(Circular) (Six-base) MAPSORT of: pFN21AE0524.seq Check: 8711 from: 1 to: 5422 pFN21AE0524 5422 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2029 2029 Size: 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2568 3232 278 Size: 53 83 186 936 1032 664 2468 Fragments arranged by size: 2468 1032 936 664 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3485 4869 5137 1632 Size: 977 876 1384 268 1917 Fragments arranged by size: 1917 1384 977 876 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 2394 4321 5298 1203 Size: 1191 1927 977 1327 Fragments arranged by size: 1927 1327 1191 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaI G_GGCC'C Cuts at: 2202 2202 Size: 5422 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2415 2747 1870 Size: 73 472 332 4545 Fragments arranged by size: 4545 472 332 73 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2455 2713 2743 4198 Size: 325 217 627 254 414 258 30 1455 Cuts at: 4198 618 Size: 1842 Fragments arranged by size: 1842 1455 627 414 325 258 254 217 30 BanII G_rGCy'C Cuts at: 1391 2109 2202 2270 2709 2741 1391 Size: 718 93 68 439 32 4072 Fragments arranged by size: 4072 718 439 93 68 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2290 2560 928 Size: 971 391 270 3790 Fragments arranged by size: 3790 971 391 270 BbvCI CC'TCA_GC Cuts at: 2111 2111 Size: 5422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 2685 4033 Size: 107 122 71 631 605 706 307 1348 Cuts at: 4033 136 Size: 1525 Fragments arranged by size: 1525 1348 706 631 605 307 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2839 2839 Size: 5422 Bme1580I G_kGCm'C Cuts at: 1790 2202 3483 4639 1790 Size: 412 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 4111 442 Size: 416 53 83 399 24 1736 958 1753 Fragments arranged by size: 1753 1736 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2188 2579 2604 4082 1239 Size: 884 65 391 25 1478 2579 Fragments arranged by size: 2579 1478 884 391 65 25 Bpu10I CC'TnA_GC Cuts at: 2111 2111 Size: 5422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 3213 493 Size: 711 1191 818 2702 Fragments arranged by size: 2702 1191 818 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 2565 3229 3611 Size: 53 83 186 936 786 246 664 382 Cuts at: 3611 4246 275 Size: 635 1451 Fragments arranged by size: 1451 936 786 664 635 382 246 186 83 53 BsaWI w'CCGG_w Cuts at: 2151 3855 4253 4527 4674 5085 2151 Size: 1704 398 274 147 411 2488 Fragments arranged by size: 2488 1704 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 4509 BseYI C'CCAG_C Cuts at: 2335 2497 2524 2641 4625 5121 2335 Size: 162 27 117 1984 496 2636 Fragments arranged by size: 2636 1984 496 162 117 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2117 1163 Size: 573 381 4468 Fragments arranged by size: 4468 573 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2028 2281 2289 2872 3633 4661 Size: 275 186 27 253 8 583 761 1028 Cuts at: 4661 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 583 275 253 186 27 8 BsiHKAI G_wGCw'C Cuts at: 2109 2709 3483 3568 4639 2109 Size: 600 774 85 1071 2892 Fragments arranged by size: 2892 1071 774 600 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2202 2270 2709 2741 3483 3568 Size: 399 319 93 68 439 32 742 85 Cuts at: 3568 4639 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 319 93 85 68 32 BspEI T'CCGG_A Cuts at: 2151 5085 2151 Size: 2934 2488 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2029 2529 2777 844 Size: 1185 500 248 3489 Fragments arranged by size: 3489 1185 500 248 BsrBI CCG'CTC Cuts at: 1394 1823 3309 1394 Size: 429 1486 3507 Fragments arranged by size: 3507 1486 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4066 4253 1700 Size: 110 234 2022 187 2869 Fragments arranged by size: 2869 2022 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 2513 3482 4494 2513 Size: 969 1012 3441 Fragments arranged by size: 3441 1012 969 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2515 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417 Size: 780 367 385 17 1452 86 12 346 