(Circular) (Six-base) MAPSORT of: pFN21AB8598.seq Check: 1803 from: 1 to: 7033 pFN21AB8598 7033 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2083 2083 Size: 7033 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4843 278 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3815 4376 1937 Size: 1878 561 4594 Fragments arranged by size: 4594 1878 561 Acc65I G'GTAC_C Cuts at: 4354 4354 Size: 7033 AclI AA'CG_TT Cuts at: 1563 5160 5533 1563 Size: 3597 373 3063 Fragments arranged by size: 3597 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2060 3500 3824 5096 6480 6748 1632 Size: 428 1440 324 1272 1384 268 1917 Fragments arranged by size: 1917 1440 1384 1272 428 324 268 AflII C'TTAA_G Cuts at: 820 1017 2279 2315 820 Size: 197 1262 36 5538 Fragments arranged by size: 5538 1262 197 36 AflIII A'CryG_T Cuts at: 1203 5932 6909 1203 Size: 4729 977 1327 Fragments arranged by size: 4729 1327 977 AgeI A'CCGG_T Cuts at: 5864 5864 Size: 7033 AhdI GACnn_n'nnGTC Cuts at: 5762 5762 Size: 7033 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3635 1184 Size: 2451 4582 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2187 2219 2187 Size: 32 7001 AlwNI CAG_nnn'CTG Cuts at: 2777 3877 4776 6348 2777 Size: 1100 899 1572 3462 Fragments arranged by size: 3462 1572 1100 899 ApaI G_GGCC'C Cuts at: 2672 2672 Size: 7033 ApaLI G'TGCA_C Cuts at: 5090 6246 5090 Size: 1156 5877 ApoI r'AATT_y Cuts at: 1493 2285 2354 2409 2753 2907 2921 2929 4342 Size: 792 69 55 344 154 14 8 1413 Cuts at: 4342 4568 4720 6829 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1413 792 344 226 154 152 69 55 14 8 AseI AT'TA_AT Cuts at: 160 1032 5587 160 Size: 872 4555 1606 Fragments arranged by size: 4555 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 AvaI C'yCGr_G Cuts at: 1870 1943 4236 4358 1870 Size: 73 2293 122 4545 Fragments arranged by size: 4545 2293 122 73 AvrII C'CTAG_G Cuts at: 3001 3001 Size: 7033 BamHI G'GATC_C Cuts at: 4363 4730 4363 Size: 367 6666 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4354 5809 618 Size: 325 217 627 2567 1455 1842 Fragments arranged by size: 2567 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2139 2248 2672 3629 4352 1391 Size: 748 109 424 957 723 4072 Fragments arranged by size: 4072 957 748 723 424 109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3339 3352 3381 4125 928 Size: 971 1440 13 29 744 3836 Fragments arranged by size: 3836 1440 971 744 29 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5224 5258 1903 Size: 34 3287 34 3678 Fragments arranged by size: 3678 3287 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4925 6135 4925 Size: 1210 5823 BclI T'GATC_A Cuts at: 1548 4248 1548 Size: 2700 4333 BfrBI ATG'CAT Cuts at: 2657 4260 2657 Size: 1603 5430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3729 5644 136 Size: 107 122 71 631 605 2057 1915 1525 Fragments arranged by size: 2057 1915 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2061 3550 7028 2061 Size: 1489 3478 2066 Fragments arranged by size: 3478 2066 1489 BlpI GC'TnA_GC Cuts at: 3623 4450 3623 Size: 827 6206 Bme1580I G_kGCm'C Cuts at: 1790 2672 5094 6250 1790 Size: 882 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 882 BmgBI CAC'GTC Cuts at: 1318 3744 1318 Size: 2426 4607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2765 4764 5722 Size: 416 53 83 399 24 1348 1999 958 Cuts at: 5722 442 Size: 1753 Fragments arranged by size: 1999 1753 1348 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7033 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3523 3523 Size: 7033 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2259 4207 5693 1239 Size: 981 39 1948 1486 2579 Fragments arranged by size: 2579 1948 1486 981 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 5158 6023 6321 2831 Size: 2327 865 298 3543 Fragments arranged by size: 3543 2327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5696 882 Size: 617 4197 2219 Fragments arranged by size: 4197 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2629 4824 493 Size: 711 1425 2195 2702 Fragments arranged by size: 2702 2195 1425 711 BsaBI GATnn'nnATC Cuts at: 3830 7027 3830 Size: 3197 3836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3812 4840 5222 5857 Size: 53 83 186 936 2279 1028 382 635 Cuts at: 5857 275 Size: 1451 Fragments arranged by size: 2279 1451 1028 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5466 5864 6138 6285 6696 5466 Size: 398 274 147 411 5803 Fragments arranged by size: 5803 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2187 2217 2497 2527 3269 Size: 30 165 30 784 30 280 30 742 Cuts at: 3269 3299 3759 3789 1178 Size: 30 460 30 4422 Fragments arranged by size: 4422 784 742 460 280 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2208 2256 3332 1186 Size: 1022 48 1076 4887 Fragments arranged by size: 4887 1076 1022 48 BseYI C'CCAG_C Cuts at: 6236 6732 6236 Size: 496 6537 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3890 1163 Size: 573 2154 4306 Fragments arranged by size: 4306 2154 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4483 5244 6272 1540 Size: 275 186 2482 761 1028 2301 Fragments arranged by size: 2482 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 2248 3629 5094 5179 6250 2139 Size: 109 1381 1465 85 1071 2922 Fragments arranged by size: 2922 1465 1381 1071 109 85 BsmI GAATG_Cn' Cuts at: 3155 3612 4544 4637 3155 Size: 457 932 93 5551 Fragments arranged by size: 5551 932 457 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2248 2672 3629 4352 5094 5179 Size: 399 349 109 424 957 723 742 85 Cuts at: 5179 6250 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 957 742 723 424 399 349 109 85 BspEI T'CCGG_A Cuts at: 6696 6696 Size: 7033 BspHI T'CATG_A Cuts at: 2842 4158 4922 6652 2842 Size: 1316 764 1730 3223 Fragments arranged by size: 3223 1730 1316 764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 4388 844 Size: 1239 2305 3489 Fragments arranged by size: 3489 2305 1239 BsrBI CCG'CTC Cuts at: 1394 1823 4920 1394 Size: 429 3097 3507 Fragments arranged by size: 3507 3097 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5528 5702 65 Size: 1183 4280 174 1396 Fragments arranged by size: 4280 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 5677 5864 1700 Size: 110 271 3596 187 2869 Fragments arranged by size: 3596 2869 271 187 110 BsrGI T'GTAC_A Cuts at: 96 2097 2617 3103 3656 96 Size: 2001 520 486 553 3473 Fragments arranged by size: 3473 2001 553 520 486 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7033 BssSI C'ACGA_G Cuts at: 2190 5093 6105 2190 Size: 2903 1012 3118 Fragments arranged by size: 3118 2903 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7033 BstBI TT'CG_AA Cuts at: 5837 5837 Size: 7033 BstEII G'GTnAC_C Cuts at: 1184 3226 1184 Size: 2042 4991 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2131 Size: 7033 BstYI r'GATC_y Cuts at: 1972 2061 3550 4363 4730 5115 5132 6584 6670 Size: 89 1489 813 367 385 17 1452 86 Cuts at: 6670 6682 7028 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1489 1452 813 385 367 346 89 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2032 513 Size: 552 967 5514 Fragments arranged by size: 5514 967 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6294 Fragments arranged by size: 6294 683 56 BtsI GCAGTG_nn' Cuts at: 4549 5343 5363 4549 Size: 794 20 6219 Fragments arranged by size: 6219 794 20 ClaI AT'CG_AT Cuts at: 1554 2383 1554 Size: 829 6204 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6998 DraI TTT'AAA Cuts at: 2556 3084 4337 2556 Size: 528 1253 5252 Fragments arranged by size: 5252 1253 528 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6040 809 Size: 507 4724 1802 Fragments arranged by size: 4724 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 3730 4143 Size: 54 1425 50 275 18 285 1615 413 Cuts at: 4143 4480 5369 6998 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1615 1425 889 413 337 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4480 1537 Size: 275 2668 4090 Fragments arranged by size: 4090 2668 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2818 4963 1353 Size: 1465 2145 3423 Fragments arranged by size: 3423 2145 1465 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5620 5994 6140 387 Size: 1064 138 4031 374 146 1280 Fragments arranged by size: 4031 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2137 2246 3627 2137 Size: 109 1381 5543 Fragments arranged by size: 5543 1381 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2220 2259 3500 3824 4207 5096 Size: 393 428 160 39 1241 324 383 889 Cuts at: 5096 5693 6480 6748 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1241 889 787 597 428 393 383 324 268 160 39 EcoO109I rG'GnC_Cy Cuts at: 2668 2669 3633 4204 2668 Size: 1 964 571 5497 Fragments arranged by size: 5497 964 571 1 EcoRI G'AATT_C Cuts at: 2753 4342 4720 2753 Size: 1589 378 5066 Fragments arranged by size: 5066 1589 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 7033 FspI TGC'GCA Cuts at: 5539 5539 Size: 7033 HaeII r_GCGC'y Cuts at: 1678 6180 1678 Size: 4502 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2497 2529 3535 Size: 32 135 32 498 32 395 32 1006 Cuts at: 3535 3567 5674 5706 5748 5780 1373 Size: 32 2107 32 42 32 2626 Fragments arranged by size: 2626 2107 1006 498 395 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2013 2679 3816 4051 4377 4623 Size: 988 281 75 666 1137 235 326 246 Cuts at: 4623 669 Size: 3079 Fragments arranged by size: 3079 1137 988 666 326 281 246 235 75 HindIII A'AGCT_T Cuts at: 748 3486 748 Size: 2738 4295 HpaI GTT'AAC Cuts at: 2679 4051 4623 2679 Size: 1372 572 5089 Fragments arranged by size: 5089 1372 572 KpnI G_GTAC'C Cuts at: 4358 4358 Size: 7033 MfeI C'AATT_G Cuts at: 4632 4632 Size: 7033 MluI A'CGCG_T Cuts at: 6909 6909 Size: 7033 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3003 3660 6147 6331 1305 Size: 1698 657 2487 184 2007 Fragments arranged by size: 2487 2007 1698 657 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 4926 Fragments arranged by size: 4926 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 3539 3617 4058 4163 4991 5350 5509 Size: 648 2373 78 441 105 828 359 159 Cuts at: 5509 518 Size: 2042 Fragments arranged by size: 2373 2042 828 648 441 359 159 105 78 MspA1I CmG'CkG Cuts at: 1606 3877 4216 4449 5126 6274 6519 1606 Size: 2271 339 233 677 1148 245 2120 Fragments arranged by size: 2271 2120 1148 677 339 245 233 NaeI GCC'GGC Cuts at: 2083 2083 Size: 7033 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6481 NdeI CA'TA_TG Cuts at: 387 387 Size: 7033 NgoMIV G'CCGG_C Cuts at: 2081 2081 Size: 7033 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7033 NotI GC'GGCC_GC Cuts at: 4480 4480 Size: 7033 NsiI A_TGCA'T Cuts at: 2659 4262 2659 Size: 1603 5430 NspI r_CATG'y Cuts at: 1132 1229 2971 3526 4391 5936 1132 Size: 97 1742 555 865 1545 2229 Fragments arranged by size: 2229 1742 1545 865 555 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7033 PciI A'CATG_T Cuts at: 5932 5932 Size: 7033 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7033 PfoI T'CCnGG_A Cuts at: 2991 2991 Size: 7033 PmeI GTTT'AAAC Cuts at: 4337 4337 Size: 7033 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 2219 2871 2903 5102 5134 6615 6647 2187 Size: 32 652 32 2199 32 1481 32 2573 Fragments arranged by size: 2573 2199 1481 652 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3633 4204 3633 Size: 571 6462 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7033 PsiI TTA'TAA Cuts at: 2154 4029 4603 2154 Size: 1875 574 4584 Fragments arranged by size: 4584 1875 574 PspOMI G'GGCC_C Cuts at: 2668 2668 Size: 7033 PspXI vC'TCGA_Gb Cuts at: 4236 4236 Size: 7033 PstI C_TGCA'G Cuts at: 830 4385 830 Size: 3555 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7033 PvuII CAG'CTG Cuts at: 3877 4216 3877 Size: 339 6694 SacI G_AGCT'C Cuts at: 2139 2248 3629 2139 Size: 109 1381 5543 Fragments arranged by size: 5543 1381 109 SalI G'TCGA_C Cuts at: 1936 3814 4375 1936 Size: 1878 561 4594 Fragments arranged by size: 4594 1878 561 SbfI CC_TGCA'GG Cuts at: 4385 4385 Size: 7033 ScaI AGT'ACT Cuts at: 2749 5281 6993 2749 Size: 2532 1712 2789 Fragments arranged by size: 2789 2532 1712 SfcI C'TryA_G Cuts at: 826 1045 4318 4381 5516 6197 6388 826 Size: 219 3273 63 1135 681 191 1471 Fragments arranged by size: 3273 1471 1135 681 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7033 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7033 SmaI CCC'GGG Cuts at: 1872 4360 1872 Size: 2488 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 2315 2810 4236 5137 6038 Size: 197 926 336 36 495 1426 901 901 Cuts at: 6038 6300 820 Size: 262 1553 Fragments arranged by size: 1553 1426 926 901 901 495 336 262 197 36 SnaBI TAC'GTA Cuts at: 493 4824 493 Size: 4331 2702 SpeI A'CTAG_T Cuts at: 152 2165 152 Size: 2013 5020 SphI G_CATG'C Cuts at: 1132 4391 1132 Size: 3259 3774 SspI AAT'ATT Cuts at: 5 52 4286 4957 5 Size: 47 4234 671 2081 Fragments arranged by size: 4234 2081 671 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7033 StyI C'CwwG_G Cuts at: 513 1065 3001 4472 513 Size: 552 1936 1471 3074 Fragments arranged by size: 3074 1936 1471 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 5060 5077 5230 5415 5860 325 Size: 1255 2316 1164 17 153 185 445 1498 Fragments arranged by size: 2316 1498 1255 1164 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 2162 2347 Size: 274 80 33 51 1443 120 65 185 Cuts at: 2347 2617 2747 3103 3366 3501 3656 3843 5279 Size: 270 130 356 263 135 155 187 1436 Cuts at: 5279 6991 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1436 356 274 270 263 187 185 155 138 135 130 120 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6993 7025 6993 Size: 32 7001 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7033 XbaI T'CTAG_A Cuts at: 2984 4369 2984 Size: 1385 5648 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4193 4193 Size: 7033 XhoI C'TCGA_G Cuts at: 1943 4236 1943 Size: 2293 4740 XmaI C'CCGG_G Cuts at: 1870 4358 1870 Size: 2488 4545 XmnI GAAnn'nnTTC Cuts at: 5162 5162 Size: 7033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4841 276 Size: 53 83 186 936 3307 2468 Fragments arranged by size: 3307 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI