Restriction map for pFN21AE0503
 (Linear) (Six-base) MAPSORT of: KIEE0503.seq  Check: 8723  from: 1  to: 660



KIEE0503.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     660 
   Size:        328     332
 
AfeI AGC'GCT

Cuts at:      0       7     124     660 
   Size:          7     117     536

  Fragments arranged by size: 

                 536     117       7
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     635     660 
   Size:        635      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0      72      90     246     285     516     660 
   Size:         72      18     156      39     231     144

  Fragments arranged by size: 

                 231     156     144      72      39      18
 
BamHI G'GATC_C

Cuts at:      0     386     660 
   Size:        386     274
 
BanI G'GyrC_C

Cuts at:      0      16      40     172     660 
   Size:         16      24     132     488

  Fragments arranged by size: 

                 488     132      24      16
 
BbeI G_GCGC'C

Cuts at:      0      20     660 
   Size:         20     640
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     162     196     660 
   Size:        162      34     464

  Fragments arranged by size: 

                 464     162      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     313     660 
   Size:        313     347
 
BlpI GC'TnA_GC

Cuts at:      0     142     353     660 
   Size:        142     211     307

  Fragments arranged by size: 

                 307     211     142
 
Bme1580I G_kGCm'C

Cuts at:      0      45     660 
   Size:         45     615
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     621     660 
   Size:        621      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     448     487     660 
   Size:        223     225      39     173

  Fragments arranged by size: 

                 225     223     173      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     274     660 
   Size:        274     386
 
BsaAI yAC'GTr

Cuts at:      0     495     660 
   Size:        495     165
 
BsaHI Gr'CG_yC

Cuts at:      0      17     660 
   Size:         17     643
 
BsaWI w'CCGG_w

Cuts at:      0     383     437     548     660 
   Size:        383      54     111     112

  Fragments arranged by size: 

                 383     112     111      54
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     409     660 
   Size:         40     369     251

  Fragments arranged by size: 

                 369     251      40
 
BseYI C'CCAG_C

Cuts at:      0     106     308     572     660 
   Size:        106     202     264      88

  Fragments arranged by size: 

                 264     202     106      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     660 
   Size:        211     449
 
BsiHKAI G_wGCw'C

Cuts at:      0     275     383     463     660 
   Size:        275     108      80     197

  Fragments arranged by size: 

                 275     197     108      80
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     275     383     463     660 
   Size:         45     230     108      80     197

  Fragments arranged by size: 

                 230     197     108      80      45
 
BspEI T'CCGG_A

Cuts at:      0     548     660 
   Size:        548     112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     341     542     660 
   Size:        341     201     118

  Fragments arranged by size: 

                 341     201     118
 
BsrFI r'CCGG_y

Cuts at:      0     320     660 
   Size:        320     340
 
BsrGI T'GTAC_A

Cuts at:      0     233     660 
   Size:        233     427
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     270     285     660 
   Size:        270      15     375

  Fragments arranged by size: 

                 375     270      15
 
BstBI TT'CG_AA

Cuts at:      0     182     660 
   Size:        182     478
 
BstYI r'GATC_y

Cuts at:      0     206     386     660 
   Size:        206     180     274

  Fragments arranged by size: 

                 274     206     180
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     421     660 
   Size:        421     239
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      49     660 
   Size:         49     611
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     328     448     487     660 
   Size:        223     105     120      39     173

  Fragments arranged by size: 

                 223     173     120     105      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      94     660 
   Size:         94     566
 
EcoO109I rG'GnC_Cy

Cuts at:      0     604     660 
   Size:        604      56
 
HaeII r_GCGC'y

Cuts at:      0       9      20     126     163     246     286     660 
   Size:          9      11     106      37      83      40     374

  Fragments arranged by size: 

                 374     106      83      40      37      11       9
 
KasI G'GCGC_C

Cuts at:      0      16     660 
   Size:         16     644
 
MspA1I CmG'CkG

Cuts at:      0      69     402     660 
   Size:         69     333     258

  Fragments arranged by size: 

                 333     258      69
 
NaeI GCC'GGC

Cuts at:      0     322     660 
   Size:        322     338
 
NarI GG'CG_CC

Cuts at:      0      17     660 
   Size:         17     643
 
NgoMIV G'CCGG_C

Cuts at:      0     320     660 
   Size:        320     340
 
PasI CC'CwG_GG

Cuts at:      0     591     660 
   Size:        591      69
 
PfoI T'CCnGG_A

Cuts at:      0     425     660 
   Size:        425     235
 
PpuMI rG'GwC_Cy

Cuts at:      0     604     660 
   Size:        604      56
 
PstI C_TGCA'G

Cuts at:      0     527     560     660 
   Size:        527      33     100

  Fragments arranged by size: 

                 527     100      33
 
PvuII CAG'CTG

Cuts at:      0      69     660 
   Size:         69     591
 
SfcI C'TryA_G

Cuts at:      0     253     523     556     660 
   Size:        253     270      33     104

  Fragments arranged by size: 

                 270     253     104      33
 
SfoI GGC'GCC

Cuts at:      0      18     660 
   Size:         18     642
 
StuI AGG'CCT

Cuts at:      0     316     660 
   Size:        316     344
 
StyI C'CwwG_G

Cuts at:      0      20     660 
   Size:         20     640
 
TatI w'GTAC_w

Cuts at:      0     233     660 
   Size:        233     427
 

 Enzymes that do cut:

     AcuI     AfeI     AlfI    AlwNI    BamHI     BanI     BbeI     BcgI
     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrFI    BsrGI   BstAPI    BstBI    BstYI    BtgZI     EciI  Eco57MI
    EcoNI EcoO109I    HaeII     KasI   MspA1I     NaeI     NarI   NgoMIV
     PasI     PfoI    PpuMI     PstI    PvuII     SfcI     SfoI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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