(Circular) (Six-base) MAPSORT of: pFN21AB8184.seq Check: 5682 from: 1 to: 5671 pFN21AB8184 5671 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2585 2672 2585 Size: 87 5584 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3481 278 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2540 3734 5118 5386 1632 Size: 908 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 908 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 4570 5547 1203 Size: 3367 977 1327 Fragments arranged by size: 3367 1327 977 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 2415 1166 Size: 1249 4422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2514 1184 Size: 1330 4341 AlwNI CAG_nnn'CTG Cuts at: 2336 3414 4986 2336 Size: 1078 1572 3021 Fragments arranged by size: 3021 1572 1078 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2879 2980 3206 3358 5467 1493 Size: 1386 101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2996 1870 Size: 73 228 825 4545 Fragments arranged by size: 4545 825 228 73 BamHI G'GATC_C Cuts at: 3001 3368 3001 Size: 367 5304 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2597 2992 4447 618 Size: 325 217 627 810 395 1455 1842 Fragments arranged by size: 1842 1455 810 627 395 325 217 BanII G_rGCy'C Cuts at: 1391 2208 2990 1391 Size: 817 782 4072 Fragments arranged by size: 4072 817 782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2546 2580 3862 3896 1903 Size: 34 609 34 1282 34 3678 Fragments arranged by size: 3678 1282 609 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2150 2324 2812 Size: 107 122 71 631 605 478 174 488 Cuts at: 2812 4282 136 Size: 1470 1525 Fragments arranged by size: 1525 1470 631 605 488 478 174 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 2438 3088 2438 Size: 650 5021 Bme1580I G_kGCm'C Cuts at: 1790 3732 4888 1790 Size: 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3402 4360 442 Size: 416 53 83 399 24 1985 958 1753 Fragments arranged by size: 1985 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2770 2770 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2945 4331 1239 Size: 1706 1386 2579 Fragments arranged by size: 2579 1706 1386 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3796 4661 4959 3796 Size: 865 298 4508 Fragments arranged by size: 4508 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3462 493 Size: 711 2258 2702 Fragments arranged by size: 2702 2258 711 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3478 3860 4495 275 Size: 53 83 186 936 1945 382 635 1451 Fragments arranged by size: 1945 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2958 4104 4502 4776 4923 5334 2958 Size: 1146 398 274 147 411 3295 Fragments arranged by size: 3295 1146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5446 Fragments arranged by size: 5446 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5671 BseYI C'CCAG_C Cuts at: 4874 5370 4874 Size: 496 5175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2139 2552 2693 2857 1163 Size: 573 403 413 141 164 3977 Fragments arranged by size: 3977 573 413 403 164 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3732 3817 4888 3732 Size: 85 1071 4515 Fragments arranged by size: 4515 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2990 3732 3817 4888 1391 Size: 399 418 782 742 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 418 399 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2585 2672 3026 844 Size: 1741 87 354 3489 Fragments arranged by size: 3489 1741 354 87 BsrBI CCG'CTC Cuts at: 1394 1823 2190 2229 2239 2452 3558 1394 Size: 429 367 39 10 213 1106 3507 Fragments arranged by size: 3507 1106 429 367 213 39 10 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2811 4315 4502 1700 Size: 110 226 775 1504 187 2869 Fragments arranged by size: 2869 1504 775 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 2703 2802 1925 Size: 778 99 4794 Fragments arranged by size: 4794 778 99 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 2336 2826 794 Size: 1542 490 3639 Fragments arranged by size: 3639 1542 490 BstBI TT'CG_AA Cuts at: 4475 4475 Size: 5671 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2464 2383 Size: 81 5590 BstYI r'GATC_y Cuts at: 1972 3001 3368 3753 3770 5222 5308 5320 5666 Size: 1029 367 385 17 1452 86 12 346 Cuts at: 5666 1972 Size: 1977 Fragments arranged by size: 1977 1452 1029 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2376 2457 513 Size: 552 1311 81 3727 Fragments arranged by size: 3727 1311 552 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2092 2127 2272 2319 2840 3187 3981 4001 Size: 9 35 145 47 521 347 794 20 Cuts at: 4001 2083 Size: 3753 Fragments arranged by size: 3753 794 521 347 145 47 35 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2349 2373 2427 Size: 54 1425 50 275 18 519 24 54 Cuts at: 2427 2614 2694 2863 3118 4007 5636 8 Size: 187 80 169 255 889 1629 43 Fragments arranged by size: 1629 1425 889 519 275 255 187 169 80 54 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2257 2669 3601 1353 Size: 904 412 932 3423 Fragments arranged by size: 3423 932 904 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2313 4258 4632 4778 387 Size: 1064 138 724 1945 374 146 1280 Fragments arranged by size: 1945 1280 1064 724 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2540 2945 3734 4331 5118 5386 1239 Size: 393 908 405 789 597 787 268 1524 Fragments arranged by size: 1524 908 789 787 597 405 393 268 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FspI TGC'GCA Cuts at: 2502 4177 2502 Size: 1675 3996 HaeII r_GCGC'y Cuts at: 1678 2643 4818 1678 Size: 965 2175 2531 Fragments arranged by size: 2531 2175 965 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2851 2883 4312 4344 4386 Size: 32 135 32 1279 32 1429 32 42 Cuts at: 4386 4418 1373 Size: 32 2626 Fragments arranged by size: 2626 1429 1279 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2159 2646 2966 4785 4969 1305 Size: 854 487 320 1819 184 2007 Fragments arranged by size: 2007 1819 854 487 320 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2351 2429 2616 2865 10 Size: 54 1425 343 519 78 187 249 2816 Fragments arranged by size: 2816 1425 519 343 249 187 78 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2415 2513 3629 3988 4147 518 Size: 648 1215 34 98 1116 359 159 2042 Fragments arranged by size: 2042 1215 1116 648 359 159 98 34 MspA1I CmG'CkG Cuts at: 1606 2070 2336 2482 2548 2918 3087 3764 4912 Size: 464 266 146 66 370 169 677 1148 Cuts at: 4912 5157 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 464 370 266 245 169 146 66 NaeI GCC'GGC Cuts at: 2038 2813 2038 Size: 775 4896 NcoI C'CATG_G Cuts at: 513 1065 2376 513 Size: 552 1311 3808 Fragments arranged by size: 3808 1311 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NgoMIV G'CCGG_C Cuts at: 2036 2811 2036 Size: 775 4896 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2800 3029 4574 1132 Size: 97 1571 229 1545 2229 Fragments arranged by size: 2229 1571 1545 229 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 4570 4570 Size: 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PstI C_TGCA'G Cuts at: 830 2332 2493 3023 830 Size: 1502 161 530 3478 Fragments arranged by size: 3478 1502 530 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 PvuII CAG'CTG Cuts at: 2482 2482 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 2328 2489 3019 4154 4835 5026 826 Size: 219 1283 161 530 1135 681 191 1471 Fragments arranged by size: 1471 1283 1135 681 530 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3775 4676 4938 820 Size: 197 926 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3462 493 Size: 2969 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 2661 1517 Size: 1144 4527 StyI C'CwwG_G Cuts at: 513 1065 2376 3110 513 Size: 552 1311 734 3074 Fragments arranged by size: 3074 1311 734 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3698 3715 3868 4053 4498 325 Size: 1255 2118 17 153 185 445 1498 Fragments arranged by size: 2118 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5631 5663 5631 Size: 32 5639 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5671 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3479 276 Size: 53 83 186 936 1945 2468 Fragments arranged by size: 2468 1945 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI