Restriction map for pFN21AB6344
 (Circular) (Six-base) MAPSORT of: pFN21AB6344.seq  Check: 2460  from: 1  to: 5578



pFN21AB6344  5578 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3388     278 
   Size:         53      83     186     936    1852    2468

  Fragments arranged by size: 

                2468    1852     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2625    2921    1937 
   Size:        688     296    4594

  Fragments arranged by size: 

                4594     688     296
 
Acc65I G'GTAC_C

Cuts at:   2899    2899 
   Size:       5578
 
AclI AA'CG_TT

Cuts at:   1563    3705    4078    1563 
   Size:       2142     373    3063

  Fragments arranged by size: 

                3063    2142     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2159    3641    5025    5293    1632 
   Size:        527    1482    1384     268    1917

  Fragments arranged by size: 

                1917    1482    1384     527     268
 
AfeI AGC'GCT

Cuts at:   2381    2381 
   Size:       5578
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5381
 
AflIII A'CryG_T

Cuts at:   1203    4477    5454    1203 
   Size:       3274     977    1327

  Fragments arranged by size: 

                3274    1327     977
 
AgeI A'CCGG_T

Cuts at:   4409    4409 
   Size:       5578
 
AhdI GACnn_n'nnGTC

Cuts at:   4307    4307 
   Size:       5578
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5578
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5578
 
AlwNI CAG_nnn'CTG

Cuts at:   2169    2842    3321    4893    2169 
   Size:        673     479    1572    2854

  Fragments arranged by size: 

                2854    1572     673     479
 
ApaLI G'TGCA_C

Cuts at:   2254    3635    4791    2254 
   Size:       1381    1156    3041

  Fragments arranged by size: 

                3041    1381    1156
 
ApoI r'AATT_y

Cuts at:   1493    2552    2887    3113    3265    5374    1493 
   Size:       1059     335     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1059     335     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4132     160 
   Size:        872    3100    1606

  Fragments arranged by size: 

                3100    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5578
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2466    2903    1870 
   Size:         73     523     437    4545

  Fragments arranged by size: 

                4545     523     437      73
 
BamHI G'GATC_C

Cuts at:   2908    3275    2908 
   Size:        367    5211
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2146    2546    2899    4354     618 
   Size:        325     217     627     359     400     353    1455    1842

  Fragments arranged by size: 

                1842    1455     627     400     359     353     325     217
            
 
BanII G_rGCy'C

Cuts at:   1391    2013    2897    1391 
   Size:        622     884    4072

  Fragments arranged by size: 

                4072     884     622
 
BbeI G_GCGC'C

Cuts at:   2150    2150 
   Size:       5578
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2165     928 
   Size:        971     266    4341

  Fragments arranged by size: 

                4341     971     266
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3769    3803    1903 
   Size:         34    1832      34    3678

  Fragments arranged by size: 

                3678    1832      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3470    4680    3470 
   Size:       1210    4368
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5578
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2030    4189     136 
   Size:        107     122      71     631     605     358    2159    1525

  Fragments arranged by size: 

                2159    1525     631     605     358     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5573    5573 
   Size:       5578
 
BlpI GC'TnA_GC

Cuts at:   2995    2995 
   Size:       5578
 
Bme1580I G_kGCm'C

Cuts at:   1790    2258    3639    4795    1790 
   Size:        468    1381    1156    2573

  Fragments arranged by size: 

                2573    1381    1156     468
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5578
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2864    3309    4267 
   Size:        416      53      83     399      24    1447     445     958

Cuts at:   4267     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1447     958     445     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5578
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2678    2678 
   Size:       5578
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2075    2594    2819    4238    1239 
   Size:        836     519     225    1419    2579

  Fragments arranged by size: 

                2579    1419     836     519     225
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3703    4568    4866    3703 
   Size:        865     298    4415

  Fragments arranged by size: 

                4415     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4241     882 
   Size:        617    2742    2219

  Fragments arranged by size: 

                2742    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3369     493 
   Size:        711    2165    2702

  Fragments arranged by size: 

                2702    2165     711
 
BsaBI GATnn'nnATC

Cuts at:   5572    5572 
   Size:       5578
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2147    3385    3767    4402 
   Size:         53      83     186     936     614    1238     382     635

Cuts at:   4402     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1238     936     635     614     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2310    4011    4409    4683    4830    5241    2310 
   Size:       1701     398     274     147     411    2647

  Fragments arranged by size: 

                2647    1701     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5353

  Fragments arranged by size: 

                5353     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2129    1186 
   Size:        943    4635
 
BseYI C'CCAG_C

Cuts at:   2097    2435    2491    4781    5277    2097 
   Size:        338      56    2290     496    2398

  Fragments arranged by size: 

                2398    2290     496     338      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2807    1163 
   Size:        573    1071    3934

  Fragments arranged by size: 

                3934    1071     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3028    3789    4817    1540 
   Size:        275     186    1027     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1027     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2258    3639    3724    4795    2258 
   Size:       1381      85    1071    3041

  Fragments arranged by size: 

                3041    1381    1071      85
 
BsiWI C'GTAC_G

Cuts at:   2457    2457 
   Size:       5578
 
BsmI GAATG_Cn'

Cuts at:   2518    3089    3182    2518 
   Size:        571      93    4914

  Fragments arranged by size: 

                4914     571      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2858    2858 
   Size:       5578
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2013    2258    2897    3639    3724    4795    1391 
   Size:        399     223     245     639     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     639     399     245     223      85
            
 
BspEI T'CCGG_A

Cuts at:   2310    5241    2310 
   Size:       2931    2647
 
BspHI T'CATG_A

Cuts at:   2742    3467    5197    2742 
   Size:        725    1730    3123

  Fragments arranged by size: 

                3123    1730     725
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2933     844 
   Size:       2089    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2715    3465    1394 
   Size:        429     892     750    3507

  Fragments arranged by size: 

                3507     892     750     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4073    4247      65 
   Size:       1183    2825     174    1396

  Fragments arranged by size: 

                2825    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2225    4222    4409    1700 
   Size:        110     415    1997     187    2869

  Fragments arranged by size: 

                2869    1997     415     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5578
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5578
 
BssSI C'ACGA_G

Cuts at:   3638    4650    3638 
   Size:       1012    4566
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2238    2383    2805     794 
   Size:       1444     145     422    3567

  Fragments arranged by size: 

                3567    1444     422     145
 
BstBI TT'CG_AA

Cuts at:   4382    4382 
   Size:       5578
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5578
 
BstYI r'GATC_y

Cuts at:   1972    2322    2908    3275    3660    3677    5129    5215    5227 
   Size:        350     586     367     385      17    1452      86      12

Cuts at:   5227    5573    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     586     385     367     350     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2326    2560    2700     513 
   Size:        552     939     322     234     140    3391

  Fragments arranged by size: 

                3391     939     552     322     234     140
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4839

  Fragments arranged by size: 

                4839     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2291    3094    3888    3908    2291 
   Size:        803     794      20    3961

  Fragments arranged by size: 

                3961     803     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2421    1554 
   Size:        867    4711
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5543
 
DraI TTT'AAA

Cuts at:   2882    2882 
   Size:       5578
 
DraIII CAC_nnn'GTG

Cuts at:   2509    2509 
   Size:       5578
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2621    4585     809 
   Size:        507    1305    1964    1802

  Fragments arranged by size: 

                1964    1802    1305     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3025    3914    5543 
   Size:         54    1425      50     275      18    1195     889    1629

Cuts at:   5543       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1195     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3025    1537 
   Size:        275    1213    4090

  Fragments arranged by size: 

                4090    1213     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2447    3508    1353 
   Size:       1094    1061    3423

  Fragments arranged by size: 

                3423    1094    1061
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2087    2609    4165    4539    4685     387 
   Size:       1064     138     498     522    1556     374     146    1280

  Fragments arranged by size: 

                1556    1280    1064     522     498     374     146     138
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2075    2159    2594    2819    3641    4238    5025 
   Size:        393     443      84     435     225     822     597     787

Cuts at:   5025    5293    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     822     787     597     443     435     393     268
                 225      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2814    2814 
   Size:       5578
 
EcoO109I rG'GnC_Cy

Cuts at:   2305    2305 
   Size:       5578
 
EcoRI G'AATT_C

Cuts at:   2887    3265    2887 
   Size:        378    5200
 
FspI TGC'GCA

Cuts at:   4084    4084 
   Size:       5578
 
HaeII r_GCGC'y

Cuts at:   1678    2150    2383    4725    1678 
   Size:        472     233    2342    2531

  Fragments arranged by size: 

                2531    2342     472     233
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4219    4251    4293    4325    1373 
   Size:         32     135      32    2647      32      42      32    2626

  Fragments arranged by size: 

                2647    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2922    3168     669 
   Size:        988     281     984     246    3079

  Fragments arranged by size: 

                3079     988     984     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5578
 
HpaI GTT'AAC

Cuts at:   3168    3168 
   Size:       5578
 
KasI G'GCGC_C

Cuts at:   2146    2146 
   Size:       5578
 
KpnI G_GTAC'C

Cuts at:   2903    2903 
   Size:       5578
 
MfeI C'AATT_G

Cuts at:   3177    3177 
   Size:       5578
 
MluI A'CGCG_T

Cuts at:   5454    5454 
   Size:       5578
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4692    4876    1305 
   Size:       3387     184    2007

  Fragments arranged by size: 

                3387    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3756

  Fragments arranged by size: 

                3756    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2421    3536    3895    4054     518 
   Size:        648    1255    1115     359     159    2042

  Fragments arranged by size: 

                2042    1255    1115     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2491    2519    2994    3671    4819    5064    1606 
   Size:        885      28     475     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     885     677     475     245      28
 
NaeI GCC'GGC

Cuts at:   2227    2227 
   Size:       5578
 
NarI GG'CG_CC

Cuts at:   2147    2147 
   Size:       5578
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2326    2700     513 
   Size:        552     939     322     374    3391

  Fragments arranged by size: 

                3391     939     552     374     322
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5578
 
NgoMIV G'CCGG_C

Cuts at:   2225    2225 
   Size:       5578
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5578
 
NotI GC'GGCC_GC

Cuts at:   3025    3025 
   Size:       5578
 
NspI r_CATG'y

Cuts at:   1132    1229    2936    4481    1132 
   Size:         97    1707    1545    2229

  Fragments arranged by size: 

                2229    1707    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5578
 
PciI A'CATG_T

Cuts at:   4477    4477 
   Size:       5578
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2284    2842    1108 
   Size:       1176     558    3844

  Fragments arranged by size: 

                3844    1176     558
 
PmeI GTTT'AAAC

Cuts at:   2882    2882 
   Size:       5578
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3647    3679    5160    5192    3647 
   Size:         32    1481      32    4033

  Fragments arranged by size: 

                4033    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2305    2305 
   Size:       5578
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5578
 
PsiI TTA'TAA

Cuts at:   3148    3148 
   Size:       5578
 
PstI C_TGCA'G

Cuts at:    830    2298    2379    2524    2930     830 
   Size:       1468      81     145     406    3478

  Fragments arranged by size: 

                3478    1468     406     145      81
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5578
 
PvuII CAG'CTG

Cuts at:   2491    2491 
   Size:       5578
 
SalI G'TCGA_C

Cuts at:   1936    2920    1936 
   Size:        984    4594
 
SbfI CC_TGCA'GG

Cuts at:   2930    2930 
   Size:       5578
 
ScaI AGT'ACT

Cuts at:   2579    3826    5538    2579 
   Size:       1247    1712    2619

  Fragments arranged by size: 

                2619    1712    1247
 
SfcI C'TryA_G

Cuts at:    826    1045    2294    2375    2520    2926    4061    4742    4933 
   Size:        219    1249      81     145     406    1135     681     191

Cuts at:   4933     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1249    1135     681     406     219     191     145
                  81
 
SfoI GGC'GCC

Cuts at:   2148    2148 
   Size:       5578
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5578
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5578
 
SmaI CCC'GGG

Cuts at:   1872    2468    2905    1872 
   Size:        596     437    4545

  Fragments arranged by size: 

                4545     596     437
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3682    4583    4845     820 
   Size:        197     926    1739     901     262    1553

  Fragments arranged by size: 

                1739    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3369     493 
   Size:       2876    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5578
 
SphI G_CATG'C

Cuts at:   1132    2936    1132 
   Size:       1804    3774
 
SspI AAT'ATT

Cuts at:      5      52    3502       5 
   Size:         47    3450    2081

  Fragments arranged by size: 

                3450    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5578
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2326    2674    2700    3017     513 
   Size:        552     939     322     348      26     317    3074

  Fragments arranged by size: 

                3074     939     552     348     322     317      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2516    3605    3622    3775    3960    4405     325 
   Size:       1255     936    1089      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1089     936     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2577    3824    5536 
   Size:        274      80      33      51    1443     600    1247    1712

Cuts at:   5536      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1247     600     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5538    5570    5538 
   Size:         32    5546
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5578
 
XbaI T'CTAG_A

Cuts at:   2914    2914 
   Size:       5578
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2443    2728    2443 
   Size:        285    5293
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5578
 
XmaI C'CCGG_G

Cuts at:   1870    2466    2903    1870 
   Size:        596     437    4545

  Fragments arranged by size: 

                4545     596     437
 
XmnI GAAnn'nnTTC

Cuts at:   3707    3707 
   Size:       5578
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3386     276 
   Size:         53      83     186     936    1852    2468

  Fragments arranged by size: 

                2468    1852     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI
   Bpu10I    BstXI  BstZ17I   Bsu36I  EcoICRI    EcoRV     FalI     FseI
    FspAI     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com