Restriction map for
pFN21AB8184
(Linear) (Six-base) MAPSORT of: KIBB8184.seq Check: 8430 from: 1 to: 966
KIBB8184.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 580 667 966
Size: 580 87 299
Fragments arranged by size:
580 299 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 966
Size: 535 431
AleI CACnn'nnGTG
Cuts at: 0 410 966
Size: 410 556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 966
Size: 509 457
AlwNI CAG_nnn'CTG
Cuts at: 0 331 966
Size: 331 635
ApoI r'AATT_y
Cuts at: 0 874 966
Size: 874 92
AvaI C'yCGr_G
Cuts at: 0 166 966
Size: 166 800
BanI G'GyrC_C
Cuts at: 0 592 966
Size: 592 374
BanII G_rGCy'C
Cuts at: 0 203 966
Size: 203 763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 575 966
Size: 541 34 391
Fragments arranged by size:
541 391 34
BglI GCCn_nnn'nGGC
Cuts at: 0 145 319 807 966
Size: 145 174 488 159
Fragments arranged by size:
488 174 159 145
BlpI GC'TnA_GC
Cuts at: 0 433 966
Size: 433 533
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 765 966
Size: 765 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 966
Size: 940 26
BsaWI w'CCGG_w
Cuts at: 0 953 966
Size: 953 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 547 688 852 966
Size: 134 413 141 164 114
Fragments arranged by size:
413 164 141 134 114
Bsp1286I G_dGCh'C
Cuts at: 0 203 966
Size: 203 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 580 667 966
Size: 580 87 299
Fragments arranged by size:
580 299 87
BsrBI CCG'CTC
Cuts at: 0 185 224 234 447 966
Size: 185 39 10 213 519
Fragments arranged by size:
519 213 185 39 10
BsrFI r'CCGG_y
Cuts at: 0 31 806 966
Size: 31 775 160
Fragments arranged by size:
775 160 31
BssHII G'CGCG_C
Cuts at: 0 698 797 966
Size: 698 99 169
Fragments arranged by size:
698 169 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 331 821 966
Size: 331 490 145
Fragments arranged by size:
490 331 145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 459 966
Size: 378 81 507
Fragments arranged by size:
507 378 81
BtgI C'CryG_G
Cuts at: 0 371 452 966
Size: 371 81 514
Fragments arranged by size:
514 371 81
BtsI GCAGTG_nn'
Cuts at: 0 78 87 122 267 314 835 966
Size: 78 9 35 145 47 521 131
Fragments arranged by size:
521 145 131 78 47 35 9
EaeI y'GGCC_r
Cuts at: 0 344 368 422 609 689 858 966
Size: 344 24 54 187 80 169 108
Fragments arranged by size:
344 187 169 108 80 54 24
EarI CTCTTCn'nnn_
Cuts at: 0 252 664 966
Size: 252 412 302
Fragments arranged by size:
412 302 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 966
Size: 308 658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 940 966
Size: 535 405 26
Fragments arranged by size:
535 405 26
FspI TGC'GCA
Cuts at: 0 497 966
Size: 497 469
HaeII r_GCGC'y
Cuts at: 0 638 966
Size: 638 328
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 846 878 966
Size: 846 32 88
Fragments arranged by size:
846 88 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 641 961 966
Size: 154 487 320 5
Fragments arranged by size:
487 320 154 5
MscI TGG'CCA
Cuts at: 0 346 424 611 860 966
Size: 346 78 187 249 106
Fragments arranged by size:
346 249 187 106 78
MslI CAynn'nnrTG
Cuts at: 0 376 410 508 966
Size: 376 34 98 458
Fragments arranged by size:
458 376 98 34
MspA1I CmG'CkG
Cuts at: 0 65 331 477 543 913 966
Size: 65 266 146 66 370 53
Fragments arranged by size:
370 266 146 66 65 53
NaeI GCC'GGC
Cuts at: 0 33 808 966
Size: 33 775 158
Fragments arranged by size:
775 158 33
NcoI C'CATG_G
Cuts at: 0 371 966
Size: 371 595
NgoMIV G'CCGG_C
Cuts at: 0 31 806 966
Size: 31 775 160
Fragments arranged by size:
775 160 31
NspI r_CATG'y
Cuts at: 0 795 966
Size: 795 171
PstI C_TGCA'G
Cuts at: 0 327 488 966
Size: 327 161 478
Fragments arranged by size:
478 327 161
PvuII CAG'CTG
Cuts at: 0 477 966
Size: 477 489
SfcI C'TryA_G
Cuts at: 0 323 484 966
Size: 323 161 482
Fragments arranged by size:
482 323 161
StuI AGG'CCT
Cuts at: 0 656 966
Size: 656 310
StyI C'CwwG_G
Cuts at: 0 371 966
Size: 371 595
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApoI AvaI BanI
BanII BcgI BglI BlpI BplI BpmI BsaWI BsgI
Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BtgI
BtsI EaeI EarI EciI Eco57MI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PstI PvuII SfcI StuI StyI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI