Restriction map for pFN21AB8184
 (Circular) (Six-base) MAPSORT of: pFN21AB8184.seq  Check: 5682  from: 1  to: 5671



pFN21AB8184  5671 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2585    2672    2585 
   Size:         87    5584
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3481     278 
   Size:         53      83     186     936    1945    2468

  Fragments arranged by size: 

                2468    1945     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3014    1937 
   Size:       1077    4594
 
Acc65I G'GTAC_C

Cuts at:   2992    2992 
   Size:       5671
 
AclI AA'CG_TT

Cuts at:   1563    3798    4171    1563 
   Size:       2235     373    3063

  Fragments arranged by size: 

                3063    2235     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2540    3734    5118    5386    1632 
   Size:        908    1194    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1194     908     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5474
 
AflIII A'CryG_T

Cuts at:   1203    4570    5547    1203 
   Size:       3367     977    1327

  Fragments arranged by size: 

                3367    1327     977
 
AgeI A'CCGG_T

Cuts at:   4502    4502 
   Size:       5671
 
AhdI GACnn_n'nnGTC

Cuts at:   4400    4400 
   Size:       5671
 
AleI CACnn'nnGTG

Cuts at:   1166    2415    1166 
   Size:       1249    4422
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2514    1184 
   Size:       1330    4341
 
AlwNI CAG_nnn'CTG

Cuts at:   2336    3414    4986    2336 
   Size:       1078    1572    3021

  Fragments arranged by size: 

                3021    1572    1078
 
ApaLI G'TGCA_C

Cuts at:   3728    4884    3728 
   Size:       1156    4515
 
ApoI r'AATT_y

Cuts at:   1493    2879    2980    3206    3358    5467    1493 
   Size:       1386     101     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1386     226     152     101
 
AseI AT'TA_AT

Cuts at:    160    1032    4225     160 
   Size:        872    3193    1606

  Fragments arranged by size: 

                3193    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5671
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2171    2996    1870 
   Size:         73     228     825    4545

  Fragments arranged by size: 

                4545     825     228      73
 
BamHI G'GATC_C

Cuts at:   3001    3368    3001 
   Size:        367    5304
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2597    2992    4447     618 
   Size:        325     217     627     810     395    1455    1842

  Fragments arranged by size: 

                1842    1455     810     627     395     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2208    2990    1391 
   Size:        817     782    4072

  Fragments arranged by size: 

                4072     817     782
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4700
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2546    2580    3862    3896    1903 
   Size:         34     609      34    1282      34    3678

  Fragments arranged by size: 

                3678    1282     609      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3563    4773    3563 
   Size:       1210    4461
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5671
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2150    2324    2812 
   Size:        107     122      71     631     605     478     174     488

Cuts at:   2812    4282     136 
   Size:       1470    1525

  Fragments arranged by size: 

                1525    1470     631     605     488     478     174     122
                 107      71
 
BglII A'GATC_T

Cuts at:   5666    5666 
   Size:       5671
 
BlpI GC'TnA_GC

Cuts at:   2438    3088    2438 
   Size:        650    5021
 
Bme1580I G_kGCm'C

Cuts at:   1790    3732    4888    1790 
   Size:       1942    1156    2573

  Fragments arranged by size: 

                2573    1942    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5671
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3402    4360     442 
   Size:        416      53      83     399      24    1985     958    1753

  Fragments arranged by size: 

                1985    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5671
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2770    2770 
   Size:       5671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2945    4331    1239 
   Size:       1706    1386    2579

  Fragments arranged by size: 

                2579    1706    1386
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3796    4661    4959    3796 
   Size:        865     298    4508

  Fragments arranged by size: 

                4508     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4334     882 
   Size:        617    2835    2219

  Fragments arranged by size: 

                2835    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3462     493 
   Size:        711    2258    2702

  Fragments arranged by size: 

                2702    2258     711
 
BsaBI GATnn'nnATC

Cuts at:   5665    5665 
   Size:       5671
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3478    3860    4495     275 
   Size:         53      83     186     936    1945     382     635    1451

  Fragments arranged by size: 

                1945    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2958    4104    4502    4776    4923    5334    2958 
   Size:       1146     398     274     147     411    3295

  Fragments arranged by size: 

                3295    1146     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5446

  Fragments arranged by size: 

                5446     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5671
 
BseYI C'CCAG_C

Cuts at:   4874    5370    4874 
   Size:        496    5175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2139    2552    2693    2857    1163 
   Size:        573     403     413     141     164    3977

  Fragments arranged by size: 

                3977     573     413     403     164     141
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3121    3882    4910    1540 
   Size:        275     186    1120     761    1028    2301

  Fragments arranged by size: 

                2301    1120    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3732    3817    4888    3732 
   Size:         85    1071    4515

  Fragments arranged by size: 

                4515    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3182    3275    3182 
   Size:         93    5578
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2208    2990    3732    3817    4888    1391 
   Size:        399     418     782     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     782     742     418     399      85
 
BspEI T'CCGG_A

Cuts at:   5334    5334 
   Size:       5671
 
BspHI T'CATG_A

Cuts at:   3560    5290    3560 
   Size:       1730    3941
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2585    2672    3026     844 
   Size:       1741      87     354    3489

  Fragments arranged by size: 

                3489    1741     354      87
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2190    2229    2239    2452    3558    1394 
   Size:        429     367      39      10     213    1106    3507

  Fragments arranged by size: 

                3507    1106     429     367     213      39      10
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4166    4340      65 
   Size:       1183    2918     174    1396

  Fragments arranged by size: 

                2918    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2036    2811    4315    4502    1700 
   Size:        110     226     775    1504     187    2869

  Fragments arranged by size: 

                2869    1504     775     226     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5671
 
BssHII G'CGCG_C

Cuts at:   1925    2703    2802    1925 
   Size:        778      99    4794

  Fragments arranged by size: 

                4794     778      99
 
BssSI C'ACGA_G

Cuts at:   3731    4743    3731 
   Size:       1012    4659
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2336    2826     794 
   Size:       1542     490    3639

  Fragments arranged by size: 

                3639    1542     490
 
BstBI TT'CG_AA

Cuts at:   4475    4475 
   Size:       5671
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5671
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2383    2464    2383 
   Size:         81    5590
 
BstYI r'GATC_y

Cuts at:   1972    3001    3368    3753    3770    5222    5308    5320    5666 
   Size:       1029     367     385      17    1452      86      12     346

Cuts at:   5666    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1029     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2376    2457     513 
   Size:        552    1311      81    3727

  Fragments arranged by size: 

                3727    1311     552      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4932

  Fragments arranged by size: 

                4932     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2083    2092    2127    2272    2319    2840    3187    3981    4001 
   Size:          9      35     145      47     521     347     794      20

Cuts at:   4001    2083 
   Size:       3753

  Fragments arranged by size: 

                3753     794     521     347     145      47      35      20
                   9
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5671
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5636
 
DraI TTT'AAA

Cuts at:   2975    2975 
   Size:       5671
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4678     809 
   Size:        507    3362    1802

  Fragments arranged by size: 

                3362    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2349    2373    2427 
   Size:         54    1425      50     275      18     519      24      54

Cuts at:   2427    2614    2694    2863    3118    4007    5636       8 
   Size:        187      80     169     255     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     519     275     255     187     169
                  80      54      54      50      43      24      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3118    1537 
   Size:        275    1306    4090

  Fragments arranged by size: 

                4090    1306     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2257    2669    3601    1353 
   Size:        904     412     932    3423

  Fragments arranged by size: 

                3423     932     904     412
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2313    4258    4632    4778     387 
   Size:       1064     138     724    1945     374     146    1280

  Fragments arranged by size: 

                1945    1280    1064     724     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2540    2945    3734    4331    5118    5386    1239 
   Size:        393     908     405     789     597     787     268    1524

  Fragments arranged by size: 

                1524     908     789     787     597     405     393     268
            
 
EcoRI G'AATT_C

Cuts at:   2980    3358    2980 
   Size:        378    5293
 
FspI TGC'GCA

Cuts at:   2502    4177    2502 
   Size:       1675    3996
 
HaeII r_GCGC'y

Cuts at:   1678    2643    4818    1678 
   Size:        965    2175    2531

  Fragments arranged by size: 

                2531    2175     965
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2851    2883    4312    4344    4386 
   Size:         32     135      32    1279      32    1429      32      42

Cuts at:   4386    4418    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1429    1279     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3015    3261     669 
   Size:        988     281    1077     246    3079

  Fragments arranged by size: 

                3079    1077     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5671
 
HpaI GTT'AAC

Cuts at:   3261    3261 
   Size:       5671
 
KpnI G_GTAC'C

Cuts at:   2996    2996 
   Size:       5671
 
MfeI C'AATT_G

Cuts at:   3270    3270 
   Size:       5671
 
MluI A'CGCG_T

Cuts at:   5547    5547 
   Size:       5671
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2159    2646    2966    4785    4969    1305 
   Size:        854     487     320    1819     184    2007

  Fragments arranged by size: 

                2007    1819     854     487     320     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2351    2429    2616    2865      10 
   Size:         54    1425     343     519      78     187     249    2816

  Fragments arranged by size: 

                2816    1425     519     343     249     187      78      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2381    2415    2513    3629    3988    4147     518 
   Size:        648    1215      34      98    1116     359     159    2042

  Fragments arranged by size: 

                2042    1215    1116     648     359     159      98      34
            
 
MspA1I CmG'CkG

Cuts at:   1606    2070    2336    2482    2548    2918    3087    3764    4912 
   Size:        464     266     146      66     370     169     677    1148

Cuts at:   4912    5157    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     677     464     370     266     245     169
                 146      66
 
NaeI GCC'GGC

Cuts at:   2038    2813    2038 
   Size:        775    4896
 
NcoI C'CATG_G

Cuts at:    513    1065    2376     513 
   Size:        552    1311    3808

  Fragments arranged by size: 

                3808    1311     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5671
 
NgoMIV G'CCGG_C

Cuts at:   2036    2811    2036 
   Size:        775    4896
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5671
 
NotI GC'GGCC_GC

Cuts at:   3118    3118 
   Size:       5671
 
NspI r_CATG'y

Cuts at:   1132    1229    2800    3029    4574    1132 
   Size:         97    1571     229    1545    2229

  Fragments arranged by size: 

                2229    1571    1545     229      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5671
 
PciI A'CATG_T

Cuts at:   4570    4570 
   Size:       5671
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5671
 
PmeI GTTT'AAAC

Cuts at:   2975    2975 
   Size:       5671
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3740    3772    5253    5285    3740 
   Size:         32    1481      32    4126

  Fragments arranged by size: 

                4126    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5671
 
PsiI TTA'TAA

Cuts at:   3241    3241 
   Size:       5671
 
PstI C_TGCA'G

Cuts at:    830    2332    2493    3023     830 
   Size:       1502     161     530    3478

  Fragments arranged by size: 

                3478    1502     530     161
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5671
 
PvuII CAG'CTG

Cuts at:   2482    2482 
   Size:       5671
 
SalI G'TCGA_C

Cuts at:   1936    3013    1936 
   Size:       1077    4594
 
SbfI CC_TGCA'GG

Cuts at:   3023    3023 
   Size:       5671
 
ScaI AGT'ACT

Cuts at:   3919    5631    3919 
   Size:       1712    3959
 
SfcI C'TryA_G

Cuts at:    826    1045    2328    2489    3019    4154    4835    5026     826 
   Size:        219    1283     161     530    1135     681     191    1471

  Fragments arranged by size: 

                1471    1283    1135     681     530     219     191     161
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5671
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5671
 
SmaI CCC'GGG

Cuts at:   1872    2998    1872 
   Size:       1126    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3775    4676    4938     820 
   Size:        197     926    1832     901     262    1553

  Fragments arranged by size: 

                1832    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3462     493 
   Size:       2969    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5671
 
SphI G_CATG'C

Cuts at:   1132    3029    1132 
   Size:       1897    3774
 
SspI AAT'ATT

Cuts at:      5      52    3595       5 
   Size:         47    3543    2081

  Fragments arranged by size: 

                3543    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2661    1517 
   Size:       1144    4527
 
StyI C'CwwG_G

Cuts at:    513    1065    2376    3110     513 
   Size:        552    1311     734    3074

  Fragments arranged by size: 

                3074    1311     734     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3698    3715    3868    4053    4498     325 
   Size:       1255    2118      17     153     185     445    1498

  Fragments arranged by size: 

                2118    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3917    5629      96 
   Size:        274      80      33      51    1443    1940    1712     138

  Fragments arranged by size: 

                1940    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5631    5663    5631 
   Size:         32    5639
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5671
 
XbaI T'CTAG_A

Cuts at:   3007    3007 
   Size:       5671
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5671
 
XmaI C'CCGG_G

Cuts at:   1870    2996    1870 
   Size:       1126    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3800    3800 
   Size:       5671
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3479     276 
   Size:         53      83     186     936    1945    2468

  Fragments arranged by size: 

                2468    1945     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NarI
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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