Restriction map for
pFN21AB8011
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstYI BstZ17I Bsu36I Cfr10I
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 344 AAATTC [GA]^AATT[CT]
AflIII 447 ACATGT A^C[GA][CT]GT
Alw44I 443 GTGCAC G^TGCAC
AlwI 1212 GATCC GGATC(4/5)
AlwNI 749 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 6 AGCGCT AGC^GCT
ApaLI 443 GTGCAC G^TGCAC
ApoI 344 AAATTC [GA]^AATT[CT]
AspHI 443 GTGCAC G[AT]GC[AT]^C
BbsI 120 GAAGAC GAAGAC(2/6)
BcgI 953 CGAACGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1232 GCCAGTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 120 GAAGAC GAAGAC(2/6)
BsaBI 283 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 443 GTGCAC G[AT]GC[AT]^C
BsmBI 1174 GAGACG CGTCTC(1/5)
BsmI 586 GCATTC GAATGC(1/-1)
Bsp1407I 1010 TGTACA T^GTACA
BspDI 788 ATCGAT AT^CGAT
BspLU11I 447 ACATGT A^CATGT
BsrBI 744 CCGCTC CCGCTC(-3/-3)
BsrGI 1010 TGTACA T^GTACA
BstAPI 634 GCAACTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 412 TTCGAA TT^CGAA
ClaI 788 ATCGAT AT^CGAT
Eco47III 6 AGCGCT AGC^GCT
EcoNI 1028 CCTTGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 226 GTTGAC GT[CT]^[GA]AC
HindII 226 GTTGAC GT[CT]^[GA]AC
MaeII 949 ACGT A^CGT
MamI 283 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 803 TTAA T^TAA
NspV 412 TTCGAA TT^CGAA
PflMI 139 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 826 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 462 CTGCAG CTGCA^G
PvuII 999 CAGCTG CAG^CTG
SbfI 461 CCTGCAGG CCTGCA^GG
SfcI 462 CTGCAG C^T[GA][CT]AG
SfuI 412 TTCGAA TT^CGAA
SphI 1145 GCATGC GCATG^C
Sse8387I 461 CCTGCAGG CCTGCA^GG
SspBI 1010 TGTACA T^GTACA
StuI 1036 AGGCCT AGG^CCT
TfiI 693 GAATC G^A[AT]TC
Tru9I 803 TTAA T^TAA
Van91I 139 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 18 CGCG CG^CG
940 CGCG
BlpI 746 GCTCAGC GC^T[ACGT]AGC
1197 GCTCAGC
BmyI 443 GTGCAC G[AGT]GC[ACT]^C
703 GGGCAC
Bpu1102I 746 GCTCAGC GC^T[ACGT]AGC
1197 GCTCAGC
BsaI 676 GAGACC GGTCTC(1/5)
771 GGTCTC
BsgI 919 CTGCAC GTGCAG(16/14)
568 GTGCAG
BsmFI 783 GTCCC GGGAC(10/14)
1093 GTCCC
Bsp1286I 443 GTGCAC G[AGT]GC[ACT]^C
703 GGGCAC
BstUI 18 CGCG CG^CG
940 CGCG
CelII 746 GCTCAGC GC^T[ACGT]AGC
1197 GCTCAGC
DpnII 453 GATC ^GATC
1212 GATC
DsaI 338 CCATGG C^C[GA][CT]GG
438 CCATGG
EarI 608 CTCTTC CTCTTC(1/4)
1171 GAAGAG
FauI 19 GCGGG CCCGC(4/6)
941 GCGGG
HaeII 6 AGCGCT [GA]GCGC^[CT]
1194 GGCGCT
Ksp632I 608 CTCTTC CTCTTC(1/4)
1171 GAAGAG
MboI 453 GATC ^GATC
1212 GATC
MspA1I 999 CAGCTG C[AC]G^C[GT]G
1140 CCGCTG
MvnI 18 CGCG CG^CG
940 CGCG
NcoI 338 CCATGG C^CATGG
438 CCATGG
NdeII 453 GATC ^GATC
1212 GATC
NlaIV 258 GGACCC GG[ACGT]^[ACGT]CC
1184 GGACCC
NspI 447 ACATGT [GA]CATG^[CT]
1145 GCATGC
Sau3AI 453 GATC ^GATC
1212 GATC
SfaNI 867 GATGC GCATC(5/9)
963 GCATC
Tsp509I 345 AATT ^AATT
397 AATT
XcmI 662 CCAAAGATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1215 CCAAGAACGGCTTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MslI MspI MvaI MwoI
NciI NlaIII PleI RsaI Sau96I
ScrFI StyI TaqI TseI Tsp45I
TspRI TthHB8I