Cuts at: 5417 1972 Size: 1977 Fragments arranged by size: 1977 1452 780 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 2275 513 Size: 552 939 87 184 3660 Fragments arranged by size: 3660 939 552 184 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2278 2286 Size: 54 1425 50 275 18 195 253 8 Cuts at: 2286 2379 2869 3758 5387 8 Size: 93 490 889 1629 43 Fragments arranged by size: 1629 1425 889 490 275 253 195 93 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2025 2278 2286 2869 1537 Size: 275 213 253 8 583 4090 Fragments arranged by size: 4090 583 275 253 213 8 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 2237 2256 2315 3352 1353 Size: 842 42 19 59 1037 3423 Fragments arranged by size: 3423 1037 842 59 42 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4009 4383 4529 387 Size: 1064 138 2420 374 146 1280 Fragments arranged by size: 2420 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2107 2707 2107 Size: 600 4822 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2188 2579 2604 2609 3485 4082 Size: 393 491 65 391 25 5 876 597 Cuts at: 4082 4869 5137 1239 Size: 787 268 1524 Fragments arranged by size: 1524 876 787 597 491 393 391 268 65 25 5 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 5422 EcoO109I rG'GnC_Cy Cuts at: 2199 2371 2638 2199 Size: 172 267 4983 Fragments arranged by size: 4983 267 172 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2477 4569 1678 Size: 799 2092 2531 Fragments arranged by size: 2531 2092 799 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2428 2766 3012 669 Size: 988 281 490 338 246 3079 Fragments arranged by size: 3079 988 490 338 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 10 Size: 54 1425 343 549 3051 Fragments arranged by size: 3051 1425 549 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2387 2513 3380 3739 3898 518 Size: 648 1215 6 126 867 359 159 2042 Fragments arranged by size: 2042 1215 867 648 359 159 126 6 MspA1I CmG'CkG Cuts at: 1606 2093 2101 2277 2645 2838 3515 4663 4908 Size: 487 8 176 368 193 677 1148 245 Cuts at: 4908 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 487 368 245 193 176 8 NaeI GCC'GGC Cuts at: 2046 2046 Size: 5422 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NgoMIV G'CCGG_C Cuts at: 2044 2044 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2278 2869 2278 Size: 591 4831 NspI r_CATG'y Cuts at: 1132 1229 2230 2257 2780 4325 1132 Size: 97 1001 27 523 1545 2229 Fragments arranged by size: 2229 1545 1001 523 97 27 PasI CC'CwG_GG Cuts at: 1348 2203 2690 1348 Size: 855 487 4080 Fragments arranged by size: 4080 855 487 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4520 PfoI T'CCnGG_A Cuts at: 2556 2556 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2198 2198 Size: 5422 PstI C_TGCA'G Cuts at: 830 2040 2604 2774 830 Size: 1210 564 170 3478 Fragments arranged by size: 3478 1210 564 170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2101 2645 2101 Size: 544 4878 SacI G_AGCT'C Cuts at: 2109 2709 2109 Size: 600 4822 SacII CC_GC'GG Cuts at: 2094 2278 2094 Size: 184 5238 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2237 Size: 5422 SbfI CC_TGCA'GG Cuts at: 2040 2774 2040 Size: 734 4688 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2036 2600 2770 3905 4586 4777 826 Size: 219 991 564 170 1135 681 191 1471 Fragments arranged by size: 1471 1135 991 681 564 219 191 170 SfiI GGCCn_nnn'nGGCC Cuts at: 2378 2378 Size: 5422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2257 2780 1132 Size: 1125 523 3774 Fragments arranged by size: 3774 1125 523 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2468 2861 513 Size: 552 939 464 393 3074 Fragments arranged by size: 3074 939 552 464 393 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5382 5414 5382 Size: 32 5390 Tth111I GACn'n_nGTC Cuts at: 1316 2294 1316 Size: 978 4444 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2566 3230 276 Size: 53 83 186 936 1032 664 2468 Fragments arranged by size: 2468 1032 936 664 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfoI SrfI SwaI XcmI