088 1841 1047 676 StyI C'CwwG_G Cuts at: 513 1065 2004 3481 4032 4741 4824 6580 7091 Size: 552 939 1477 551 709 83 1756 511 Cuts at: 7091 513 Size: 3074 Fragments arranged by size: 3074 1756 1477 939 709 552 551 511 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4056 4126 7679 7696 7849 8034 8479 Size: 1255 2476 70 3553 17 153 185 445 Cuts at: 8479 325 Size: 1498 Fragments arranged by size: 3553 2476 1498 1255 445 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4413 5255 6327 Size: 274 80 33 51 1443 2436 842 1072 Cuts at: 6327 6460 6915 7898 9610 96 Size: 133 455 983 1712 138 Fragments arranged by size: 2436 1712 1443 1072 983 842 455 274 138 133 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6576 6608 9612 9644 6576 Size: 32 3004 32 6584 Fragments arranged by size: 6584 3004 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4469 1316 Size: 3153 6499 XbaI T'CTAG_A Cuts at: 6988 6988 Size: 9652 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5470 5470 Size: 9652 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9652 XmaI C'CCGG_G Cuts at: 1870 2027 4186 6977 1870 Size: 157 2159 2791 4545 Fragments arranged by size: 4545 2791 2159 157 XmnI GAAnn'nnTTC Cuts at: 7781 7781 Size: 9652 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7460 276 Size: 53 83 186 936 5926 2468 Fragments arranged by size: 5926 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI SacI SacII SanDI SexAI SfoI SwaI