Restriction map for
pFN21AE0347
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SnaBI SpeI
SspBI SwaI Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 2020 GAATTT [GA]^AATT[CT]
AlwNI 2479 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1589 AGCGCT AGC^GCT
ApoI 2020 GAATTT [GA]^AATT[CT]
BalI 866 TGGCCA TGG^CCA
BbeI 1610 GGCGCC GGCGC^C
BglI 377 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 189 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 189 GCTAAGC GC^T[ACGT]AGC
BsaAI 1870 TACGTG [CT]AC^GT[GA]
BsaI 813 GGTCTC GGTCTC(1/5)
BspHI 2375 TCATGA T^CATGA
BsrDI 1830 CATTGC GCAATG(2/0)
BstAPI 1433 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2017 TTCGAA TT^CGAA
BstXI 783 CCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2420 GGATCT [GA]^GATC[CT]
CelII 189 GCTAAGC GC^T[ACGT]AGC
DraIII 1434 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 247 GACACCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1589 AGCGCT AGC^GCT
HincII 67 GTCAAC GT[CT]^[GA]AC
HindII 67 GTCAAC GT[CT]^[GA]AC
HindIII 1549 AAGCTT A^AGCTT
KasI 1610 GGCGCC G^GCGCC
MflI 2420 GGATCT [GA]^GATC[CT]
MluNI 866 TGGCCA TGG^CCA
MscI 866 TGGCCA TGG^CCA
NarI 1610 GGCGCC GG^CGCC
NspV 2017 TTCGAA TT^CGAA
PaeR7I 2260 CTCGAG C^TCGAG
PflMI 909 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2375 TCATGA T^CATGA
SacI 2410 GAGCTC GAGCT^C
SbfI 1635 CCTGCAGG CCTGCA^GG
SexAI 427 ACCAGGT A^CC[AT]GGT
SfiI 376 GGCCGGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1610 GGCGCC GGC^GCC
SfuI 2017 TTCGAA TT^CGAA
SgrAI 39 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2218 CCCGGG CCC^GGG
SphI 770 GCATGC GCATG^C
Sse8387I 1635 CCTGCAGG CCTGCA^GG
SspI 1903 AATATT AAT^ATT
Tsp45I 1655 GTGAC ^GT[GC]AC
Van91I 909 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2260 CTCGAG C^TCGAG
XhoII 2420 GGATCT [GA]^GATC[CT]
XmaCI 2218 CCCGGG C^CCGGG
XmaI 2218 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 158 CGCG CG^CG
1752 CGCG
Alw44I 2403 GTGCAC G^TGCAC
2437 GTGCAC
ApaLI 2403 GTGCAC G^TGCAC
2437 GTGCAC
BanI 1610 GGCGCC G^G[CT][GA]CC
2470 GGTGCC
BbsI 52 GAAGAC GAAGAC(2/6)
1139 GAAGAC
BpuAI 52 GAAGAC GAAGAC(2/6)
1139 GAAGAC
BsaBI 1234 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGGCTATC
BseRI 31 GAGGAG GAGGAG(10/8)
1822 GAGGAG
BsgI 1431 CTGCAC GTGCAG(16/14)
1091 GTGCAG
BsiEI 1538 CGGCCG CG[GA][CT]^CG
2068 CGGCCG
BsmBI 885 CGTCTC CGTCTC(1/5)
438 GAGACG
BspLU11I 1400 ACATGT A^CATGT
1574 ACATGT
BspMI 2030 ACCTGC ACCTGC(4/8)
609 GCAGGT
BstUI 158 CGCG CG^CG
1752 CGCG
EagI 1538 CGGCCG C^GGCCG
2068 CGGCCG
EclXI 1538 CGGCCG C^GGCCG
2068 CGGCCG
Eco52I 1538 CGGCCG C^GGCCG
2068 CGGCCG
EcoNI 570 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1989 CCTGTATGAGG
MaeIII 1571 GTTAC ^GT[ACGT]AC
1655 GTGAC
MamI 1234 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGGCTATC
MvnI 158 CGCG CG^CG
1752 CGCG
NcoI 469 CCATGG C^CATGG
722 CCATGG
NruI 157 TCGCGA TCG^CGA
1751 TCGCGA
SapI 267 GCTCTTC GCTCTTC(1/4)
2010 GCTCTTC
TfiI 1111 GAATC G^A[AT]TC
1971 GAATC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI ApaI AspHI AvaI AvaII
BanII BbvI BcgI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BssSI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I