(Circular) (Six-base) MAPSORT of: pFN21AB6344.seq Check: 2460 from: 1 to: 5578 pFN21AB6344 5578 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2625 2921 1937 Size: 688 296 4594 Fragments arranged by size: 4594 688 296 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3641 5025 5293 1632 Size: 527 1482 1384 268 1917 Fragments arranged by size: 1917 1482 1384 527 268 AfeI AGC'GCT Cuts at: 2381 2381 Size: 5578 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5578 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AlwNI CAG_nnn'CTG Cuts at: 2169 2842 3321 4893 2169 Size: 673 479 1572 2854 Fragments arranged by size: 2854 1572 673 479 ApaLI G'TGCA_C Cuts at: 2254 3635 4791 2254 Size: 1381 1156 3041 Fragments arranged by size: 3041 1381 1156 ApoI r'AATT_y Cuts at: 1493 2552 2887 3113 3265 5374 1493 Size: 1059 335 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 335 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2466 2903 1870 Size: 73 523 437 4545 Fragments arranged by size: 4545 523 437 73 BamHI G'GATC_C Cuts at: 2908 3275 2908 Size: 367 5211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2546 2899 4354 618 Size: 325 217 627 359 400 353 1455 1842 Fragments arranged by size: 1842 1455 627 400 359 353 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2897 1391 Size: 622 884 4072 Fragments arranged by size: 4072 884 622 BbeI G_GCGC'C Cuts at: 2150 2150 Size: 5578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 928 Size: 971 266 4341 Fragments arranged by size: 4341 971 266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3769 3803 1903 Size: 34 1832 34 3678 Fragments arranged by size: 3678 1832 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 1548 Size: 5578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 4189 136 Size: 107 122 71 631 605 358 2159 1525 Fragments arranged by size: 2159 1525 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2995 2995 Size: 5578 Bme1580I G_kGCm'C Cuts at: 1790 2258 3639 4795 1790 Size: 468 1381 1156 2573 Fragments arranged by size: 2573 1381 1156 468 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5578 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2864 3309 4267 Size: 416 53 83 399 24 1447 445 958 Cuts at: 4267 442 Size: 1753 Fragments arranged by size: 1753 1447 958 445 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2678 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2594 2819 4238 1239 Size: 836 519 225 1419 2579 Fragments arranged by size: 2579 1419 836 519 225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3703 4568 4866 3703 Size: 865 298 4415 Fragments arranged by size: 4415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4241 882 Size: 617 2742 2219 Fragments arranged by size: 2742 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3369 493 Size: 711 2165 2702 Fragments arranged by size: 2702 2165 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 3385 3767 4402 Size: 53 83 186 936 614 1238 382 635 Cuts at: 4402 275 Size: 1451 Fragments arranged by size: 1451 1238 936 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2310 4011 4409 4683 4830 5241 2310 Size: 1701 398 274 147 411 2647 Fragments arranged by size: 2647 1701 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5353 Fragments arranged by size: 5353 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 1186 Size: 943 4635 BseYI C'CCAG_C Cuts at: 2097 2435 2491 4781 5277 2097 Size: 338 56 2290 496 2398 Fragments arranged by size: 2398 2290 496 338 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 1163 Size: 573 1071 3934 Fragments arranged by size: 3934 1071 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3789 4817 1540 Size: 275 186 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2258 3639 3724 4795 2258 Size: 1381 85 1071 3041 Fragments arranged by size: 3041 1381 1071 85 BsiWI C'GTAC_G Cuts at: 2457 2457 Size: 5578 BsmI GAATG_Cn' Cuts at: 2518 3089 3182 2518 Size: 571 93 4914 Fragments arranged by size: 4914 571 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2858 2858 Size: 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2258 2897 3639 3724 4795 1391 Size: 399 223 245 639 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 245 223 85 BspEI T'CCGG_A Cuts at: 2310 5241 2310 Size: 2931 2647 BspHI T'CATG_A Cuts at: 2742 3467 5197 2742 Size: 725 1730 3123 Fragments arranged by size: 3123 1730 725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 844 Size: 2089 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2715 3465 1394 Size: 429 892 750 3507 Fragments arranged by size: 3507 892 750 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4073 4247 65 Size: 1183 2825 174 1396 Fragments arranged by size: 2825 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2225 4222 4409 1700 Size: 110 415 1997 187 2869 Fragments arranged by size: 2869 1997 415 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5578 BssSI C'ACGA_G Cuts at: 3638 4650 3638 Size: 1012 4566 