(Circular) (Six-base) MAPSORT of: pFN21AB8011.seq Check: 3984 from: 1 to: 5947 pFN21AB8011 5947 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2607 2818 4010 5394 5662 1632 Size: 975 211 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 975 268 211 AfeI AGC'GCT Cuts at: 2013 2013 Size: 5947 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2452 4846 5823 1203 Size: 1249 2394 977 1327 Fragments arranged by size: 2394 1327 1249 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 3080 1166 Size: 1914 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2598 1184 Size: 1414 4533 AlwNI CAG_nnn'CTG Cuts at: 2759 3690 5262 2759 Size: 931 1572 3444 Fragments arranged by size: 3444 1572 931 ApaLI G'TGCA_C Cuts at: 2448 4004 5160 2448 Size: 1556 1156 3235 Fragments arranged by size: 3235 1556 1156 ApoI r'AATT_y Cuts at: 1493 2349 3256 3482 3634 5743 1493 Size: 856 907 226 152 2109 1697 Fragments arranged by size: 2109 1697 907 856 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 4723 618 Size: 325 217 627 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3266 1391 Size: 1875 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2132 928 Size: 971 233 4743 Fragments arranged by size: 4743 971 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2947 2981 4138 4172 1903 Size: 34 1010 34 1157 34 3678 Fragments arranged by size: 3678 1157 1010 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3243 4558 136 Size: 107 122 71 631 605 1571 1315 1525 Fragments arranged by size: 1571 1525 1315 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 2752 3203 3364 2752 Size: 451 161 5335 Fragments arranged by size: 5335 451 161 Bme1580I G_kGCm'C Cuts at: 1790 2452 2712 4008 5164 1790 Size: 662 260 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 662 260 BmgBI CAC'GTC Cuts at: 1318 2955 1318 Size: 1637 4310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2415 2677 2699 2717 3128 4607 1239 Size: 1100 76 262 22 18 411 1479 2579 Fragments arranged by size: 2579 1479 1100 411 262 76 22 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2675 2782 4610 882 Size: 617 1176 107 1828 2219 Fragments arranged by size: 2219 1828 1176 617 107 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 2292 5941 2292 Size: 3649 2298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 2135 2720 2770 1186 Size: 901 48 585 50 4363 Fragments arranged by size: 4363 901 585 50 48 BseYI C'CCAG_C Cuts at: 2067 2094 5150 5646 2067 Size: 27 3056 496 2368 Fragments arranged by size: 3056 2368 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 2909 1163 Size: 573 858 315 4201 Fragments arranged by size: 4201 858 573 315 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2452 4008 4093 5164 2452 Size: 1556 85 1071 3235 Fragments arranged by size: 3235 1556 1071 85 BsmI GAATG_Cn' Cuts at: 2591 3458 3551 2591 Size: 867 93 4987 Fragments arranged by size: 4987 867 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3173 3173 Size: 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2452 2712 3266 4008 4093 5164 1391 Size: 399 662 260 554 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 662 554 399 260 85 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2751 3834 1394 Size: 429 928 1083 3507 Fragments arranged by size: 3507 1083 928 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 4778 1700 Size: 110 2781 187 2869 Fragments arranged by size: 2869 2781 187 110 BsrGI T'GTAC_A Cuts at: 96 3015 96 Size: 2919 3028 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 2645 794 Size: 1851 4096 BstBI TT'CG_AA Cuts at: 2418 4751 2418 Size: 2333 3614 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2190 2260 2344 2190 Size: 70 84 5793 Fragments arranged by size: 5793 84 70 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2343 2443 513 Size: 552 939 339 100 4017 Fragments arranged by size: 4017 939 552 339 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2885 2984 487 Size: 683 56 1659 99 3450 Fragments arranged by size: 3450 1659 683 99 56 BtsI GCAGTG_nn' Cuts at: 2337 2502 2970 3020 3463 4257 4277 2337 Size: 165 468 50 443 794 20 4007 Fragments arranged by size: 4007 794 468 443 165 50 20 ClaI AT'CG_AT Cuts at: 1554 2794 1554 Size: 1240 4707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912 Size: 54 1425 50 275 18 1564 889 1629 Cuts at: 5912 8 Size: 43 Fragments arranged by size: 1629 1564 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 3171 3877 1353 Size: 1266 552 706 3423 Fragments arranged by size: 3423 1266 706 552 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2415 2607 2677 2699 2717 2818 Size: 393 707 76 192 70 22 18 101 Cuts at: 2818 3128 4010 4607 5394 5662 1239 Size: 310 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 707 597 393 310 268 192 101 76 70 22 18 EcoNI CCTnn'n_nnAGG Cuts at: 3037 3037 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3245 3245 Size: 5947 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 2015 3203 5094 1678 Size: 337 1188 1891 2531 Fragments arranged by size: 2531 1891 1188 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4588 4620 4662 4694 1373 Size: 32 135 32 3016 32 42 32 2626 Fragments arranged by size: 3016 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2233 3291 3537 669 Size: 988 281 295 1058 246 3079 Fragments arranged by size: 3079 1058 988 295 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5947 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2321 2873 2940 3015 5061 5245 1305 Size: 1016 552 67 75 2046 184 2007 Fragments arranged by size: 2046 2007 1016 552 184 75 67 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2188 2342 3080 3905 4264 4423 518 Size: 648 1022 154 738 825 359 159 2042 Fragments arranged by size: 2042 1022 825 738 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 3006 3147 3363 4040 5188 5433 1606 Size: 1400 141 216 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 245 216 141 NcoI C'CATG_G Cuts at: 513 1065 2004 2343 2443 513 Size: 552 939 339 100 4017 Fragments arranged by size: 4017 939 552 339 100 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2456 3154 3305 4850 1132 Size: 97 1227 698 151 1545 2229 Fragments arranged by size: 2229 1545 1227 698 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 2452 4846 2452 Size: 2394 3553 PflMI CCAn_nnn'nTGG Cuts at: 1108 2150 1108 Size: 1042 4905 PfoI T'CCnGG_A Cuts at: 2733 2971 2733 Size: 238 5709 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2835 1613 Size: 1222 4725 PsiI TTA'TAA Cuts at: 2883 3517 2883 Size: 634 5313 PstI C_TGCA'G Cuts at: 830 2471 3299 830 Size: 1641 828 3478 Fragments arranged by size: 3478 1641 828 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 PvuII CAG'CTG Cuts at: 3006 3006 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SbfI CC_TGCA'GG Cuts at: 2471 3299 2471 Size: 828 5119 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2467 3295 4430 5111 5302 826 Size: 219 1422 828 1135 681 191 1471 Fragments arranged by size: 1471 1422 1135 828 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3154 3305 1132 Size: 2022 151 3774 Fragments arranged by size: 3774 2022 151 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 3043 1517 Size: 1526 4421 StyI C'CwwG_G Cuts at: 513 1065 2004 2085 2343 2443 2529 2817 3033 Size: 552 939 81 258 100 86 288 216 Cuts at: 3033 3386 513 Size: 353 3074 Fragments arranged by size: 3074 939 552 353 288 258 216 100 86 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3001 3974 3991 4144 4329 4774 325 Size: 1255 1421 973 17 153 185 445 1498 Fragments arranged by size: 1498 1421 1255 973 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2985 3015 3084 Size: 274 80 33 51 1443 1008 30 69 Cuts at: 3084 3126 4193 5905 96 Size: 42 1067 1712 138 Fragments arranged by size: 1712 1443 1067 1008 274 138 80 69 51 42 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2729 2761 5907 5939 2729 Size: 32 3146 32 2737 Fragments arranged by size: 3146 2737 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2674 3227 2674 Size: 553 5394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI