Restriction map for
pFN21AE0365
(Linear) (Six-base) MAPSORT of: KIEE0365.seq Check: 6748 from: 1 to: 1560
KIEE0365.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 499 606 1105 1174 1560
Size: 403 96 107 499 69 386
Fragments arranged by size:
499 403 386 107 96 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 802 1560
Size: 802 758
AlwNI CAG_nnn'CTG
Cuts at: 0 560 776 964 1309 1400 1560
Size: 560 216 188 345 91 160
Fragments arranged by size:
560 345 216 188 160 91
ApaI G_GGCC'C
Cuts at: 0 280 1394 1560
Size: 280 1114 166
Fragments arranged by size:
1114 280 166
ApaLI G'TGCA_C
Cuts at: 0 787 1560
Size: 787 773
BanI G'GyrC_C
Cuts at: 0 821 1037 1560
Size: 821 216 523
Fragments arranged by size:
821 523 216
BanII G_rGCy'C
Cuts at: 0 280 407 683 1394 1560
Size: 280 127 276 711 166
Fragments arranged by size:
711 280 276 166 127
BbeI G_GCGC'C
Cuts at: 0 825 1560
Size: 825 735
BbsI GAAGACnn'nnnn_
Cuts at: 0 360 1560
Size: 360 1200
BfrBI ATG'CAT
Cuts at: 0 522 617 1560
Size: 522 95 943
Fragments arranged by size:
943 522 95
BlpI GC'TnA_GC
Cuts at: 0 10 910 1560
Size: 10 900 650
Fragments arranged by size:
900 650 10
Bme1580I G_kGCm'C
Cuts at: 0 280 690 791 1394 1560
Size: 280 410 101 603 166
Fragments arranged by size:
603 410 280 166 101
BmgBI CAC'GTC
Cuts at: 0 1215 1560
Size: 1215 345
BmrI ACTGGGnnnn_n'
Cuts at: 0 647 1122 1560
Size: 647 475 438
Fragments arranged by size:
647 475 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1560
Size: 240 1320
BsaHI Gr'CG_yC
Cuts at: 0 454 822 1501 1560
Size: 454 368 679 59
Fragments arranged by size:
679 454 368 59
BsaWI w'CCGG_w
Cuts at: 0 143 719 1560
Size: 143 576 841
Fragments arranged by size:
841 576 143
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 1560
Size: 226 1334
BseYI C'CCAG_C
Cuts at: 0 24 233 393 1560
Size: 24 209 160 1167
Fragments arranged by size:
1167 209 160 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1188 1560
Size: 1188 372
BsiEI CG_ry'CG
Cuts at: 0 135 1560
Size: 135 1425
BsiHKAI G_wGCw'C
Cuts at: 0 581 683 791 905 1223 1560
Size: 581 102 108 114 318 337
Fragments arranged by size:
581 337 318 114 108 102
Bsp1286I G_dGCh'C
Cuts at: 0 280 407 581 683 690 791 905 1223
Size: 280 127 174 102 7 101 114 318
Cuts at: 1223 1394 1560
Size: 171 166
Fragments arranged by size:
318 280 174 171 166 127 114 102
101 7
BsrBI CCG'CTC
Cuts at: 0 819 1560
Size: 819 741
BsrFI r'CCGG_y
Cuts at: 0 36 1061 1560
Size: 36 1025 499
Fragments arranged by size:
1025 499 36
BsrGI T'GTAC_A
Cuts at: 0 1022 1560
Size: 1022 538
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1071 1560
Size: 1071 489
BstEII G'GTnAC_C
Cuts at: 0 184 1358 1560
Size: 184 1174 202
Fragments arranged by size:
1174 202 184
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 1560
Size: 885 675
Bsu36I CC'TnA_GG
Cuts at: 0 31 1310 1560
Size: 31 1279 250
Fragments arranged by size:
1279 250 31
BtgI C'CryG_G
Cuts at: 0 52 545 995 1197 1560
Size: 52 493 450 202 363
Fragments arranged by size:
493 450 363 202 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1435 1560
Size: 1435 125
DrdI GACnn_nn'nnGTC
Cuts at: 0 440 1234 1312 1560
Size: 440 794 78 248
Fragments arranged by size:
794 440 248 78
EaeI y'GGCC_r
Cuts at: 0 132 242 542 998 1300 1560
Size: 132 110 300 456 302 260
Fragments arranged by size:
456 302 300 260 132 110
EagI C'GGCC_G
Cuts at: 0 132 1560
Size: 132 1428
EarI CTCTTCn'nnn_
Cuts at: 0 1348 1560
Size: 1348 212
EcoICRI GAG'CTC
Cuts at: 0 681 1560
Size: 681 879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 499 606 1105 1174 1560
Size: 403 96 107 499 69 386
Fragments arranged by size:
499 403 386 107 96 69
EcoO109I rG'GnC_Cy
Cuts at: 0 77 225 277 773 1390 1560
Size: 77 148 52 496 617 170
Fragments arranged by size:
617 496 170 148 77 52
HaeII r_GCGC'y
Cuts at: 0 765 825 1450 1560
Size: 765 60 625 110
Fragments arranged by size:
765 625 110 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 1560
Size: 314 32 1214
Fragments arranged by size:
1214 314 32
KasI G'GCGC_C
Cuts at: 0 821 1560
Size: 821 739
MfeI C'AATT_G
Cuts at: 0 636 1560
Size: 636 924
MscI TGG'CCA
Cuts at: 0 244 544 1302 1560
Size: 244 300 758 258
Fragments arranged by size:
758 300 258 244
MslI CAynn'nnrTG
Cuts at: 0 51 973 1560
Size: 51 922 587
Fragments arranged by size:
922 587 51
MspA1I CmG'CkG
Cuts at: 0 237 1199 1560
Size: 237 962 361
Fragments arranged by size:
962 361 237
NaeI GCC'GGC
Cuts at: 0 1063 1560
Size: 1063 497
NarI GG'CG_CC
Cuts at: 0 822 1560
Size: 822 738
NcoI C'CATG_G
Cuts at: 0 545 1560
Size: 545 1015
NgoMIV G'CCGG_C
Cuts at: 0 1061 1560
Size: 1061 499
NruI TCG'CGA
Cuts at: 0 1033 1420 1560
Size: 1033 387 140
Fragments arranged by size:
1033 387 140
NsiI A_TGCA'T
Cuts at: 0 524 619 1560
Size: 524 95 941
Fragments arranged by size:
941 524 95
NspI r_CATG'y
Cuts at: 0 526 1560
Size: 526 1034
PasI CC'CwG_GG
Cuts at: 0 255 1554 1560
Size: 255 1299 6
Fragments arranged by size:
1299 255 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1215 1560
Size: 240 975 345
Fragments arranged by size:
975 345 240
PfoI T'CCnGG_A
Cuts at: 0 812 1560
Size: 812 748
PpuMI rG'GwC_Cy
Cuts at: 0 225 1560
Size: 225 1335
PspOMI G'GGCC_C
Cuts at: 0 276 1390 1560
Size: 276 1114 170
Fragments arranged by size:
1114 276 170
PstI C_TGCA'G
Cuts at: 0 611 781 1560
Size: 611 170 779
Fragments arranged by size:
779 611 170
PvuII CAG'CTG
Cuts at: 0 237 1560
Size: 237 1323
SacI G_AGCT'C
Cuts at: 0 683 1560
Size: 683 877
SacII CC_GC'GG
Cuts at: 0 1200 1560
Size: 1200 360
SfcI C'TryA_G
Cuts at: 0 607 777 1560
Size: 607 170 783
Fragments arranged by size:
783 607 170
SfoI GGC'GCC
Cuts at: 0 823 1560
Size: 823 737
SmlI C'TyrA_G
Cuts at: 0 219 1560
Size: 219 1341
SphI G_CATG'C
Cuts at: 0 526 1560
Size: 526 1034
StyI C'CwwG_G
Cuts at: 0 545 866 1560
Size: 545 321 694
Fragments arranged by size:
694 545 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1055 1222 1560
Size: 1055 167 338
Fragments arranged by size:
1055 338 167
TatI w'GTAC_w
Cuts at: 0 737 1022 1560
Size: 737 285 538
Fragments arranged by size:
737 538 285
Tth111I GACn'n_nGTC
Cuts at: 0 316 364 1560
Size: 316 48 1196
Fragments arranged by size:
1196 316 48
XmnI GAAnn'nnTTC
Cuts at: 0 175 1560
Size: 175 1385
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI BanI BanII BbeI
BbsI BfrBI BlpI Bme1580I BmgBI BmrI BpuEI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI
BsrFI BsrGI BstAPI BstEII BstXI Bsu36I BtgI BtgZI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MfeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SfcI SfoI
SmlI SphI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BglI BglII BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BssHII BssSI BstBI BstYI BstZ17I BtsI ClaI
CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
MmeI NdeI NheI NotI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI XmaI ZraI