BstAPI GCAn_nnn'nTGC Cuts at: 794 2238 2383 2805 794 Size: 1444 145 422 3567 Fragments arranged by size: 3567 1444 422 145 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2322 2908 3275 3660 3677 5129 5215 5227 Size: 350 586 367 385 17 1452 86 12 Cuts at: 5227 5573 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 586 385 367 350 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2326 2560 2700 513 Size: 552 939 322 234 140 3391 Fragments arranged by size: 3391 939 552 322 234 140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 2291 3094 3888 3908 2291 Size: 803 794 20 3961 Fragments arranged by size: 3961 803 794 20 ClaI AT'CG_AT Cuts at: 1554 2421 1554 Size: 867 4711 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2509 2509 Size: 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4585 809 Size: 507 1305 1964 1802 Fragments arranged by size: 1964 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543 Size: 54 1425 50 275 18 1195 889 1629 Cuts at: 5543 8 Size: 43 Fragments arranged by size: 1629 1425 1195 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 3508 1353 Size: 1094 1061 3423 Fragments arranged by size: 3423 1094 1061 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2609 4165 4539 4685 387 Size: 1064 138 498 522 1556 374 146 1280 Fragments arranged by size: 1556 1280 1064 522 498 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2159 2594 2819 3641 4238 5025 Size: 393 443 84 435 225 822 597 787 Cuts at: 5025 5293 1239 Size: 268 1524 Fragments arranged by size: 1524 822 787 597 443 435 393 268 225 84 EcoNI CCTnn'n_nnAGG Cuts at: 2814 2814 Size: 5578 EcoO109I rG'GnC_Cy Cuts at: 2305 2305 Size: 5578 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2150 2383 4725 1678 Size: 472 233 2342 2531 Fragments arranged by size: 2531 2342 472 233 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4219 4251 4293 4325 1373 Size: 32 135 32 2647 32 42 32 2626 Fragments arranged by size: 2647 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2922 3168 669 Size: 988 281 984 246 3079 Fragments arranged by size: 3079 988 984 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KasI G'GCGC_C Cuts at: 2146 2146 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4692 4876 1305 Size: 3387 184 2007 Fragments arranged by size: 3387 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2421 3536 3895 4054 518 Size: 648 1255 1115 359 159 2042 Fragments arranged by size: 2042 1255 1115 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2491 2519 2994 3671 4819 5064 1606 Size: 885 28 475 677 1148 245 2120 Fragments arranged by size: 2120 1148 885 677 475 245 28 NaeI GCC'GGC Cuts at: 2227 2227 Size: 5578 NarI GG'CG_CC Cuts at: 2147 2147 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 2004 2326 2700 513 Size: 552 939 322 374 3391 Fragments arranged by size: 3391 939 552 374 322 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NgoMIV G'CCGG_C Cuts at: 2225 2225 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 2284 2842 1108 Size: 1176 558 3844 Fragments arranged by size: 3844 1176 558 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2305 Size: 5578 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PstI C_TGCA'G Cuts at: 830 2298 2379 2524 2930 830 Size: 1468 81 145 406 3478 Fragments arranged by size: 3478 1468 406 145 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 PvuII CAG'CTG Cuts at: 2491 2491 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2920 1936 Size: 984 4594 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 2579 3826 5538 2579 Size: 1247 1712 2619 Fragments arranged by size: 2619 1712 1247 SfcI C'TryA_G Cuts at: 826 1045 2294 2375 2520 2926 4061 4742 4933 Size: 219 1249 81 145 406 1135 681 191 Cuts at: 4933 826 Size: 1471 Fragments arranged by size: 1471 1249 1135 681 406 219 191 145 81 SfoI GGC'GCC Cuts at: 2148 2148 Size: 5578 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2468 2905 1872 Size: 596 437 4545 Fragments arranged by size: 4545 596 437 SmlI C'TyrA_G Cuts at: 820 1017 1943 3682 4583 4845 820 Size: 197 926 1739 901 262 1553 Fragments arranged by size: 1739 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2004 2326 2674 2700 3017 513 Size: 552 939 322 348 26 317 3074 Fragments arranged by size: 3074 939 552 348 322 317 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2516 3605 3622 3775 3960 4405 325 Size: 1255 936 1089 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 936 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 3824 5536 Size: 274 80 33 51 1443 600 1247 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1712 1443 1247 600 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5538 5570 5538 Size: 32 5546 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2728 2443 Size: 285 5293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2466 2903 1870 Size: 596 437 4545 Fragments arranged by size: 4545 596 437 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI