(Circular) (Six-base) MAPSORT of: pFN21AE0347.seq Check: 9887 from: 1 to: 7264 pFN21AE0347 7264 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2605 4044 2605 Size: 1439 5825 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5074 278 Size: 53 83 186 936 3538 2468 Fragments arranged by size: 3538 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4607 1937 Size: 2670 4594 Acc65I G'GTAC_C Cuts at: 4585 4585 Size: 7264 AclI AA'CG_TT Cuts at: 1563 5391 5764 1563 Size: 3828 373 3063 Fragments arranged by size: 3828 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 3252 3542 3837 4562 5327 6711 6979 Size: 1379 241 290 295 725 765 1384 268 Cuts at: 6979 1632 Size: 1917 Fragments arranged by size: 1917 1384 1379 765 725 295 290 268 241 AfeI AGC'GCT Cuts at: 3596 3596 Size: 7264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7067 AflIII A'CryG_T Cuts at: 1203 3405 3579 4455 6163 7140 1203 Size: 2202 174 876 1708 977 1327 Fragments arranged by size: 2202 1708 1327 977 876 174 AgeI A'CCGG_T Cuts at: 6095 6095 Size: 7264 AhdI GACnn_n'nnGTC Cuts at: 5993 5993 Size: 7264 AleI CACnn'nnGTG Cuts at: 1166 4454 1166 Size: 3288 3976 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7264 AlwNI CAG_nnn'CTG Cuts at: 4489 5007 6579 4489 Size: 518 1572 5174 Fragments arranged by size: 5174 1572 518 ApaI G_GGCC'C Cuts at: 3013 3926 4201 3013 Size: 913 275 6076 Fragments arranged by size: 6076 913 275 ApaLI G'TGCA_C Cuts at: 4408 4442 5321 6477 4408 Size: 34 879 1156 5195 Fragments arranged by size: 5195 1156 879 34 ApoI r'AATT_y Cuts at: 1493 4025 4573 4799 4951 7060 1493 Size: 2532 548 226 152 2109 1697 Fragments arranged by size: 2532 2109 1697 548 226 152 AseI AT'TA_AT Cuts at: 160 1032 5818 160 Size: 872 4786 1606 Fragments arranged by size: 4786 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7264 AvaI C'yCGr_G Cuts at: 1870 1943 2376 4223 4265 4589 1870 Size: 73 433 1847 42 324 4545 Fragments arranged by size: 4545 1847 433 324 73 42 BamHI G'GATC_C Cuts at: 4594 4961 4594 Size: 367 6897 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3615 4475 4585 6040 618 Size: 325 217 627 1828 860 110 1455 1842 Fragments arranged by size: 1842 1828 1455 860 627 325 217 110 BanII G_rGCy'C Cuts at: 1391 3013 3366 3567 3926 4201 4419 4583 1391 Size: 1622 353 201 359 275 218 164 4072 Fragments arranged by size: 4072 1622 359 353 275 218 201 164 BbeI G_GCGC'C Cuts at: 3619 3619 Size: 7264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2064 3151 928 Size: 971 165 1087 5041 Fragments arranged by size: 5041 1087 971 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2442 2476 2804 2838 4399 4433 5455 Size: 34 505 34 328 34 1561 34 1022 Cuts at: 5455 5489 1903 Size: 34 3678 Fragments arranged by size: 3678 1561 1022 505 328 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5156 6366 5156 Size: 1210 6054 BclI T'GATC_A Cuts at: 1548 1548 Size: 7264 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2388 5875 136 Size: 107 122 71 631 605 716 3487 1525 Fragments arranged by size: 3487 1525 716 631 605 122 107 71 BglII A'GATC_T Cuts at: 7259 7259 Size: 7264 BlpI GC'TnA_GC Cuts at: 2195 4681 2195 Size: 2486 4778 Bme1580I G_kGCm'C Cuts at: 1790 3013 3533 3926 4201 4412 4446 4480 5325 Size: 1223 520 393 275 211 34 34 845 Cuts at: 5325 6481 1790 Size: 1156 2573 Fragments arranged by size: 2573 1223 1156 845 520 393 275 211 34 34 BmgBI CAC'GTC Cuts at: 1318 3886 4456 1318 Size: 2568 570 4126 Fragments arranged by size: 4126 2568 570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2252 2852 3042 Size: 416 53 83 399 24 835 600 190 Cuts at: 3042 4995 5953 442 Size: 1953 958 1753 Fragments arranged by size: 1953 1753 958 835 600 416 399 190 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2323 2942 2988 3536 3589 4166 4525 Size: 899 185 619 46 548 53 577 359 Cuts at: 4525 5924 1239 Size: 1399 2579 Fragments arranged by size: 2579 1399 899 619 577 548 359 185 53 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 3413 5389 6254 6552 3185 Size: 228 1976 865 298 3897 Fragments arranged by size: 3897 1976 865 298 228 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2824 5927 882 Size: 617 1325 3103 2219 Fragments arranged by size: 3103 2219 1325 617 BsaAI yAC'GTr Cuts at: 493 1204 3877 5055 493 Size: 711 2673 1178 2702 Fragments arranged by size: 2702 2673 1178 711 BsaBI GATnn'nnATC Cuts at: 3243 3423 7258 3243 Size: 180 3835 3249 Fragments arranged by size: 3835 3249 180 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2543 3606 3616 Size: 53 83 186 936 510 500 1063 10 Cuts at: 3616 5071 5453 6088 275 Size: 1455 382 635 1451 Fragments arranged by size: 1455 1451 1063 936 635 510 500 382 186 83 53 10 BsaWI w'CCGG_w Cuts at: 5697 6095 6369 6516 6927 5697 Size: 398 274 147 411 6034 Fragments arranged by size: 6034 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2321 2351 1178 Size: 30 165 30 678 30 210 30 6091 Fragments arranged by size: 6091 678 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 3842 1186 Size: 865 1791 4608 Fragments arranged by size: 4608 1791 865 BseYI C'CCAG_C Cuts at: 2305 2489 2957 4492 6467 6963 2305 Size: 184 468 1535 1975 496 2606 Fragments arranged by size: 2606 1975 1535 496 468 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3117 3421 1163 Size: 573 1381 304 5006 Fragments arranged by size: 5006 1381 573 304 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3546 4076 4714 5475 6503 1540 Size: 275 186 1545 530 638 761 1028 2301 Fragments arranged by size: 2301 1545 1028 761 638 530 275 186 BsiHKAI G_wGCw'C Cuts at: 4412 4419 4446 5325 5410 6481 4412 Size: 7 27 879 85 1071 5195 Fragments arranged by size: 5195 1071 879 85 27 7 BsmI GAATG_Cn' Cuts at: 4775 4868 4775 Size: 93 7171 BsmBI CGTCTCn'nnnn_ Cuts at: 2437 2896 2437 Size: 459 6805 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3013 3366 3533 3567 3926 4201 4412 Size: 399 1223 353 167 34 359 275 211 Cuts at: 4412 4419 4446 4480 4583 5325 5410 6481 1391 Size: 7 27 34 103 742 85 1071 2174 Fragments arranged by size: 2174 1223 1071 742 399 359 353 275 211 167 103 85 34 34 27 7 BspEI T'CCGG_A Cuts at: 6927 6927 Size: 7264 BspHI T'CATG_A Cuts at: 4380 5153 6883 4380 Size: 773 1730 4761 Fragments arranged by size: 4761 1730 773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 4044 4619 844 Size: 1761 1439 575 3489 Fragments arranged by size: 3489 1761 1439 575 BsrBI CCG'CTC Cuts at: 1394 1823 5151 1394 Size: 429 3328 3507 Fragments arranged by size: 3507 3328 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3834 5759 5933 65 Size: 1183 2586 1925 174 1396 Fragments arranged by size: 2586 1925 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2382 3492 4011 4071 5908 6095 Size: 110 235 337 1110 519 60 1837 187 Cuts at: 6095 1700 Size: 2869 Fragments arranged by size: 2869 1837 1110 519 337 235 187 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7264 BssSI C'ACGA_G Cuts at: 2687 3481 3857 5324 6336 2687 Size: 794 376 1467 1012 3615 Fragments arranged by size: 3615 1467 1012 794 376 BstAPI GCAn_nnn'nTGC Cuts at: 794 3444 794 Size: 2650 4614 BstBI TT'CG_AA Cuts at: 4023 6068 4023 Size: 2045 5219 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7264 BstXI CCAn_nnnn'nTGG Cuts at: 2795 2795 Size: 7264 BstYI r'GATC_y Cuts at: 1972 4425 4594 4961 5346 5363 6815 6901 6913 Size: 2453 169 367 385 17 1452 86 12 Cuts at: 6913 7259 1972 Size: 346 1977 Fragments arranged by size: 2453 1977 1452 385 367 346 169 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 2474 2727 3013 4471 513 Size: 552 1350 59 253 286 1458 3306 Fragments arranged by size: 3306 1458 1350 552 286 253 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2235 2814 3739 487 Size: 683 56 1009 579 925 4012 Fragments arranged by size: 4012 1009 925 683 579 56 BtsI GCAGTG_nn' Cuts at: 4780 5574 5594 4780 Size: 794 20 6450 Fragments arranged by size: 6450 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7264 