Restriction map for
pFN21AB6344
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AocI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiEI Bsp1407I
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CpoI
DraI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MscI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccI 619 GTCTAC GT^[AC][GT]AC
AccII 676 CGCG CG^CG
AccIII 305 TCCGGA T^CCGGA
AcsI 547 AAATTC [GA]^AATT[CT]
AcyI 141 GGCGCC G[GA]^CG[CT]C
Alw44I 249 GTGCAC G^TGCAC
Aor51HI 374 AGCGCT AGC^GCT
ApaLI 249 GTGCAC G^TGCAC
ApoI 547 AAATTC [GA]^AATT[CT]
AspHI 249 GTGCAC G[AT]GC[AT]^C
AvaI 461 CCCGGG C^[CT]CG[GA]G
BanII 4 GGGCTC G[GA]GC[CT]^C
BbeI 141 GGCGCC GGCGC^C
BbsI 167 GTCTTC GAAGAC(2/6)
BfaI 110 CTAG C^TAG
BglI 19 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 167 GTCTTC GAAGAC(2/6)
BsaHI 141 GGCGCC G[GA]^CG[CT]C
BsaWI 305 TCCGGA [AT]^CCGG[AT]
BseAI 305 TCCGGA T^CCGGA
BseRI 133 CTCCTC GAGGAG(10/8)
BsgI 781 GTGCAG GTGCAG(16/14)
BsiHKAI 249 GTGCAC G[AT]GC[AT]^C
BsiWI 452 CGTACG C^GTACG
BsmBI 859 GAGACG CGTCTC(1/5)
BsmI 507 GAATGC GAATGC(1/-1)
BsoBI 461 CCCGGG C^[CT]CG[GA]G
BspDI 415 ATCGAT AT^CGAT
BspEI 305 TCCGGA T^CCGGA
BspHI 737 TCATGA T^CATGA
BsrBI 708 CCGCTC CCGCTC(-3/-3)
BsrFI 220 GCCGGC [GA]^CCGG[CT]
BsrI 849 ACTGG ACTGG(1/-1)
BstUI 676 CGCG CG^CG
BstYI 317 AGATCC [GA]^GATC[CT]
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
ClaI 415 ATCGAT AT^CGAT
DraII 299 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 499 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 610 GACCTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 436 CTCTTC CTCTTC(1/4)
Eco47III 374 AGCGCT AGC^GCT
EcoNI 805 CCTAACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 299 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 855 GCGGG CCCGC(4/6)
HgaI 756 GACGC GACGC(5/10)
Hin1I 141 GGCGCC G[GA]^CG[CT]C
HphI 494 TCACC GGTGA(8/7)
KasI 141 GGCGCC G^GCGCC
Ksp632I 436 CTCTTC CTCTTC(1/4)
MaeI 110 CTAG C^TAG
MaeII 500 ACGT A^CGT
MflI 317 AGATCC [GA]^GATC[CT]
MroI 305 TCCGGA T^CCGGA
MslI 412 CATATCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 676 CGCG CG^CG
NaeI 220 GCCGGC GCC^GGC
NarI 141 GGCGCC GG^CGCC
NgoMIV 220 GCCGGC G^CCGGC
PleI 283 GACTC GAGTC(4/5)
PpuMI 299 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 484 CAGCTG CAG^CTG
RcaI 737 TCATGA T^CATGA
ScaI 572 AGTACT AGT^ACT
SfoI 141 GGCGCC GGC^GCC
SmaI 461 CCCGGG CCC^GGG
TfiI 491 GAATC G^A[AT]TC
Tsp509I 548 AATT ^AATT
XhoII 317 AGATCC [GA]^GATC[CT]
XmaCI 461 CCCGGG C^CCGGG
XmaI 461 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 159 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
832 CAGGTTCTG
AvaII 300 GGACC G^G[AT]CC
387 GGTCC
BanI 141 GGCGCC G^G[CT][GA]CC
541 GGTGCC
BcnI 461 CCCGG CC^[GC]GG
462 CCGGG
BmyI 4 GGGCTC G[AGT]GC[ACT]^C
249 GTGCAC
BsmFI 464 GGGAC GGGAC(10/14)
564 GTCCC
Bsp1286I 4 GGGCTC G[AGT]GC[ACT]^C
249 GTGCAC
CfoI 142 GCGC GCG^C
375 GCGC
HaeII 141 GGCGCC [GA]GCGC^[CT]
374 AGCGCT
HhaI 142 GCGC GCG^C
375 GCGC
HinP1I 142 GCGC G^CGC
375 GCGC
HinfI 283 GACTC G^A[ACGT]TC
491 GAATC
MspA1I 484 CAGCTG C[AC]G^C[GT]G
512 CCGCTG
NciI 461 CCCGG CC^[GC]GG
462 CCGGG
NcoI 321 CCATGG C^CATGG
695 CCATGG
PflMI 273 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
831 CCAGGTTCTGG
Van91I 273 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
831 CCAGGTTCTGG
XcmI 431 CCAGCCTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
716 CCACACTTGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII ItaI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I