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7229 DraI TTT'AAA Cuts at: 4568 4568 Size: 7264 DraIII CAC_nnn'GTG Cuts at: 3444 3444 Size: 7264 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2258 6271 809 Size: 507 942 4013 1802 Fragments arranged by size: 4013 1802 942 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2477 2871 3543 Size: 54 1425 50 275 18 647 394 672 Cuts at: 3543 4073 4711 5600 7229 8 Size: 530 638 889 1629 43 Fragments arranged by size: 1629 1425 889 672 647 638 530 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3543 4073 4711 1537 Size: 275 1731 530 638 4090 Fragments arranged by size: 4090 1731 638 530 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2279 4022 4535 5194 1353 Size: 926 1743 513 659 3423 Fragments arranged by size: 3423 1743 926 659 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3960 5851 6225 6371 387 Size: 1064 138 2371 1891 374 146 1280 Fragments arranged by size: 2371 1891 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4417 4417 Size: 7264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2323 2942 2988 3011 3252 3536 Size: 393 506 185 619 46 23 241 284 Cuts at: 3536 3542 3589 3837 4166 4525 4562 5327 5924 Size: 6 47 248 329 359 37 765 597 Cuts at: 5924 6711 6979 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 765 619 597 506 393 359 329 284 268 248 241 185 47 46 37 23 6 EcoNI CCTnn'n_nnAGG Cuts at: 2579 3998 2579 Size: 1419 5845 EcoO109I rG'GnC_Cy Cuts at: 2079 2436 2683 3043 3922 4197 4500 2079 Size: 357 247 360 879 275 303 4843 Fragments arranged by size: 4843 879 360 357 303 275 247 EcoRI G'AATT_C Cuts at: 4573 4951 4573 Size: 378 6886 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4567 4567 Size: 7264 FspI TGC'GCA Cuts at: 5770 5770 Size: 7264 HaeII r_GCGC'y Cuts at: 1678 3199 3598 3619 6411 1678 Size: 1521 399 21 2792 2531 Fragments arranged by size: 2792 2531 1521 399 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 5905 5937 5979 Size: 32 135 32 509 32 3792 32 42 Cuts at: 5979 6011 1373 Size: 32 2626 Fragments arranged by size: 3792 2626 509 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2074 4608 4854 669 Size: 988 281 136 2534 246 3079 Fragments arranged by size: 3079 2534 988 281 246 136 HindIII A'AGCT_T Cuts at: 748 3554 748 Size: 2806 4458 HpaI GTT'AAC Cuts at: 4854 4854 Size: 7264 KasI G'GCGC_C Cuts at: 3615 3615 Size: 7264 KpnI G_GTAC'C Cuts at: 4589 4589 Size: 7264 MfeI C'AATT_G Cuts at: 4863 4863 Size: 7264 MluI A'CGCG_T Cuts at: 7140 7140 Size: 7264 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2982 3823 4485 4546 6378 6562 1305 Size: 819 858 841 662 61 1832 184 2007 Fragments arranged by size: 2007 1832 858 841 819 662 184 61 MscI TGG'CCA Cuts at: 10 64 1489 1832 2873 10 Size: 54 1425 343 1041 4401 Fragments arranged by size: 4401 1425 1041 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2028 2732 4454 5222 5581 5740 518 Size: 648 862 704 1722 768 359 159 2042 Fragments arranged by size: 2042 1722 862 768 704 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2309 2989 3155 3542 4680 5357 6505 6750 Size: 703 680 166 387 1138 677 1148 245 Cuts at: 6750 1606 Size: 2120 Fragments arranged by size: 2120 1148 1138 703 680 677 387 245 166 NaeI GCC'GGC Cuts at: 2047 2384 4013 2047 Size: 337 1629 5298 Fragments arranged by size: 5298 1629 337 NarI GG'CG_CC Cuts at: 3616 3616 Size: 7264 NcoI C'CATG_G Cuts at: 513 1065 2474 2727 513 Size: 552 1409 253 5050 Fragments arranged by size: 5050 1409 552 253 NdeI CA'TA_TG Cuts at: 387 387 Size: 7264 NgoMIV G'CCGG_C Cuts at: 2045 2382 4011 2045 Size: 337 1629 5298 Fragments arranged by size: 5298 1629 337 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7264 NotI GC'GGCC_GC Cuts at: 4711 4711 Size: 7264 NruI TCG'CGA Cuts at: 2164 3758 2164 Size: 1594 5670 NspI r_CATG'y Cuts at: 1132 1229 2779 3378 3409 3583 4560 4622 6167 Size: 97 1550 599 31 174 977 62 1545 Cuts at: 6167 1132 Size: 2229 Fragments arranged by size: 2229 1550 1545 977 599 174 97 62 31 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7264 PciI A'CATG_T Cuts at: 3405 3579 6163 3405 Size: 174 2584 4506 Fragments arranged by size: 4506 2584 174 PflMI CCAn_nnn'nTGG Cuts at: 1108 2920 1108 Size: 1812 5452 PfoI T'CCnGG_A Cuts at: 2115 2277 4143 4502 2115 Size: 162 1866 359 4877 Fragments arranged by size: 4877 1866 359 162 PmeI GTTT'AAAC Cuts at: 4568 4568 Size: 7264 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5333 5365 6846 6878 5333 Size: 32 1481 32 5719 Fragments arranged by size: 5719 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2436 2683 4500 2436 Size: 247 1817 5200 Fragments arranged by size: 5200 1817 247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7264 PsiI TTA'TAA Cuts at: 4834 4834 Size: 7264 PspOMI G'GGCC_C Cuts at: 3009 3922 4197 3009 Size: 913 275 6076 Fragments arranged by size: 6076 913 275 PspXI vC'TCGA_Gb Cuts at: 4265 4265 Size: 7264 PstI C_TGCA'G Cuts at: 830 2087 2827 3645 4275 4616 830 Size: 1257 740 818 630 341 3478 Fragments arranged by size: 3478 1257 818 740 630 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7264 PvuII CAG'CTG Cuts at: 2309 2989 3155 2309 Size: 680 166 6418 Fragments arranged by size: 6418 680 166 SacI G_AGCT'C Cuts at: 4419 4419 Size: 7264 SalI G'TCGA_C Cuts at: 1936 4606 1936 Size: 2670 4594 SapI GCTCTTCn'nnn_ Cuts at: 2279 4022 2279 Size: 1743 5521 SbfI CC_TGCA'GG Cuts at: 3645 4616 3645 Size: 971 6293 ScaI AGT'ACT Cuts at: 5512 7224 5512 Size: 1712 5552 SexAI A'CCwGG_T Cuts at: 2432 2432 Size: 7264 SfcI C'TryA_G Cuts at: 826 1045 2083 2823 3641 4271 4612 5747 6428 Size: 219 1038 740 818 630 341 1135 681 Cuts at: 6428 6619 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1038 818 740 681 630 341 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2388 2388 Size: 7264 SfoI GGC'GCC Cuts at: 3617 3617 Size: 7264 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7264 SgrAI Cr'CCGG_yG Cuts at: 1700 2045 1700 Size: 345 6919 SmaI CCC'GGG Cuts at: 1872 4225 4591 1872 Size: 2353 366 4545 Fragments arranged by size: 4545 2353 366 SmlI C'TyrA_G Cuts at: 820 1017 1943 3200 3392 4265 5368 6269 6531 Size: 197 926 1257 192 873 1103 901 262 Cuts at: 6531 820 Size: 1553 Fragments arranged by size: 1553 1257 1103 926 901 873 262 197 192 SnaBI TAC'GTA Cuts at: 493 5055 493 Size: 4562 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7264 SphI G_CATG'C Cuts at: 1132 2779 4622 1132 Size: 1647 1843 3774 Fragments arranged by size: 3774 1843 1647 SspI AAT'ATT Cuts at: 5 52 3910 5188 5 Size: 47 3858 1278 2081 Fragments arranged by size: 3858 2081 1278 47 StuI AGG'CCT Cuts at: 1517 2359 3647 3993 1517 Size: 842 1288 346 4788 Fragments arranged by size: 4788 1288 842 346 StyI C'CwwG_G Cuts at: 513 1065 2474 2727 4056 4703 513 Size: 552 1409 253 1329 647 3074 Fragments arranged by size: 3074 1409 1329 647 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3205 3246 5291 5308 5461 5646 6091 Size: 1255 1625 41 2045 17 153 185 445 Cuts at: 6091 325 Size: 1498 Fragments arranged by size: 2045 1625 1498 1255 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4315 5510 7222 Size: 274 80 33 51 1443 2338 1195 1712 Cuts at: 7222 96 Size: 138 Fragments arranged by size: 2338 1712 1443 1195 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 2675 2707 7224 7256 2345 Size: 32 298 32 4517 32 2353 Fragments arranged by size: 4517 2353 298 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7264 XbaI T'CTAG_A Cuts at: 4600 4600 Size: 7264 XhoI C'TCGA_G Cuts at: 1943 4265 1943 Size: 2322 4942 XmaI C'CCGG_G Cuts at: 1870 4223 4589 1870 Size: 2353 366 4545 Fragments arranged by size: 4545 2353 366 XmnI GAAnn'nnTTC Cuts at: 5393 5393 Size: 7264 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5072 276 Size: 53 83 186 936 3538 2468 Fragments arranged by size: 3538 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NsiI PacI PmlI PsrI RsrII SacII SanDI SrfI SwaI XcmI